[gmx-developers] Segfault during EM in recent git version

Berk Hess hess at cbr.su.se
Wed Oct 7 09:33:55 CEST 2009


Hi,

Steepest descents does run without too many Lincs warnings.
I don't see why you would want to run conjugate gradients on a protein
in water.
But if cg runs without virtual sites, it should also run with virtual sites.
Have you checked if the same system does miminize properly with cg without
virtual sites, but with constraints?

Berk

Jochen Hub wrote:
> Hey Berk, thanks for your patience. I have already tried with
> constraints, but then other problems occur. Now I have been trying cg
> with
>
> constraints              = all-bonds
> constraint-algorithm     = Lincs
> lincs-order              = 8
> lincs-iter               = 6
>
> As a result, the EM seems fine for 30 steps, then the rings start to
> be distorted. Within 2 steps, Tyr and Phe rings are not planar any
> more (and look pretty much like a sugar ring), and a few steps later
> the rings explode.
>
> I have played a bit with lincs-order and iter, steep instead of
> conjugate gradient, and emstep, but it did not improve things. Lincs
> warings with large angles (50-100 degree) always occur, and sometimes
> the EM breaks up because of more than 1000 lincs warnings.
>
> Jochen
>
>
>
> Berk Hess wrote:
>> Ah, the explanation is much simpler.
>> You should never use the vsite constructions in proteins without all
>> bonds constrained.
>> grompp does not check for this and it would be difficult to implement.
>> We could consider having pdb2gmx replace all bonds by constraints
>> when you ask for virtual sites.
>>
>> The actual problem is only in tetrahedral hydrogens in CH2 groups,
>> which are constructed using a non-normalized linear combinations and
>> cross product.
>> Even with all bonds constrained, this makes the hydrogen position
>> fluctuate a few percent
>> from the intended position.
>>
>> Berk
>>
>> Jochen Hub wrote:
>>  
>>> Berk Hess wrote:
>>>    
>>>> Hi,
>>>>
>>>> I think this is "normal".
>>>> It could be that with vsites you get such warnings easier, since the
>>>> distance between
>>>> the two masses is small and in EM displacement is directly
>>>> proportional
>>>> to the force
>>>> (no mass factor), so the rotation will be larger than without vsites.
>>>>
>>>>         
>>> Hi,
>>>
>>> Here's some more news on the lincs warnings. Please have a look at his
>>> shapshot series:
>>>
>>> http://xray.bmc.uu.se/~jochen/lyp.jpg
>>>
>>> Snapshot 2 (EM step 28) is right after the first one (EM step 27) and
>>> caused the first lincs warning. EM steps 1-27 look just fine.
>>> Apparently, the lincs warnings in the lysine tip are rather a side
>>> effect of wrong C-H bond lengths along the lysine side chain. A few
>>> steps later, the C-H bonds are starting to fluctuate, and finally the
>>> EM crashes (sometimes again with a Segfault), or it hangs.
>>>
>>> In addition, I am also getting weired lincs warnings with rotations of
>>> exactly (!) 90 degree:
>>>
>>> atom 1 atom 2  angle  previous, current, constraint length
>>>    247    250   90.0    0.1635   8.3257      0.1635
>>>    247    251   90.0    0.1635   7.3325      0.1635
>>>    250    251   90.0    0.0922   6.1981      0.0922
>>>    886    889   90.0    0.1583   0.6672      0.1583
>>>    886    890   90.0    0.1582   0.6672      0.1583
>>>    889    890   90.0    0.0802   0.3093      0.0802
>>>   1291   1294   90.0    0.1583   0.4725      0.1583
>>>   1291   1295   90.0    0.1582   0.4725      0.1583
>>>   1294   1295   90.0    0.0802   0.2394      0.0802
>>>   1513   1516   40.2    0.1583   0.1586      0.1583
>>>   1513   1517   39.9    0.1583   0.1578      0.1583
>>>   1516   1517   38.7    0.0802   0.0802      0.0802
>>>
>>> That does not look like coincidence, I strongly feel there is
>>> something wrong with the construction of the vsites?
>>>
>>> Jochen
>>>
>>>
>>>    
>>>> Berk
>>>>
>>>> Jochen Hub wrote:
>>>>  
>>>>      
>>>>> Hi,
>>>>>
>>>>> this fixes the Segfault, thanks! However, there are still LINCS
>>>>> warnings in the lysines, and sometimes also at isoleucines and at the
>>>>> OH-group of Tyr. Typically after 20-40 EM steps which look fine
>>>>> according to the decreasing Fmax etc. They always appear if the the
>>>>> cases with C in one cg, and the 2 heavy vsites plus one vsite
>>>>> (such as
>>>>> N) together in one cg:
>>>>>
>>>>>  1291 amber99_11     80    LYP     CE   1189    -0.0143    
>>>>> 14.026   ;
>>>>> qtot -4.006
>>>>>  1292 amber99_28     80    LYP    HE1   1190     0.1135         
>>>>> 0   ;
>>>>> qtot -3.893
>>>>>  1293 amber99_28     80    LYP    HE2   1191     0.1135         
>>>>> 0   ;
>>>>> qtot -3.779
>>>>>  1294       MNH3     80    LYP   MNZ1   1192          0     
>>>>> 8.517   ;
>>>>> qtot -3.779
>>>>>  1295       MNH3     80    LYP   MNZ2   1192          0     
>>>>> 8.517   ;
>>>>> qtot -3.779
>>>>>  1296 amber99_39     80    LYP     NZ   1192    -0.3854         
>>>>> 0   ;
>>>>> qtot -4.165
>>>>>  1297 amber99_17     80    LYP    HZ1   1193       0.34         
>>>>> 0   ;
>>>>> qtot -3.825
>>>>>  1298 amber99_17     80    LYP    HZ2   1194       0.34         
>>>>> 0   ;
>>>>> qtot -3.485
>>>>>  1299 amber99_17     80    LYP    HZ3   1195       0.34         
>>>>> 0   ;
>>>>> qtot -3.145
>>>>>
>>>>> Where the warning looks like that:
>>>>>
>>>>> bonds that rotated more than 30 degrees:
>>>>> atom 1 atom 2  angle  previous, current, constraint length
>>>>>   1291   1294   36.8    0.1583   0.1576      0.1583   -> C--MNH3
>>>>>   1291   1295   37.6    0.1583   0.1587      0.1583   -> C--MNH3
>>>>>   1294   1295   35.4    0.0802   0.0805      0.0802   -> MNH3-MNH3
>>>>>
>>>>> Cheers,
>>>>> Jochen
>>>>>
>>>>>
>>>>> hess at sbc.su.se wrote:
>>>>>           
>>>>>> Ah, this is in the vsite fix I made for the bug you
>>>>>> reported previously.
>>>>>> This code is quite obviously incorrect.
>>>>>> But I realize now it can be done simpler.
>>>>>> if (atom[a].ptype != eptVSite || pbc_set[a]) {
>>>>>> can be replaced by
>>>>>> if (pbc_set[a]) {
>>>>>>
>>>>>> This will certainly solve your last crash.
>>>>>> Just to be sure, could you check that this still fixes
>>>>>> the bug you reported previously?
>>>>>>
>>>>>> Berk
>>>>>>
>>>>>>
>>>>>>  
>>>>>>               
>>>>>>> Sorry, here a few more details:
>>>>>>>
>>>>>>> (gdb) list
>>>>>>> 1498            }
>>>>>>> 1499            if (vsite_pbc_f[vsi] == -1) {
>>>>>>> 1500              /* Check if this is the first processed atom of a
>>>>>>> vsite only cg */
>>>>>>> 1501              bViteOnlyCG_and_FirstAtom = TRUE;
>>>>>>> 1502              for(a=cgs->index[cg_v]; a<cgs->index[cg_v+1];
>>>>>>> a++) {
>>>>>>> 1503                if (atom[a].ptype != eptVSite || pbc_set[a]) {
>>>>>>> 1504                  bViteOnlyCG_and_FirstAtom = FALSE;
>>>>>>> 1505                  break;
>>>>>>> 1506                }
>>>>>>> 1507              }
>>>>>>>
>>>>>>> The problem is that the atom[a=0] is not defined.
>>>>>>>
>>>>>>> Jochen
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>> Berk Hess wrote:
>>>>>>>                      
>>>>>>>> Jochen Hub wrote:
>>>>>>>>
>>>>>>>>                            
>>>>>>>>> Berk Hess wrote:
>>>>>>>>>
>>>>>>>>>                                  
>>>>>>>>>> Did you use cut-off's?
>>>>>>>>>> New all-versus-all loops have been added for simulations without
>>>>>>>>>> cut-off's.
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>>                                           
>>>>>>>>> Yes, for both coulomb and vdw:
>>>>>>>>>
>>>>>>>>> coulombtype              = Cut-off
>>>>>>>>> vdw-type                 = Cut-off
>>>>>>>>>
>>>>>>>>> Jochen
>>>>>>>>>
>>>>>>>>>                                     
>>>>>>>> Sorry, maybe my question was a but unclear.
>>>>>>>> Do you have finite cut-off lengths, or do you have pbc=no and
>>>>>>>> cut-off's
>>>>>>>> set to 0 (infinity).
>>>>>>>>
>>>>>>>> Berk
>>>>>>>>
>>>>>>>>                            
>>>>>>>>>> Berk
>>>>>>>>>>
>>>>>>>>>> Jochen Hub wrote:
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>>                                        
>>>>>>>>>>> Hi Berk,
>>>>>>>>>>>
>>>>>>>>>>> yes, I have always reconfigured with distclean and everything.
>>>>>>>>>>> And
>>>>>>>>>>> checked that no CC variables or similar were messing up
>>>>>>>>>>> something. I
>>>>>>>>>>> could well imagine that the problem is again due to virtual
>>>>>>>>>>> sites
>>>>>>>>>>> which I have in my structure.
>>>>>>>>>>>
>>>>>>>>>>> In addition, I am getting LINCS warnings during EM with the 405
>>>>>>>>>>> from
>>>>>>>>>>> a
>>>>>>>>>>> few weeks ago, but *not* related to the bug you fixed recently
>>>>>>>>>>> (with
>>>>>>>>>>> CGs with only vsites). These lincs warnings appear typically in
>>>>>>>>>>> lysines, somewhere between the last carbon and the NH3 in the
>>>>>>>>>>> lysine
>>>>>>>>>>> chain. Noteworthy, the warnings did not appear with steepest
>>>>>>>>>>> descent,
>>>>>>>>>>> but with conjugate gradient. Higher lincs-order did not
>>>>>>>>>>> help, but
>>>>>>>>>>> even
>>>>>>>>>>> increased the large changes in angles. For example:
>>>>>>>>>>>
>>>>>>>>>>> atom 1 atom 2  angle  previous, current, constraint length
>>>>>>>>>>>    886    889   34.3    0.1583   0.1582      0.1583
>>>>>>>>>>>    886    890   33.8    0.1583   0.1580      0.1583
>>>>>>>>>>>    889    890   34.0    0.0802   0.0805      0.0802
>>>>>>>>>>>   1291   1294   33.8    0.1583   0.1578      0.1583
>>>>>>>>>>>   1291   1295   34.3    0.1583   0.1586      0.1583
>>>>>>>>>>>   1294   1295   32.3    0.0802   0.0803      0.0802
>>>>>>>>>>>
>>>>>>>>>>> where the respective atoms are:
>>>>>>>>>>>   886 amber99_11     55    LYP     CE    822    -0.0143   
>>>>>>>>>>> 14.026
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.006
>>>>>>>>>>>   887 amber99_28     55    LYP    HE1    823   
>>>>>>>>>>> 0.1135          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.893
>>>>>>>>>>>   888 amber99_28     55    LYP    HE2    824   
>>>>>>>>>>> 0.1135          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.779
>>>>>>>>>>>   889       MNH3     55    LYP   MNZ1    825          0    
>>>>>>>>>>> 8.517
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.779
>>>>>>>>>>>   890       MNH3     55    LYP   MNZ2    825          0    
>>>>>>>>>>> 8.517
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.779
>>>>>>>>>>>   891 amber99_39     55    LYP     NZ    825  
>>>>>>>>>>> -0.3854          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.165
>>>>>>>>>>>   892 amber99_17     55    LYP    HZ1    826     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.825
>>>>>>>>>>>   893 amber99_17     55    LYP    HZ2    827     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.485
>>>>>>>>>>>   894 amber99_17     55    LYP    HZ3    828     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -2.145
>>>>>>>>>>>
>>>>>>>>>>> The atoms around nr 1291 are also the tip of  a lysine side
>>>>>>>>>>> chain.
>>>>>>>>>>>  1291 amber99_11     80    LYP     CE   1189    -0.0143   
>>>>>>>>>>> 14.026
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -4.006
>>>>>>>>>>>  1292 amber99_28     80    LYP    HE1   1190   
>>>>>>>>>>> 0.1135          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.893
>>>>>>>>>>>  1293 amber99_28     80    LYP    HE2   1191   
>>>>>>>>>>> 0.1135          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.779
>>>>>>>>>>>  1294       MNH3     80    LYP   MNZ1   1192          0    
>>>>>>>>>>> 8.517
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.779
>>>>>>>>>>>  1295       MNH3     80    LYP   MNZ2   1192          0    
>>>>>>>>>>> 8.517
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.779
>>>>>>>>>>>  1296 amber99_39     80    LYP     NZ   1192  
>>>>>>>>>>> -0.3854          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -4.165
>>>>>>>>>>>  1297 amber99_17     80    LYP    HZ1   1193     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.825
>>>>>>>>>>>  1298 amber99_17     80    LYP    HZ2   1194     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.485
>>>>>>>>>>>  1299 amber99_17     80    LYP    HZ3   1195     
>>>>>>>>>>> 0.34          0
>>>>>>>>>>> ;
>>>>>>>>>>> qtot -3.145
>>>>>>>>>>>
>>>>>>>>>>> Jochne
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>> Berk Hess wrote:
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>>                                              
>>>>>>>>>>>> Hi,
>>>>>>>>>>>>
>>>>>>>>>>>> Did you try to rerun configure and recompile everything?
>>>>>>>>>>>> I just compared EM to an older version and got exactly the
>>>>>>>>>>>> same
>>>>>>>>>>>> results.
>>>>>>>>>>>>
>>>>>>>>>>>> Berk
>>>>>>>>>>>>
>>>>>>>>>>>> Jochen Hub wrote:
>>>>>>>>>>>>
>>>>>>>>>>>>
>>>>>>>>>>>>
>>>>>>>>>>>>                                                    
>>>>>>>>>>>>> Hi Ran,
>>>>>>>>>>>>>
>>>>>>>>>>>>> did you also have these problems only during energy
>>>>>>>>>>>>> minimization? I
>>>>>>>>>>>>> asking since I want to use the present git for longer
>>>>>>>>>>>>> production
>>>>>>>>>>>>> runs?
>>>>>>>>>>>>>
>>>>>>>>>>>>> Best,
>>>>>>>>>>>>> Jochen
>>>>>>>>>>>>>
>>>>>>>>>>>>>
>>>>>>>>>>>>>
>>>>>>>>>>>>> Ran Friedman wrote:
>>>>>>>>>>>>>
>>>>>>>>>>>>>
>>>>>>>>>>>>>                                                          
>>>>>>>>>>>>>> Hi,
>>>>>>>>>>>>>>
>>>>>>>>>>>>>> I'm fighting these issues as well.
>>>>>>>>>>>>>>
>>>>>>>>>>>>>> With the new git version I either get a segfault or really
>>>>>>>>>>>>>> unrealistic
>>>>>>>>>>>>>> structures. For example, I saw that a hydrogen bound to
>>>>>>>>>>>>>> nitrogen
>>>>>>>>>>>>>> near a
>>>>>>>>>>>>>> C-terminal carboxylate group was located at the same place
>>>>>>>>>>>>>> of a
>>>>>>>>>>>>>> carboxylate oxygen (!) following minimisation. As this
>>>>>>>>>>>>>> happened in
>>>>>>>>>>>>>> more
>>>>>>>>>>>>>> than one structure I guess something is broken. It's as if
>>>>>>>>>>>>>> minimisation
>>>>>>>>>>>>>> doesn't take the LJ parameters into account.
>>>>>>>>>>>>>>
>>>>>>>>>>>>>> Ran.
>>>>>>>>>>>>>>
>>>>>>>>>>>>>> Jochen Hub wrote:
>>>>>>>>>>>>>>
>>>>>>>>>>>>>>
>>>>>>>>>>>>>>
>>>>>>>>>>>>>>                                      
>>>>>>>>>>>>>>                          
>>>>>>>>>>>>>>> Hi,
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> mdrun of the present git version stops during energy
>>>>>>>>>>>>>>> minimization in
>>>>>>>>>>>>>>> Segfaul/Bus error on my Mac. Gromacs 405 from a few
>>>>>>>>>>>>>>> weeks ago
>>>>>>>>>>>>>>> works
>>>>>>>>>>>>>>> fine with the same tpr and the same configure options. gdb
>>>>>>>>>>>>>>> reports
>>>>>>>>>>>>>>> some error in the assembly loops, I am not sure what the
>>>>>>>>>>>>>>> error
>>>>>>>>>>>>>>> means
>>>>>>>>>>>>>>> however:
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> gcc version 4.0.1 (Apple Inc. build 5490)
>>>>>>>>>>>>>>> -------------------------------------------
>>>>>>>>>>>>>>> Program received signal EXC_BAD_INSTRUCTION, Illegal
>>>>>>>>>>>>>>> instruction/operand.
>>>>>>>>>>>>>>> _nb_kernel_ia32_3dnow_test_asm () at
>>>>>>>>>>>>>>> nb_kernel_ia32_3dnow_test_asm.s:23
>>>>>>>>>>>>>>> 23      nb_kernel_ia32_3dnow_test_asm.s: No such file or
>>>>>>>>>>>>>>> directory.
>>>>>>>>>>>>>>>        in nb_kernel_ia32_3dnow_test_asm.s
>>>>>>>>>>>>>>> (gdb) bt
>>>>>>>>>>>>>>> #0  _nb_kernel_ia32_3dnow_test_asm () at
>>>>>>>>>>>>>>> nb_kernel_ia32_3dnow_test_asm.s:23
>>>>>>>>>>>>>>> #1  0x002682a0 in nb_kernel_ia32_3dnow_test
>>>>>>>>>>>>>>> (log=0xa08834c0) at
>>>>>>>>>>>>>>> nb_kernel_ia32_3dnow.c:186
>>>>>>>>>>>>>>> #2  0x0026831f in nb_kernel_setup_ia32_3dnow
>>>>>>>>>>>>>>> (log=0xa08834c0,
>>>>>>>>>>>>>>> list=0x804200) at nb_kernel_ia32_3dnow.c:212
>>>>>>>>>>>>>>> #3  0x00268bb2 in gmx_setup_kernels (fplog=0xa08834c0) at
>>>>>>>>>>>>>>> nonbonded.c:248
>>>>>>>>>>>>>>> #4  0x00057899 in init_forcerec (fp=0xa08834c0,
>>>>>>>>>>>>>>> fr=0x81d000,
>>>>>>>>>>>>>>> fcd=0x503130, ir=0x801a00, mtop=0x501860, cr=0x500330,
>>>>>>>>>>>>>>> box=0xbfffd76c,
>>>>>>>>>>>>>>> bMolEpot=0, tabfn=0x500590 "table.xvg", tabpfn=0x5005b0
>>>>>>>>>>>>>>> "tablep.xvg",
>>>>>>>>>>>>>>> tabbfn=0x5005d0 "table.xvg", bNoSolvOpt=0,
>>>>>>>>>>>>>>> print_force=-1) at
>>>>>>>>>>>>>>> force.c:1436
>>>>>>>>>>>>>>> #5  0x00012529 in mdrunner (fplog=0xa08834c0, cr=0x500330,
>>>>>>>>>>>>>>> nfile=27,
>>>>>>>>>>>>>>> fnm=0x2a06c0, bVerbose=1, bCompact=1, ddxyz=0xbfffe8e0,
>>>>>>>>>>>>>>> dd_node_order=1, rdd=0, rconstr=0, dddlb_opt=0x2801b1
>>>>>>>>>>>>>>> "auto",
>>>>>>>>>>>>>>> dlb_scale=0.800000012, ddcsx=0x0, ddcsy=0x0, ddcsz=0x0,
>>>>>>>>>>>>>>> nstepout=100,
>>>>>>>>>>>>>>> ed=0x0, repl_ex_nst=0, repl_ex_seed=-1, pforce=-1,
>>>>>>>>>>>>>>> cpt_period=15,
>>>>>>>>>>>>>>> max_hours=-1, Flags=7168) at md.c:305
>>>>>>>>>>>>>>> #6  0x00018ce0 in main (argc=6, argv=0xbfffe94c) at
>>>>>>>>>>>>>>> mdrun.c:496
>>>>>>>>>>>>>>> Current language:  auto; currently asm
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> Newer gccs (4.2 or 4.4) did not help. Compiling in 64bit
>>>>>>>>>>>>>>> did
>>>>>>>>>>>>>>> not
>>>>>>>>>>>>>>> help
>>>>>>>>>>>>>>> either.
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> Any suggestions?
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> Thanks a lot,
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>> Jochen
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>>
>>>>>>>>>>>>>>>                                            
>>>>>>>>>>>>>>>                             
>>>>>>>>>>>>>> _______________________________________________
>>>>>>>>>>>>>> gmx-developers mailing list
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>>>>>>>>>>>>>>
>>>>>>>>>>>>>>
>>>>>>>>>>>>>>                                        
>>>>>>>>>>>>>>                           
>>>>>>>>>>>>>                                                             
>>>>>>>>>>>> _______________________________________________
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>>>>>>>>>>>>                                                       
>>>>>>>>>>>                                                 
>>>>>>>>>> _______________________________________________
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>>>>>>>>>>                                           
>>>>>>>> _______________________________________________
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>>>>>>>>
>>>>>>>>                               
>>>>>>> -- 
>>>>>>> ---------------------------------------------------
>>>>>>> Dr. Jochen Hub
>>>>>>> Molecular Biophysics group
>>>>>>> Dept. of Cell & Molecular Biology
>>>>>>> Uppsala University. Box 596, 75124 Uppsala, Sweden.
>>>>>>> Phone: +46-18-4714451 Fax: +46-18-511755
>>>>>>> ---------------------------------------------------
>>>>>>>
>>>>>>> _______________________________________________
>>>>>>> gmx-developers mailing list
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>>>>>>>                         
>>>>>> _______________________________________________
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>>>>>             
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