[gmx-users] AFM pulling: how to determine the spring constant?

[Mario Orsi]

Hi guys,

i'm stretchin' a protein with gmx; i'm not sure about
the value of the spring constant... how to set it
right? 
I've read a couple of papers where they describe SMD
pulling, and they chose the spring constant from this
formula:

k=10*Kb*T/(dx)^2

what is this equation about? where can I find sth that
explains the theory behind (the formula above) a
correct setting of k?
thanks a lot
mario orsi

Burns: "Simpson, I want to be loved." 
Homer: "Well ... I'm gonna need some beer ..." 


	

	
		
____________________________________________________________
Yahoo! Companion - Scarica gratis la toolbar di Ricerca di Yahoo! 
http://companion.yahoo.it