[gmx-users] Different coulomb energies of protein by Cut-off and PME

David spoel at xray.bmc.uu.se
Thu Apr 28 19:57:14 CEST 2005


On Thu, 2005-04-28 at 10:49 -0500, Zhiyong Zhang wrote:
> Hi,
> 
> Thanks for your reply!
> 
> The protein has 51 residues and 530 atoms. I included explicit waters in
> the simulations. The following are coulomb energies of the total system
> from Cut-off and PME, respectively.
> 
> Cut-off:
> 
> Energy                      Average       RMSD     Fluct.      Drift 
> Tot-Drift
> -------------------------------------------------------------------------------
> Coulomb-14                 5793.36    39.3909    39.1433 -0.00305485  
> -15.2743
> Coulomb (SR)                -248203    671.972    668.068  -0.050111  
> -250.555
> Coulomb (LR)               -3725.48    425.932    418.264  0.0557401   
> 278.701
> Total                       -246136         --         --   --        
> --
> 
> PME:
> 
> Energy                      Average       RMSD     Fluct.      Drift 
> Tot-Drift
> -------------------------------------------------------------------------------
> Coulomb-14                  5800.09    42.6203    42.4826 -0.00197631  
> -11.8579
> Coulomb (SR)                -215201    573.112    573.087 -0.00309615  
> -18.5769
> Coulomb (LR)               -34776.9    45.6159    45.6125 0.00032001   
> 1.92006
> Total                       -244178         --         --     --        
> --
> 
> If you need any other information, please let me know. I noticed that,
> the coulomb energies of the whole system are similar, why it is
> different when I only considered protein?
Because protein-protein energy is implicitly contained in the PME
energy. See list archive for splitting the energy in groups.

> 
> Regards
> Zhiyong
> 
> On Thu, 2005-04-28 at 01:27, David wrote:
> > On Wed, 2005-04-27 at 10:43 -0500, Zhiyong Zhang wrote:
> > > Dear gmx users,
> > > 
> > > For the same protein, I performed a MD simulation using Cut-off and PME,
> > > respectively. The following are parameters:
> > > 
> > You are using very different setup and give too little information.
> > How large is the protein?
> > Was there solvent in the simulations?
> > If so where is the solvent energy?
> > 
> > 
> > > For Cut-off:
> > > 
> > > ; NEIGHBORSEARCHING PARAMETERS =
> > > ; nblist update frequency =
> > > nstlist                  = 5
> > > ; ns algorithm (simple or grid) =
> > > ns_type                  = grid
> > > ; Periodic boundary conditions: xyz or no =
> > > pbc                      = xyz
> > > ; nblist cut-off         =
> > > rlist                    = 1.0
> > > domain-decomposition     = no
> > > 
> > > ; OPTIONS FOR ELECTROSTATICS AND VDW =
> > > ; Method for doing electrostatics =
> > > coulombtype              = Cut-off
> > > rcoulomb-switch          = 0
> > > rcoulomb                 = 1.4
> > > ; Dielectric constant (DC) for cut-off or DC of reaction field =
> > > epsilon-r                = 1
> > > ; Method for doing Van der Waals =
> > > vdw-type                 = Cut-off
> > > ; cut-off lengths        =
> > > rvdw-switch              = 0
> > > rvdw                     = 1.0
> > > ; Apply long range dispersion corrections for Energy and Pressure =
> > > DispCorr                 = No
> > > ; Spacing for the PME/PPPM FFT grid =
> > > fourierspacing           = 0.12
> > > ; FFT grid size, when a value is 0 fourierspacing will be used =
> > > fourier_nx               = 0
> > > fourier_ny               = 0
> > > fourier_nz               = 0
> > > ; EWALD/PME/PPPM parameters =
> > > pme_order                = 4
> > > ewald_rtol               = 1e-05
> > > ewald_geometry           = 3d
> > > epsilon_surface          = 0
> > > optimize_fft             = no
> > > 
> > > For PME:
> > > 
> > > ; NEIGHBORSEARCHING PARAMETERS =
> > > ; nblist update frequency =
> > > nstlist                  = 5
> > > ; ns algorithm (simple or grid) =
> > > ns_type                  = grid
> > > ; Periodic boundary conditions: xyz or no =
> > > pbc                      = xyz
> > > ; nblist cut-off         =
> > > rlist                    = 0.9
> > > domain-decomposition     = no
> > > 
> > > ; OPTIONS FOR ELECTROSTATICS AND VDW =
> > > ; Method for doing electrostatics =
> > > coulombtype              = PME
> > > rcoulomb-switch          = 0
> > > rcoulomb                 = 0.9
> > > ; Dielectric constant (DC) for cut-off or DC of reaction field =
> > > epsilon-r                = 1
> > > ; Method for doing Van der Waals =
> > > vdw-type                 = Cut-off
> > > ; cut-off lengths        =
> > > rvdw-switch              = 0
> > > rvdw                     = 1.4
> > > ; Apply long range dispersion corrections for Energy and Pressure =
> > > DispCorr                 = No
> > > ; Spacing for the PME/PPPM FFT grid =
> > > fourierspacing           = 0.12
> > > ; FFT grid size, when a value is 0 fourierspacing will be used =
> > > fourier_nx               = 0
> > > fourier_ny               = 0
> > > fourier_nz               = 0
> > > ; EWALD/PME/PPPM parameters =
> > > pme_order                = 4
> > > ewald_rtol               = 1e-05
> > > ewald_geometry           = 3d
> > > epsilon_surface          = 0
> > > optimize_fft             = no
> > > 
> > > After simulations, I calculated the coulomb energies in the protein.
> > > 
> > > Cut-off:
> > > 
> > > Energy                      Average       RMSD     Fluct.      Drift
> > > Tot-Drift
> > > -------------------------------------------------------------------------------
> > > Coul-SR:Protein-Protein     -8733.4    314.267    287.225 -0.0883592  
> > > -441.796
> > > Coul-LR:Protein-Protein   -0.280069    254.458    250.858  0.0295494   
> > > 147.747
> > > Coul-14:Protein-Protein     5793.36    39.3909    39.1433 -0.00305485  
> > > -15.2743
> > > Total                      -2940.32         --         --     --        
> > > --
> > > 
> > > PME:
> > > 
> > > Energy                      Average       RMSD     Fluct.      Drift 
> > > Tot-Drift
> > > -------------------------------------------------------------------------------
> > > Coul-SR:Protein-Protein    -1314.89    79.1684    75.3171 -0.0140827  
> > > -84.4963
> > > Coul-LR:Protein-Protein         0          0          0       0         
> > > 0
> > > Coul-14:Protein-Protein     5800.09    42.6203    42.4826 -0.00197631  
> > > -11.8579
> > > Total                        4485.2         --         --     --        
> > > --
> > > 
> > > I noticed that, the total coulomb energies in the protein by Cut-off and
> > > PME are quite different. Would you please explain it? Thanks!
> > > 
> > > Best wishes
> > > Zhiyong Zhang
> > > -- 
> > > Postdoctoral Fellow,
> > > School of Health Information Sciences,
> > > University of Texas Health Science Center - Houston,
> > > 7000 Fannin St., suite 1480,
> > > Houston, TX 77030, U.S.A.
> > > email: Zhiyong.Zhang at uth.tmc.edu
> > > http://www.biomachina.org/people/zhang/
> > > 
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-- 
David.
________________________________________________________________________
David van der Spoel, PhD, Assoc. Prof., Molecular Biophysics group,
Dept. of Cell and Molecular Biology, Uppsala University.
Husargatan 3, Box 596,          75124 Uppsala, Sweden
phone:  46 18 471 4205          fax: 46 18 511 755
spoel at xray.bmc.uu.se    spoel at gromacs.org   http://xray.bmc.uu.se/~spoel
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