[gmx-users] problem while entering a new residue in ffgmx.rtp file

Rahul Karyappa r.karyappa at ncl.res.in
Thu Aug 18 11:39:04 CEST 2005


Dear all,

   I have a polyethylene.pdb file for 10 repeat units of
polyethylene. I want to define atoms and bonds for residue PE in the
ffgmx.rtp file. When I enter all the atoms as it is in the .pdb file
and define the bonds, I am able to run pdb2gmx.  But  if I
want to run pdb2gmx on higher no. of repeat units then
it will be time-consuming to define each atom and bond. Can I add atoms
and bonds for one repeat unit
and use it for any no. of length of the polymer?  My .pdb file
looks like as follows:


REMARK 

REMARK 

REMARK  This file was generated by PRODRG version 050728.0532

REMARK  PRODRG written/copyrighted by Daan van Aalten

REMARK 

REMARK  Questions/comments to dava at davapc1.bioch.dundee.ac.uk

REMARK 

REMARK  When using this software in a publication, cite:

REMARK  A. W. Schuettelkopf and D. M. F. van Aalten (2004).

REMARK  PRODRG - a tool for high-throughput crystallography

REMARK  of protein-ligand complexes.

REMARK  Acta Crystallogr. D60, 1355--1363.

REMARK 

REMARK 

HETATM    1  CAA PE    
1       1.216   1.219
-0.167  1.00
20.00           
C

HETATM    2 1HAA PE    
1       1.327   0.277
-0.484  1.00
20.00           
H

HETATM    3 2HAA PE    
1       0.787 
1.218   0.736  1.00
20.00           
H

HETATM    4 3HAA PE    
1       0.637   1.721
-0.810  1.00
20.00           
H

HETATM    5  CAB PE    
1       2.587   1.894
-0.081  1.00
20.00           
C

HETATM    6 1HAB PE    
1       3.006   1.880
-0.989  1.00
20.00           
H

HETATM    7 2HAB PE    
1       3.157 
1.377   0.558  1.00
20.00           
H

HETATM    8  CAC PE    
1       2.514 
3.351   0.400  1.00
20.00           
C

HETATM    9 1HAC PE    
1       2.094 
3.363   1.307  1.00
20.00           
H

HETATM   10 2HAC PE    
1       1.943   3.866
-0.239  1.00
20.00           
H

HETATM   11  CAD PE    
1       3.884 
4.039   0.490  1.00
20.00           
C

HETATM   12 1HAD PE    
1       4.305   4.027
-0.417  1.00
20.00           
H

HETATM   13 2HAD PE    
1       4.455 
3.525   1.130  1.00
20.00           
H

HETATM   14  CAE PE    
1       3.806 
5.496   0.970  1.00
20.00           
C

HETATM   15 1HAE PE    
1       3.386 
5.508   1.877  1.00
20.00           
H

HETATM   16 2HAE PE    
1       3.234 
6.010   0.330  1.00
20.00           
H

HETATM   17  CAF PE    
1       5.176 
6.185   1.059  1.00
20.00           
C

HETATM   18 1HAF PE    
1       5.597 
6.171   0.152  1.00
20.00           
H

HETATM   19 2HAF PE    
1       5.747 
5.672   1.700  1.00
20.00           
H

HETATM   20  CAG PE    
1       5.098 
7.643   1.537  1.00
20.00           
C

HETATM   21 1HAG PE    
1       4.678 
7.658   2.445  1.00
20.00           
H

HETATM   22 2HAG PE    
1       4.528 
8.157   0.897  1.00
20.00           
H

HETATM   23  CAH PE    
1       6.474 
8.325   1.623  1.00
20.00           
C

HETATM   24 1HAH PE    
1       6.871 
8.339   0.705  1.00
20.00           
H

HETATM   25 2HAH PE    
1       7.053 
7.779   2.229  1.00
20.00           
H

HETATM   26  CAI PE    
1       6.452 
9.768   2.156  1.00
20.00           
C

HETATM   27 1HAI PE    
1       7.398  10.074 
2.266  1.00
20.00           
H

HETATM   28 2HAI PE    
1       5.997 
9.764   3.046  1.00
20.00           
H

HETATM   29  CAJ PE    
1       5.727  10.771 
1.253  1.00
20.00           
C

HETATM   30 1HAJ PE    
1       5.759  11.679 
1.670  1.00
20.00           
H

HETATM   31 2HAJ PE    
1       6.174  10.799 
0.359  1.00
20.00           
H

HETATM   32 3HAJ PE    
1       4.774  10.490 
1.139  1.00
20.00           
H

CONECT    1    2    3    4    5

CONECT    2    1

CONECT    3    1

CONECT    4    1

CONECT    5    1    6    7    8

CONECT    6    5

CONECT    7    5

CONECT    8    5    9   10   11

CONECT    9    8

CONECT   10    8

CONECT   11    8   12   13   14

CONECT   12   11

CONECT   13   11

CONECT   14   11   15   16   17

CONECT   15   14

CONECT   16   14

CONECT   17   14   18   19   20

CONECT   18   17

CONECT   19   17

CONECT   20   17   21   22   23

CONECT   21   20

CONECT   22   20

CONECT   23   20   24   25   26

CONECT   24   23

CONECT   25   23

CONECT   26   23   27   28   29

CONECT   27   26

CONECT   28   26

CONECT   29   26   30   31   32

CONECT   30   29

CONECT   31   29

CONECT   32   29

END


-This is how I defined the residue PE in the .rtp file:


[ atoms ]

   CAA   C   -0.2913     0

  HAA1   H    0.0000     0

  HAA2     H    0.1450     0

  HAA3   H    0.1450     0

   CAB   C   -0.2888     0

  HAB1   H    0.1451     0

  HAB2   H    0.1451     0

   CAC   C   -0.2913     0

  HAC1   H    0.1450     0

  HAC2   H    0.1450     0

   CAD   C   -0.2888     0

  HAD1   H    0.1451     0

  HAD2   H    0.1451     0

   CAE   C   -0.2913     0

  HAE1   H    0.1450     0

  HAE2   H    0.1450     0

   CAF   C   -0.2888     0

  HAF1   H    0.1451     0

  HAF2   H    0.1451     0

   CAG   C   -0.2913     0

  HAG1   H    0.1450     0

  HAG2   H    0.1450     0

   CAH   C   -0.2888     0

  HAH1   H    0.1451     0

  HAH2   H    0.1451     0

   CAI   C   -0.2913     0

  HAI1   H    0.1450     0

  HAI2   H    0.1450     0

   CAJ   C   -0.2888     0

  HAJ1   H    0.0000     0

  HAJ2   H    0.1451     0

  HAJ3   H    0.1451     0

 [ bonds ]

   CAA  1HAA

   CAA  2HAA

   CAA  3HAA

   CAA   CAB

   CAB  1HAB

   CAB  2HAB

   CAB   CAC

   CAC  1HAC

   CAC  2HAC

   CAC   CAD

   CAD  1HAD

   CAD  2HAD 

   CAD   CAE

   CAE  1HAE

   CAE  2HAE

   CAE   CAF

   CAF  1HAF

   CAF  2HAF

   CAF   CAG

   CAG  1HAG

   CAG  2HAG

   CAG   CAH

   CAH  1HAH

   CAH  2HAH

   CAH   CAI

   CAI  1HAI

   CAI  2HAI

   CAI   CAJ

   CAJ  1HAJ

   CAJ  2HAJ

   CAJ  3HAJ


After defining them in .rtp file like this, I got the .gro and .top
file. But when I ran grompp it gave me error Fatal Error: No default
bond/angle/dihedral types, using zeroes.


Can anyone have suggestions for this?

Thank you in advance.


Rahul Karyappa

NCL, Pune

India







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