[gmx-users] Methanol SOL :Number of solvent molecules = 0

sharada sharada at ccmb.res.in
Tue Dec 11 06:29:12 CET 2007



    
        
            
Dear Dr. Justin A. Lemkul
Thank you for your reply. I tried adding the line 'SOL  522 ' at the end of the .top file 
but  genbox is deleting the line. 
------------------------------------------------------------
Output configuration contains 1076 atoms in 341 residues
Volume                 :     24.1334 (nm^3)
Density                :     720.413 (g/l)
Number of SOL molecules:      0
Processing topology
Removing line #480 'SOL                 522' from topology file (agg2.top)
-------------------------------------------------------------------------------------------
Thankyou Dr. Xavier Periole for indicating that the bonds and angle information does not match with the original Gromos distributed file.  I am inserting methanol.itp file  which came with gromacs distribution.  I downloaded the methanol216.gro.gz from gromacs site.  Should I change  the bond values in the itp file? But it still does not solve the problem of 'SOL molecules'. Since .top file indicates "#include "spc.itp". So if I change it to #methanol.itp"   it should work.
-----------------------------------------------
[root at ccmb top]# more methanol.itp
#ifndef _FF_GROMOS96
[ atomtypes ]
;   type      mass    charge    ptype       c6            c12
    OMET    15.999    -0.69     A       2.6169e-3      2.5231e-6
      OW        15.999    -0.82     A       2.6170e-3      2.6330e-6
    CMET    15.035     0.29     A       8.8758e-3     17.8426e-6
       H          1.008     0.4      A       0.0            0.0
      HW        1.008     0.41     A       0.0            0.0
#endif
[ moleculetype ]
; name  nrexcl
Methanol        2
[ atoms ]
;   nr  type    resnr   residu  atom    cgnr    charge  mass
#ifdef _FF_GROMOS96
1       CMET     1       MeOH    Me1     1        0.176 15.035
2       OMET     1       MeOH    O2        1       -0.574 15.999
3       H        1             MeOH    H3      1        0.398  1.008
#else
1       CMET     1         MeOH    Me1     1 Regards 
 sharada       0.29  15.035
2       OMET     1          MeOH    O2      1       -0.69  15.999
3               H        1       MeOH    H3      1        0.40   1.008
#endif
[ bonds ]
;  ai  aj funct           c0           c1
1       2       1          0.13600     376560.
2       3       1          0.10000     313800.
[ angles ]
;  ai    aj    ak       funct   c0      c1
    1     2     3       1       108.53  397.5
Regards 
sharada 
(CCMB)
-------------------------------------------------------------------------------------------------------------------------------------------------
-- Original Message --
From: "Justin A. Lemkul" <jalemkul at vt.edu>
To: Discussion list for GROMACS users <gmx-users at gromacs.org>
Date: Mon, 10 Dec 2007 06:36:56 -0500
Subject: Re: [gmx-users] Methanol SOL :Number of solvent molecules = 0
Quoting sharada <sharada at ccmb.res.in>:
>  526MeOH   Me1 1629   2.760   3.368   2.738
>   526MeOH    O2 1630   2.695   3.423   2.632
>   526MeOH    H3 1631   2.615   3.371   2.606
>   527MeOH   Me1 1632   0.288   3.224   2.925
>   527MeOH    O2 1633   0.194   3.156   2.996
>   527MeOH    H3 1634   0.212   3.059   2.983
>   528MeOH   Me1 1635   2.506   3.095   2.599
>   528MeOH    O2 1636   2.457   3.218   2.567
>   528MeOH    H3 1637   2.390   3.204   2.494
>    3.30500   3.58500   2.95900
> Why does it say there are no SOL molecules.  Where exactly am I going wrong ?
> Kindly advice me. Thankyou for your patience.
> sharada
>
My guess would be that it is because there are no molecules named SOL in your
output.  Gromacs uses SOL for water, and since your system lacks water, genbox
is accurately telling you there are no "SOL" within the output.
Note the line above where genbox is telling you that it added 522 molecules, and
add this information into your .top file.
-Justin
========================================
Justin A. Lemkul
Graduate Research Assistant
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul at vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin/
========================================
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