[gmx-users] Regarding neutralization of lipid bilayer

[Mark Abraham]

> Dear gmx users,
>
>         I built POPE+POPG (total 128 lipid molecules)
> lipid bilayer in 3:1 ratio. POPE has neutral charge
> and POPG has one unit of negative charge.To this lipid
> molecules 3941 water molecules are added.
> While creating bilayer.tpr file, it is giving non-zero
> total charge: -4.534399e+01 related information was
> shown below.

This suggests your [molecule] definitions for these POPE and POPG don't
agree with what you think they should be :-) Check the totals of their
charges.

Mark