[gmx-users] Re: gmx-users Digest, Vol 39, Issue 56

ann rose annrose.johns at gmail.com
Tue Jul 17 14:13:13 CEST 2007


>
> ------------------------------
>
> Message: 5
> Date: Mon, 16 Jul 2007 16:39:29 +0200
> From: "Jeroen van Bemmelen" < J.J.M.vanBemmelen at tnw.tudelft.nl>
> Subject: [gmx-users] Regarding polymer equillibration (pressure
>         changes)
> To: gmx-users at gromacs.org
> Message-ID: < 469B9F40.30804.32BEF5D at J.J.M.vanBemmelen.tnw.tudelft.nl>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Ann,
>
> >  So my first question is
> >
> >  wether this can be possible or not? or am I doing something wrong?
> >  if so, then how can i rectify the same error?
>
> I think you should definitely use 'editconf -density' to rescale your
> simulation box to the right density. editconf automatically rescales
> the box vectors AND the coordinates, so you won't have the problem of
> atom coordinates suddenly being larger than the box vectors.
>
> But anyhow it seems a bit weird to me that you're coordinates are so
> much larger than your box vectors (4.3 versus 3.4 nm). Perhaps this
> is related to the long molecule chains in combination with pbc? I'm
> not sure, maybe one of the developers can comment on this.
>
>
> > I think the fluctuations are okay and admittable.but the change in
> pressure
> > averages make me confused.
> >
> > If I am right, at equillibrium all the observables (temp, pressure,
> density)
> > should average around a steady value with some fluctuations. but the
> change
> > in averages (pressure) makes me confused. I have read from literature
> that
> > the equillibriation of polymers is comparitively diffcult.
>
> Fluctuations in the average pressure do not necessarily mean there's
> a equilibration problem, depending on the time it takes for the
> average pressure to converge for your system. Simply a matter of
> statistics.
>
> Since the pressure is always fluctuating enormously and converging
> slowly compared to other observables, I don't think that (only)
> checking the average pressure is a good way of determining whether a
> system is equilibrated or not. Of course, a drift in the average
> pressure could mean that your system is not equilibrated. But in that
> case you'd probably also see a significantly large drift in other
> observables, especially the potential energy and partial potential
> energy terms in your case.
>
> Cheers,
> Jeroen





Hi jeroen

Thank you for the reply. I forgot to mention the avergae potential , kinetic
and total energy for the simulation runs are as follows

###############################################################################################################
5ns NPT simulation


Last frame read 10000 time 5000.000


Statistics over 5000001 steps [ 0.0000 thru 5000.0000 ps ], 1 data sets

Energy                      Average       RMSD     Fluct.
Drift             Tot-Drift
------------------------------
-------------------------------------------------
Total-Energy              4331.96    355.493    355.355   0.00685523
34.2761

Potential energy       -9401.33     250.37     250.31    0.00378263
18.9132

Kinetic energy           13733.3     235.6        235.558    0.0030726
15.363

Pressure                   10.4371    724.216    724.216    -8.51094e-05  -
0.425347

Temperature              450           7.71993   7.71856     0.0010068
34.2761

Density                     916.781     6.60382    6.60252    9.09303e-05
0.454652

##################################################################################################

5ns NVT simulation


Last frame read 10000 time 5000.000


Statistics over 5000001 steps [ 0.0000 thru 5000.0000 ps ], 1 data sets

Energy                      Average       RMSD     Fluct.
Drift             Tot-Drift
-------------------------------------------------------------------------------
Total-Energy              4335.29    352.244    351.929   0.0103137
51.5683

Potential energy       -9397.99     244.804   244.693   0.00509701
25.4881

Kinetic energy           13733.3     236.317   236.197    0.00521605
26.0803

Pressure                   18.06        802.822    802.807  -0.00349252  -
17.4726

Temperature              450           7.74343    7.7395     0.000170915
0.854575

Density                     927.262 (g_density -f traj.xtc -s ppo.tpr -sl 1)


#######################################################################################################

10ns NVT simulation


Energy                      Average       RMSD     Fluct.
Drift             Tot-Drift
-------------------------------------------------------------------------------
Total-Energy              4339.31    350.971    350.812   -0.00732073   -
36.6037

Potential energy       -9393.97     244.091   243.963   -0.0054728    -
27.3624

Kinetic energy           13733.3     236.317   236.197    0.00521605
26.0803

Pressure                    28.5734     801.629  801.56    0.00729491
39.4746

Temperature              450           7.73025    7.72976   -6.05616e-05   -
0.302808

Density                     927.263 (g_density -f traj.xtc -s ppo.tpr -sl 1)


########################################################################################################


The above mentioned are the averages for the system in the cubical box where
the coordinates have vallues greater than the box length.
One of my question was left unanswered regarding the calculation of density.


1.Can i use (g_density -f traj.xtc -s ppo.tpr -sl 1) to calculate the
density of the system in NVT simulation? are the options correct to
calculate the density?

2. When I view the trajectories (ngmx -f traj.xtc -s top.tpr) of my run i
always found my polymer chains hanging out of the box.Is it the periodic
boundary condition that i observe in veiwing the trajectory? (as i mentioned
before some of my cordinates are greater than box length). is that can be
the reason for the same?

3. How can i keep all my molecules inside the box during the simulation run?






Thanking you in advance
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