[gmx-users] CSDs

David van der Spoel spoel at xray.bmc.uu.se
Tue Nov 6 21:46:35 CET 2007


jahanshah ashkani wrote:
> 
> Thank you for the reply. I used that and I defined CSD residue in 
> ffG43a1.rtp file. GROMACS made a .top file but I have got the following 
> error when I run grompp:
> 
read chapter 5 of the manual and check the wiki. all info is there.

> processing topology...
> Generated 279 of the 1225 non-bonded parameter combinations
> ERROR 0 [file "wt_A.itp", line 11115]:
>   No default Proper Dih. types
> ERROR 0 [file "wt_A.itp", line 11119]:
>   No default Proper Dih. types
> Excluding 3 bonded neighbours for Protein_A           1
> Excluding 3 bonded neighbours for Protein_B           1
> NOTE:
>   System has non-zero total charge: -1.386400e+01
> 
> processing coordinates...
> double-checking input for internal consistency...
> renumbering atomtypes...
> converting bonded parameters...
> #   G96BONDS:   4516
> #  G96ANGLES:   6596
> #      PDIHS:   2363
> #      IDIHS:   2292
> #       LJ14:   7108
> 
> -------------------------------------------------------
> Program grompp, VERSION 3.3.2
> Source code file: grompp.c, line: 1182
> 
> Fatal error:
> There were 2 errors in input file(s)
> -------------------------------------------------------
> 
> I would be glad if you help me in this case.
> Thank you very much.
>  
> Jahanshah Ashkani,
> PhD student of Biotechnology & Genetics,
> University of the Western Cape,
> Biotechnology Department,
> Private Bag X17,
> 7735 Bellville,
> Cape Town,
> South Africa
> jashkani at mail.biotech.uwc.ac.za
> 
> 
> ----- Original Message ----
> From: David van der Spoel <spoel at xray.bmc.uu.se>
> To: Discussion list for GROMACS users <gmx-users at gromacs.org>
> Sent: Tuesday, November 6, 2007 10:28:17 PM
> Subject: Re: [gmx-users] CSDs
> 
> jahanshah ashkani wrote:
>  >
>  >
>  >
>  >    3-sulfinoalanine
> 
> please try prodrg, but you probably have to compute charges in some oe
> another too. read literature!
>  >
>  > 
>  > Jahanshah Ashkani,
>  > PhD student of Biotechnology & Genetics,
>  > University of the Western Cape,
>  > Biotechnology Department,
>  > Private Bag X17,
>  > 7735 Bellville,
>  > Cape Town,
>  > South Africa
>  > jashkani at mail.biotech.uwc.ac.za <mailto:jashkani at mail.biotech.uwc.ac.za>
>  >
>  >
>  > ----- Original Message ----
>  > From: David van der Spoel <spoel at xray.bmc.uu.se 
> <mailto:spoel at xray.bmc.uu.se>>
>  > To: Discussion list for GROMACS users <gmx-users at gromacs.org 
> <mailto:gmx-users at gromacs.org>>
>  > Sent: Thursday, November 1, 2007 8:38:59 PM
>  > Subject: Re: [gmx-users] CSDs
>  >
>  > syawash Rezaei wrote:
>  >  > Hi there,
>  >  >  I would be glad if you let me know how can I use a
>  >  > pdb file with CSDs in GROMACS.
>  >
>  > and what might that be?
>  >
>  >  >  Thank you very much.
>  >  > --- gmx-users-request at gromacs.org 
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>  >  >>    1. "Specified frame doesn't exist or file not
>  >  >> seekable"    when
>  >  >>      using .xtc (Qiao Baofu)
>  >  >>    2. Re: "Specified frame doesn't exist or file not
>  >  >> seekable"    when
>  >  >>      using .xtc (David van der Spoel)
>  >  >>    3. Re: "Specified frame doesn't exist or file not
>  >  >> seekable"    when
>  >  >>      using .xtc (Qiao Baofu)
>  >  >>    4. Re: "Specified frame doesn't exist or file not
>  >  >> seekable"    when
>  >  >>      using .xtc (David van der Spoel)
>  >  >>    5. *.gro output file being written to inverse
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>  >  >>    6. mdrun 3.3.2 crash - gmx_sumi called in serial
>  >  >> run with    RF
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>  >  > ----------------------------------------------------------------------
>  >  >> Message: 1
>  >  >> Date: Thu, 1 Nov 2007 16:17:32 +0100
>  >  >> From: "Qiao Baofu" <qiaobf at gmail.com <mailto:qiaobf at gmail.com> 
> <mailto:qiaobf at gmail.com <mailto:qiaobf at gmail.com>>>
>  >  >> Subject: [gmx-users] "Specified frame doesn't exist
>  >  >> or file not
>  >  >>    seekable"    when using .xtc
>  >  >> To: gmx-users at gromacs.org <mailto:gmx-users at gromacs.org> 
> <mailto:gmx-users at gromacs.org <mailto:gmx-users at gromacs.org>>
>  >  >> Message-ID:
>  >  >>
>  >  >>
>  >  > <6a91f07b0711010817wb3e9454j7145001c33e8449d at mail.gmail.com 
> <mailto:6a91f07b0711010817wb3e9454j7145001c33e8449d at mail.gmail.com>
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> <mailto:6a91f07b0711010817wb3e9454j7145001c33e8449d at mail.gmail.com>>>
>  >  >> Content-Type: text/plain; charset="iso-8859-1"
>  >  >>
>  >  >> Hi, all
>  >  >>
>  >  >> When I using the .xtc file to analyse, I always meet
>  >  >> the following error
>  >  >> information
>  >  >>  Specified frame doesn't exist or file not seekable
>  >  >> While it works when using the .trr file. In
>  >  >> analyzing, only the coordinate
>  >  >> is used. Therefore, I want to use .xtc file. In this
>  >  >> way, it runs a little
>  >  >> faster. However, the above proble exits.  What's
>  >  >> wrong with the .xtc file?
>  >  >> and how to solve it?
>  >  >>
>  >  >> Thanks!
>  >  >>
>  >  >> --
>  >  >> Sincerely yours,
>  >  >> Dr. Baofu Qiao
>  >  >> -------------- next part --------------
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>  >  >> ------------------------------
>  >  >>
>  >  >> Message: 2
>  >  >> Date: Thu, 01 Nov 2007 16:19:29 +0100
>  >  >> From: David van der Spoel <spoel at xray.bmc.uu.se 
> <mailto:spoel at xray.bmc.uu.se>
>  > <mailto:spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>>>
>  >  >> Subject: Re: [gmx-users] "Specified frame doesn't
>  >  >> exist or file not
>  >  >>    seekable"    when using .xtc
>  >  >> To: Discussion list for GROMACS users
>  >  >> <gmx-users at gromacs.org <mailto:gmx-users at gromacs.org> 
> <mailto:gmx-users at gromacs.org <mailto:gmx-users at gromacs.org>>>
>  >  >> Message-ID: <4729EE81.5010704 at xray.bmc.uu.se 
> <mailto:4729EE81.5010704 at xray.bmc.uu.se>
>  > <mailto:4729EE81.5010704 at xray.bmc.uu.se 
> <mailto:4729EE81.5010704 at xray.bmc.uu.se>>>
>  >  >> Content-Type: text/plain; charset=ISO-8859-1;
>  >  >> format=flowed
>  >  >>
>  >  >> Qiao Baofu wrote:
>  >  >>> Hi, all
>  >  >>>
>  >  >>> When I using the .xtc file to analyse, I always
>  >  >> meet the following error
>  >  >>> information
>  >  >>>  Specified frame doesn't exist or file not
>  >  >> seekable
>  >  >>> While it works when using the .trr file. In
>  >  >> analyzing, only the
>  >  >>> coordinate is used. Therefore, I want to use .xtc
>  >  >> file. In this way, it
>  >  >>> runs a little faster. However, the above proble
>  >  >> exits.  What's wrong
>  >  >>> with the .xtc file? and how to solve it?
>  >  >>>
>  >  >> gmx version please.
>  >  >>> Thanks!
>  >  >>>
>  >  >>> --
>  >  >>> Sincerely yours,
>  >  >>> Dr. Baofu Qiao
>  >  >>>
>  >  >>>
>  >  >>>
>  >  > 
> ------------------------------------------------------------------------
>  >  >>> _______________________________________________
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>  >  >>
>  >  >> --
>  >  >> David van der Spoel, Ph.D.
>  >  >> Molec. Biophys. group, Dept. of Cell & Molec. Biol.,
>  >  >> Uppsala University.
>  >  >> Box 596, 75124 Uppsala, Sweden. Phone:    +46184714205.
>  >  >> Fax: +4618511755.
>  >  >> spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se> 
> <mailto:spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>>   
>  > spoel at gromacs.org <mailto:spoel at gromacs.org> 
> <mailto:spoel at gromacs.org <mailto:spoel at gromacs.org>>
>  >  >> http://folding.bmc.uu.se
>  >  >>
>  >  >>
>  >  >> ------------------------------
>  >  >>
>  >  >> Message: 3
>  >  >> Date: Thu, 1 Nov 2007 16:27:39 +0100
>  >  >> From: "Qiao Baofu" <qiaobf at gmail.com <mailto:qiaobf at gmail.com> 
> <mailto:qiaobf at gmail.com <mailto:qiaobf at gmail.com>>>
>  >  >> Subject: Re: [gmx-users] "Specified frame doesn't
>  >  >> exist or file not
>  >  >>    seekable"    when using .xtc
>  >  >> To: "Discussion list for GROMACS users"
>  >  >> <gmx-users at gromacs.org <mailto:gmx-users at gromacs.org> 
> <mailto:gmx-users at gromacs.org <mailto:gmx-users at gromacs.org>>>
>  >  >> Message-ID:
>  >  >>
>  >  >>
>  >  > <6a91f07b0711010827y4611ed4ej309e0ebf5c67daed at mail.gmail.com 
> <mailto:6a91f07b0711010827y4611ed4ej309e0ebf5c67daed at mail.gmail.com>
>  > <mailto:6a91f07b0711010827y4611ed4ej309e0ebf5c67daed at mail.gmail.com 
> <mailto:6a91f07b0711010827y4611ed4ej309e0ebf5c67daed at mail.gmail.com>>>
>  >  >> Content-Type: text/plain; charset="iso-8859-1"
>  >  >>
>  >  >> Hi,
>  >  >>
>  >  >> I am using Gromacs 3.3.1.  The .xtc file is obtained
>  >  >> from mdrun, as shown in
>  >  >> the following
>  >  >>
>  >  >> ; OUTPUT CONTROL OPTIONS
>  >  >> ; Output frequency for coords (x), velocities (v)
>  >  >> and forces (f)
>  >  >> nstxout                  = 0
>  >  >> nstvout                  = 0
>  >  >> nstfout                  = 0
>  >  >> ; Checkpointing helps you continue after crashes
>  >  >> nstcheckpoint            = 0
>  >  >> ; Output frequency for energies to log file and
>  >  >> energy file
>  >  >> nstlog                  = 1000
>  >  >> nstenergy                = 100
>  >  >> ; Output frequency and precision for xtc file
>  >  >> nstxtcout                = 10
>  >  >> xtc-precision            = 1000
>  >  >>
>  >  >>
>  >  >>
>  >  >>
>  >  >>
>  >  >> 2007/11/1, David van der Spoel
>  >  >> <spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se> 
> <mailto:spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>>>:
>  >  >>> Qiao Baofu wrote:
>  >  >>>> Hi, all
>  >  >>>>
>  >  >>>> When I using the .xtc file to analyse, I always
>  >  >> meet the following error
>  >  >>>> information
>  >  >>>>  Specified frame doesn't exist or file not
>  >  >> seekable
>  >  >>>> While it works when using the .trr file. In
>  >  >> analyzing, only the
>  >  >>>> coordinate is used. Therefore, I want to use
>  >  >> .xtc file. In this way, it
>  >  >>>> runs a little faster. However, the above proble
>  >  >> exits.  What's wrong
>  >  >>>> with the .xtc file? and how to solve it?
>  >  >>>>
>  >  > === message truncated ===
>  >  >
>  >  >
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>  >
>  > --
>  > David van der Spoel, Ph.D.
>  > Molec. Biophys. group, Dept. of Cell & Molec. Biol., Uppsala University.
>  > Box 596, 75124 Uppsala, Sweden. Phone:    +46184714205. Fax: +4618511755.
>  > spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se> 
> <mailto:spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>>    
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> 
> -- 
> David van der Spoel, Ph.D.
> Molec. Biophys. group, Dept. of Cell & Molec. Biol., Uppsala University.
> Box 596, 75124 Uppsala, Sweden. Phone:    +46184714205. Fax: +4618511755.
> spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>    spoel at gromacs.org 
> <mailto:spoel at gromacs.org>  http://folding.bmc.uu.se
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-- 
David van der Spoel, Ph.D.
Molec. Biophys. group, Dept. of Cell & Molec. Biol., Uppsala University.
Box 596, 75124 Uppsala, Sweden. Phone:	+46184714205. Fax: +4618511755.
spoel at xray.bmc.uu.se	spoel at gromacs.org   http://folding.bmc.uu.se



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