[gmx-users] tfe

shahrbanoo karbalaee shahrbanoo at gmail.com
Fri Aug 15 11:33:54 CEST 2008


Dear justin
I try again .but I get this error toputil  .line 1364
not found such molecoule SOL.then would you please check my topol.top
file and advise me.


PEPTIDE A5 OR AUREIN 1.2
;
; Include forcefield parameters
#include "ffoplsaa.itp"

[ moleculetype ]
; Name            nrexcl
Protein_A           3

[ atoms ]
;   nr       type  resnr residue  atom   cgnr     charge       mass
typeB    chargeB      massB
     1   opls_900      1    GLY      N      1       -0.9    14.0067
; qtot -0.9
     2   opls_909      1    GLY     H1      1       0.36      1.008
; qtot -0.54
     3   opls_909      1    GLY     H2      1       0.36      1.008
; qtot -0.18
     4  opls_906B      1    GLY     CA      1       0.06     12.011
; qtot -0.12
     5   opls_140      1    GLY    HA1      1       0.06      1.008
; qtot -0.06
     6   opls_140      1    GLY    HA2      1       0.06      1.008   ; qtot 0
     7   opls_235      1    GLY      C      2        0.5     12.011   ; qtot 0.5
     8   opls_236      1    GLY      O      2       -0.5    15.9994   ; qtot 0
     9   opls_238      2    LEU      N      3       -0.5    14.0067
; qtot -0.5
    10   opls_241      2    LEU      H      3        0.3      1.008
; qtot -0.2
    11  opls_224B      2    LEU     CA      3       0.14     12.011
; qtot -0.06
    12   opls_140      2    LEU     HA      3       0.06      1.008   ; qtot 0
    13   opls_136      2    LEU     CB      4      -0.12     12.011
; qtot -0.12
    14   opls_140      2    LEU    HB1      4       0.06      1.008
; qtot -0.06
    15   opls_140      2    LEU    HB2      4       0.06      1.008   ; qtot 0
    16   opls_137      2    LEU     CG      5      -0.06     12.011
; qtot -0.06
    17   opls_140      2    LEU     HG      5       0.06      1.008   ; qtot 0
    18   opls_135      2    LEU    CD1      6      -0.18     12.011
; qtot -0.18
    19   opls_140      2    LEU   HD11      6       0.06      1.008
; qtot -0.12
    20   opls_140      2    LEU   HD12      6       0.06      1.008
; qtot -0.06
    21   opls_140      2    LEU   HD13      6       0.06      1.008   ; qtot 0
    22   opls_135      2    LEU    CD2      7      -0.18     12.011
; qtot -0.18
    23   opls_140      2    LEU   HD21      7       0.06      1.008
; qtot -0.12
    24   opls_140      2    LEU   HD22      7       0.06      1.008
; qtot -0.06
    25   opls_140      2    LEU   HD23      7       0.06      1.008   ; qtot 0
    26   opls_235      2    LEU      C      8        0.5     12.011   ; qtot 0.5
    27   opls_236      2    LEU      O      8       -0.5    15.9994   ; qtot 0
    28   opls_238      3    PHE      N      9       -0.5    14.0067
; qtot -0.5
    29   opls_241      3    PHE      H      9        0.3      1.008
; qtot -0.2
    30  opls_224B      3    PHE     CA      9       0.14     12.011
; qtot -0.06
    31   opls_140      3    PHE     HA      9       0.06      1.008   ; qtot 0
    32   opls_149      3    PHE     CB     10     -0.005     12.011
; qtot -0.005
    33   opls_140      3    PHE    HB1     10       0.06      1.008
; qtot 0.055
    34   opls_140      3    PHE    HB2     10       0.06      1.008
; qtot 0.115
    35   opls_145      3    PHE     CG     10     -0.115     12.011   ; qtot 0
    36   opls_145      3    PHE    CD1

  219     opls_240     14    NH2     H2     73       0.38      1.008   ; qtot 0

[ bonds ]



[ pairs ]
[ angles ]


[ dihedrals ]
;  ai    aj    ak    al funct            c0            c1
c2            c3
    4     9     7     8     1    improper_O_C_X_Y
    7    11     9    10     1    improper_Z_N_X_Y
   11    28    26    27     1    improper_O_C_X_Y
   26    30    28    29     1    improper_Z_N_X_Y
   30    48    46    47     1    improper_O_C_X_Y
   32    35    38    36     1    improper_Z_CA_X_Y
   35    40    36    37     1    improper_Z_CA_X_Y
   35    42    38    39     1    improper_Z_CA_X_Y
   36    44    40    41     1    improper_Z_CA_X_Y
   38    44    42    43     1    improper_Z_CA_X_Y
   40    42    44    45     1    improper_Z_CA_X_Y
   46    50    48    49     1    improper_Z_N_X_Y
   50    60    58    59     1    improper_O_C_X_Y
   52    56    55    57     1    improper_O_C_X_Y
   58    62    60    61     1    improper_Z_N_X_Y
   62    79    77    78     1    improper_O_C_X_Y
   77    81    79    80     1    improper_Z_N_X_Y
   81    98    96    97     1    improper_O_C_X_Y
   96   100    98    99     1    improper_Z_N_X_Y
  100   120   118   119     1    improper_O_C_X_Y
  118   122   120   121     1    improper_Z_N_X_Y
  122   142   140   141     1    improper_O_C_X_Y
  140   144   142   143     1    improper_Z_N_X_Y
  144   161   159   160     1    improper_O_C_X_Y
  159   163   161   162     1    improper_Z_N_X_Y
  163   171   169   170     1    improper_O_C_X_Y
  169   173   171   172     1    improper_Z_N_X_Y
  173   186   184   185     1    improper_O_C_X_Y
  178   182   181   183     1    improper_O_C_X_Y
  184   188   186   187     1    improper_Z_N_X_Y
  188   197   195   196     1    improper_O_C_X_Y
  195   199   197   198     1    improper_Z_N_X_Y
  199   217   215   216     1    improper_O_C_X_Y
  201   204   207   205     1    improper_Z_CA_X_Y
  204   209   205   206     1    improper_Z_CA_X_Y
  204   211   207   208     1    improper_Z_CA_X_Y
  205   213   209   210     1    improper_Z_CA_X_Y
  207   213   211   212     1    improper_Z_CA_X_Y
  209   211   213   214     1    improper_Z_CA_X_Y
  215   218   217   219     1    improper_Z_N_X_Y

; Include Position restraint file
#ifdef POSRES
#include "posre.itp"
; Include water topology
#include "spce.itp"
Include tfe topology
#include "tfe.itp"

#endif

#ifdef POSRES_WATER
; Position restraint for each water oxygen
[ position_restraints ]
;  i funct       fcx        fcy        fcz
   1    1       1000       1000       1000

#endif

; Include generic topology for ions
#include "ions.itp"

[ system ]
; Name
PEPTIDE A5 OR AUREIN 1.2 in 30%tfe  water

[ molecules ]
; Compound        #mols
Protein_A           1
SOL               941
tfe               301

---------------------------------------------------
 and the file tfe.gro is:

Glycine aRginine prOline Methionine Alanine Cystine Serine
    9
    1TFE    CC'    1  -0.098  -0.156   0.352
    1TFE    FD'    2  -0.010  -0.129   0.451
    1TFE    FE'    3  -0.143  -0.282   0.370
    1TFE    FF'    4  -0.203  -0.072   0.366
    1TFE    CB'    5  -0.033  -0.140   0.217
    1TFE   HB'1    6  -0.106  -0.161   0.139
    1TFE   HB'2    7   0.051  -0.209   0.209
    1TFE    O2'    8   0.014  -0.006   0.204
    1TFE    HO2    9   0.055   0.005   0.118
   0.00000   0.00000   0.00000


thanks and best regards
-- 
sh-karbalaee



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