[gmx-users] problem regarding .top file generated by genbox

sudheer babu sudheer.pbm07 at gmail.com
Mon Feb 18 15:58:22 CET 2008


HI gmxusers,
i am using gromacs version 3.3.1
i am new to gromocs, i was taken protein.top file (which is generated by
pdb2gmx). In this file i have included ff.itp , popc.itp and lipid.itp but
protein atom types already there (generated by pdb2gmx). This .top file used
for inserting of protein into mebranes by using genbox. It was run without
problem, given .gro which contain both protein and popc and .top file . when
i have used this .top and .gro for energy minimisation it's showing that,
Fatal error:
Atom types NR, HC , CR1 not found
these type of atoms not there in .gro file but present in .top file .
Iam pasting the aminoacids which contain these type of atoms

17          N      3   HISH      N      7      -0.28    14.0067   ; qtot
0.72
    18          H      3   HISH      H      7       0.28      1.008   ; qtot
1
    19        CH1      3   HISH     CA      8          0     13.019   ; qtot
1
    20        CH2      3   HISH     CB      8          0     14.027   ; qtot
1
    21          C      3   HISH     CG      9      -0.05     12.011   ; qtot
0.95
    22         NR      3   HISH    ND1      9       0.38    14.0067   ; qtot
1.33
    23          H      3   HISH    HD1      9        0.3      1.008   ; qtot
1.63
    24        CR1      3   HISH    CD2      9          0     13.019   ; qtot
1.63
    25        CR1      3   HISH    CE1      9      -0.24     13.019   ; qtot
1.39
    26         NR      3   HISH    NE2      9       0.31    14.0067   ; qtot
1.7
    27          H      3   HISH    HE2      9        0.3      1.008   ; qtot
2
    28          C      3   HISH      C     10       0.38     12.011   ; qtot
2.38
    29          O      3   HISH      O     10      -0.38    15.9994   ; qtot
2
    30          N      4   HISH      N     11      -0.28    14.0067   ; qtot
1.72
    31          H      4   HISH      H     11       0.28      1.008   ; qtot
2
    32        CH1      4   HISH     CA     12          0     13.019   ; qtot
2
    33        CH2      4   HISH     CB     12          0     14.027   ; qtot
2
    34          C      4   HISH     CG     13      -0.05     12.011   ; qtot
1.95
    35         NR      4   HISH    ND1     13       0.38    14.0067   ; qtot
2.33
    36          H      4   HISH    HD1     13        0.3      1.008   ; qtot
2.63
    37        CR1      4   HISH    CD2     13          0     13.019   ; qtot
2.63
    38        CR1      4   HISH    CE1     13      -0.24     13.019   ; qtot
2.39
    39         NR      4   HISH    NE2     13       0.31    14.0067   ; qtot
2.7
    40          H      4   HISH    HE2     13        0.3      1.008   ; qtot
3
    41          C      4   HISH      C     14       0.38     12.011   ; qtot
3.38
    42          O      4   HISH      O     14      -0.38    15.9994   ; qtot
3


  308          N     36    PHE      N    133      -0.28    14.0067   ; qtot
2.72
   309          H     36    PHE      H    133       0.28      1.008   ; qtot
3
   310        CH1     36    PHE     CA    134          0     13.019   ; qtot
3
   311        CH2     36    PHE     CB    134          0     14.027   ; qtot
3
   312          C     36    PHE     CG    134          0     12.011   ; qtot
3
   313          C     36    PHE    CD1    135       -0.1     12.011   ; qtot
2.9
   314         HC     36    PHE    HD1    135        0.1      1.008   ; qtot
3
   315          C     36    PHE    CD2    136       -0.1     12.011   ; qtot
2.9
   316         HC     36    PHE    HD2    136        0.1      1.008   ; qtot
3
   317          C     36    PHE    CE1    137       -0.1     12.011   ; qtot
2.9
   318         HC     36    PHE    HE1    137        0.1      1.008   ; qtot
3
   319          C     36    PHE    CE2    138       -0.1     12.011   ; qtot
2.9
   320         HC     36    PHE    HE2    138        0.1      1.008   ; qtot
3
   321          C     36    PHE     CZ    139       -0.1     12.011   ; qtot
2.9
   322         HC     36    PHE     HZ    139        0.1      1.008   ; qtot
3
   323          C     36    PHE      C    140       0.38     12.011   ; qtot
3.38
   324          O     36    PHE      O    140      -0.38    15.9994   ; qtot
3

have i given wrong command
pls help me,
thanks in advance.
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