[gmx-users] Error: number of atoms do not match

h a y600100 at gmail.com
Wed Jul 9 14:02:58 CEST 2008


Dear Users,

I used prodrg( gomos96.1) to generate topology and .gro files of polystyrene
( of 10 monomers ). the read me file is as shown
*****
DRGFIN.PDB  : the final PDB file with all hydrogens added
DRGPOH.PDB  : the final PDB file with only polar hydrogens added
DRGNOH.PDB  : the final PDB file without any hydrogens added

DRGFIN.MOL  : the final Molfile with all hydrogens added
DRGPOH.MOL  : the final Molfile with only polar hydrogens added
DRGNOH.MOL  : the final Molfile without any hydrogens added

DRGFIN.GRO  : final coordinates in GROMOS87 format (all hydrogens)
DRGPOH.GRO  : final coordinates in GROMOS87 format (polar hydrogens only)

DRGCNS.PAR  : CNS parameter file
DRGCNS.TOP  : CNS topology file

DRGMAC.LIB  : REFMAC5 library

DRGSHX.TOP  : SHELX topology

DRGTRS.O    : O <9.0 torsion database (read in with: read DRGTRS.O in O)
DRGFNOH.O6  : O <9.0 refi dictionary
DRGNOH.O    : O 9.x dictionary

DRGGMX.ITP  : GROMACS .itp file

DRGWIF.TOP  : WHAT IF topology

DRGFIN.MOL2 : SYBYL2 file with all hydrogens
DRGPOH.MOL2 : SYBYL2 file with only polar hydrogens

DRGAD2.PDBQ : AutoDock 2.4 PDBQ file
DRGAD3.PDBQ : AutoDock 3.0 PDBQ file

DRGHEX.TOP  : HEX topology
*****************

Now I'm actually studying protein polymer_surface interactions, using g43a1
forcefield . Can anybody explain whether should I use polymer model with
only polar hydrogens or polymer model with all hydrogens ? What is the
significance of these hydrogens.
In the .itp file(shown below) there are 130 atoms but total number of atoms
in only polar hydrogens model is 80 same with no hydrogens model. Including
all hydrogens I have 162 atoms. So what does this 130 correspond to ?? (
actually I'm facing fatal error because of this consistency in number of
atoms while doing EM )
*******
[ atoms ]
;   nr      type  resnr resid  atom  cgnr   charge     mass
     1       CR1     1  STY     CAB     1   -0.006  12.0110
     2        HC     1  STY     HAB     1    0.006   1.0080
     3       CR1     1  STY     CAC     2   -0.016  12.0110
     4        HC     1  STY     HAC     2    0.002   1.0080
     5       CR1     1  STY     CAD     2   -0.017  12.0110
     6        HC     1  STY     HAD     2    0.002   1.0080
     7       CR1     1  STY     CAE     2   -0.017  12.0110
     8        HC     1  STY     HAE     2    0.002   1.0080
     9       CR1     1  STY     CAF     2   -0.017  12.0110
    10        HC     1  STY     HAF     2    0.002   1.0080
    11         C     1  STY     CAA     2   -0.025  12.0110
    12       CH1     1  STY     CAG     2    0.067  13.0190
    13       CH3     1  STY     CAH     2    0.017  15.0350
    14       CH2     1  STY     CAP     3    0.000  14.0270
    15       CH1     1  STY     CAO     4    0.060  13.0190
    16         C     1  STY     CAI     4   -0.027  12.0110
    17       CR1     1  STY     CAJ     4   -0.018  12.0110
    18        HC     1  STY     HAK     4    0.001   1.0080
    19       CR1     1  STY     CAK     4   -0.018  12.0110
    20        HC     1  STY     HAL     4    0.002   1.0080
    21       CR1     1  STY     CAL     5   -0.016  12.0110
    22        HC     1  STY     HAM     5    0.003   1.0080
    23       CR1     1  STY     CAM     5   -0.016  12.0110
    24        HC     1  STY     HAN     5    0.003   1.0080
    25       CR1     1  STY     CAN     5   -0.016  12.0110
    26        HC     1  STY     HAO     5    0.003   1.0080
    27       CH2     1  STY     CAX     5    0.039  14.0270
    28       CH1     1  STY     CAW     6    0.060  13.0190
    29         C     1  STY     CAQ     6   -0.027  12.0110
    30       CR1     1  STY     CAR     6   -0.018  12.0110
    31        HC     1  STY     HAS     6    0.001   1.0080
    32       CR1     1  STY     CAS     6   -0.018  12.0110
    33        HC     1  STY     HAT     6    0.002   1.0080
    34       CR1     1  STY     CAT     7   -0.016  12.0110
    35        HC     1  STY     HAU     7    0.003   1.0080
    36       CR1     1  STY     CAU     7   -0.016  12.0110
    37        HC     1  STY     HAV     7    0.003   1.0080
    38       CR1     1  STY     CAV     7   -0.016  12.0110
    39        HC     1  STY     HAW     7    0.003   1.0080
    40       CH2     1  STY     CBF     7    0.039  14.0270
    41       CH1     1  STY     CBE     8    0.060  13.0190
    42         C     1  STY     CAY     8   -0.027  12.0110
    43       CR1     1  STY     CAZ     8   -0.018  12.0110
    44        HC     1  STY     HA0     8    0.001   1.0080
    45       CR1     1  STY     CBA     8   -0.018  12.0110
    46        HC     1  STY     HBA     8    0.002   1.0080
    47       CR1     1  STY     CBB     9   -0.016  12.0110
    48        HC     1  STY     HBB     9    0.003   1.0080
    49       CR1     1  STY     CBC     9   -0.016  12.0110
    50        HC     1  STY     HBC     9    0.003   1.0080
    51       CR1     1  STY     CBD     9   -0.016  12.0110
    52        HC     1  STY     HBD     9    0.003   1.0080
    53       CH2     1  STY     CBN     9    0.039  14.0270
    54       CH1     1  STY     CBM    10    0.060  13.0190
    55         C     1  STY     CBG    10   -0.027  12.0110
    56       CR1     1  STY     CBH    10   -0.018  12.0110
    57        HC     1  STY     HBH    10    0.001   1.0080
    58       CR1     1  STY     CBI    10   -0.018  12.0110
    59        HC     1  STY     HBI    10    0.002   1.0080
    60       CR1     1  STY     CBJ    11   -0.016  12.0110
    61        HC     1  STY     HBJ    11    0.003   1.0080
    62       CR1     1  STY     CBK    11   -0.016  12.0110
    63        HC     1  STY     HBK    11    0.003   1.0080
    64       CR1     1  STY     CBL    11   -0.016  12.0110
    65        HC     1  STY     HBL    11    0.003   1.0080
    66       CH2     1  STY     CBV    11    0.039  14.0270
    67       CH1     1  STY     CBU    12    0.060  13.0190
    68         C     1  STY     CBO    12   -0.027  12.0110
    69       CR1     1  STY     CBP    12   -0.018  12.0110
    70        HC     1  STY     HBP    12    0.001   1.0080
    71       CR1     1  STY     CBQ    12   -0.018  12.0110
    72        HC     1  STY     HBQ    12    0.002   1.0080
    73       CR1     1  STY     CBR    13   -0.016  12.0110
    74        HC     1  STY     HBR    13    0.003   1.0080
    75       CR1     1  STY     CBS    13   -0.016  12.0110
    76        HC     1  STY     HBS    13    0.003   1.0080
    77       CR1     1  STY     CBT    13   -0.016  12.0110
    78        HC     1  STY     HBT    13    0.003   1.0080
    79       CH2     1  STY     CCD    13    0.039  14.0270
    80       CH1     1  STY     CCC    14    0.060  13.0190
    81         C     1  STY     CBW    14   -0.027  12.0110
    82       CR1     1  STY     CBX    14   -0.018  12.0110
    83        HC     1  STY     HBX    14    0.001   1.0080
    84       CR1     1  STY     CBY    14   -0.018  12.0110
    85        HC     1  STY     HBY    14    0.002   1.0080
    86       CR1     1  STY     CBZ    15   -0.016  12.0110
    87        HC     1  STY     HBZ    15    0.003   1.0080
    88       CR1     1  STY     CCA    15   -0.016  12.0110
    89        HC     1  STY     HCA    15    0.003   1.0080
    90       CR1     1  STY     CCB    15   -0.016  12.0110
    91        HC     1  STY     HCB    15    0.003   1.0080
    92       CH2     1  STY     CCL    15    0.039  14.0270
    93       CH1     1  STY     CCK    16    0.060  13.0190
    94         C     1  STY     CCE    16   -0.027  12.0110
    95       CR1     1  STY     CCF    16   -0.018  12.0110
    96        HC     1  STY     HCF    16    0.001   1.0080
    97       CR1     1  STY     CCG    16   -0.018  12.0110
    98        HC     1  STY     HCG    16    0.002   1.0080
    99       CR1     1  STY     CCH    17   -0.016  12.0110
   100        HC     1  STY     HCH    17    0.003   1.0080
   101       CR1     1  STY     CCI    17   -0.016  12.0110
   102        HC     1  STY     HCI    17    0.003   1.0080
   103       CR1     1  STY     CCJ    17   -0.016  12.0110
   104        HC     1  STY     HCJ    17    0.003   1.0080
   105       CH2     1  STY     CCT    17    0.039  14.0270
   106       CH1     1  STY     CCS    18    0.060  13.0190
   107         C     1  STY     CCM    18   -0.027  12.0110
   108       CR1     1  STY     CCN    18   -0.018  12.0110
   109        HC     1  STY     HCN    18    0.001   1.0080
   110       CR1     1  STY     CCO    18   -0.018  12.0110
   111        HC     1  STY     HCO    18    0.002   1.0080
   112       CR1     1  STY     CCP    19   -0.016  12.0110
   113        HC     1  STY     HCP    19    0.003   1.0080
   114       CR1     1  STY     CCQ    19   -0.016  12.0110
   115        HC     1  STY     HCQ    19    0.003   1.0080
   116       CR1     1  STY     CCR    19   -0.016  12.0110
   117        HC     1  STY     HCR    19    0.003   1.0080
   118       CH2     1  STY     CDB    19    0.039  14.0270
   119       CH2     1  STY     CDA    20    0.066  14.0270
   120         C     1  STY     CCU    20   -0.019  12.0110
   121       CR1     1  STY     CCV    20   -0.012  12.0110
   122        HC     1  STY     HCV    20    0.003   1.0080
   123       CR1     1  STY     CCW    20   -0.013  12.0110
   124        HC     1  STY     HCW    20    0.004   1.0080
   125       CR1     1  STY     CCX    20   -0.013  12.0110
   126        HC     1  STY     HCX    20    0.004   1.0080
   127       CR1     1  STY     CCY    20   -0.013  12.0110
   128        HC     1  STY     HCY    20    0.003   1.0080
   129       CR1     1  STY     CCZ    20   -0.013  12.0110
   130        HC     1  STY     HCZ    20    0.003   1.0080

[ bonds ]
; ai  aj  fu    c0, c1, ...
   1   2   2    0.109  12300000.0    0.109  12300000.0 ;   CAB  HAB
   1   3   2    0.139  10800000.0    0.139  10800000.0 ;   CAB  CAC
.
.
.
.
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************

Thank you so much for your help :)

Sincerely,
Harshith, Under Graduate,
IITK
http://home.iitk.ac.in/~harshith
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