[gmx-users] berger-oplsaa combination for DPC micelle

chris.neale at utoronto.ca chris.neale at utoronto.ca
Thu Jun 5 19:25:44 CEST 2008


Hi Soo Mei,

I think that the error messages that you post are pretty descriptive.  
You can definitely use them to figure out what you are missing. I am  
not currently distributing my dpc.itp file beyond the snippit and  
instructions that I posted earlier.

Chris.

> I'm sorry, the line references are for the dpc_double_pairlist.itp file
> I have posted.  I should have made that clearer.
>
> Lines 68-86 are the 4th line of [ bonds ] till the last line of [ bonds]
> .  Lines 138 and 147 are the 8th line and 17th line of [ angles ].
> Lines 161-167 are the first 7 lines of [ dihedrals ].
>
> For completeness I also attach here the ffoplsaanb.itp and
> ffoplsaabon.itp modified according to the half-epsilon double-pairlist
> method.  These forcefield .itp files are in my working directory and are
> read in in full during my failed grompp for minimization, when I checked
> the output .top of this failed grompp.   I had called them using
> #include "ffoplsaa.itp" , the default ffoplsaa.itp not in my working
> directory. grompp command was    grompp -v -f min.mdp -c
> enlarged_merged_solvated_internalwatersremoved.pdb -p berger_opls.top
> -po test_min1out.mdp -pp test_min1out.top -o min1.tpr
>
> Thank you in advance for your advice.  I am completely stuck at this step.
>
> Cheers,
> Soo Mei
>
> -------- Original Message --------
> Subject: 	Re: berger-oplsaa combination for DPC micelle
> Date: 	Thu, 05 Jun 2008 02:42:58 +0800
> From: 	Low Soo Mei <lowsm at bii.a-star.edu.sg>
> Organization: 	BII
> To: 	gmx-users at gromacs.org
> References: 	<20080604160204.C2FA92435F at xray.bmc.uu.se>
>
>
>
> Hi,
>
> Thank you Chris for the helpful answer!  I have modified the atomtype
> names to follow dmpc.itp, which is more familiar to me, but those names
> are also the same as popc.itp.  There is probably something wrong with
> the way I did it because although the "atomtype not found" errors have
> stopped, I am getting "no default bond types (lines 68-86) / angle types
> (lines 138, 147) / proper dihedral types (lines 161-167)".  My modified
> dpc_double_pairlist.itp file is attached below.
>
>> Message: 7
>> Date: Wed, 04 Jun 2008 12:01:33 -0400
>> From: chris.neale at utoronto.ca
>> Subject: [gmx-users] berger-oplsaa combination for DPC micelle
>> To: gmx-users at gromacs.org
>> Message-ID: <20080604120133.htonx5kcw8gg8c48 at webmail.utoronto.ca>
>> Content-Type: text/plain;	charset=ISO-8859-1;	DelSp="Yes";
>> 	format="flowed"
>>
>> In general, you should always post your relevant files with your
>> question. It makes it easier for us to answer you. That said, I happen
>> to have already used DPC and here is what I have done. I made a new
>> dpc_rename.itp and it is quite different, but should simulate the
>> exact same. Here is the beginning of the atoms section
>>
>> ;Chris Neale modified atomtype names here to follow popc.itp
>> ; Atoms double checked vs. popc.itp by CN Oct5 2006
>> [ atoms ]
>> ;   nr    type   resnr  residu    atom    cgnr        charge          mass
>>       1     LC3       1    DPC      C1       1         0.4       ;   
>> qtot: 0.248
>>       2     LC3       1    DPC      C2       1         0.4       ;   
>> qtot: 0.496
>>       3     LC3       1    DPC      C3       1         0.4       ;   
>> qtot: 0.744
>>       4     LNL       1    DPC      N4       1        -0.5       ;   
>> qtot: 0.752
>>       5     LH2       1    DPC      C5       1         0.3       ; qtot: 1
>>
>> and then you need to make modifications to the other sections so that
>> the parameters are found appropriately.
>>
>> Chris.
>>
>> -- original message --
>>
>> Hi Gromacs users,
>>
>> I am trying to simulate a protein with a micelle, and have used dpc.itp
>> from Dr. Tieleman's site.  I have also introduced parts of lipid.itp
>> into ffoplsaanb.itp and ffoplsaabon.itp in accordance with Chris Neale's
>> half-epsilon double-pairlist method.  But when I issue a grompp command
>> for minimization, grompp gives a fatal error that the "Bonded/nonbonded
>> atom type 'CH3' not found!".  This atom type CH3 is present in dpc.itp.
>> I am now wondering if I can match this to some existing atom in the
>> oplsaa and how I might go about choosing which atom.
>>
>> I would be grateful for your advice, and am happy to attach files if needed.
>>
>> Cheers,
>> Soo Mei
>>
>>
>
> dpc_double_pairlist.itp:
>
> ; topology for a dodecylphosphocholine lipid from Tieleman's site.
> ; modified to follow the other pc.itp's on Tieleman's site, as suggested
> by Chris Neale on gmx-users list on 4th June 2008, when I had "atomtype
> not found" problems
> [ bondtypes ]
>  ; i    j func        b0          kb
>  LC3  LNL    1   0.14700       376560. ; formerly CH3 and NL.
> ;  CH1  OS    1   0.14300       251040. ; no CH1 in DPC.
> ;  OS    C    1   0.13600       251040. ; no C in DPC.  C is the
> ester-linkage carbon that joins the glycerol backbone to the long lipid
> chains in the pc.itp's.
>
> [ angletypes ]
>  ; i    j    k func       th0         cth
>  LC3   LNL  LC3    1   109.500      460.00 ; formerly CH3 NL CH3
>  LC3   LNL  LH2    1   109.500      460.00 ; formerly CH3 NL CH2
> ;  CH2  CH1   OS    1   109.500      460.00 ; no CH1 in DPC.
>  LNL   LH2  LC2    1   109.500      460.00 ; formerly NL CH2 CH2
>    LP   LOS  LC2    1   120.000      397.00 ; formerly P OS CH2.  Can't
> decide if it's 8 7 6 or 8 11 12. Will put for both. This is 8 7 6
>    LP   LOS  LP2    1   120.000      397.00 ; and this is 8 11 12.
>   LOS    LP   LOM    1   109.600      397.00 ; formerly OS P OM
>   LOS    LP   LOS    1   103.000      397.00 ; formerly OS P OS
>   LOM    LP   LOM    1   120.000      585.00 ; formerly OM P OM
> ;   OS  CH2  CH1    1   111.000      460.00 ; no CH1 in DPC.
> ;  CH2  CH2  CH1    1   111.000      460.00 ; no CH1 in DPC.
>  LP2  LP2  LP2    1   111.000      460.00
>  LP2  LP2  LP3    1   111.000      460.00
> ;  CH2  CH1  CH2    1   109.500      460.00 ; no CH1 in DPC.
> ;  CH1   OS    C    1   120.000     418.00 ; no CH1 or C in DPC.
> ;  CH2   OS    C    1   120.000     418.00 ; no C in DPC.
> ;  CH3   OS    C    1   120.000      418.00 ; no C in DPC.
> ;   OS    C    O    1   124.000      502.00 ; no C in DPC.
> ;   OS    C  CH2    1   115.000      502.00 ; no C in DPC.
> ;    O    C  CH2    1   121.000      502.00 ; no C in DPC.
> ;    C  CH2  CH2    1   120.000      585.00 ; no C in DPC.
>
> [ moleculetype ]
> ; Name   nrexcl
> DPC      3
>
> [ atoms ]
> ;   nr    type   resnr  residu    atom    cgnr        charge          mass
>     1     LC3      1    DPC      C1       1         0.4        ;   
> qtot: 0.248, type formerly CH3
>     2     LC3      1    DPC      C2       1         0.4        ;   
> qtot: 0.496, type formerly CH3
>     3     LC3      1    DPC      C3       1         0.4        ;   
> qtot: 0.744, type formerly CH3
>     4     LNL      1    DPC      N4       1        -0.5        ;   
> qtot: 0.752, type formerly NL
>     5     LH2      1    DPC      C5       1         0.3        ;   
> qtot: 1, type formerly CH2
>     6     LC2      1    DPC      C6       1         0.4        ;   
> qtot: 1, type formerly CH2
>     7     LOS      1    DPC      O7       1        -0.8        ;   
> qtot: 0.64, type formerly OS
>     8     LP       1    DPC      P8       1         1.7        ;   
> qtot: 1.64, type formerly P
>     9     LOM      1    DPC      O9       1        -0.8        ;   
> qtot: 1, type formerly OM
>    10     LOM      1    DPC     O10       1        -0.8        ;   
> qtot: 0.36, type formerly OM
>    11     LOS      1    DPC     O11       1        -0.7        ;   
> qtot: 0, type formerly OS
>    12     LP2      1    DPC     C12       5             0      ;   
> qtot: 0, type formerly CH2
>    13     LP2      1    DPC     C13       6             0      ;   
> qtot: 0, type formerly CH2
>    14     LP2      1    DPC     C14       7             0      ;   
> qtot: 0, type formerly CH2
>    15     LP2      1    DPC     C15       8             0      ;   
> qtot: 0, type formerly CH2
>    16     LP2      1    DPC     C16       9             0      ;   
> qtot: 0, type formerly CH2
>    17     LP2      1    DPC     C17      10             0      ;   
> qtot: 0, type formerly CH2
>    18     LP2      1    DPC     C18      11             0      ;   
> qtot: 0, type formerly CH2
>    19     LP2      1    DPC     C19      12             0      ;   
> qtot: 0, type formerly CH2
>    20     LP2      1    DPC     C20      13             0      ;   
> qtot: 0, type formerly CH2
>    21     LP2      1    DPC     C21      14             0      ;   
> qtot: 0, type formerly CH2
>    22     LP2      1    DPC     C22      15             0      ;   
> qtot: 0, type formerly CH2
>    23     LP3      1    DPC     C23      16             0      ;   
> qtot: 0, type formerly CH3
>
> [ bonds ]
> ;  ai    aj funct
>    1     4     1
>    2     4     1
>    3     4     1
>    4     5     1
>    5     6     1
>    6     7     1
>    7     8     1
>    8     9     1
>    8    10     1
>    8    11     1
>   11    12     1
>   12    13     1
>   13    14     1
>   14    15     1
>   15    16     1
>   16    17     1
>   17    18     1
>   18    19     1
>   19    20     1
>   20    21     1
>   21    22     1
>   22    23     1
>
> [ pairs ]
> ;  ai    aj funct
> ; Here is the first copy.
>    1     6     1
>    2     6     1
>    3     6     1
>    4     7     1
>    5     8     1
>    6     9     1
>    6    10     1
>    6    11     1
>    7    12     1
>    8    13     1
>    9    12     1
>   10    12     1
>   11    14     1
> ;   12    15     1
> ;   13    16     1
> ;   14    17     1
> ;   15    18     1
> ;   16    19     1
> ;   17    20     1
> ;   18    21     1
> ;   19    22     1
> ;   20    23     1
> ; Here is the second copy.  Only use this with the halved LJ-14 epsilon
> values.
>    1     6     1
>    2     6     1
>    3     6     1
>    4     7     1
>    5     8     1
>    6     9     1
>    6    10     1
>    6    11     1
>    7    12     1
>    8    13     1
>    9    12     1
>   10    12     1
>   11    14     1
>
>
> [ angles ]
> ;  ai    aj    ak funct
>    1     4     2     1
>    1     4     3     1
>    1     4     5     1
>    2     4     3     1
>    2     4     5     1
>    3     4     5     1
>    4     5     6     1
>    5     6     7     1
>    6     7     8     1
>    7     8     9     1
>    7     8    10     1
>    7     8    11     1
>    9     8    10     1
>    9     8    11     1
>   10     8    11     1
>    8    11    12     1
>   11    12    13     1
>   12    13    14     1
>   13    14    15     1
>   14    15    16     1
>   15    16    17     1
>   16    17    18     1
>   17    18    19     1
>   18    19    20     1
>   19    20    21     1
>   20    21    22     1
>   21    22    23     1
>
> [ dihedrals ]
> ;  ai    aj    ak    al funct
>    1     4     5     6     1
>    4     5     6     7     1
>    5     6     7     8     1
>    6     7     8     9     1
>    7     8    11    12     1
>    8    11    12    13     1
>   11    12    13    14     1
>   12    13    14    15     3
>   13    14    15    16     3
>   14    15    16    17     3
>   15    16    17    18     3
>   16    17    18    19     3
>   17    18    19    20     3
>   18    19    20    21     3
>   19    20    21    22     3
>   20    21    22    23     3
>
>
> ffoplsaanb.itp:
>
> [ atomtypes ]
> ; full atom descriptions are available in ffoplsaa.atp
> ; name  bond_type    mass    charge   ptype          sigma      epsilon
> ; added by soo mei and devan on 4th June 2008
> LO LO 15.9994   0.000 A     0.296           0.878693
> LOM LOM 15.9994 0.000 A     0.296           0.878693
> LNL LNL 14.0067 0.000 A     0.324997        0.711373
> LC LC 12.0110 0.000 A      0.374986        0.439521
> LH1 LH1 13.0190 0.000 A     0.380015        0.334617
> LH2 LH2 14.0270 0.000 A     0.390504        0.493637
> LP LP 30.9738 0.000 A      0.374004        0.836713
> LOS LOS 15.9994 0.000 A     0.299989        0.879137
> LP2 LP2 14.0270 0.000 A     0.395994        0.38084
> LP3 LP3 15.0350 0.000 A     0.396006        0.568952
> LC3 LC3 15.0350 0.000 A     0.396012        0.606495
> LC2 LC2 14.0270 0.000 A     0.379979        0.493945
>
> ...
>
> [ pairtypes ]
> ; added by soo mei and devan on 4th June 2008
> ; i    j    func   sigma          halved_epsilon
> LO      LO      1       0.296038       0.0548205
> LO      LOM     1       0.296038       0.0548205
> LO      opls_116 1      0.306317       0.0473315
> LO      LNL     1       0.310178        0.0493787
> LO      LC      1       0.333186        0.0387945
> LO      LH1     1       0.335422       0.0338365
> LO      LH2     1       0.340009        0.0411152
> LO      LP      1       0.332746       0.0535345
> LO      LOS     1       0.297989       0.0548975
> LO      LP2     1       0.342367        0.0361464
> LO      LP3     1       0.342395        0.0441426
> LO      LC3     1       0.342385        0.0455935
> LO      LC2     1       0.335388        0.0411466
> LOM     LOM     1       0.296038       0.0548205
> LOM     opls_116 1      0.306317       0.0473315
> LOM     LNL     1       0.310178        0.0493787
> LOM     LC      1       0.333186        0.0387945
> LOM     LH1     1       0.335422       0.0338365
> LOM     LH2     1       0.340009        0.0411152
> LOM     LP      1       0.332746       0.0535345
> LOM     LOS     1       0.297989       0.0548975
> LOM     LP2     1       0.342367        0.0361464
> LOM     LP3     1       0.342395        0.0441426
> LOM     LC3     1       0.342385        0.0455935
> LOM     LC2     1       0.335388        0.0411466
> opls_116 LNL    1       0.320955        0.0426192
> opls_116 LC     1       0.344783        0.0334699
> opls_116 LH1    1       0.347079        0.0292101
> opls_116 LH2    1       0.351809        0.0355021
> opls_116 LP     1       0.344302       0.046215
> opls_116 LOS    1       0.308362        0.0473692
> opls_116 LP2    1       0.354267        0.0311978
> opls_116 LP3    1       0.354273        0.0381177
> opls_116 LC3    1       0.354276        0.0393613
> opls_116 LC2    1       0.347048        0.0355144
> LNL     LNL     1       0.325001       0.0444655
> LNL     LC      1       0.349124        0.0349218
> LNL     LH1     1       0.351449        0.0304739
> LNL     LH2     1       0.356251       0.0370305
> LNL     LP      1       0.348642        0.0482144
> LNL     LOS     1       0.312236        0.0494409
> LNL     LP2     1       0.358753        0.0325351
> LNL     LP3     1       0.358767        0.0397492
> LNL     LC3     1       0.358753        0.0410602
> LNL     LC2     1       0.35139       0.0370716
> LC      LC      1       0.375036        0.0274302
> LC      LH1     1       0.37751       0.0239465
> LC      LH2     1       0.382703        0.0290762
> LC      LP      1       0.374519        0.0378681
> LC      LOS     1       0.335418       0.038816
> LC      LP2     1       0.385354        0.0255642
> LC      LP3     1       0.385387       0.0312205
> LC      LC3     1       0.385369        0.032252
> LC      LC2     1       0.37751       0.0290941
> LH1     LH1     1       0.380041        0.0208895
> LH1     LH2     1       0.385255        0.0253742
> LH1     LP      1       0.377008        0.0330493
> LH1     LOS     1       0.337649        0.0338775
> LH1     LP2     1       0.387935       0.022305
> LH1     LP3     1       0.387966        0.0272392
> LH1     LC3     1       0.387944        0.0281396
> LH1     LC2     1       0.380031        0.0253867
> LH2     LH2     1       0.390543        0.0308181
> LH2     LP      1       0.382176        0.0401572
> LH2     LOS     1       0.342275        0.0411569
> LH2     LP2     1       0.393236        0.0270987
> LH2     LP3     1       0.393276       0.0330925
> LH2     LC3     1       0.393237        0.0342109
> LH2     LC2     1       0.385233        0.0308442
> LP      LP      1       0.373989       0.0523075
> LP      LOS     1       0.334959       0.053589
> LP      LP2     1       0.384828        0.0352884
> LP      LP3     1       0.384887        0.0430654
> LP      LC3     1       0.384866        0.0445026
> LP      LC2     1       0.376986        0.0401721
> LOS     LOS     1       0.299979       0.054944
> LOS     LP2     1       0.344642        0.0361853
> LOS     LP3     1       0.344676        0.0441854
> LOS     LC3     1       0.344657        0.0456467
> LOS     LC2     1       0.337624        0.0411842
> LP2     LP2     1       0.395957        0.0238272
> LP2     LP3     1       0.395999        0.0290924
> LP2     LC3     1       0.395992        0.0300486
> LP2     LC2     1       0.387888        0.0271217
> LP3     LP3     1       0.39601       0.0355533
> LP3     LC3     1       0.395997        0.0367273
> LP3     LC2     1       0.387918        0.0331251
> LC3     LC3     1       0.395987        0.0379324
> LC3     LC2     1       0.387907        0.0342131
> LC2     LC2     1       0.379982        0.0308754
>
>
> ffoplsaabon.itp:
>
> [ dihedraltypes ]
> ;  i    j    k    l   func     coefficients
> ; OPLS Fourier dihedraltypes translated to Gromacs Ryckaert-Bellemans form
> ; according to the formula in the Gromacs manual.
> ;added by soo mei and devan on 4th June 2008
>
> LP2 LP2  3   9.2789   12.156  -13.120 -3.0597 26.240 -31.495
> LH2 LH2  3   9.2789   12.156  -13.120 -3.0597 26.240 -31.495
>





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