[gmx-users] 1-4 Scaling Problem?

Xavier Periole X.Periole at rug.nl
Sat May 24 16:44:23 CEST 2008


> I am sufferering from a dmpc-bilayer-area shrinking problem. The fact is the 
>experimental area at 300K is 59.6A2, where I am always getting 54A2 or 55A2 
>area. The dmpc_npat.pdb along with lipi.itp and dmpc.itp was taken from the 
>Tieleman's site, and I tried to modify these with half-epsilon double 
>pairlist method to combine OPLS-BERGER. 
The OPLS-Berger Lipids use a cutoff for vdW a bit smaller than the 1.4 nm
you use! try 1.2 and 1.0 nm you have a larger are per lipid, which does
not really mean you have a better representation of the lipids but your
area will be more reasonable.
> 
> I am doing an initial minimisation (4500 steps with emtop 100) with flexible 
>SPC and then turing on SETTLE and LINCS i am doing a pr-dynamics for 250ps 
>and then MD run for 5ns. In the pr-dynamics, the area is around the original, 
>but after starting MD, the area starts shrinking in 50ps only, and become 
>continuous near 54A2. I know, 500ps is not sufficient for equilibration but 
>the area shows no sign to again increase.
> 
> The pr.mdp is:
> title               =  bilayer MD DMPC POSRES PURE
> cpp                 =  /usr/bin/cpp
> define              =  -DDMPCPOSRES
> constraints         =  all-bonds
> constraint_algorithm=  lincs  
> lincs_order         =  4 
> lincs_warnangle     =  30
> integrator          =  md
> dt                  =  0.002    ; ps !
> nsteps              =  125000    ; total 100 ps.
> nstcomm             =  1
> comm-mode           =  linear
> nstxout             =  5000
> nstvout             =  5000
> nstfout             =  5000
> nstenergy           =  1000
> nstlist             =  5
> ns_type             =  grid
> rlist               =  0.9
> rcoulomb            =  0.9
> vdwtype             =  Cut-off
> rvdw                =  1.4
> fourierspacing      =  0.12
> fourier_nx          =  0
> fourier_ny          =  0
> fourier_nz          =  0
> coulombtype         =  PME
> pme_order           =  4
> ewald_rtol          =  1e-5
> optimize_fft        =  yes
> ; Berendsen temperature coupling is on in two groups
> Tcoupl              =  berendsen
> tau_t               =  0.1           0.1
> tc-grps             =  DMPC          sol
> ref_t               =  300           300
> ; Pressure coupling is not on
> Pcoupl              =  berendsen
> tau_p               =  4.0           4.0
> compressibility     =  4.5e-5        4.5e-5
> ref_p               =  1.0           1.0
> pcoupltype          =  semiisotropic
> ; Generate velocites is on at 300 K.
> gen_vel             =  yes
> gen_temp            =  300.0
> gen_seed            =  173529
> 
> the md.mdp file is:
> 
> title               =  bilayer MD DMPC PURE
> cpp                 =  /usr/bin/cpp
> constraints         =  all-bonds
> constraint_algorithm=  lincs  
> lincs_order         =  4 
> lincs_warnangle     =  30
> integrator          =  md
> dt                  =  0.002    ; ps !
> nsteps              =  2500000     ; total 100 ps.
> nstcomm             =  1
> comm-mode           =  linear
> nstxout             =  5000
> nstvout             =  5000
> nstfout             =  5000
> nstenergy           =  1000
> nstlist             =  5
> ns_type             =  grid
> rlist               =  0.9
> rcoulomb            =  0.9
> vdwtype             =  Cut-off
> rvdw                =  1.4
> fourierspacing      =  0.12
> fourier_nx          =  0
> fourier_ny          =  0
> fourier_nz          =  0
> coulombtype         =  PME
> pme_order           =  4
> ewald_rtol          =  1e-5
> optimize_fft        =  yes
> ; Berendsen temperature coupling is on in two groups
> Tcoupl              =  berendsen
> tau_t               =  0.1           0.1
> tc-grps             =  DMPC          sol
> ref_t               =  300           300
> ; Pressure coupling is not on
> Pcoupl              =  berendsen
> tau_p               =  4.0           4.0
> compressibility     =  4.5e-5        4.5e-5
> ref_p               =  1.0           1.0
> pcoupltype          =  semiisotropic
> ; Generate velocites is on at 300 K.
> gen_vel             =  yes
> gen_temp            =  300.0
> gen_seed            =  173529
> 
> 
> the ffoplsaamd.itp is:
> #define _FF_OPLS
> #define _FF_OPLSAA
> 
> ; This force field uses a format that requires Gromacs 3.1.4 or later.
> ;
> ; References for the OPLS-AA force field: 
> ;
> ; W. L. Jorgensen, D. S. Maxwell, and J. Tirado-Rives,
> ; J. Am. Chem. Soc. 118, 11225-11236 (1996).
> ; W. L. Jorgensen and N. A. McDonald, Theochem 424, 145-155 (1998).
> ; W. L. Jorgensen and N. A. McDonald, J. Phys. Chem. B 102, 8049-8059 
>(1998).
> ; R. C. Rizzo and W. L. Jorgensen, J. Am. Chem. Soc. 121, 4827-4836 (1999).
> ; M. L. Price, D. Ostrovsky, and W. L. Jorgensen, J. Comp. Chem. (2001).
> ; E. K. Watkins and W. L. Jorgensen, J. Phys. Chem. A 105, 4118-4125 
>(2001)..
> ; G. A. Kaminski, R.A. Friesner, J.Tirado-Rives and W.L. Jorgensen, J. 
>Phys.. Chem. B 105, 6474 (2001).
> ;
> 
> [ defaults ]
> ; nbfunc	comb-rule	gen-pairs	fudgeLJ	fudgeQQ
> 1		3		yes		0.5	0.5
> 
> #include "ffoplsaanbmd.itp"
> #include "ffoplsaabon.itp"
> 
> the ffoplsaanbmd.itp is:
> 
> [ atomtypes ]
> ; full atom descriptions are available in ffoplsaa.atp
> ; name  bond_type    mass    charge   ptype          sigma      epsilon
>   LO   LO   1        15.9994      0.000       A    0.296      0.87721 
>  ;carbonyl O, OPLS
>  LOM   LOM  1        15.9994      0.000       A    0.296      0.87721 
>  ;carboxyl O, OPLS
>  LNL   LNL  1        14.0067      0.000       A    0.325      0.7107 
>  ;Nitrogen, OPLS
>   LC   LC   1        12.0110      0.000       A    0.37499    0.43967 
>  ;Carbonyl C, OPLS
>  LH1   LH1  1        13.0190      0.000       A    0.38002    0.33451 
>  ;CH1, OPLS
>  LH2   LH2  1        14.0270      0.000       A    0.3905     0.49353 
>   ;CH2, OPLS
>   LP   LP   1        30.9738      0.000       A    0.37400    0.83653 
>  ;phosphor, OPLS
>  LOS   LOS  1        15.9994      0.000       A    0.29285    1.0158 
>   ;ester oxygen, OPLS
>  LP2   LP2  1        14.0270      0.000       A    0.29999    0.87809   ;RB 
>CH2, Bergers LJ
>  LP3   LP3  1        15.0350      0.000       A    0.396      0.568937  ;RB 
>CH3, Bergers LJ
>  LC3   LC3  1        15.0350      0.000       A    0.39601    0.67601 
>  ;CH3, OPLS
>  LC2   LC2  1        14.0270      0.000       A    0.3798     0.49419 
>  ;CH2, OPLS
> .......................OPLS lines................................
> 
> [ pairtypes ]
>  ; i    j    func   sigma       epsilon
>   LO    LO     1 0.29604      0.05482
>   LO   LOM     1 0.29604      0.05482    
>   LO  opls_113 1 0.30632      0.04733    
>   LO   LNL     1 0.31018      0.04938    
>   LO    LC     1 0.33319      0.0388    
>   LO   LH1     1 0.33542      0.03383    
>   LO   LH2     1 0.34001      0.04111    
>   LO    LP     1 0.33275      0.05353    
>   LO   LOS     1 0.29799      0.0549     
>   LO   LP2     1 0.34237      0.03614    
>   LO   LP3     1 0.34239      0.04414    
>   LO   LC3     1 0.34238      0.04559    
>   LO   LC2     1 0.33539      0.04115    
>  LOM   LOM     1 0.29604      0.05482    
>  LOM  opls_113 1 0.30632      0.04733    
>  LOM   LNL     1 0.31018      0.04938    
>  LOM    LC     1 0.33319      0.0388    
>  LOM   LH1     1 0.33542      0.03383    
>  LOM   LH2     1 0.34001      0.04111    
>  LOM    LP     1 0.33275      0.05353    
>  LOM   LOS     1 0.29799      0.0549     
>  LOM   LP2     1 0.34237      0.03614    
>  LOM   LP3     1 0.34239      0.04414    
>  LOM   LC3     1 0.34238      0.04559    
>  LOM   LC2     1 0.33539      0.04115    
> opls_113 LNL     1 0.32095      0.04262    
> opls_113 LC     1 0.34478      0.03347    
> opls_113 LH1     1 0.34708      0.02921    
> opls_113 LH2     1 0.35181      0.0355     
> opls_113 LP     1 0.3443       0.04621    
> opls_113 LOS     1 0.30836      0.04737    
> opls_113 LP2     1 0.35427      0.0312     
> opls_113 LP3     1 0.35427      0.03812    
> opls_113 LC3     1 0.35428      0.03936    
> opls_113 LC2     1 0.34705      0.03551    
>  LNL    LNL     1 0.325        0.04446    
>  LNL    LC     1 0.34912      0.03492    
>  LNL   LH1     1 0.35145      0.03047    
>  LNL   LH2     1 0.35625      0.03703   
>  LNL    LP     1 0.34864      0.04822     
>  LNL   LOS     1 0.31224      0.04944    
>  LNL   LP2     1 0.35875      0.03254    
>  LNL   LP3     1 0.35877      0.03975    
>  LNL   LC3     1 0.35875      0.04106    
>  LNL   LC2     1 0.35139      0.03707    
>   LC    LC     1 0.37504      0.2743     
>   LC   LH1     1 0.37751      0.02395    
>   LC   LH2     1 0.3827       0.02908    
>   LC    LP     1 0.37452      0.03787    
>   LC   LOS     1 0.33542      0.03882    
>   LC   LP2     1 0.38535      0.25565    
>   LC   LP3     1 0.38539      0.03122    
>   LC   LC3     1 0.38537      0.03225    
>   LC   LC2     1 0.37751      0.02909    
>  LH1   LH1     1 0.38004      0.02089    
>  LH1   LH2     1 0.38525      0.02538     
>  LH1    LP     1 0.37701      0.03305    
>  LH1   LOS     1 0.33765      0.03388    
>  LH1   LP2     1 0.38793      0.0223    
>  LH1   LP3     1 0.38793      0.02724     
>  LH1   LC3     1 0.38794      0.02814    
>  LH1   LC2     1 0.38003      0.02539    
>  LH2   LH2     1 0.39054      0.03082    
>  LH2    LP     1 0.38218      0.04016    
>  LH2   LOS     1 0.34227      0.04115    
>  LH2   LP2     1 0.39324      0.0271     
>  LH2   LP3     1 0.39328      0.03309    
>  LH2   LC3     1 0.39324      0.03421    
>  LH2   LC2     1 0.38523      0.03085    
>   LP    LP     1 0.37399      0.0523    
>   LP   LOS     1 0.33496      0.05359     
>   LP   LP2     1 0.38483      0.03529    
>   LP   LP3     1 0.38489      0.04306    
>   LP   LC3     1 0.38487      0.04451    
>   LP   LC2     1 0.37699      0.04017    
>  LOS   LOS     1 0.29998      0.05495    
>  LOS   LP2     1 0.34464      0.36185    
>  LOS   LP3     1 0.34468      0.04419    
>  LOS   LC3     1 0.34466      0.04564    
>  LOS   LC2     1 0.33762      0.04118    
>  LP2   LP2     1 0.39596      0.02382    
>  LP2   LP3     1 0.396        0.02909    
>  LP2   LC3     1 0.39599      0.03005    
>  LP2   LC2     1 0.38789      0.02712    
>  LP3   LP3     1 0.39601      0.03556    
>  LP3   LC3     1 0.396        0.03673    
>  LP3   LC2     1 0.38792      0.03313    
>  LC3   LC3     1 0.39599      0.03793    
>  LC3   LC2     1 0.38791      0.03422    
>  LC2   LC2     1 0.37998      0.03088    
>                                                                               
> 
> [ dihedraltypes ]
> LP2 LP2  3   9.2789   12.156  -13.120 -3.0597 26.240 -31.495
> LH2 LH2  3   9.2789   12.156  -13.120 -3.0597 26.240 -31.495
> 
> the dmpcopls.itp is:
> 
> [ moleculetype ]
> 
> ;   name  nrexcl
>  DMPC       3
> 
> [ atoms ]
> ;     id   atype   resnr resname   aname    cgnr  charge    mass
>       1     LC3       1   DMPC      CN1       0  0.4000 15.0350
>       2     LC3       1   DMPC      CN2       0  0.4000 15.0350
>       3     LC3       1   DMPC      CN3       0  0.4000 15.0350
>       4     LNL       1   DMPC      NTM       0 -0.5000 14.0067
>       5     LH2       1   DMPC       CA       0  0.3000 14.0270
>       6     LC2       1   DMPC       CB       1  0.4000 14.0270
>       7     LOS       1   DMPC       OA       1 -0.8000 15.9994
>       8      LP       1   DMPC        P       1  1.7000 30.9738
>       9     LOM       1   DMPC       OB       1 -0.8000 15.9994
>      10     LOM       1   DMPC       OC       1 -0.8000 15.9994
>      11     LOS       1   DMPC       OD       1 -0.7000 15.9994
>      12     LC2       1   DMPC       CC       2  0.4000 14.0270
>      13     LH1       1   DMPC       CD       2  0.3000 13.0190
>      14     LOS       1   DMPC       OE       2 -0.7000 15.9994
>      15      LC       1   DMPC      C2A       2  0.7000 12.0110
>      16      LO       1   DMPC       OF       2 -0.7000 15.9994
>      17     LP2       1   DMPC      C2B       3  0.0000 14.0270
>      18     LP2       1   DMPC      C2C       4  0.0000 14.0270
>      19     LP2       1   DMPC      C2D       5  0.0000 14.0270
>      20     LP2       1   DMPC      C2E       6  0.0000 14.0270
>      21     LP2       1   DMPC      C2F       7  0.0000 14.0270
>      22     LP2       1   DMPC      C2G       8  0.0000 14.0270
>      23     LP2       1   DMPC      C2H       9  0.0000 14.0270
>      24     LP2       1   DMPC      C2I      10  0.0000 14.0270
>      25     LP2       1   DMPC      C2J      11  0.0000 14.0270
>      26     LP2       1   DMPC      C2K      12  0.0000 14.0270
>      27     LP2       1   DMPC      C2L      13  0.0000 14.0270
>      28     LP2       1   DMPC      C2M      14  0.0000 14.0270
>      29     LP3       1   DMPC      C2N      15  0.0000 15.0350
>      30     LC2       1   DMPC       CE      18  0.5000 14.0270
>      31     LOS       1   DMPC       OG      18 -0.7000 15.9994
>      32      LC       1   DMPC      C1A      18  0.8000 12.0110
>      33      LO       1   DMPC       OH      18 -0.6000 15.9994
>      34     LP2       1   DMPC      C1B      19  0.0000 14.0270
>      35     LP2       1   DMPC      C1C      20  0.0000 14.0270
>      36     LP2       1   DMPC      C1D      21  0.0000 14.0270
>      37     LP2       1   DMPC      C1E      22  0.0000 14.0270
>      38     LP2       1   DMPC      C1F      23  0.0000 14.0270
>      39     LP2       1   DMPC      C1G      24  0.0000 14.0270
>      40     LP2       1   DMPC      C1H      25  0.0000 14.0270
>      41     LP2       1   DMPC      C1I      26  0.0000 14.0270
>      42     LP2       1   DMPC      C1J      27  0.0000 14.0270
>      43     LP2       1   DMPC      C1K      28  0.0000 14.0270
>      44     LP2       1   DMPC      C1L      29  0.0000 14.0270
>      45     LP2       1   DMPC      C1M      30  0.0000 14.0270
>      46     LP3       1   DMPC      C1N      31  0.0000 15.0350
> 
> [ bonds ]
> ;     ai      aj    func          b0          cb
>       1       4       1 0.14700E+00 0.37660E+06
>       2       4       1 0.14700E+00 0.37660E+06
>       3       4       1 0.14700E+00 0.37660E+06
>       4       5       1 0.14700E+00 0.37660E+06
>       5       6       1 0.15300E+00 0.33470E+06
>       6       7       1 0.14300E+00 0.33470E+06
>       7       8       1 0.16100E+00 0.25100E+06
>       8       9       1 0.14800E+00 0.33470E+06
>       8      10       1 0.14800E+00 0.33470E+06
>       8      11       1 0.16100E+00 0.25100E+06
>      11      12       1 0.14300E+00 0.33470E+06
>      12      13       1 0.15300E+00 0.33470E+06
>      13      14       1 0.14300E+00 0.33470E+06
>      14      15       1 0.13600E+00 0.37660E+06
>      15      16       1 0.12300E+00 0.50210E+06
>      15      17       1 0.15300E+00 0.33470E+06
>      17      18       1 0.15300E+00 0.33470E+06
>      18      19       1 0.15300E+00 0.33470E+06
>      19      20       1 0.15300E+00 0.33470E+06
>      20      21       1 0.15300E+00 0.33470E+06
>      21      22       1 0.15300E+00 0.33470E+06
>      22      23       1 0.15300E+00 0.33470E+06
>      23      24       1 0.15300E+00 0.33470E+06
>      24      25       1 0.15300E+00 0.33470E+06
>      25      26       1 0.15300E+00 0.33470E+06
>      26      27       1 0.15300E+00 0.33470E+06
>      27      28       1 0.15300E+00 0.33470E+06
>      28      29       1 0.15300E+00 0.33470E+06
>      13      30       1 0.15300E+00 0.33470E+06
>      30      31       1 0.14300E+00 0.33470E+06
>      31      32       1 0.13600E+00 0.37660E+06
>      32      33       1 0.12300E+00 0.50210E+06
>      32      34       1 0.15300E+00 0.33470E+06
>      34      35       1 0.15300E+00 0.33470E+06
>      35      36       1 0.15300E+00 0.33470E+06
>      36      37       1 0.15300E+00 0.33470E+06
>      37      38       1 0.15300E+00 0.33470E+06
>      38      39       1 0.15300E+00 0.33470E+06
>      39      40       1 0.15300E+00 0.33470E+06
>      40      41       1 0.15300E+00 0.33470E+06
>      41      42       1 0.15300E+00 0.33470E+06
>      42      43       1 0.15300E+00 0.33470E+06
>      43      44       1 0.15300E+00 0.33470E+06
>      44      45       1 0.15300E+00 0.33470E+06
>      45      46       1 0.15300E+00 0.33470E+06
> 
> [ pairs ]
> ;Here is the first copy
>      ai      aj    func          c6         c12
>       1       6       1
>       2       6       1
>       3       6       1
>       4       7       1
>       5       8       1
>       6       9       1
>       6      10       1
>       6      11       1
>       7      12       1
>       8      13       1
>       9      12       1
>      10      12       1
>      11      14       1
>      11      30       1
>      12      15       1
>      12      31       1
>      13      16       1
>      13      17       1
>      13      32       1
>      14      18       1
>      14      31       1
>      15      19       1
>      15      30       1
>      16      18       1
>      30      33       1
>      30      34       1
>      31      35       1
>      32      36       1
>      33      35       1
> ; Here is the second copy. Only use this with the modified LJ-14 epsilon 
>values
>       1       6       1
>       2       6       1
>       3       6       1
>       4       7       1
>       5       8       1
>       6       9       1
>       6      10       1
>       6      11       1
>       7      12       1
>       8      13       1
>       9      12       1
>      10      12       1
>      11      14       1
>      11      30       1
>      12      15       1
>      12      31       1
>      13      16       1
>      13      17       1
>      13      32       1
>      14      18       1
>      14      31       1
>      15      19       1
>      15      30       1
>      16      18       1
>      30      33       1
>      30      34       1
>      31      35       1
>      32      36       1
>      33      35       1
> 
> [ angles ]
> ;     ai      aj      ak    func          t0          ct
>       1       4       2       1 0.10950E+03 0.46020E+03
>       1       4       3       1 0.10950E+03 0.46020E+03
>       1       4       5       1 0.10950E+03 0.46020E+03
>       2       4       3       1 0.10950E+03 0.46020E+03
>       2       4       5       1 0.10950E+03 0.46020E+03
>       3       4       5       1 0.10950E+03 0.46020E+03
>       4       5       6       1 0.10950E+03 0.46020E+03
>       5       6       7       1 0.10950E+03 0.46020E+03
>       6       7       8       1 0.12000E+03 0.39750E+03
>       7       8       9       1 0.10950E+03 0.39750E+03
>       7       8      10       1 0.10950E+03 0.39750E+03
>       7       8      11       1 0.10300E+03 0.39750E+03
>       9       8      10       1 0.12000E+03 0.58580E+03
>       9       8      11       1 0.10950E+03 0.39750E+03
>      10       8      11       1 0.10950E+03 0.39750E+03
>       8      11      12       1 0.12000E+03 0.39750E+03
>      11      12      13       1 0.11100E+03 0.46020E+03
>      12      13      14       1 0.10950E+03 0.46020E+03
>      12      13      30       1 0.10950E+03 0.46020E+03
>      14      13      30       1 0.10950E+03 0.46020E+03
>      13      14      15       1 0.12000E+03 0.41840E+03
>      14      15      16       1 0.12400E+03 0.50210E+03
>      14      15      17       1 0.11500E+03 0.50210E+03
>      16      15      17       1 0.12100E+03 0.50210E+03
>      15      17      18       1 0.11100E+03 0.46020E+03
>      17      18      19       1 0.11100E+03 0.46020E+03
>      18      19      20       1 0.11100E+03 0.46020E+03
>      19      20      21       1 0.11100E+03 0.46020E+03
>      20      21      22       1 0.11100E+03 0.46020E+03
>      21      22      23       1 0.11100E+03 0.46020E+03
>      22      23      24       1 0.11100E+03 0.46020E+03
>      23      24      25       1 0.11100E+03 0.46020E+03
>      24      25      26       1 0.11100E+03 0.46020E+03
>      25      26      27       1 0.11100E+03 0.46020E+03
>      26      27      28       1 0.11100E+03 0.46020E+03
>      27      28      29       1 0.11100E+03 0.46020E+03
>      13      30      31       1 0.11100E+03 0.46020E+03
>      30      31      32       1 0.12000E+03 0.41840E+03
>      31      32      33       1 0.12400E+03 0.50210E+03
>      31      32      34       1 0.11500E+03 0.50210E+03
>      33      32      34       1 0.12100E+03 0.50210E+03
>      32      34      35       1 0.11100E+03 0.46020E+03
>      34      35      36       1 0.11100E+03 0.46020E+03
>      35      36      37       1 0.11100E+03 0.46020E+03
>      36      37      38       1 0.11100E+03 0.46020E+03
>      37      38      39       1 0.11100E+03 0.46020E+03
>      38      39      40       1 0.11100E+03 0.46020E+03
>      39      40      41       1 0.11100E+03 0.46020E+03
>      40      41      42       1 0.11100E+03 0.46020E+03
>      41      42      43       1 0.11100E+03 0.46020E+03
>      42      43      44       1 0.11100E+03 0.46020E+03
>      43      44      45       1 0.11100E+03 0.46020E+03
>      44      45      46       1 0.11100E+03 0.46020E+03
> 
> [ dihedrals ]
> ; These are the improper dihedrals
> ;     ai      aj      ak      al    func          q0          cq
>      13      14      30      12       2 0.35264E+02 0.33470E+03
>      15      14      17      16       2 0.00000E+00 0.16740E+03
>      32      31      34      33       2 0.00000E+00 0.16740E+03
> 
> [ dihedrals ]
> ; These are the proper dihedrals
> ;     ai      aj      ak      al    func        phi0        cphi    mult
>       1       4       5       6       1 0.00000E+00 0.37656E+01       3
>       4       5       6       7       1 0.00000E+00 0.58576E+01       3
>       5       6       7       8       1 0.00000E+00 0.37656E+01       3
>       6       7       8      11       1 0.00000E+00 0.10460E+01       3
>       7       8      11      12       1 0.00000E+00 0.10460E+01       3
>       8      11      12      13       1 0.00000E+00 0.37656E+01       3
>      11      12      13      14       1 0.00000E+00 0.20920E+01       2
>      12      13      14      15       1 0.00000E+00 0.37656E+01       3
>      13      14      15      17       1 0.18000E+03 0.16736E+02       2
>      14      15      17      18       1 0.00000E+00 0.41840E+00       6
>      15      17      18      19       1 0.00000E+00 0.58576E+01       3
>      17      18      19      20       3
>      18      19      20      21       3
>      19      20      21      22       3
>      20      21      22      23       3
>      21      22      23      24       3
>      22      23      24      25       3
>      23      24      25      26       3
>      24      25      26      27       3
>      25      26      27      28       3
>      26      27      28      29       3
>      14      13      30      31       1 0.00000E+00 0.20920E+01       2
>      13      30      31      32       1 0.00000E+00 0.37656E+01       3
>      30      31      32      34       1 0.18000E+03 0.16736E+02       2
>      31      32      34      35       1 0.00000E+00 0.41840E+00       6
>      32      34      35      36       1 0.00000E+00 0.58576E+01       3
>      34      35      36      37       3
>      35      36      37      38       3
>      36      37      38      39       3
>      37      38      39      40       3
>      38      39      40      41       3
>      39      40      41      42       3
>      40      41      42      43       3
>      41      42      43      44       3
>      42      43      44      45       3
>      43      44      45      46       3
> 
> #ifdef DMPC_POSRES
> ; position restraints for DMPC
> [ position_restraints ]
> ; atom  type      fx      fy      fz
>     1     1   1000.0  1000.0  1000.0
>     2     1   1000.0  1000.0  1000.0
>     3     1   1000.0  1000.0  1000.0
>     4     1   1000.0  1000.0  1000.0
>     5     1   1000.0  1000.0  1000.0
>     6     1   1000.0  1000.0  1000.0
>     7     1   1000.0  1000.0  1000.0
>     8     1   1000.0  1000.0  1000.0
>     9     1   1000.0  1000.0  1000.0
>    10     1   1000.0  1000.0  1000.0
>    11     1   1000.0  1000.0  1000.0
>    12     1   1000.0  1000.0  1000.0
>    13     1   1000.0  1000.0  1000.0
>    14     1   1000.0  1000.0  1000.0
>    15     1   1000.0  1000.0  1000.0
>    16     1   1000.0  1000.0  1000.0
>    17     1   1000.0  1000.0  1000.0
>    18     1   1000.0  1000.0  1000.0
>    19     1   1000.0  1000.0  1000.0
>    20     1   1000.0  1000.0  1000.0
>    21     1   1000.0  1000.0  1000.0
>    22     1   1000.0  1000.0  1000.0
>    23     1   1000.0  1000.0  1000.0
>    24     1   1000.0  1000.0  1000.0
>    25     1   1000.0  1000.0  1000.0
>    26     1   1000.0  1000.0  1000.0
>    27     1   1000.0  1000.0  1000.0
>    28     1   1000.0  1000.0  1000.0
>    29     1   1000.0  1000.0  1000.0
>    30     1   1000.0  1000.0  1000.0
>    31     1   1000.0  1000.0  1000.0
>    32     1   1000.0  1000.0  1000.0
>    33     1   1000.0  1000.0  1000.0
>    34     1   1000.0  1000.0  1000.0
>    35     1   1000.0  1000.0  1000.0
>    36     1   1000.0  1000.0  1000.0
>    37     1   1000.0  1000.0  1000.0
>    38     1   1000.0  1000.0  1000.0
>    39     1   1000.0  1000.0  1000.0
>    40     1   1000.0  1000.0  1000.0
>    41     1   1000.0  1000.0  1000.0
>    42     1   1000.0  1000.0  1000.0
>    43     1   1000.0  1000.0  1000.0
>    44     1   1000.0  1000.0  1000.0
>    45     1   1000.0  1000.0  1000.0
>    46     1   1000.0  1000.0  1000.0
> #endif
> 
> Is my 1-4 scaling correct? I am confused about my topologies. Please help.
> 
> 
> Ms. Anindita Gayen
> 
> C/O Dr. Chaitali Mukhopadhyay
> 
> Senior Research Fellow
> 
> University of Calcutta
> 
> 
>      Best Jokes, Best Friends, Best Food and more. Go to 
>http://in.promos.yahoo.com/groups/bestofyahoo/
> 
> _______________________________________________
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-----------------------------------------------------
XAvier Periole - PhD

NMR & Molecular Dynamics Group
University of Groningen
The Netherlands
http://md.chem.rug.nl/~periole
-----------------------------------------------------



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