[gmx-users] Invalid atom number 6415 in indexfile

Liu Shiyong liushiyong at gmail.com
Wed Jan 21 20:36:02 CET 2009


Hi,

 I got an error when do grompp:   The input PDB file comes from the output
of GROMACS by trajconv command.

*Here is the log from grompp:*

Option     Filename  Type         Description
------------------------------------------------------------
  -f         em.mdp  Input, Opt!  grompp input file with MD parameters
 -po      mdout.mdp  Output       grompp input file with MD parameters
  -c r-l_103703_G53a6.minim_traj_withH_0.6.gro  Input        Generic
                                   structure: gro g96 pdb tpr tpb tpa xml
  -r       conf.gro  Input, Opt.  Generic structure: gro g96 pdb tpr tpb tpa
                                   xml
 -rb       conf.gro  Input, Opt.  Generic structure: gro g96 pdb tpr tpb tpa
                                   xml
  -n r-l_103703_G53a6.minim_traj_withH_0.6.ndx  Input, Opt!  Index file
-deshuf  deshuf.ndx  Output, Opt. Index file
  -p r-l_103703_G53a6.minim_traj_withH_0.6.top  Input        Topology file
 -pp  processed.top  Output, Opt. Topology file
  -o r-l_103703_G53a6.minim_traj_withH_0.6.input.tpr  Output       Generic
                                   run input: tpr tpb tpa xml
  -t       traj.trr  Input, Opt.  Full precision trajectory: trr trj
  -e       ener.edr  Input, Opt.  Generic energy: edr ene

Option       Type   Value   Description
------------------------------------------------------
-[no]h       bool   no      Print help info and quit
-[no]X       bool   no      Use dialog box GUI to edit command line options
-nice        int    0       Set the nicelevel
-[no]v       bool   yes     Be loud and noisy
-time        real   -1      Take frame at or first after this time.
-np          int    1       Generate statusfile for # nodes
-[no]shuffle bool   no      Shuffle molecules over nodes
-[no]sort    bool   no      Sort molecules according to X coordinate
-[no]rmvsbds bool   yes     Remove constant bonded interactions with virtual
                            sites
-load        string         Releative load capacity of each node on a
                            parallel machine. Be sure to use quotes around
                            the string, which should contain a number for
                            each node
-maxwarn     int    10      Number of warnings after which input processing
                            stops
-[no]check14 bool   no      Remove 1-4 interactions without Van der Waals
-[no]zero    bool   no      Set parameters for bonded interactions without
                            defaults to zero instead of generating an error
-[no]renum   bool   yes     Renumber atomtypes and minimize number of
                            atomtypes


Back Off! I just backed up mdout.mdp to ./#mdout.mdp.1#
checking input for internal consistency...
Generated 165 of the 1596 non-bonded parameter combinations
Excluding 3 bonded neighbours for Protein_A 1
Excluding 3 bonded neighbours for Protein_B 1
Excluding 3 bonded neighbours for Protein_D 1
Excluding 3 bonded neighbours for Protein_E 1
NOTE:
  System has non-zero total charge: -4.000003e+00

processing coordinates...
double-checking input for internal consistency...
WARNING 1 [file "r-l_103703_G53a6.minim_traj_withH_0.6.top", line 41]:
  With nstlist=0 atoms are only put into the box at step 0, therefore
  drifting atoms might cause the simulation to crash.
renumbering atomtypes...
converting bonded parameters...
initialising group options...
processing index file...
Making dummy/rest group for T-Coupling containing 6415 elements
Making dummy/rest group for Acceleration containing 6415 elements
Making dummy/rest group for Freeze containing 6415 elements

-------------------------------------------------------
Program grompp, VERSION 3.3.3
Source code file: ../../../../src/kernel/readir.c, line: 838

*Fatal error:
Invalid atom number 6415 in indexfile*
-------------------------------------------------------

"Bailed Out Of Edge Synchronization After 10,000 Iterations" (X/Motif)

                         :-)  G  R  O  M  A  C  S  (-:

                     Gnomes, ROck Monsters And Chili Sauce

                            :-)  VERSION 3.3.3  (-:


      Written by David van der Spoel, Erik Lindahl, Berk Hess, and others.
       Copyright (c) 1991-2000, University of Groningen, The Netherlands.
             Copyright (c) 2001-2008, The GROMACS development team,
            check out http://www.gromacs.org for more information.

         This program is free software; you can redistribute it and/or
          modify it under the terms of the GNU General Public License
         as published by the Free Software Foundation; either version 2
             of the License, or (at your option) any later version.

                                :-)  grompp  (-:

creating statusfile for 1 node...
calling /usr/bin/cpp...
processing topology...
#   G96BONDS:   6557
#  G96ANGLES:   9621
#      PDIHS:   3328
#      IDIHS:   3469
#       LJ14:   10148

*Here is the log from make_ndx:*


                         :-)  G  R  O  M  A  C  S  (-:

                     Gnomes, ROck Monsters And Chili Sauce

                            :-)  VERSION 3.3.3  (-:


      Written by David van der Spoel, Erik Lindahl, Berk Hess, and others.
       Copyright (c) 1991-2000, University of Groningen, The Netherlands.
             Copyright (c) 2001-2008, The GROMACS development team,
            check out http://www.gromacs.org for more information.

         This program is free software; you can redistribute it and/or
          modify it under the terms of the GNU General Public License
         as published by the Free Software Foundation; either version 2
             of the License, or (at your option) any later version.

                               :-)  make_ndx  (-:

Option     Filename  Type         Description
------------------------------------------------------------
  -f r-l_103703_G53a6.minim_traj_withH.pdb  Input, Opt!  Generic structure:
                                   gro g96 pdb tpr tpb tpa xml
  -n      index.ndx  Input, Opt., Mult. Index file
  -o r-l_103703_G53a6.minim_traj_withH_0.6.ndx  Output       Index file

Option       Type   Value   Description
------------------------------------------------------
-[no]h       bool   no      Print help info and quit
-[no]X       bool   no      Use dialog box GUI to edit command line options
-nice        int    0       Set the nicelevel
-natoms      int    0       set number of atoms (default: read from
                            coordinate or index file)


Reading structure file
Opening library file /usr/share/gromacs/top/aminoacids.dat

gcq#137: "Shit Happens" (Pulp Fiction)

Going to read 0 old index file(s)
Analysing residue names:
There are:     0      OTHER residues
There are:   603    PROTEIN residues
There are:     0        DNA residues
Analysing Protein...

  0 System              :  6416 atoms
  1 Protein             :  6416 atoms
  2 Protein-H           :  4898 atoms
  3 C-alpha             :   603 atoms
  4 Backbone            :  1809 atoms
  5 MainChain           :  2416 atoms
  6 MainChain+Cb        :  2982 atoms
  7 MainChain+H         :  2997 atoms
  8 SideChain           :  3419 atoms
  9 SideChain-H         :  2482 atoms

 nr : group       !   'name' nr name   'splitch' nr    Enter: list groups
 'a': atom        &   'del' nr         'splitres' nr   'l': list residues
 't': atom type   |   'keep' nr        'splitat' nr    'h': help
 'r': residue         'res' nr         'chain' char
 "name": group        'case': case sensitive           'q': save and quit

>
Removed group 1 'Protein'
Removed group 2 'Protein-H'
Removed group 3 'C-alpha'
Removed group 4 'Backbone'
Removed group 5 'MainChain'
Removed group 6 'MainChain+Cb'
Removed group 7 'MainChain+H'
Removed group 8 'SideChain'
Removed group 9 'SideChain-H'

>
Found 2378 atoms with chain identifiers D AND E

  1 chDANDE             :  2378 atoms

>
Found 4038 atoms with chain identifiers A AND B

  2 chAANDB             :  4038 atoms

>


-- 
Shiyong Liu
Postdoc
center for bioinformatics in the university of kansas
Lab: (785)864-1962
Email: syliu at ku.edu (shiyongliu at ku.edu or liushiyong at ku.edu)
Homepage: http://www.people.ku.edu/~syliu
Lab:    http://vakser.bioinformatics.ku.edu/people
Phone:      (785) 864-1962
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