[gmx-users] molecule index

Justin A. Lemkul jalemkul at vt.edu
Mon Sep 21 02:32:58 CEST 2009



Jamie Seyed wrote:
> Yes Thanks Justin, should I just use the linear part of msd for fit even 
> if it is short (like 14ps from 200ps)? Thanks/Jamie
> 

Yes, the linear portion of the graph should be used for MSD analysis.  200 ps is 
somewhat short, but might be sufficient for water.

-Justin

> On Sun, Sep 20, 2009 at 8:02 PM, Justin A. Lemkul <jalemkul at vt.edu 
> <mailto:jalemkul at vt.edu>> wrote:
> 
> 
> 
>     Jamie Seyed wrote:
> 
>         Thanks Mark,
>         here are the links.
>         http://www.psc.edu/general/software/packages/gromacs/online/water.html
>          http://www.mail-archive.com/gmx-users@gromacs.org/msg07653.html
>          I tried your advice with 1 water molecule and since it worked I
>         tried it with SOL.ndx, but I got
>         D[SOL] -0.0333(+/- 0.0370) 1e-5 cm^2/s
>         It seems not reasonable to me (negative and its error is bigger
>         than itself). Any idea?
> 
> 
>     Probably inadequate sampling, either from the number of frames
>     saved, or from the length of the simulation itself.
> 
>     -Justin
> 
>         Thanks in Advance/Jamie
> 
> 
>         On Sun, Sep 20, 2009 at 7:02 PM, Mark Abraham
>         <Mark.Abraham at anu.edu.au <mailto:Mark.Abraham at anu.edu.au>
>         <mailto:Mark.Abraham at anu.edu.au
>         <mailto:Mark.Abraham at anu.edu.au>>> wrote:
> 
>            Jamie Seyed wrote:
> 
>                Hi Mark,
>                Thanks for the puzzling answer. I tried the index file
>         counting
>                the number of water molecule but I got an error.
>                "Fatal error:
>                The index group does not consist of whole molecules"
> 
> 
>            OK so apparently it wants atom indices for whole molecules. That
>            seems much more plausible to me than David's advice you quoted
>            (please give URLs to such quotes in future, perhaps you took
>         it out
>            of context...)
> 
>            So try giving it atom indices for a whole water molecule as an
>            experiment. Seems like an obvious try to me :-) Then, scale
>         it up if
>            it seems to work.
> 
> 
>                I also did not understand what you pointed out about "One of
>                these sets of numbers is larger than the other" ...
> 
> 
>            One of the set of atom indices in a protein and the set of
>         molecule
>            indices in a solvated protein will have more members than the
>         other.
>            Instead of asking which is right, you might try out a case
>         that will
>            fail under one assumption and work under the other :-) Then
>         perhaps
>            ask a question with some evidence behind it.
> 
> 
>                questions:
>                1) for msd calculation for water molecules, do I need really
>                make index file for all waters,
>                2) What I made (count the water molecules in a [mymolecules]
>                index file), did not work. How I suppose to do make that?
>                3) In getting started-Water page that I found in google
>         it only
>                say "g_msd -n index" and did not explain which index... how
>                about if I use oxygen.ndx file??
> 
> 
>            Provide links, please. We're not going to duplicate your
>         googling.
> 
>            Mark
> 
>                I really appreciate your help. Many Thanks in Advance/Jamie
> 
> 
> 
>                 On Sun, Sep 20, 2009 at 3:33 PM, Mark Abraham
>                <Mark.Abraham at anu.edu.au <mailto:Mark.Abraham at anu.edu.au>
>         <mailto:Mark.Abraham at anu.edu.au <mailto:Mark.Abraham at anu.edu.au>>
>                <mailto:Mark.Abraham at anu.edu.au
>         <mailto:Mark.Abraham at anu.edu.au>
>                <mailto:Mark.Abraham at anu.edu.au
>         <mailto:Mark.Abraham at anu.edu.au>>>> wrote:
> 
>                   Jamie Seyed wrote:
> 
>                       Dear all,
>                       I want to calculate the msd using g_msd. I have a
>                macromolecule
>                       and some (let say 100) water molecules and I want
>         to use
>                msd for
>                       water only. from the manual and list I think I need to
>                make an
>                       index for molecules (water) and use -mol option.
>                        From David's answer on the list I found
>                       -----------------------------------------------------
>                       "If you know which molecules they are then you
>         just write
>                in a
>                       text editor:
>                       [ mymolecules ]
>                       3 5 19 23 45 109"
>                       ----------------------------------------------------
>                       My question is: let say the macromolecule is
>         coming first
>                in the
>                       pdb file and then there are water molecules. If I
>         want to
>                make
>                       an index for only waters, according to this
>         advice, should I
>                       write 2 3 4... 100  (just remove 1 that goes for
>                macromolecule)
>                       I am wondering if there is a confliction between these
>                       numbers(refer to molecules) and atom numbers of
>                       macromolecule.... Would you please clarify this
>         for me??
> 
> 
>                   Try it and see. One of these sets of numbers is larger
>         than the
>                   other, and if you use a number in that interval you'll
>         either
>                get an
>                   error or success, either of which will answer your
>         question.
> 
>                   Mark
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> 
>     -- 
>     ========================================
> 
>     Justin A. Lemkul
>     Ph.D. Candidate
>     ICTAS Doctoral Scholar
>     Department of Biochemistry
>     Virginia Tech
>     Blacksburg, VA
>     jalemkul[at]vt.edu <http://vt.edu/> | (540) 231-9080
>     http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

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