[gmx-users] pdb2gmx stop at "AtomType 1"(Justin)

Justin A. Lemkul jalemkul at vt.edu
Sun Oct 17 01:28:05 CEST 2010



英雄不再寂寞 wrote:
> Date: Sat, 16 Oct 2010 08:36:00 -0400
> From: "Justin A. Lemkul" <jalemkul at vt.edu>
> Subject: Re: [gmx-users] pdb2gmx stop at "AtomType 1"
> To: Discussion list for GROMACS users <gmx-users at gromacs.org>
> Message-ID: <4CB99C30.50105 at vt.edu>
> Content-Type: text/plain; charset=x-gbk; format=flowed
> 英雄不再寂寞 wrote:
>  > Dear gmx-users,
>  >   I have added the required residue groups in the rtp file. Then I run
>  > the pdb2gmx using the following line for the top file, only to stop at "
>  > AtomType 1". No any message is given. I can not think out what is
>  > happening at all. Please help me with this problem, thanks a lot. Note
>  > that I am using gmx-4.5.
> 
> Q: Did you also add an entry in residuetypes.dat and aminoacids.hdb (if 
> necessary)?
> 
> -Justin
>  
> A: Yes, I added the entry in residuetypes.dat like:
> DET Organic
> 

To what does "Organic" refer?  Is that even a recognized keyword?  What is the 
nature of the residue you've added?  Does adding "-debug 1" to your pdb2gmx 
command line provide any useful information in the resulting .log file?

-Justin

>  >   Chaofu Wu, Dr.
>  > 
>  > xiaowu759 at linux-s38y:~/workshop
>  > <mailto:xiaowu759 at linux-s38y:~/workshop>> pdb2gmx -f deta.pdb -o
>  > deta.gro -p deta.top -i deta.itp -ter
>  >                          :-)  G  R  O  M  A  C  S  (-:
>  >                Gromacs Runs One Microsecond At Cannonball Speeds
>  >                             :-)  VERSION 4.5.1  (-:
>  >         Written by Emile Apol, Rossen Apostolov, Herman J.C. Berendsen,
>  >       Aldert van Buuren, P盲r Bjelkmar, Rudi van Drunen, Anton Feenstra,
>  >         Gerrit Groenhof, Peter Kasson, Per Larsson, Peiter Meulenhoff,
>  >           Teemu Murtola, Szilard Pall, Sander Pronk, Roland Schultz,
>  >                 Michael Shirts, Alfons Sijbers, Peter Tieleman,
>  >                Berk Hess, David van der Spoel, and Erik Lindahl.
>  >        Copyright (c) 1991-2000, University of Groningen, The Netherlands.
>  >             Copyright (c) 2001-2010, The GROMACS development team at
>  >         Uppsala University & The Royal Institute of Technology, Sweden.
>  >             check out http://www.gromacs.org for more information.
>  >          This program is free software; you can redistribute it and/or
>  >           modify it under the terms of the GNU General Public License
>  >          as published by the Free Software Foundation; either version 2
>  >              of the License, or (at your option) any later version.
>  >                                :-)  pdb2gmx  (-:
>  > Option     Filename  Type         Description
>  > ------------------------------------------------------------
>  >   -f       deta.pdb  Input        Structure file: gro g96 pdb tpr etc.
>  >   -o       deta.gro  Output       Structure file: gro g96 pdb etc.
>  >   -p       deta.top  Output       Topology file
>  >   -i       deta.itp  Output       Include file for topology
>  >   -n      clean.ndx  Output, Opt. Index file
>  >   -q      clean.pdb  Output, Opt. Structure file: gro g96 pdb etc.
>  > Option       Type   Value   Description
>  > ------------------------------------------------------
>  > -[no]h       bool   no      Print help info and quit
>  > -[no]version bool   no      Print version info and quit
>  > -nice        int    0       Set the nicelevel
>  > -chainsep    enum   id_or_ter  Condition in PDB files when a new 
> chain and
>  >                             molecule_type should be started: id_or_ter,
>  >                             id_and_ter, ter, id or interactive
>  > -ff          string select  Force field, interactive by default. Use 
> -h for
>  >                             information.
>  > -water       enum   select  Water model to use: select, none, spc, spce,
>  >                             tip3p, tip4p or tip5p
>  > -[no]inter   bool   no      Set the next 8 options to interactive
>  > -[no]ss      bool   no      Interactive SS bridge selection
>  > -[no]ter     bool   yes     Interactive termini selection, iso charged
>  > -[no]lys     bool   no      Interactive Lysine selection, iso charged
>  > -[no]arg     bool   no      Interactive Arganine selection, iso charged
>  > -[no]asp     bool   no      Interactive Aspartic Acid selection, iso 
> charged
>  > -[no]glu     bool   no      Interactive Glutamic Acid selection, iso 
> charged
>  > -[no]gln     bool   no      Interactive Glutamine selection, iso neutral
>  > -[no]his     bool   no      Interactive Histidine selection, iso checking
>  >                             H-bonds
>  > -angle       real   135     Minimum hydrogen-donor-acceptor angle for a
>  >                             H-bond (degrees)
>  > -dist        real   0.3     Maximum donor-acceptor distance for a H-bond
>  > (nm)
>  > -[no]una     bool   no      Select aromatic rings with united CH atoms on
>  >                             Phenylalanine, Tryptophane and Tyrosine
>  > -[no]ignh    bool   no      Ignore hydrogen atoms that are in the pdb 
> file
>  > -[no]missing bool   no      Continue when atoms are missing, dangerous
>  > -[no]v       bool   no      Be slightly more verbose in messages
>  > -posrefc     real   1000    Force constant for position restraints
>  > -vsite       enum   none    Convert atoms to virtual sites: none, 
> hydrogens
>  >                             or aromatics
>  > -[no]heavyh  bool   no      Make hydrogen atoms heavy
>  > -[no]deuterate bool no      Change the mass of hydrogens to 2 amu
>  > -[no]chargegrp bool yes     Use charge groups in the rtp file
>  > -[no]cmap    bool   yes     Use cmap torsions (if enabled in the rtp 
> file)
>  > -[no]renum   bool   no      Renumber the residues consecutively in the
>  > output
>  > -[no]rtpres  bool   no      Use rtp entry names as residue names
>  >
>  > Select the Force Field:
>  >  From current directory:
>  >  1: CNT_OPLS-AA/L all-atom force field (2001 aminoacid dihedrals)
>  >  2: OPLS-AA/L all-atom force field (2001 aminoacid dihedrals)
>  >  From '/usr/local/gromacs/share/gromacs/top':
>  >  3: AMBER03 force field (Duan et al., J. Comp. Chem. 24, 1999-2012, 2003)
>  >  4: AMBER94 force field (Cornell et al., JACS 117, 5179-5197, 1995)
>  >  5: AMBER96 force field (Kollman et al., Acc. Chem. Res. 29, 461-469, 
> 1996)
>  >  6: AMBER99 force field (Wang et al., J. Comp. Chem. 21, 1049-1074, 2000)
>  >  7: AMBER99SB force field (Hornak et al., Proteins 65, 712-725, 2006)
>  >  8: AMBER99SB-ILDN force field (Lindorff-Larsen et al., Proteins 78,
>  > 1950-58, 2010)
>  >  9: AMBERGS force field (Garcia & Sanbonmatsu, PNAS 99, 2782-2787, 2002)
>  > 10: CHARMM27 all-atom force field (with CMAP) - version 2.0beta
>  > 11: GROMOS96 43a1 force field
>  > 12: GROMOS96 43a2 force field (improved alkane dihedrals)
>  > 13: GROMOS96 45a3 force field (Schuler JCC 2001 22 1205)
>  > 14: GROMOS96 53a5 force field (JCC 2004 vol 25 pag 1656)
>  > 15: GROMOS96 53a6 force field (JCC 2004 vol 25 pag 1656)
>  > 16: OPLS-AA/L all-atom force field (2001 aminoacid dihedrals)
>  > 17: [DEPRECATED] Encad all-atom force field, using full solvent charges
>  > 18: [DEPRECATED] Encad all-atom force field, using scaled-down vacuum
>  > charges
>  > 19: [DEPRECATED] Gromacs force field (see manual)
>  > 20: [DEPRECATED] Gromacs force field with hydrogens for NMR
>  > 16
>  > Using the Oplsaa force field in directory oplsaa.ff
>  > Opening force field file oplsaa.ff/watermodels.dat
>  > Select the Water Model:
>  >  1: TIP4P  TIP 4-point, recommended
>  >  2: TIP3P  TIP 3-point
>  >  3: TIP5P  TIP 5-point
>  >  4: SPC    simple point charge
>  >  5: SPC/E  extended simple point charge
>  >  6: None
>  > 6
>  > Opening force field file oplsaa.ff/aminoacids.r2b
>  > Reading deta.pdb...
>  > Read 20 atoms
>  > Analyzing pdb file
>  > Splitting PDB chains based on TER records or changing chain id.
>  > There are 1 chains and 0 blocks of water and 1 residues with 20 atoms
>  >   chain  #res #atoms
>  >   1 ' '     1     20
>  > All occupancies are one
>  > Opening force field file oplsaa.ff/atomtypes.atp
>  > Atomtype 1
>  > 
>  >
> 
> -- 
> ========================================
> 
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> MILES-IGERT Trainee
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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