[gmx-users] Reg; MD of DCE part II

Justin A. Lemkul jalemkul at vt.edu
Tue Oct 26 13:30:32 CEST 2010



vinothkumar mohanakrishnan wrote:
> Hi Justin
> 
> Thank you very much for your comments. I actually found that after 
> posting the question. sorry I have a new question i did NPT 
> equilibration of DCE molecules and the average pressure turns out to be 
> 3.4726 (bar) where as i want the pressure it to be 1 bar. what further i 
> need to do?.below is what i got from the g_energy command.
> 

Do more equilibration.  Clearly your system is not equilibrated if it has not 
reached the desired equilibrium conditions.

-Justin

> Energy                      Average       RMSD     Fluct.      Drift  
> Tot-Drift
> -------------------------------------------------------------------------------
> Pressure (bar)              3.47263    767.297    765.878     0.8078    
> 161.561
> 
>  below is my mdp file any help is highly appreciated
> 
> title        = DCE NVT equilibration
> cpp        = usr/bin/cpp
> integrator    = md       
> nsteps        = 200000 
> dt        = 0.001      
> nstxout        = 1000     
> nstvout        = 1000       
> nstenergy    = 1000       
> nstlog        = 1000       
> constraint_algorithm     = shake  
> constraints        = none   
> unconstrained_start    = yes  
> shake_tol        = 0.0001
> morse            = no
> ns_type        = grid 
> nstlist        = 5      
> rlist        = 1.0       
> coulombtype    = PME    
> rcoulomb    = 1.0      
> vdwtype        = Cut-off    
> rvdw        = 1.0    
> pme_order    = 4      
> fourierspacing    = 0.16     
> pbc        = xyz      
> tcoupl        = V-rescale    
> tc-grps        = system 
> tau_t        = 0.1     
> ref_t        = 300    
> pcoupl        = Parrinello-Rahman 
> pcoupltype    = semiisotropic           
> tau_p        = 2.0 2.0      
> ref_p        = 1.0 1.0      
> compressibility = 0.0 4.5e-5 
> DispCorr    = Enerpres 
> gen_vel        = yes     
> gen_temp    = 300    
> gen_seed    = 173529
> 
> Regards
> Vinoth
> 
> 
> 
> On Tue, Oct 26, 2010 at 4:34 PM, Justin A. Lemkul <jalemkul at vt.edu 
> <mailto:jalemkul at vt.edu>> wrote:
> 
> 
> 
>     vinothkumar mohanakrishnan wrote:
> 
>         Hi Dallas / all
> 
>         I looked up at the pressure coupling section and i found how to
>         keep the box size same for two axis and change only one axis (i
>         used semiisotropic pressure coupling type) but when i did that
>         the average pressure i get after the run is negative where as i
>         want it be close to 1 bar?. i dont know what might have gone wrong.
> 
>         Energy                      Average       RMSD     Fluct.    
>          Drift  Tot-Drift
>         -------------------------------------------------------------------------------
>         Pressure (bar)             -661.673    675.593    674.292
>          -0.725677   -145.136
> 
>         similary when i give g_energy -f npt.edr -o density.xvg the
>         density option is not there to select it interactively. hence i
>         am not able to plot the density graph.why?
> 
> 
>     All of the above suggests you've gotten your files confused and
>     you're analyzing an .edr file from an NVT run, not NPT.  If indeed
>     you did NPT, the density term would appear below, along with
>     "Volume."  The only time when these terms are not written is if the
>     volume of the unit cell is static (i.e., NVT).
> 
> 
> 
>         1  Bond             2  Angle            3  Ryckaert-Bell.   4
>          LJ-14        5  Coulomb-14       6  LJ-(SR)          7
>          Disper.-corr.    8  Coulomb-(SR) 9  Coul.-recip.    10
>          Potential       11  Kinetic-En.     12  Total-Energy 13
>          Conserved-En.   14  Temperature     15  Pressure-(bar)  16
>          Vir-XX       17  Vir-XY          18  Vir-XZ          19  Vir-YX
>                  20  Vir-YY       21  Vir-YZ          22  Vir-ZX        
>          23  Vir-ZY          24  Vir-ZZ       25  Pres-XX-(bar)   26
>          Pres-XY-(bar)   27  Pres-XZ-(bar)   28  Pres-YX-(bar)
>         29  Pres-YY-(bar)   30  Pres-YZ-(bar)   31  Pres-ZX-(bar)   32
>          Pres-ZY-(bar)
>         33  Pres-ZZ-(bar)   34  #Surf*SurfTen   35  Mu-X            36
>          Mu-Y         37  Mu-Z            38  T-System        39
>          Lamb-System
> 
>          and i have given my mdp file below. any help is highly appreciated.
> 
>         title        = DCE NPT equilibration
>         integrator    = md       nsteps        = 200000
>         dt        = 0.001      nstxout        = 1000      nstvout      
>          = 1000       nstenergy    = 1000       nstlog        = 1000    
>           constraint_algorithm = lincs  constraints    = all-bonds  
>         lincs_iter    = 1      lincs_order    = 4    ns_type        =
>         grid   nstlist        = 5  rlist        = 1.0      rcoulomb    =
>         1.0  rvdw        = 1.0   coulombtype    = PME  pme_order    = 4
>              fourierspacing    = 0.16   tcoupl        = V-rescale
>          tc-grps        = system  tau_t        = 0.1    ref_t        =
>         300        pcoupl        = Parrinello-Rahman pcoupltype    =
>         semiisotropic  tau_p        = 2.0 2.0    ref_p        = 1.0 1.0
>               compressibility = 0.0 4.5e-5
> 
> 
>     For what its worth, if this is your initial equilibration, using
>     Parrinello-Rahman coupling may not be your best choice.  If your
>     system is far from equilibrated, the P-R method (in my experience)
>     can allow for wide oscillations, and ultimately system instability.
>      Nothing wrong with Berendsen for your initial equilibration.
> 
>     But again, if pressure coupling was actually used, you'd have Volume
>     and Density terms in the .edr file, along with a more sensible value
>     of pressure.
> 
>     -Justin
> 
>         pbc        = xyz  DispCorr    = EnerPres   gen_vel        = no    
>         Regards
>         Vinoth
> 
> 
>         On Tue, Oct 26, 2010 at 5:33 AM, Dallas Warren
>         <Dallas.Warren at monash.edu <mailto:Dallas.Warren at monash.edu>
>         <mailto:Dallas.Warren at monash.edu
>         <mailto:Dallas.Warren at monash.edu>>> wrote:
> 
>             >I performed md simulation of 108 dichloroethane molecules in
>            isothermal-isobaric ensemble with the box size
> 
>             >3.002*2.17*2.17. after the simulation my box size has changed
>            considerably from the initial size to 2.87882 2.08095
> 
>             >2.08095.why this happens?
> 
>            
>            Density was obviously too lower under the conditions of the
>            simulation.  Look at the change of pressure, volume and
>         density for
>            the box during that simulation.
> 
>            
>             >my second question is can i have a control over the box
>         dimensions
>            changes during md (i.e when the box dimensions
> 
>             >changes during md i want to change it only on one axis(say x
>            axis) and want to keep the length of the other two axis (say
> 
>             >y and z) same as that of my initial box size? any help is
>         highly
>            apprecited.
> 
>            
>            Look at the pressure coupling settings, look at anisotropic
>         pressure
>            coupling.
> 
>            
>            Catch ya,
> 
>            Dr. Dallas Warren
> 
>            Medicinal Chemistry and Drug Action
> 
>            Monash Institute of Pharmaceutical Sciences, Monash University
>            381 Royal Parade, Parkville VIC 3010
>            dallas.warren at monash.edu <mailto:dallas.warren at monash.edu>
>         <mailto:dallas.warren at monash.edu <mailto:dallas.warren at monash.edu>>
> 
> 
>            +61 3 9903 9304
>            ---------------------------------
>            When the only tool you own is a hammer, every problem begins to
>            resemble a nail.
> 
>            
>            
> 
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> 
>     -- 
>     ========================================
> 
>     Justin A. Lemkul
>     Ph.D. Candidate
>     ICTAS Doctoral Scholar
>     MILES-IGERT Trainee
>     Department of Biochemistry
>     Virginia Tech
>     Blacksburg, VA
>     jalemkul[at]vt.edu <http://vt.edu> | (540) 231-9080
>     http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

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