[gmx-users] Grompp Error : Segmentation fault

Justin A. Lemkul jalemkul at vt.edu
Tue Sep 28 18:23:07 CEST 2010



Roland Schulz wrote:
> Please make sure that the error exists in the latest version. Either 
> 4.5.1 or if you know how please check the latest GIT version 
> from release-4-5-patches. If you still get the error with the latest 
> version, please file a bugzilla report and attach all required files.
> 

My first guess would be that these lines are problematic:

; Include TFO topology
#include TFO_amber99.itp

The .itp filename should be enclosed in quotes.

-Justin

> On Tue, Sep 28, 2010 at 9:01 AM, Alep Latep <m.alif.m.latif at gmail.com 
> <mailto:m.alif.m.latif at gmail.com>> wrote:
> 
>     Dear Gromacs Users and Developers,
> 
>     I'm currently using Gromacs 4.5 on a Fedora 12 i686 to performed my
>     ionic liquid MD simulation using amber 99 force field. I got my
>     topology from topolbuild v 1.3. I successfully minimized both cation
>     and anion separately using cg and steep. Then I include the anion in
>     the cation system using genbox. I got error from grompp like this: 
>     ------------------------------------------------------------------------------
>     Back Off! I just backed up mdout.mdp to ./#mdout.mdp.3#
>     checking input for internal consistency...
>     processing topology...
>     Generated 2211 of the 2211 non-bonded parameter combinations
>     Generating 1-4 interactions: fudge = 0.5
>     Generated 2211 of the 2211 1-4 parameter combinations
>     Segmentation fault (core dumped)
>     [alif at NeoX MD1]$ 
>     ---------------------------------------------------------------------------------
>     Still cant find my answer in the mailing list. Sorry for the large
>     post, but here's my .mdp and .top
> 
>     ---------------------------------------------------------------------------------
>     title = Conjugated-Gradients Energy Minimization
> 
>     ; Preprocessing
>     cpp = /lib/cpp
>     include = -I../top
> 
>     ; Parameters
>     integrator = cg
>     emtol = 1.0
>     nsteps = 5000
>     nstenergy = 100
>     nstxtcout = 100
>     xtc_grps = System
>     energygrps = System
> 
>     ; Neighboursearching
>     nstlist = 5
>     ns_type = grid
>     rlist = 0.9
>     coulombtype = PME
>     rcoulomb = 0.9
>     rvdw = 0.9
>     constraints = none
>     pbc = xyz
>     -----------------------------------------------------------------------------------------------------
>     ; Topology from .mol2 file
>     ; topolbuild version 1.3
>     ; Command line:
>     ;     topolbuild -n BMIM_Test3 -ff amber99 -dir $AMBERHOME
>     ;
>     ; The force field files to be included
>     #include "amber99.ff/forcefield.itp"
> 
>      [ moleculetype ]
>     ; name  nrexcl
>     BMIM   3
> 
>      [ atoms ]
>     ;  nr    type   resnr   residu   atom   cgnr    charge      mass
>         1          CT     1      BMIM       C    1  -0.17243  12.01100  
>         ;  -0.1724300
>         2          HC     1      BMIM       H    1   0.07139   1.00800  
>         ;  -0.1010400
>         3          HC     1      BMIM       H    1   0.05434   1.00800  
>         ;  -0.0467000
>         4          HC     1      BMIM       H    1   0.05138   1.00800  
>         ;   0.0046800
>         5          CT     1      BMIM       C    2   0.02353  12.01100  
>         ;   0.0282100
>         6          HC     1      BMIM       H    2   0.02417   1.00800  
>         ;   0.0523800
>         7          HC     1      BMIM       H    2   0.02427   1.00800  
>         ;   0.0766500
>         8          CT     1      BMIM       C    3   0.01014  12.01100  
>         ;   0.0867900
>         9          HC     1      BMIM       H    3   0.03312   1.00800  
>         ;   0.1199100
>        10          HC     1      BMIM       H    3   0.02952   1.00800  
>         ;   0.1494300
>        11          NA     1      BMIM       N    4   0.05435  14.00670  
>         ;   0.2037800
>        12          CC     1      BMIM       C    4  -0.17380  12.01100  
>         ;   0.0299800
>        13          H4     1      BMIM       H    4   0.23709   1.00800  
>         ;   0.2670700
>        14          NA     1      BMIM       N    6   0.07275  14.00670  
>         ;   0.3398200
>        15          CT     1      BMIM       C    5  -0.07367  12.01100  
>         ;   0.2661500
>        16          H1     1      BMIM       H    5   0.10657   1.00800  
>         ;   0.3727200
>        17          H1     1      BMIM       H    5   0.09108   1.00800  
>         ;   0.4638000
>        18          CC     1      BMIM       C    6  -0.08433  12.01100  
>         ;   0.3794700
>        19          H4     1      BMIM       H    6   0.20500   1.00800  
>         ;   0.5844700
>        20          CR     1      BMIM       C    7  -0.02624  12.01100  
>         ;   0.5582300
>        21          H5     1      BMIM       H    7   0.21862   1.00800  
>         ;   0.7768500
>        22          CT     1      BMIM       C    8  -0.18403  12.01100  
>         ;   0.5928200
>        23          H1     1      BMIM       H    8   0.13823   1.00800  
>         ;   0.7310500
>        24          H1     1      BMIM       H    8   0.13886   1.00800  
>         ;   0.8699100
>        25          H1     1      BMIM       H    8   0.13010   1.00800  
>         ;   1.0000100
>     ; total molecule charge =   1.0000100
> 
>      [ bonds ]
>     ;   ai  aj   funct      b0          kb
>            1     5   1     0.15260      259408.       ;     C-     C
>            1     2   1     0.10900      284512.       ;     C-     H
>            1     3   1     0.10900      284512.       ;     C-     H
>            1     4   1     0.10900      284512.       ;     C-     H
>            5     8   1     0.15260      259408.       ;     C-     C
>            5     6   1     0.10900      284511.       ;     C-     H
>            5     7   1     0.10900      284512.       ;     C-     H
>            8    15   1     0.15260      259408.       ;     C-     C
>            8     9   1     0.10900      284512.       ;     C-     H
>            8    10   1     0.10900      284512.       ;     C-     H
>           11    12   1     0.13850      353129.       ;     N-     C
>           11    15   1     0.13850      353129.       ;     N-     C
>           11    20   1     0.13850      353129.       ;     N-     C
>           12    18   1     0.14000      392459.       ;     C-     C
>           12    13   1     0.10800      307106.       ;     C-     H
>           14    18   1     0.13850      353129.       ;     N-     C
>           14    20   1     0.13850      353129.       ;     N-     C
>           14    22   1     0.13850      353129.       ;     N-     C
>           15    16   1     0.10900      284512.       ;     C-     H
>           15    17   1     0.10900      284512.       ;     C-     H
>           18    19   1     0.10800      307106.       ;     C-     H
>           20    21   1     0.10800      307106.       ;     C-     H
>           22    23   1     0.10900      284512.       ;     C-     H
>           22    24   1     0.10900      284512.       ;     C-     H
>           22    25   1     0.10900      284512.       ;     C-     H
> 
>      [ pairs ]
>            2       8  1       ;     H-     C
>            2       6  1       ;     H-     H
>            2       7  1       ;     H-     H
>            3       8  1       ;     H-     C
>            3       6  1       ;     H-     H
>            3       7  1       ;     H-     H
>            4       8  1       ;     H-     C
>            4       6  1       ;     H-     H
>            4       7  1       ;     H-     H
>            1      15  1       ;     C-     C
>            1       9  1       ;     C-     H
>            1      10  1       ;     C-     H
>            6      15  1       ;     H-     C
>            6       9  1       ;     H-     H
>            6      10  1       ;     H-     H
>            7      15  1       ;     H-     C
>            7       9  1       ;     H-     H
>            7      10  1       ;     H-     H
>            5      11  1       ;     C-     N
>            5      16  1       ;     C-     H
>            5      17  1       ;     C-     H
>            9      11  1       ;     H-     N
>            9      16  1       ;     H-     H
>            9      17  1       ;     H-     H
>           10      11  1       ;     H-     N
>           10      16  1       ;     H-     H
>           10      17  1       ;     H-     H
>           15      18  1       ;     C-     C
>           15      13  1       ;     C-     H
>           20      13  1       ;     C-     H
>           12       8  1       ;     C-     C
>           12      16  1       ;     C-     H
>           12      17  1       ;     C-     H
>           20       8  1       ;     C-     C
>           20      16  1       ;     C-     H
>           20      17  1       ;     C-     H
>           12      21  1       ;     C-     H
>           15      14  1       ;     C-     N
>           15      21  1       ;     C-     H
>           11      19  1       ;     N-     H
>           13      14  1       ;     H-     N
>           13      19  1       ;     H-     H
>           20      19  1       ;     C-     H
>           22      12  1       ;     C-     C
>           22      19  1       ;     C-     H
>           18      21  1       ;     C-     H
>           22      11  1       ;     C-     N
>           22      21  1       ;     C-     H
>           18      23  1       ;     C-     H
>           18      24  1       ;     C-     H
>           18      25  1       ;     C-     H
>           20      23  1       ;     C-     H
>           20      24  1       ;     C-     H
>           20      25  1       ;     C-     H
> 
>     [ angles ]
>     ; ai  aj  ak  funct      th0         cth
>          2     1     5   1     109.500    418.4000     ;     H-     C-     C
>          3     1     5   1     109.500    418.4000     ;     H-     C-     C
>          4     1     5   1     109.500    418.4000     ;     H-     C-     C
>          1     5     8   1     109.500    334.7200     ;     C-     C-     C
>          1     5     6   1     109.500    418.4000     ;     C-     C-     H
>          1     5     7   1     109.500    418.4000     ;     C-     C-     H
>          3     1     2   1     109.500    292.8800     ;     H-     C-     H
>          4     1     2   1     109.500    292.8800     ;     H-     C-     H
>          4     1     3   1     109.500    292.8800     ;     H-     C-     H
>          6     5     8   1     109.500    418.4000     ;     H-     C-     C
>          7     5     8   1     109.500    418.4000     ;     H-     C-     C
>          5     8    15   1     109.500    334.7200     ;     C-     C-     C
>          5     8     9   1     109.500    418.4000     ;     C-     C-     H
>          5     8    10   1     109.500    418.4000     ;     C-     C-     H
>          7     5     6   1     109.500    292.8800     ;     H-     C-     H
>          9     8    15   1     109.500    418.4000     ;     H-     C-     C
>         10     8    15   1     109.500    418.4000     ;     H-     C-     C
>          8    15    11   1     109.500    418.4000     ;     C-     C-     N
>          8    15    16   1     109.500    418.4000     ;     C-     C-     H
>          8    15    17   1     109.500    418.4000     ;     C-     C-     H
>         10     8     9   1     109.500    292.8800     ;     H-     C-     H
>         15    11    12   1     126.400    585.7600     ;     C-     N-     C
>         20    11    12   1     120.000    585.7600     ;     C-     N-     C
>         11    12    18   1     126.400    585.7600     ;     N-     C-     C
>         11    12    13   1     120.000    418.4000     ;     N-     C-     H
>         20    11    15   1     126.400    585.7600     ;     C-     N-     C
>         11    15    16   1     109.500    418.4000     ;     N-     C-     H
>         11    15    17   1     109.500    418.4000     ;     N-     C-     H
>         11    20    14   1     120.000    585.7600     ;     N-     C-     N
>         11    20    21   1     120.000    418.4000     ;     N-     C-     H
>         13    12    18   1     120.000    418.4000     ;     H-     C-     C
>         12    18    14   1     126.400    585.7600     ;     C-     C-     N
>         12    18    19   1     120.000    418.4000     ;     C-     C-     H
>         20    14    18   1     120.000    585.7600     ;     C-     N-     C
>         22    14    18   1     126.400    585.7600     ;     C-     N-     C
>         14    18    19   1     120.000    418.4000     ;     N-     C-     H
>         22    14    20   1     120.000    585.7600     ;     C-     N-     C
>         14    20    21   1     120.000    418.4000     ;     N-     C-     H
>         14    22    23   1     109.500    418.4000     ;     N-     C-     H
>         14    22    24   1     109.500    418.4000     ;     N-     C-     H
>         14    22    25   1     109.500    418.4000     ;     N-     C-     H
>         17    15    16   1     109.500    292.8800     ;     H-     C-     H
>         24    22    23   1     109.500    292.8800     ;     H-     C-     H
>         25    22    23   1     109.500    292.8800     ;     H-     C-     H
>         25    22    24   1     109.500    292.8800     ;     H-     C-     H
> 
>     [ dihedrals ]
>     ; ai  aj   ak  al          funct    phi0          cp      mult
>          2     1     5     8   1       0.000       0.669         3  ;
>     dih      H-     C-     C-     C
>          1     5     8    15   1       0.000       0.753         3  ;
>     dih      C-     C-     C-     C
>          1     5     8    15   1     180.000       1.046         2  ;
>     dih      C-     C-     C-     C
>          1     5     8    15   1     180.000       0.837         1  ;
>     dih      C-     C-     C-     C
>          5     8    15    11   1       0.000       0.651         3  ;
>     dih      C-     C-     C-     N
>         20    11    12    18   9     180.0       5.85760         2  ;
>     dih      C-     N-     C-     C
>         12    11    15     8   1       0.000       0.000         2  ;
>     dih      C-     N-     C-     C
>         12    11    20    14   9     180.0       5.85760         2  ;
>     dih      C-     N-     C-     N
>         11    12    18    14   1     180.000      15.167         2  ;
>     dih      N-     C-     C-     N
>         20    14    18    12   9     180.0       5.85760         2  ;
>     dih      C-     N-     C-     C
>         18    14    20    11   9     180.0       5.85760         2  ;
>     dih      C-     N-     C-     N
>         18    14    22    23   1       0.000       0.000         2  ;
>     dih      C-     N-     C-     H
>         11    12    15    20   1     180.000       4.602         2  ;
>     imp      N-     C-     C-     C
>         12    11    18    13   1     180.000       4.602         2  ;
>     imp      C-     N-     C-     H
>         14    18    20    22   1     180.000       4.602         2  ;
>     imp      N-     C-     C-     C
>         18    12    14    19   1     180.000       4.602         2  ;
>     imp      C-     C-     N-     H
>         20    11    14    21   1     180.000       4.602         2  ;
>     imp      C-     N-     N-     H
> 
>     ; Include Position restraint file
>     ; WARNING: Position restraints and distance restraints ought not be
>     done together
>     #ifdef POSRES
>     #include "posreBMIM_Test3.itp"
>     #endif
> 
>     ; Include TFO topology
>     #include TFO_amber99.itp
> 
>     ; Include water topology
>     #include "tip3p.itp"
> 
>     #ifdef POSRES_WATER
>     ; Position restraint for each water oxygen
>     [ position_restraints ]
>     ;  i funct       fcx        fcy        fcz
>        1    1       1000       1000       1000
>     #endif
> 
>     ; Include generic topology for ions
>     #include "ions.itp"
> 
>      [ system ]
>     ; title from mol2 input
>     BMIMTFO
> 
>      [ molecules ]
>     ; molecule name    nr.
>     BMIM           1
>     TFO 1
>     --------------------------------------------------------------------------------------------
>     Any thought, advice and support is greatly appreciated. Thank you.
> 
>     -Alif-
> 
>     --
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> 
> 
> 
> -- 
> ORNL/UT Center for Molecular Biophysics cmb.ornl.gov <http://cmb.ornl.gov>
> 865-241-1537, ORNL PO BOX 2008 MS6309
> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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