[gmx-users] %exist hydrogen bond - difference between number of lines in input and output files

Justin A. Lemkul jalemkul at vt.edu
Thu Feb 24 15:01:58 CET 2011



leila karami wrote:
> Dear Justin
> 
> I have a question about output file (summary_HBmap.dat).
> 
> my input file (hbound.ndx) is as follows:
> 
> [ hbonds_Protein-ab_interfacial ]
>    1019   1021  13088
>    1019   1021  23621
>    1016   1018  23621
>    1013   1014  12392
>    1013   1014  23621
>     992    994  12392
>     946    949  23621
>     853    855   6251
>     853    855  15473
>     777    780  15473
>     777    780  23621
>     723    725  15473
>     720    722  15473
>     504    506  23621
>     501    503  23621
>      98    100   7952
>      98    100  15473
>      92     93   7952
>      79     80   7952
>      58     60  15473
>      46     47   7952
>      46     47  15473
>      39     40  18725
>    6251   6252    796
>    6251   6252    866
>    6251   6252    867
>    7952   7953     62
>   12392  12393    857
>   12392  12393    919
>   12392  12393    920
>   13088  13089    857
>   13088  13089    858
>   13088  13089    866
>   15473  15474     57
>   15473  15474     62
>   15473  15474    701
>   15473  15474    703
>   18725  18726     45
>   18725  18726     62
>   18725  18726     63
>   19556  19557    744
>   19556  19557    797
>   23621  23622    866
>   23621  23622    871
>   23621  23622   1041
> 
> It contains 45 lines showing hydrogen bonds.
> 
> my out put file is as follows:
> 
> #    Donor                    Acceptor                % Exist.
>                                                              0.400
>      ARG58           NH2       SOL3827            OW        43.565
>      ARG58           NH2       SOL7338            OW        21.663
>      ARG58           NH1       SOL7338            OW         0.160
>      ARG58            NE       SOL3595            OW         1.359
>      ARG58            NE       SOL7338            OW        59.233
>      ARG57           NH1       SOL3595            OW         0.480
>      LYP55            NZ       SOL7338            OW        98.002
>      GLN50           NE2       SOL1548            OW         1.359
>      GLN50           NE2       SOL4622            OW         0.080
>      LYP46            NZ       SOL4622            OW         0.480
>      LYP46            NZ       SOL7338            OW         0.320
>      ARG43           NH2       SOL4622            OW         0.320
>      ARG43           NH1       SOL4622            OW         0.160
>      ARG31           NH2       SOL7338            OW         0.240
>      ARG31           NH1       SOL7338            OW        16.147
>       ARG7           NH2       SOL2115            OW         3.277
>       ARG7           NH2       SOL4622            OW        50.679
>       ARG7            NE       SOL2115            OW         0.320
>       ARG7             N       SOL2115            OW         0.719
>       GLN5           NE2       SOL4622            OW         0.320
>       GLN5             N       SOL2115            OW        32.534
>       GLN5             N       SOL4622            OW        78.497
>       GLY4             N       SOL5706            OW         0.400
>    SOL1548            OW         THR47             O        61.711
>    SOL1548            OW         ASN51           OD1        16.867
>    SOL1548            OW         ASN51           ND2        95.044
>    SOL2115            OW          GLN5             O        16.147
>    SOL3595            OW         GLN50             O         0.080
>    SOL3595            OW         ARG53             O         0.080
>    SOL3595            OW         ALA54             N         3.118
>    SOL3827            OW         GLN50             O         0.080
>    SOL3827            OW         ASN51             N        33.253
>    SOL3827            OW         ASN51           OD1         0.560
>    SOL4622            OW          GLN5           OE1         1.279
>    SOL4622            OW          GLN5             O         0.080
>    SOL4622            OW         GLU42           OE2         0.080
>    SOL4622            OW         GLU42             O         2.798
>    SOL5706            OW          GLY4             O         0.080
>    SOL5706            OW          GLN5             O         0.080
>    SOL5706            OW          VAL6             N        96.003
>    SOL5983            OW         GLN44             O         0.160
>    SOL5983            OW         TRP48             N         0.160
>    SOL7338            OW         ASN51           OD1         0.320
>    SOL7338            OW         ASN51             O         0.080
> 
> It contains 44 lines showing hydrogen bonds and 45 lines showing % exist.
> 
> I want to know reason this difference in number of lines.
> 
> and also I want to know what is 0.400 in second line of output file?
> 
> at the end of input file, there is 3 lines
> 23621  23622    866
> 23621  23622    871
> 23621  23622   1041
> corresponding to SOL7338, but at the end of output file, there is 2 lines
> SOL7338            OW         ASN51           OD1         0.320
> SOL7338            OW         ASN51             O         0.080
> 
> please explain this differences more.
> 

Your .ndx file should not contain the directive heading.  Per the instructions 
in the header of the .pl script: "bond.ndx (modified to contain only the atom 
numbers in the [hbonds...] section, nothing else)"

-Justin

> best regards.
> 
> 
> 
> 
> 
> -- 
> 
> Leila Karami
> Ph.D. student of Physical Chemistry
> K.N. Toosi University of Technology
> 
> Theoretical Physical Chemistry Group
> 
> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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