[gmx-users] Iusses while converting a POPE pdb with pdb2gmx and ff charmm27

Justin A. Lemkul jalemkul at vt.edu
Wed Jun 29 13:29:15 CEST 2011



Leonhard Henkes wrote:
> Thanks for your reply,
> 
> my whole pdb file includes an equilibrated membrane bilayer, constructed with VMD for NAMD. I used the CHARMM27 FF while constructing it.
> Now, I wanted to convert this system for a run in Gromacs. 
> To solve my issus, I divided my system into four new pdb's (ion, water, lipids, protein). This was an advise I found in an older thread. In the end, all created *.gro files will be united again, to one big file.
> 

The only species you have to explicitly deal with are the protein and lipids. 
The water and ions do not need to be processed by pdb2gmx.  Their topologies are 
handled by #include statements later.

> I checked the POPE entry in the charmff folder and the lipid names are the same as in my pdb. So I assumed that my bilayer can be converted with pdb2gmx too. The occurring errors are attached. 
> 
> I don't understand these errors.

The combination of long bond warnings and mismatching names suggests that the 
.pdb file is misformatted.  Gromacs requires that the exact PDB standard be 
upheld, and a quick look at your file indicates that at the very least, your 
coordinate columns are shifted left, which leads to incorrect interpretation of 
the values and gives the long bond warnings.  This may also have a side effect 
related to the atom names.  Start over with a correctly formatted .pdb file.

-Justin

> Thanks for your help,
> 
> M.Kalavera
> 
> ...
> Warning: Long Bond (232-234 = 0.547183 nm)
> Warning: Long Bond (232-235 = 0.710465 nm)
> Warning: Long Bond (235-236 = 0.514028 nm)
> Warning: Long Bond (235-237 = 0.707876 nm)
> Warning: Long Bond (235-238 = 0.341151 nm)
> Warning: Long Bond (238-239 = 0.545028 nm)
> Warning: Long Bond (238-240 = 0.538926 nm)
> Warning: Long Bond (241-242 = 0.586707 nm)
> Warning: Long Bond (241-243 = 0.360561 nm)
> Warning: Long Bond (241-244 = 0.447081 nm)
> Warning: Long Bond (244-245 = 0.57 nm)
> Warning: Long Bond (244-246 = 0.412897 nm)
> Warning: Long Bond (244-247 = 0.414759 nm)
> Warning: Long Bond (247-248 = 0.785856 nm)
> Warning: Long Bond (247-249 = 0.865684 nm)
> Warning: Long Bond (247-250 = 0.447969 nm)
> 
> WARNING: atom P is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom O13 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom O14 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom O11 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom O12 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom C1 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom HA is missing in residue POPE 1 in the pdb file
>          You might need to add atom HA to the hydrogen database of building block POPE
>          in the file lipids.hdb (see the manual)
> 
> 
> WARNING: atom HB is missing in residue POPE 1 in the pdb file
>          You might need to add atom HB to the hydrogen database of building block POPE
>          in the file lipids.hdb (see the manual)
> 
> 
> WARNING: atom C2 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom HS is missing in residue POPE 1 in the pdb file
>          You might need to add atom HS to the hydrogen database of building block POPE
>          in the file lipids.hdb (see the manual)
> 
> 
> WARNING: atom O21 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom C21 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom O22 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom C22 is missing in residue POPE 1 in the pdb file
> 
> 
> WARNING: atom H2R is missing in residue POPE 1 in the pdb file
>          You might need to add atom H2R to the hydrogen database of building block POPE
>          in the file lipids.hdb (see the manual)
> 
> 
> WARNING: atom H2S is missing in residue POPE 1 in the pdb file
>          You might need to add atom H2S to the hydrogen database of building block POPE
>          in the file lipids.hdb (see the manual)
> 
> 
> WARNING: atom C3 is missing in residue POPE 1 in the pdb file
> 
> ...
> 
> -------------------------------------------------------
> Program pdb2gmx_mpi, VERSION 4.5.4
> Source code file: /usr/people/../gromacs-4.5.4/src/kernel/pdb2top.c, line: 1463
> 
> Fatal error:
> There were 250 missing atoms in molecule Lipids, if you want to use this incomplete topology anyhow, use the option -missing
> For more information and tips for troubleshooting, please check the GROMACS
> website at http://www.gromacs.org/Documentation/Errors
> -------------------------------------------------------
> 
> 
> 
>>
>> Kalavera at gmx.net wrote:
>>> Dear gromacs users,
>>>
>>> I constructed a small *.pdb file comprised of only lipids (POPE
>> bilayer). If I try to convert this pdb file with pdb2gmx (gromacs 4.5.4) and the
>> included charmm27 forcefield, I get warnings for "missing hydrogen atoms" and
>> "Long Bond" warnings.  
>> pdb2gmx is ill-suited for dealing with multiple molecules.  The better
>> approach 
>> is to work with a single POPE molecule with a suitably constructed .rtp
>> entry 
>> for it.  pdb2gmx should then generate a topology for one lipid, which can
>> then 
>> be modified to be used in an #include statement or extended to accommodate
>> an 
>> entire bilayer simply by altering the [molecules] directive.
>>
>> The errors you are seeing are likely due to naming mismatch or poor
>> starting 
>> geometry, but since you haven't quoted the errors, I don't dare guess
>> beyond that.
>>
>>> In other threads, the lipids.hdb is discussed in this context. In my
>> case, all hydrogen atoms are available. Do I need to proceed the lipids.hdb
>> (like described in the manual), or can I leave it empty ?  
>>
>> The .hdb file is only necessary to reconstruct missing H atoms.  If your 
>> structure has all of them and they are correctly named, then an .hdb is
>> not 
>> necessary.
>>
>> -Justin
>>
>>> The resnames are consistent with the lipids.rtp entries.
>>>
>>> Thanks for all your help,
>>>
>>> M.Kalavera
>>>
>>>
>>> --------------------Additional
>> information--------------------------------
>>> Command used:
>>>
>>> pdb2gmx_mpi -f lipids.pdb -p lipids.top -o lipids.gro -water TIP3P -ff
>> charmm27
>>> The first entries of my pdb file:
>>>
>>> REMARK
>>> ATOM      1  N   POPE    1	-14.135 -21.952  22.035  1.00  0.00	 LIPI   
>>> ATOM      2  HN1 POPE    1	-14.125 -22.660  22.797  1.00  0.00	 LIPI   
>>> ATOM      3  HN2 POPE    1	-13.175 -21.821  21.659  1.00  0.00	 LIPI   
>>> ATOM      4  HN3 POPE    1	-14.339 -21.041  22.493  1.00  0.00	 LIPI   
>>> ATOM      5  C12 POPE    1	-15.154 -22.312  21.033  1.00  0.00	 LIPI   
>>> ATOM      6 H12A POPE    1	-16.107 -22.486  21.577  1.00  0.00	 LIPI   
>>> ATOM      7 H12B POPE    1	-14.920 -23.252  20.489  1.00  0.00	 LIPI   
>>> ATOM      8  C11 POPE    1	-15.234 -21.168  20.058  1.00  0.00	 LIPI   
>>> ATOM      9 H11A POPE    1	-14.276 -20.976  19.529  1.00  0.00	 LIPI   
>>> ATOM     10 H11B POPE    1	-15.909 -21.556  19.266  1.00  0.00	 LIPI   
>>> ATOM     11  P   POPE    1	-15.310 -18.486  20.024  1.00  0.00	 LIPI   
>>> ATOM     12  O13 POPE    1	-16.549 -17.712  19.663  1.00  0.00	 LIPI   
>>> ATOM     13  O14 POPE    1	-14.314 -17.846  20.940  1.00  0.00	 LIPI   
>>> ATOM     14  O11 POPE    1	-14.637 -18.744  18.604  1.00  0.00	 LIPI   
>>> ATOM     15  O12 POPE    1	-15.752 -19.947  20.557  1.00  0.00	 LIPI   
>>> ATOM     16  C1  POPE    1	-14.267 -17.491  18.032  1.00  0.00	 LIPI   
>>> ATOM     17  HA  POPE    1	-15.110 -16.900  17.614  1.00  0.00	 LIPI   
>>> ATOM     18  HB  POPE    1	-13.690 -16.851  18.733  1.00  0.00	 LIPI   
>>> ATOM     19  C2  POPE    1	-13.395 -17.725  16.681  1.00  0.00	 LIPI   
>>> ATOM     20  HS  POPE    1	-12.357 -17.976  16.987  1.00  0.00	 LIPI   
>>> ATOM     21  O21 POPE    1	-13.383 -16.549  15.849  1.00  0.00	 LIPI   
>>> ATOM     22  C21 POPE    1	-12.419 -16.414  14.956  1.00  0.00	 LIPI   
>>> ATOM     23  O22 POPE    1	-11.405 -17.098  14.835  1.00  0.00	 LIPI   
>>> ATOM     24  C22 POPE    1	-12.767 -15.262  13.918  1.00  0.00	 LIPI   
>>> ATOM     25  H2R POPE    1	-13.659 -15.556  13.324  1.00  0.00	 LIPI   
>>> ATOM     26  H2S POPE    1	-12.825 -14.275  14.427  1.00  0.00	 LIPI   
>>> ATOM     27  C3  POPE    1	-13.924 -18.925  15.834  1.00  0.00	 LIPI   
>>> ATOM     28  HX  POPE    1	-13.196 -19.033  15.002  1.00  0.00	 LIPI   
>>> ATOM     29  HY  POPE    1	-13.829 -19.915  16.331  1.00  0.00	 LIPI   
>>> ATOM     30  O31 POPE    1	-15.338 -18.716  15.377  1.00  0.00	 LIPI   
>>> ATOM     31  C31 POPE    1	-15.514 -19.308  14.227  1.00  0.00	 LIPI   
>>> ATOM     32  O32 POPE    1	-14.683 -19.825  13.544  1.00  0.00	 LIPI   
>>> ATOM     33  C32 POPE    1	-17.023 -19.166  13.689  1.00  0.00	 LIPI   
>>> ATOM     34  H2X POPE    1	-17.106 -20.050  13.022  1.00  0.00	 LIPI   
>>> ATOM     35  H2Y POPE    1	-17.627 -19.380  14.597  1.00  0.00	 LIPI   
>>> ATOM     36  C23 POPE    1	-11.614 -14.871  12.965  1.00  0.00	 LIPI   
>>> ATOM     37  H3R POPE    1	-11.533 -13.767  12.868  1.00  0.00	 LIPI   
>>> ATOM     38  H3S POPE    1	-10.669 -15.218  13.434  1.00  0.00	 LIPI   
>>> ATOM     39  C24 POPE    1	-11.678 -15.385  11.524  1.00  0.00	 LIPI   
>>> ATOM     40  H4R POPE    1	-12.586 -14.837  11.192  1.00  0.00	 LIPI   
>>> ATOM     41  H4S POPE    1	-10.819 -15.050  10.904  1.00  0.00	 LIPI   
>>> ATOM     42  C25 POPE    1	-12.001 -16.882  11.443  1.00  0.00	 LIPI   
>>> ATOM     43  H5R POPE    1	-11.079 -17.466  11.651  1.00  0.00	 LIPI   
>>> ATOM     44  H5S POPE    1	-12.770 -17.181  12.187  1.00  0.00	 LIPI   
>>> ATOM     45  C26 POPE    1	-12.435 -17.332  10.094  1.00  0.00	 LIPI   
>>> ATOM     46  H6R POPE    1	-12.808 -18.375  10.172  1.00  0.00	 LIPI   
>>> ATOM     47  H6S POPE    1	-13.247 -16.723   9.640  1.00  0.00	 LIPI   
>>> ATOM     48  C27 POPE    1	-11.179 -17.198   9.148  1.00  0.00	 LIPI   
>>> ATOM     49  H7R POPE    1	-10.668 -16.220   9.280  1.00  0.00	 LIPI   
>>> ATOM     50  H7S POPE    1	-10.407 -17.957   9.395  1.00  0.00	 LIPI   
>>> ATOM     51  C28 POPE    1	-11.573 -17.384   7.605  1.00  0.00	 LIPI   
>>> ATOM     52  H8R POPE    1	-12.256 -16.556   7.320  1.00  0.00	 LIPI   
>>> ATOM     53  H8S POPE    1	-10.612 -17.358   7.047  1.00  0.00	 LIPI   
>>> ATOM     54  C29 POPE    1	-12.262 -18.679   7.532  1.00  0.00	 LIPI   
>>> ATOM     55  H91 POPE    1	-11.685 -19.498   7.987  1.00  0.00	 LIPI   
>>> ATOM     56 C210 POPE    1	-13.523 -18.906   7.069  1.00  0.00	 LIPI   
>>> ATOM     57 H101 POPE    1	-14.026 -19.883   7.028  1.00  0.00	 LIPI   
>>> ATOM     58 C211 POPE    1	-14.472 -17.934   6.435  1.00  0.00	 LIPI   
>>> ATOM     59 H11R POPE    1	-15.424 -18.007   7.003  1.00  0.00	 LIPI   
>>> ATOM     60 H11S POPE    1	-14.170 -16.865   6.447  1.00  0.00	 LIPI   
>>> ATOM     61 C212 POPE    1	-14.750 -18.261   4.918  1.00  0.00	 LIPI   
>>> ATOM     62 H12R POPE    1	-13.797 -18.179   4.352  1.00  0.00	 LIPI   
>>> ATOM     63 H12S POPE    1	-15.149 -19.289   4.785  1.00  0.00	 LIPI   
>>> ATOM     64 C213 POPE    1	-15.756 -17.364   4.264  1.00  0.00	 LIPI   
>>> ATOM     65 H13R POPE    1	-16.769 -17.520   4.691  1.00  0.00	 LIPI   
>>> ATOM     66 H13S POPE    1	-15.470 -16.366   4.662  1.00  0.00	 LIPI   
>>> ATOM     67 C214 POPE    1	-15.787 -17.415   2.718  1.00  0.00	 LIPI   
>>> ATOM     68 H14R POPE    1	-14.803 -16.993   2.420  1.00  0.00	 LIPI   
>>> ATOM     69 H14S POPE    1	-15.977 -18.434   2.318  1.00  0.00	 LIPI   
>>> ATOM     70 C215 POPE    1	-16.823 -16.519   2.048  1.00  0.00	 LIPI   
>>> ATOM     71 H15R POPE    1	-17.829 -16.852   2.381  1.00  0.00	 LIPI   
>>> ATOM     72 H15S POPE    1	-16.676 -15.433   2.229  1.00  0.00	 LIPI   
>>> ATOM     73 C216 POPE    1	-16.797 -16.645   0.489  1.00  0.00	 LIPI   
>>> ATOM     74 H16R POPE    1	-15.771 -16.468   0.101  1.00  0.00	 LIPI   
>>> ATOM     75 H16S POPE    1	-17.118 -17.640   0.113  1.00  0.00	 LIPI   
>>> ATOM     76 C217 POPE    1	-17.792 -15.697  -0.156  1.00  0.00	 LIPI   
>>> ATOM     77 H17R POPE    1	-18.763 -16.230  -0.066  1.00  0.00	 LIPI   
>>> ATOM     78 H17S POPE    1	-17.746 -14.662   0.244  1.00  0.00	 LIPI   
>>> ATOM     79 C218 POPE    1	-17.473 -15.721  -1.669  1.00  0.00	 LIPI   
>>> ATOM     80 H18R POPE    1	-16.591 -15.052  -1.774  1.00  0.00	 LIPI   
>>> ATOM     81 H18S POPE    1	-17.038 -16.672  -2.044  1.00  0.00	 LIPI   
>>> ATOM     82 H18T POPE    1	-18.351 -15.355  -2.244  1.00  0.00	 LIPI   
>>> ATOM     83  C33 POPE    1	-17.263 -17.863  13.021  1.00  0.00	 LIPI   
>>> ATOM     84  H3X POPE    1	-18.337 -17.753  12.759  1.00  0.00	 LIPI   
>>> ATOM     85  H3Y POPE    1	-17.135 -16.996  13.704  1.00  0.00	 LIPI   
>>> ATOM     86  C34 POPE    1	-16.410 -17.562  11.765  1.00  0.00	 LIPI   
>>> ATOM     87  H4X POPE    1	-15.354 -17.327  12.018  1.00  0.00	 LIPI   
>>> ATOM     88  H4Y POPE    1	-16.387 -18.484  11.146  1.00  0.00	 LIPI   
>>> ATOM     89  C35 POPE    1	-16.833 -16.334  10.951  1.00  0.00	 LIPI   
>>> ATOM     90  H5X POPE    1	-17.925 -16.159  11.065  1.00  0.00	 LIPI   
>>> ATOM     91  H5Y POPE    1	-16.256 -15.483  11.371  1.00  0.00	 LIPI   
>>> ATOM     92  C36 POPE    1	-16.606 -16.326   9.426  1.00  0.00	 LIPI   
>>> ATOM     93  H6X POPE    1	-15.530 -16.353   9.151  1.00  0.00	 LIPI   
>>> ATOM     94  H6Y POPE    1	-17.037 -17.279   9.051  1.00  0.00	 LIPI   
>>> ATOM     95  C37 POPE    1	-17.335 -15.184   8.706  1.00  0.00	 LIPI   
>>> ATOM     96  H7X POPE    1	-18.428 -15.164   8.900  1.00  0.00	 LIPI   
>>> ATOM     97  H7Y POPE    1	-16.893 -14.303   9.217  1.00  0.00	 LIPI   
>>> ATOM     98  C38 POPE    1	-17.011 -15.122   7.240  1.00  0.00	 LIPI   
>>> ATOM     99  H8X POPE    1	-15.921 -14.914   7.192  1.00  0.00	 LIPI   
>>> ATOM    100  H8Y POPE    1	-17.159 -16.168   6.893  1.00  0.00	 LIPI   
>>> ATOM    101  C39 POPE    1	-17.912 -14.117   6.532  1.00  0.00	 LIPI   
>>> ATOM    102  H9X POPE    1	-18.916 -14.581   6.429  1.00  0.00	 LIPI   
>>> ATOM    103  H9Y POPE    1	-18.095 -13.292   7.254  1.00  0.00	 LIPI   
>>> ATOM    104 C310 POPE    1	-17.433 -13.541   5.200  1.00  0.00	 LIPI   
>>> ATOM    105 H10X POPE    1	-16.509 -12.923   5.196  1.00  0.00	 LIPI   
>>> ATOM    106 H10Y POPE    1	-17.348 -14.376   4.472  1.00  0.00	 LIPI   
>>> ATOM    107 C311 POPE    1	-18.588 -12.632   4.746  1.00  0.00	 LIPI   
>>> ATOM    108 H11X POPE    1	-19.579 -13.093   4.550  1.00  0.00	 LIPI   
>>> ATOM    109 H11Y POPE    1	-18.678 -11.887   5.565  1.00  0.00	 LIPI   
>>> ATOM    110 C312 POPE    1	-18.220 -11.803   3.580  1.00  0.00	 LIPI   
>>> ATOM    111 H12X POPE    1	-18.892 -10.923   3.487  1.00  0.00	 LIPI   
>>> ATOM    112 H12Y POPE    1	-17.220 -11.363   3.783  1.00  0.00	 LIPI   
>>> ATOM    113 C313 POPE    1	-18.225 -12.507   2.203  1.00  0.00	 LIPI   
>>> ATOM    114 H13X POPE    1	-17.509 -13.356   2.240  1.00  0.00	 LIPI   
>>> ATOM    115 H13Y POPE    1	-19.240 -12.936   2.061  1.00  0.00	 LIPI   
>>> ATOM    116 C314 POPE    1	-17.884 -11.584   1.011  1.00  0.00	 LIPI   
>>> ATOM    117 H14X POPE    1	-16.839 -11.206   1.013  1.00  0.00	 LIPI   
>>> ATOM    118 H14Y POPE    1	-18.021 -12.224   0.112  1.00  0.00	 LIPI   
>>> ATOM    119 C315 POPE    1	-18.727 -10.322   0.930  1.00  0.00	 LIPI   
>>> ATOM    120 H15X POPE    1	-19.752 -10.654   1.201  1.00  0.00	 LIPI   
>>> ATOM    121 H15Y POPE    1	-18.404  -9.470   1.565  1.00  0.00	 LIPI   
>>> ATOM    122 C316 POPE    1	-18.693  -9.699  -0.511  1.00  0.00	 LIPI   
>>> ATOM    123 H16X POPE    1	-18.869 -10.363  -1.384  1.00  0.00	 LIPI   
>>> ATOM    124 H16Y POPE    1	-19.343  -8.799  -0.554  1.00  0.00	 LIPI   
>>> ATOM    125 H16Z POPE    1	-17.667  -9.344  -0.746  1.00  0.00	 LIPI   
>>> ATOM    126  N   POPE    2     -16.581 -10.534  24.356  1.00  0.00	 11  
>>> ATOM    127  HN1 POPE    2     -16.732 -11.549  24.192  1.00  0.00	 11  
>>> ATOM    128  HN2 POPE    2     -16.455 -10.448  25.385  1.00  0.00	 11  
>>> ATOM    129  HN3 POPE    2     -17.341  -9.955  23.946  1.00  0.00	 11  
>>> ATOM    130  C12 POPE    2     -15.418  -9.959  23.562  1.00  0.00	 11  
>>> ATOM    131 H12A POPE    2     -14.494 -10.249  24.107  1.00  0.00	 11  
>>> ATOM    132 H12B POPE    2     -15.513  -8.855  23.485  1.00  0.00	 11  
>>> ATOM    133  C11 POPE    2     -15.477 -10.490  22.195  1.00  0.00	 11  
>>> ATOM    134 H11A POPE    2     -15.479 -11.589  22.357  1.00  0.00	 11  
>>> ATOM    135 H11B POPE    2     -16.417 -10.221  21.669  1.00  0.00	 11  
>>> ATOM    136  P   POPE    2     -13.939 -10.574  20.043  1.00  0.00	 11  
>>> ATOM    137  O13 POPE    2     -13.116  -9.584  19.337  1.00  0.00	 11  
>>> ATOM    138  O14 POPE    2     -13.410 -11.912  20.205  1.00  0.00	 11  
>>> ATOM    139  O11 POPE    2     -15.294 -10.583  19.326  1.00  0.00	 11  
>>> ATOM    140  O12 POPE    2     -14.342 -10.006  21.451  1.00  0.00	 11  
>>> ATOM    141  C1  POPE    2     -15.777 -11.692  18.643  1.00  0.00	 11  
>>> ATOM    142  HA  POPE    2     -15.937 -12.530  19.354  1.00  0.00	 11  
>>> ATOM    143  HB  POPE    2     -16.690 -11.461  18.052  1.00  0.00	 11  
>>> ATOM    144  C2  POPE    2     -14.768 -12.202  17.559  1.00  0.00	 11  
>>> ATOM    145  HS  POPE    2     -13.781 -12.417  18.021  1.00  0.00	 11  
>>> ATOM    146  O21 POPE    2     -14.717 -11.182  16.551  1.00  0.00	 11  
>>> ATOM    147  C21 POPE    2     -13.685 -11.334  15.740  1.00  0.00	 11  
>>> ATOM    148  O22 POPE    2     -12.806 -12.164  15.795  1.00  0.00	 11  
>>> ATOM    149  C22 POPE    2     -13.765 -10.175  14.699  1.00  0.00	 11  
>>> ATOM    150  H2R POPE    2     -14.793  -9.838  14.445  1.00  0.00	 11  
>>> ATOM    151  H2S POPE    2     -13.101  -9.401  15.140  1.00  0.00	 11  
>>> ATOM    152  C3  POPE    2     -15.233 -13.539  17.004  1.00  0.00	 11  
>>> ATOM    153  HX  POPE    2     -14.585 -14.054  16.263  1.00  0.00	 11  
>>> ATOM    154  HY  POPE    2     -15.065 -14.306  17.790  1.00  0.00	 11  
>>> ATOM    155  O31 POPE    2     -16.619 -13.710  16.710  1.00  0.00	 11  
>>> ATOM    156  C31 POPE    2     -16.906 -14.067  15.472  1.00  0.00	 11  
>>> ATOM    157  O32 POPE    2     -16.145 -14.464  14.651  1.00  0.00	 11  
>>> ATOM    158  C32 POPE    2     -18.400 -14.146  15.459  1.00  0.00	 11  
>>> ATOM    159  H2X POPE    2     -18.705 -15.059  16.013  1.00  0.00	 11  
>>> ATOM    160  H2Y POPE    2     -18.797 -13.189  15.860  1.00  0.00	 11  
>>> ATOM    161  C23 POPE    2     -13.208 -10.644  13.348  1.00  0.00	 11  
>>> ATOM    162  H3R POPE    2     -13.291  -9.830  12.597  1.00  0.00	 11  
>>> ATOM    163  H3S POPE    2     -12.132 -10.897  13.467  1.00  0.00	 11  
>>> ATOM    164  C24 POPE    2     -14.021 -11.792  12.774  1.00  0.00	 11  
>>> ATOM    165  H4R POPE    2     -14.077 -12.587  13.547  1.00  0.00	 11  
>>> ATOM    166  H4S POPE    2     -15.010 -11.289  12.717  1.00  0.00	 11  
>>> ATOM    167  C25 POPE    2     -13.478 -12.157  11.411  1.00  0.00	 11  
>>> ATOM    168  H5R POPE    2     -12.391 -12.386  11.379  1.00  0.00	 11  
>>> ATOM    169  H5S POPE    2     -13.867 -13.135  11.054  1.00  0.00	 11  
>>> ATOM    170  C26 POPE    2     -13.814 -11.112  10.310  1.00  0.00	 11  
>>> ATOM    171  H6R POPE    2     -13.655 -10.079  10.688  1.00  0.00	 11  
>>> ATOM    172  H6S POPE    2     -13.090 -11.358   9.504  1.00  0.00	 11  
>>> ATOM    173  C27 POPE    2     -15.266 -10.903   9.760  1.00  0.00	 11  
>>> ATOM    174  H7R POPE    2     -15.806 -11.873   9.770  1.00  0.00	 11  
>>> ATOM    175  H7S POPE    2     -15.830 -10.494  10.624  1.00  0.00	 11  
>>> ATOM    176  C28 POPE    2     -15.386  -9.992   8.529  1.00  0.00	 11  
>>> ATOM    177  H8R POPE    2     -16.443  -9.800   8.247  1.00  0.00	 11  
>>> ATOM    178  H8S POPE    2     -14.938  -9.003   8.763  1.00  0.00	 11  
>>> ATOM    179  C29 POPE    2     -14.678 -10.633   7.334  1.00  0.00	 11  
>>> ATOM    180  H91 POPE    2     -15.190 -11.479   6.852  1.00  0.00	 11  
>>> ATOM    181 C210 POPE    2     -13.575 -10.196   6.793  1.00  0.00	 11  
>>> ATOM    182 H101 POPE    2     -13.240 -10.607   5.829  1.00  0.00	 11  
>>> ATOM    183 C211 POPE    2     -12.640  -8.980   7.199  1.00  0.00	 11  
>>> ATOM    184 H11R POPE    2     -12.868  -8.683   8.245  1.00  0.00	 11  
>>> ATOM    185 H11S POPE    2     -11.579  -9.306   7.163  1.00  0.00	 11  
>>> ATOM    186 C212 POPE    2     -12.854  -7.742   6.343  1.00  0.00	 11  
>>> ATOM    187 H12R POPE    2     -13.933  -7.479   6.386  1.00  0.00	 11  
>>> ATOM    188 H12S POPE    2     -12.174  -6.936   6.692  1.00  0.00	 11  
>>> ATOM    189 C213 POPE    2     -12.328  -7.972   4.909  1.00  0.00	 11  
>>> ATOM    190 H13R POPE    2     -11.336  -8.463   5.005  1.00  0.00	 11  
>>> ATOM    191 H13S POPE    2     -12.985  -8.717   4.410  1.00  0.00	 11  
>>> ATOM    192 C214 POPE    2     -12.262  -6.698   4.036  1.00  0.00	 11  
>>> ATOM    193 H14R POPE    2     -13.075  -6.009   4.351  1.00  0.00	 11  
>>> ATOM    194 H14S POPE    2     -11.355  -6.122   4.318  1.00  0.00	 11  
>>> ATOM    195 C215 POPE    2     -12.402  -7.080   2.551  1.00  0.00	 11  
>>> ATOM    196 H15R POPE    2     -11.731  -7.924   2.282  1.00  0.00	 11  
>>> ATOM    197 H15S POPE    2     -13.468  -7.305   2.335  1.00  0.00	 11  
>>> ATOM    198 C216 POPE    2     -12.163  -5.875   1.636  1.00  0.00	 11  
>>> ATOM    199 H16R POPE    2     -12.918  -5.077   1.804  1.00  0.00	 11  
>>> ATOM    200 H16S POPE    2     -11.147  -5.477   1.843  1.00  0.00	 11  
>>> ATOM    201 C217 POPE    2     -12.156  -6.088   0.154  1.00  0.00	 11  
>>> ATOM    202 H17R POPE    2     -11.427  -6.895  -0.073  1.00  0.00	 11  
>>> ATOM    203 H17S POPE    2     -13.164  -6.398  -0.194  1.00  0.00	 11  
>>> ATOM    204 C218 POPE    2     -11.667  -4.984  -0.769  1.00  0.00	 11  
>>> ATOM    205 H18R POPE    2     -10.615  -4.687  -0.572  1.00  0.00	 11  
>>> ATOM    206 H18S POPE    2     -11.858  -5.307  -1.815  1.00  0.00	 11  
>>> ATOM    207 H18T POPE    2     -12.274  -4.060  -0.660  1.00  0.00	 11  
>>> ATOM    208  C33 POPE    2     -18.924 -14.271  14.060  1.00  0.00	 11  
>>> ATOM    209  H3X POPE    2     -18.718 -15.246  13.570  1.00  0.00	 11  
>>> ATOM    210  H3Y POPE    2     -20.020 -14.362  14.219  1.00  0.00	 11  
>>> ATOM    211  C34 POPE    2     -18.653 -13.077  13.218  1.00  0.00	 11  
>>> ATOM    212  H4X POPE    2     -18.670 -12.145  13.824  1.00  0.00	 11  
>>> ATOM    213  H4Y POPE    2     -17.636 -13.194  12.787  1.00  0.00	 11  
>>> ATOM    214  C35 POPE    2     -19.632 -12.861  12.028  1.00  0.00	 11  
>>> ATOM    215  H5X POPE    2     -19.847 -13.760  11.411  1.00  0.00	 11  
>>> ATOM    216  H5Y POPE    2     -20.630 -12.537  12.392  1.00  0.00	 11  
>>> ATOM    217  C36 POPE    2     -19.197 -11.799  11.007  1.00  0.00	 11  
>>> ATOM    218  H6X POPE    2     -18.855 -10.943  11.626  1.00  0.00	 11  
>>> ATOM    219  H6Y POPE    2     -18.352 -12.353  10.546  1.00  0.00	 11  
>>> ATOM    220  C37 POPE    2     -20.284 -11.350   9.991  1.00  0.00	 11  
>>> ATOM    221  H7X POPE    2     -20.676 -12.197   9.387  1.00  0.00	 11  
>>> ATOM    222  H7Y POPE    2     -21.090 -10.803  10.525  1.00  0.00	 11  
>>> ATOM    223  C38 POPE    2     -19.519 -10.496   8.945  1.00  0.00	 11  
>>> ATOM    224  H8X POPE    2     -19.143  -9.581   9.452  1.00  0.00	 11  
>>> ATOM    225  H8Y POPE    2     -18.575 -11.029   8.701  1.00  0.00	 11  
>>> ATOM    226  C39 POPE    2     -20.343  -9.998   7.741  1.00  0.00	 11  
>>> ATOM    227  H9X POPE    2     -20.563 -10.858   7.073  1.00  0.00	 11  
>>> ATOM    228  H9Y POPE    2     -21.382  -9.715   8.012  1.00  0.00	 11  
>>> ATOM    229 C310 POPE    2     -19.744  -8.789   7.060  1.00  0.00	 11  
>>> ATOM    230 H10X POPE    2     -19.851  -7.979   7.814  1.00  0.00	 11  
>>> ATOM    231 H10Y POPE    2     -18.663  -9.003   6.920  1.00  0.00	 11  
>>> ATOM    232 C311 POPE    2     -20.500  -8.346   5.840  1.00  0.00	 11  
>>> ATOM    233 H11X POPE    2     -20.709  -9.171   5.126  1.00  0.00	 11  
>>> ATOM    234 H11Y POPE    2     -21.475  -7.934   6.180  1.00  0.00	 11  
>>> ATOM    235 C312 POPE    2     -19.817  -7.197   5.026  1.00  0.00	 11  
>>> ATOM    236 H12X POPE    2     -20.466  -6.802   4.215  1.00  0.00	 11  
>>> ATOM    237 H12Y POPE    2     -19.488  -6.300   5.593  1.00  0.00	 11  
>>> ATOM    238 C313 POPE    2     -18.536  -7.654   4.332  1.00  0.00	 11  
>>> ATOM    239 H13X POPE    2     -17.691  -7.842   5.027  1.00  0.00	 11  
>>> ATOM    240 H13Y POPE    2     -18.741  -8.676   3.948  1.00  0.00	 11  
>>> ATOM    241 C314 POPE    2     -18.015  -6.856   3.191  1.00  0.00	 11  
>>> ATOM    242 H14X POPE    2     -18.660  -6.829   2.288  1.00  0.00	 11  
>>> ATOM    243 H14Y POPE    2     -17.997  -5.799   3.532  1.00  0.00	 11  
>>> ATOM    244 C315 POPE    2     -16.608  -7.353   2.722  1.00  0.00	 11  
>>> ATOM    245 H15X POPE    2     -15.904  -7.207   3.569  1.00  0.00	 11  
>>> ATOM    246 H15Y POPE    2     -16.827  -8.417   2.490  1.00  0.00	 11  
>>> ATOM    247 C316 POPE    2     -16.157  -6.467   1.545  1.00  0.00	 11  
>>> ATOM    248 H16X POPE    2     -15.282  -6.911   1.025  1.00  0.00	 11  
>>> ATOM    249 H16Y POPE    2     -17.122  -6.400   0.998  1.00  0.00	 11  
>>> ATOM    250 H16Z POPE    2     -15.893  -5.459   1.932  1.00  0.00	 11  
>>  
>> -- 
>> ========================================
>>
>> Justin A. Lemkul
>> Ph.D. Candidate
>> ICTAS Doctoral Scholar
>> MILES-IGERT Trainee
>> Department of Biochemistry
>> Virginia Tech
>> Blacksburg, VA
>> jalemkul[at]vt.edu | (540) 231-9080
>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>> ========================================
>> -- 
>> gmx-users mailing list    gmx-users at gromacs.org
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>> www interface or send it to gmx-users-request at gromacs.org.
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> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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