[gmx-users] GROMACS/ORCA QMMM

Justin A. Lemkul jalemkul at vt.edu
Thu Nov 17 19:48:11 CET 2011



Jose Tusell wrote:
> Hi Justin,
> 
> Thanks for the input on why this is happening.   It sounds a little
> suspicious that the energy doesn't change after a few steps of energy
> minimization.  Do you know of any way that I can find out what is
> going on?
> 

The screen output should indicate the atom with maximal force.  Sometimes the EM 
algorithms get stuck when the geometry cannot change without making detrimental 
moves.  You either need a larger step size, a different algorithm, or a better 
starting structure, if that is the case.  I have seen this many times before, 
nothing suspicious about it.

-Justin

> Thanks,
> 
> Jose Tusell
> 
> On Thu, Nov 17, 2011 at 10:58 AM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
>>
>> Jose Tusell wrote:
>>> Hi Cristoph,
>>>
>>> Thanks for the reply.  I found that my problem was not gromacs.  The
>>> input that ORCA was receiving from GROMACS did not have the correct
>>> number of hydrogens.  I've solved this problem now and ORCA is running
>>> fine.  I however ran into another problem with my energy minimization.
>>>  The output from my gromacs log file is the following:
>>>
>>>           Step           Time         Lambda
>>>              0        0.00000        0.00000
>>>
>>>   Energies (kJ/mol)
>>>           Bond          Angle    Proper Dih.  Improper Dih.          LJ-14
>>>    1.21899e+04    1.92496e+03    5.62567e+03    1.49141e+01    3.68001e+03
>>>     Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.    Quantum En.
>>>    2.41200e+04    1.47494e+05   -5.06544e+05   -7.56009e+04   -3.96508e+06
>>>      Potential Pressure (bar)
>>>   -4.35218e+06   -2.10629e+04
>>>
>>>           Step           Time         Lambda
>>>              1        1.00000        0.00000
>>>
>>>   Energies (kJ/mol)
>>>           Bond          Angle    Proper Dih.  Improper Dih.          LJ-14
>>>    1.20006e+04    1.92297e+03    5.62600e+03    1.47801e+01    3.67746e+03
>>>     Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.    Quantum En.
>>>    2.41174e+04    1.46421e+05   -5.06609e+05   -7.56156e+04   -4.00402e+06
>>>      Potential Pressure (bar)
>>>   -4.39246e+06   -2.10739e+04
>>>
>>>           Step           Time         Lambda
>>>              2        2.00000        0.00000
>>>
>>>   Energies (kJ/mol)
>>>           Bond          Angle    Proper Dih.  Improper Dih.          LJ-14
>>>    1.17961e+04    1.92126e+03    5.62633e+03    1.46477e+01    3.67461e+03
>>>     Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.    Quantum En.
>>>    2.41145e+04    1.45528e+05   -5.06679e+05   -7.56315e+04   -4.18671e+06
>>>      Potential Pressure (bar)
>>>   -4.57635e+06   -2.10854e+04
>>>
>>>           Step           Time         Lambda
>>>              3        3.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>              4        4.00000        0.00000
>>>
>>>   Energies (kJ/mol)
>>>           Bond          Angle    Proper Dih.  Improper Dih.          LJ-14
>>>    1.16705e+04    1.92041e+03    5.62652e+03    1.45728e+01    3.67282e+03
>>>     Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.    Quantum En.
>>>    2.41128e+04    1.45113e+05   -5.06721e+05   -7.56410e+04   -4.24486e+06
>>>      Potential Pressure (bar)
>>>   -4.63509e+06   -2.10913e+04
>>>
>>>           Step           Time         Lambda
>>>              5        5.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>              6        6.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>              7        7.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>              8        8.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>              9        9.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             10       10.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             11       11.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             12       12.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             13       13.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             14       14.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             15       15.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             16       16.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             17       17.00000        0.00000
>>>
>>>           Step           Time         Lambda
>>>             18       18.00000        0.00000
>>>
>>>
>>> Stepsize too small, or no change in energy.
>>> Converged to machine precision,
>>> but not to the requested precision Fmax < 1000
>>>
>>> Double precision normally gives you higher accuracy.
>>> You might need to increase your constraint accuracy, or turn
>>> off constraints alltogether (set constraints = none in mdp file)
>>>
>>> Why doesn't GROMACS output the energies for certain steps?  Step 3 and
>>> steps 5-18 do show any output in the log file.  Any ideas why this is
>>> happening?
>>>
>> This happens for the reasons printed by mdrun - those steps caused no change
>> in energy.
>>
>> -Justin
>>
>> --
>> ========================================
>>
>> Justin A. Lemkul
>> Ph.D. Candidate
>> ICTAS Doctoral Scholar
>> MILES-IGERT Trainee
>> Department of Biochemistry
>> Virginia Tech
>> Blacksburg, VA
>> jalemkul[at]vt.edu | (540) 231-9080
>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
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> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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