[gmx-users] GROMACS/ORCA QMMM

Jose Tusell jrta1981 at gmail.com
Tue Nov 22 04:02:03 CET 2011


Does anyone know what is going on with the coordinates of the QM
region?  Why are they not converted to the correct units?  Could
anyone point me to the error?

Thanks

Jose Tusell

On Sun, Nov 20, 2011 at 10:06 PM, Jose Tusell <jrta1981 at gmail.com> wrote:
> Yes.  But where does the code go wrong?  I'm reading the qm_orca.c
> file but I can't seem to know exactly were the factor of 10 is not
> accounted for.  I see the factor of ten when going from gromacs to
> orca in (lines 219 to 221 of qm_orca.c file):
>
>     qm->xQM[i][XX]/0.1,
>     qm->xQM[i][YY]/0.1,
>     qm->xQM[i][ZZ]/0.1);
>
> I just don't know where to look for the factor of 10 in the code.
> Could anyone point me to this?
>
> Thanks,
>
> Jose Tusell
>
> On Sun, Nov 20, 2011 at 10:00 PM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
>>
>>
>> Jose Tusell wrote:
>>>
>>> When I run gromacs with bOpt yes I run into the a problem with the
>>> coordinates of my qm system: the initial coordinates are the
>>> following:
>>>
>>> NAME = ybu_em_qmmm.inp
>>> |  1> #input-file generated by gromacs
>>> |  2> !QMMMOpt TightSCF
>>> |  3> !PAL8 Quick-DFT VerySlowConv TightSCF KDIIS VDW EnGrad NoUseSym
>>> |  4> %scf
>>> |  5>   Maxiter 5000
>>> |  6> end
>>> |  7> %pal nprocs 8
>>> |  8> end
>>> |  9>
>>> | 10>
>>> | 11>
>>> | 12> %LJCOEFFICIENTS "ybu_em_qmmm.LJ"
>>> | 13> *xyz -2 1
>>> | 14>   7 46.4499998  40.2500010  48.1099987
>>> | 15>   6 45.2400017  40.2899981  47.5600004
>>> | 16>   7 44.7599983  40.6899977  46.3600016
>>> | 17>   6 43.4700012  40.5999994  46.0599995
>>> | 18>   7 42.6300001  40.1100016  46.9500017
>>> | 19>   6 43.0100012  39.6799994  48.1699991
>>> | 20>   7 42.1299982  39.1499996  49.0199995
>>> | 21>   1 42.4300003  38.8100004  49.9200010
>>> | 22>   1 41.1600018  39.0899992  48.7699986
>>> | 23>   6 44.3400002  39.7700000  48.4999990
>>> | 24>   7 45.0799990  39.4600010  49.5800018
>>> | 25>   6 46.3500023  39.7600007  49.3499994
>>> | 26>   6 47.7699995  40.5399990  47.4900007
>>> | 27>   8 48.8000011  41.0900021  48.3400011
>>> | 28>   6 49.8199987  41.7100000  47.4800014
>>> | 29>   6 47.7400017  41.5500021  46.3600016
>>> | 30>   8 46.9999981  41.4499998  45.1499987
>>> | 31>   1 47.2300005  40.5999994  44.6799994
>>> | 32>   6 49.1900015  41.9199991  46.1000013
>>> | 33>   8 49.7800016  40.8699989  45.3399992
>>> | 34>   1 50.7399988  41.0900021  45.1599979
>>> | 35>   6 50.0799990  43.2399988  47.5799990
>>> | 36>   8 51.4400005  43.5300016  47.9400015
>>> | 37>  15 51.8400002  43.3099985  49.4799995
>>> | 38>   8 52.1000004  44.8099995  49.9800014
>>> | 39>   8 50.5700016  42.5000000  50.0600004
>>> | 40>   8 53.1099987  42.3299980  49.3599987
>>> | 41>  15 54.4700003  43.1799984  49.4500017
>>> | 42>   8 55.5600023  42.1400023  48.8899994
>>> | 43>   8 54.1200018  44.4799995  48.5599995
>>> | 44>   8 54.5300007  43.5900021  50.9999990
>>> | 45>  15 55.8799982  43.3500004  51.8499994
>>> | 46>   8 57.0900011  43.9900017  50.9899998
>>> | 47>   8 55.8300018  43.9099979  53.2200003
>>> | 48>   8 56.1399984  41.7600012  51.8499994
>>> | 49>   1 43.1099987  40.9200001  45.1000023
>>> | 50>   1 47.1600008  39.6399999  50.0400019
>>> | 51>   1 48.1400013  39.6199989  47.0900011
>>> | 52>   1 47.3199987  42.4200010  46.8200016
>>> | 53>   1 49.3300009  42.8999996  45.7000017
>>> | 54>   1 50.7200003  41.1499977  47.4200010
>>> | 55>   1 49.8600006  43.6999989  46.6300011
>>> | 56>   1 49.4299984  43.6600018  48.3199978
>>> | 57>  12 51.3999987  43.3799982  51.9299984
>>> | 58> *
>>> | 59> %pointcharges "ybu_em_qmmm.pc"
>>> | 60>
>>> | 61>
>>> | 62>                          ****END OF INPUT****
>>>
>>> This first calculation doesn't run into problems however after this
>>> the new coordinates are wrong by a factor of 10.
>>>
>>> This is the next input file generated by gromacs:
>>>
>>> NAME = ybu_em_qmmm.inp
>>> |  1> #input-file generated by gromacs
>>> |  2> !QMMMOpt TightSCF
>>> |  3> !PAL8 Quick-DFT VerySlowConv TightSCF KDIIS VDW EnGrad NoUseSym
>>> |  4> %scf
>>> |  5>   Maxiter 5000
>>> |  6> end
>>> |  7> %pal nprocs 8
>>> |  8> end
>>> |  9>
>>> | 10>
>>> | 11>
>>> | 12> %LJCOEFFICIENTS "ybu_em_qmmm.LJ"
>>> | 13> *xyz -2 1
>>> | 14>   7  4.6643215   4.0067959   4.8652828
>>> | 15>   6  4.4694147   4.0687612   4.6910113
>>> | 16>   7  4.4788954   4.0736303   4.5814043
>>> | 17>   6  4.2882785   4.0960234   4.5192298
>>> | 18>   7  4.1568694   3.9851558   4.6636197
>>> | 19>   6  4.2702511   3.9942223   4.8413402
>>> | 20>   7  4.1388491   3.8647231   4.9325246
>>> | 21>   1  4.2422304   3.8729668   5.0225985
>>> | 22>   1  4.0874949   3.9029175   4.8758829
>>> | 23>   6  4.4479644   3.9264068   4.8907641
>>> | 24>   7  4.4724247   3.8935867   5.0617158
>>> | 25>   6  4.6581322   3.9623603   4.9860328
>>> | 26>   6  4.7224784   3.9516553   4.7729388
>>> | 27>   8  4.9013394   4.1037330   4.9433815
>>> | 28>   6  5.0506485   4.1505158   4.7845429
>>> | 29>   6  4.7479546   4.2596489   4.5867884
>>> | 30>   8  4.6396983   4.1753584   4.3912268
>>> | 31>   1  4.7245148   4.0333679   4.4395000
>>> | 32>   6  4.9467531   4.1628179   4.5303190
>>> | 33>   8  5.0127959   4.0332937   4.4357231
>>> | 34>   1  5.0972128   4.1086709   4.5025694
>>> | 35>   6  4.9707726   4.4155064   4.7358957
>>> | 36>   8  5.1747119   4.3822494   4.7240046
>>> | 37>  15  5.1759595   4.3526104   4.9696961
>>> | 38>   8  5.2000558   4.5951414   5.0385225
>>> | 39>   8  4.9872991   4.2056358   5.0856835
>>> | 40>   8  5.3196424   4.1316289   4.9306369
>>> | 41>  15  5.4781163   4.3365851   4.9144840
>>> | 42>   8  5.6436682   4.1501650   4.8309124
>>> | 43>   8  5.4556131   4.5460758   4.7758737
>>> | 44>   8  5.4388809   4.3959689   5.1872373
>>> | 45>  15  5.6339312   4.3419433   5.2184927
>>> | 46>   8  5.8053279   4.4479111   5.0789297
>>> | 47>   8  5.5981785   4.4297397   5.4245871
>>> | 48>   8  5.6559128   4.0851763   5.2241915
>>> | 49>   1  4.2944524   4.1013804   4.4863331
>>> | 50>   1  4.7380015   3.9515409   5.0357658
>>> | 51>   1  4.8261809   3.9258796   4.7002071
>>> | 52>   1  4.7115117   4.2592505   4.6899751
>>> | 53>   1  4.9388400   4.3221569   4.5484522
>>> | 54>   1  5.1027077   4.0887082   4.7342393
>>> | 55>   1  4.9870053   4.3987840   4.6446544
>>> | 56>   1  4.9254298   4.3886098   4.8489299
>>> | 57>  12  5.1316565   4.3423077   5.2266484
>>> | 58> *
>>> | 59> %pointcharges "ybu_em_qmmm.pc"
>>> | 60>
>>> | 61>
>>> | 62>                          ****END OF INPUT****
>>>
>>> Any ideas why this is happening?
>>>
>>
>> A factor of 10 would seem to me to be an Angstrom -> nm conversion.
>>
>> -Justin
>>
>>> Thanks,
>>>
>>> Jose Tusell
>>>
>>> On Sun, Nov 20, 2011 at 5:03 PM, Jose Tusell <jrta1981 at gmail.com> wrote:
>>>>
>>>> That solved it.  In the ORCA manual for version 2.8 it says to use
>>>> bOpt = true instead of bOpt = yes.  And there is no mention of the mdp
>>>> option in the gromacs manual or gromacs online mdp options.
>>>>
>>>> Ramon
>>>>
>>>> On Sun, Nov 20, 2011 at 4:57 PM, Jose Tusell <jrta1981 at gmail.com> wrote:
>>>>>
>>>>> I've tried the bOpt = true.  Is the flag bOpt = yes?  I'll try that
>>>>> and I'll let you know.
>>>>>
>>>>> Ramon
>>>>>
>>>>> On Sun, Nov 20, 2011 at 1:19 PM, Christoph Riplinger
>>>>> <cri at thch.uni-bonn.de> wrote:
>>>>>>
>>>>>> Dear Justin,
>>>>>>
>>>>>> The LJ and LJ.Excl files should be generated when you set bOpt = yes.
>>>>>> What
>>>>>> does the .inp file say. Is there !QMMMOpt or only !EnGrad?
>>>>>> Christoph
>>>>>>
>>>>>> On 11/18/2011 06:20 PM, Jose Tusell wrote:
>>>>>>>
>>>>>>> Hi Justin,
>>>>>>>
>>>>>>> I tried using a larger step size but my system blew up...  My guess is
>>>>>>> that I have a bad starting structure, I'll work on the initial
>>>>>>> structure for the QM region (energy minimize).
>>>>>>>
>>>>>>> When I'm running ORCA I want to have a LJ file to correct for the
>>>>>>> interaction of my QM system with the protein.  However this file is
>>>>>>> not created when I run QMMM, I've used the bOpt = true in the mdp
>>>>>>> file.  Can anyone please offer some help on how to create this LJ
>>>>>>> files?
>>>>>>>
>>>>>>> Thanks,
>>>>>>>
>>>>>>> Jose Tusell
>>>>>>>
>>>>>>> On Thu, Nov 17, 2011 at 2:07 PM, swati patel<swatipatel088 at gmail.com>
>>>>>>>  wrote:
>>>>>>>>
>>>>>>>> hello,
>>>>>>>> I am trying to simulate streptavidin tetramer-biotin complex.I ve
>>>>>>>> calculated
>>>>>>>> Ligand topology from a sofware PRODRG using gromos87 force
>>>>>>>> fields.After
>>>>>>>> solvating it,I am getting an error using grompp command
>>>>>>>>
>>>>>>>> Fatal error:
>>>>>>>> Atomtype HW not found
>>>>>>>>
>>>>>>>> can anyone provide me some help?
>>>>>>>>
>>>>>>>> Thanx with anticipation.
>>>>>>>>
>>>>>>>>
>>>>>>>> On Fri, Nov 18, 2011 at 2:29 AM, Jose Tusell<jrta1981 at gmail.com>
>>>>>>>>  wrote:
>>>>>>>>>
>>>>>>>>> I'll try changing the step size first and if that fails I'll try
>>>>>>>>> another algorithm.  Thanks for the input.
>>>>>>>>>
>>>>>>>>> Jose Tusell
>>>>>>>>>
>>>>>>>>> On Thu, Nov 17, 2011 at 11:48 AM, Justin A. Lemkul<jalemkul at vt.edu>
>>>>>>>>> wrote:
>>>>>>>>>>
>>>>>>>>>> Jose Tusell wrote:
>>>>>>>>>>>
>>>>>>>>>>> Hi Justin,
>>>>>>>>>>>
>>>>>>>>>>> Thanks for the input on why this is happening.   It sounds a
>>>>>>>>>>> little
>>>>>>>>>>> suspicious that the energy doesn't change after a few steps of
>>>>>>>>>>> energy
>>>>>>>>>>> minimization.  Do you know of any way that I can find out what is
>>>>>>>>>>> going on?
>>>>>>>>>>>
>>>>>>>>>> The screen output should indicate the atom with maximal force.
>>>>>>>>>>  Sometimes
>>>>>>>>>> the EM algorithms get stuck when the geometry cannot change without
>>>>>>>>>> making
>>>>>>>>>> detrimental moves.  You either need a larger step size, a different
>>>>>>>>>> algorithm, or a better starting structure, if that is the case.  I
>>>>>>>>>> have
>>>>>>>>>> seen
>>>>>>>>>> this many times before, nothing suspicious about it.
>>>>>>>>>>
>>>>>>>>>> -Justin
>>>>>>>>>>
>>>>>>>>>>> Thanks,
>>>>>>>>>>>
>>>>>>>>>>> Jose Tusell
>>>>>>>>>>>
>>>>>>>>>>> On Thu, Nov 17, 2011 at 10:58 AM, Justin A.
>>>>>>>>>>> Lemkul<jalemkul at vt.edu>
>>>>>>>>>>> wrote:
>>>>>>>>>>>>
>>>>>>>>>>>> Jose Tusell wrote:
>>>>>>>>>>>>>
>>>>>>>>>>>>> Hi Cristoph,
>>>>>>>>>>>>>
>>>>>>>>>>>>> Thanks for the reply.  I found that my problem was not gromacs.
>>>>>>>>>>>>>  The
>>>>>>>>>>>>> input that ORCA was receiving from GROMACS did not have the
>>>>>>>>>>>>> correct
>>>>>>>>>>>>> number of hydrogens.  I've solved this problem now and ORCA is
>>>>>>>>>>>>> running
>>>>>>>>>>>>> fine.  I however ran into another problem with my energy
>>>>>>>>>>>>> minimization.
>>>>>>>>>>>>>  The output from my gromacs log file is the following:
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            0        0.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>  Energies (kJ/mol)
>>>>>>>>>>>>>         Bond          Angle    Proper Dih.  Improper Dih.
>>>>>>>>>>>>>  LJ-14
>>>>>>>>>>>>>  1.21899e+04    1.92496e+03    5.62567e+03    1.49141e+01
>>>>>>>>>>>>>  3.68001e+03
>>>>>>>>>>>>>   Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.
>>>>>>>>>>>>>  Quantum
>>>>>>>>>>>>> En.
>>>>>>>>>>>>>  2.41200e+04    1.47494e+05   -5.06544e+05   -7.56009e+04
>>>>>>>>>>>>> -3.96508e+06
>>>>>>>>>>>>>    Potential Pressure (bar)
>>>>>>>>>>>>>  -4.35218e+06   -2.10629e+04
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            1        1.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>  Energies (kJ/mol)
>>>>>>>>>>>>>         Bond          Angle    Proper Dih.  Improper Dih.
>>>>>>>>>>>>>  LJ-14
>>>>>>>>>>>>>  1.20006e+04    1.92297e+03    5.62600e+03    1.47801e+01
>>>>>>>>>>>>>  3.67746e+03
>>>>>>>>>>>>>   Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.
>>>>>>>>>>>>>  Quantum
>>>>>>>>>>>>> En.
>>>>>>>>>>>>>  2.41174e+04    1.46421e+05   -5.06609e+05   -7.56156e+04
>>>>>>>>>>>>> -4.00402e+06
>>>>>>>>>>>>>    Potential Pressure (bar)
>>>>>>>>>>>>>  -4.39246e+06   -2.10739e+04
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            2        2.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>  Energies (kJ/mol)
>>>>>>>>>>>>>         Bond          Angle    Proper Dih.  Improper Dih.
>>>>>>>>>>>>>  LJ-14
>>>>>>>>>>>>>  1.17961e+04    1.92126e+03    5.62633e+03    1.46477e+01
>>>>>>>>>>>>>  3.67461e+03
>>>>>>>>>>>>>   Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.
>>>>>>>>>>>>>  Quantum
>>>>>>>>>>>>> En.
>>>>>>>>>>>>>  2.41145e+04    1.45528e+05   -5.06679e+05   -7.56315e+04
>>>>>>>>>>>>> -4.18671e+06
>>>>>>>>>>>>>    Potential Pressure (bar)
>>>>>>>>>>>>>  -4.57635e+06   -2.10854e+04
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            3        3.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            4        4.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>  Energies (kJ/mol)
>>>>>>>>>>>>>         Bond          Angle    Proper Dih.  Improper Dih.
>>>>>>>>>>>>>  LJ-14
>>>>>>>>>>>>>  1.16705e+04    1.92041e+03    5.62652e+03    1.45728e+01
>>>>>>>>>>>>>  3.67282e+03
>>>>>>>>>>>>>   Coulomb-14        LJ (SR)   Coulomb (SR)   Coul. recip.
>>>>>>>>>>>>>  Quantum
>>>>>>>>>>>>> En.
>>>>>>>>>>>>>  2.41128e+04    1.45113e+05   -5.06721e+05   -7.56410e+04
>>>>>>>>>>>>> -4.24486e+06
>>>>>>>>>>>>>    Potential Pressure (bar)
>>>>>>>>>>>>>  -4.63509e+06   -2.10913e+04
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            5        5.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            6        6.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            7        7.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            8        8.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>            9        9.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           10       10.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           11       11.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           12       12.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           13       13.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           14       14.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           15       15.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           16       16.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           17       17.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>         Step           Time         Lambda
>>>>>>>>>>>>>           18       18.00000        0.00000
>>>>>>>>>>>>>
>>>>>>>>>>>>>
>>>>>>>>>>>>> Stepsize too small, or no change in energy.
>>>>>>>>>>>>> Converged to machine precision,
>>>>>>>>>>>>> but not to the requested precision Fmax<  1000
>>>>>>>>>>>>>
>>>>>>>>>>>>> Double precision normally gives you higher accuracy.
>>>>>>>>>>>>> You might need to increase your constraint accuracy, or turn
>>>>>>>>>>>>> off constraints alltogether (set constraints = none in mdp file)
>>>>>>>>>>>>>
>>>>>>>>>>>>> Why doesn't GROMACS output the energies for certain steps?  Step
>>>>>>>>>>>>> 3
>>>>>>>>>>>>> and
>>>>>>>>>>>>> steps 5-18 do show any output in the log file.  Any ideas why
>>>>>>>>>>>>> this
>>>>>>>>>>>>> is
>>>>>>>>>>>>> happening?
>>>>>>>>>>>>>
>>>>>>>>>>>> This happens for the reasons printed by mdrun - those steps
>>>>>>>>>>>> caused no
>>>>>>>>>>>> change
>>>>>>>>>>>> in energy.
>>>>>>>>>>>>
>>>>>>>>>>>> -Justin
>>>>>>>>>>>>
>>>>>>>>>>>> --
>>>>>>>>>>>> ========================================
>>>>>>>>>>>>
>>>>>>>>>>>> Justin A. Lemkul
>>>>>>>>>>>> Ph.D. Candidate
>>>>>>>>>>>> ICTAS Doctoral Scholar
>>>>>>>>>>>> MILES-IGERT Trainee
>>>>>>>>>>>> Department of Biochemistry
>>>>>>>>>>>> Virginia Tech
>>>>>>>>>>>> Blacksburg, VA
>>>>>>>>>>>> jalemkul[at]vt.edu | (540) 231-9080
>>>>>>>>>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>>>>>>>>>>
>>>>>>>>>>>> ========================================
>>>>>>>>>>>> --
>>>>>>>>>>>> gmx-users mailing list    gmx-users at gromacs.org
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>>>>>>>>>>>>
>>>>>>>>>> --
>>>>>>>>>> ========================================
>>>>>>>>>>
>>>>>>>>>> Justin A. Lemkul
>>>>>>>>>> Ph.D. Candidate
>>>>>>>>>> ICTAS Doctoral Scholar
>>>>>>>>>> MILES-IGERT Trainee
>>>>>>>>>> Department of Biochemistry
>>>>>>>>>> Virginia Tech
>>>>>>>>>> Blacksburg, VA
>>>>>>>>>> jalemkul[at]vt.edu | (540) 231-9080
>>>>>>>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>>>>>>>>
>>>>>>>>>> ========================================
>>>>>>>>>> --
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>>>>>>>>> --
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>>>>>> --
>>>>>> gmx-users mailing list    gmx-users at gromacs.org
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>>
>> --
>> ========================================
>>
>> Justin A. Lemkul
>> Ph.D. Candidate
>> ICTAS Doctoral Scholar
>> MILES-IGERT Trainee
>> Department of Biochemistry
>> Virginia Tech
>> Blacksburg, VA
>> jalemkul[at]vt.edu | (540) 231-9080
>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>> ========================================
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>



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