[gmx-users] A question about simulating a box of ethane in GROMACS

Phil (Yang) Song ysong at bu.edu
Tue Sep 6 21:23:30 CEST 2011


Hi,

Thank everyone for your suggestions.

I have tried to put less molecule in a small box and find this works!
Eventually I have solve the problem by using a larger initial box size for
the random generated position and orientation. It seemed that the overall
interaction of OPLS-AA between ethanes is attractive rather than repulsive,
which leads to atomic clashes...

The original box size was calculated from experimental density but
apparently this does not work for the OPLS-AA. I had used larger initial box
size previously (20% larger than experimental calculated box size) but it
did not work out and then I gave up on this direction. Now, I have tried to
enlarge the box size by 40% and everything works!

Finally I should offer my gratitude to all of you. Thank you!

Best,
Phil

On Tue, Sep 6, 2011 at 10:59 AM, Mark Abraham <Mark.Abraham at anu.edu.au>wrote:

>  On 7/09/2011 12:40 AM, Phil (Yang) Song wrote:
>
>   Hi, all
>
> I am learning GROMACS 4.5.1 recently and encountered a problem that puzzled
> me for weeks. I am wondering if someone can help me with this.
>
> Here is the problem: I was trying to perform a MD simulation for 216 ethane
> molecules in a box. I have first generated a box of ethane with random
> position and orientation.
>
>
> That's almost certainly inappropriate. Atomic clashes are inevitable, and
> EM will not fix severe problems.
>
> Get a single molecule in a small box, and use genconf to replicate it. Then
> use EM and equilibrate thoroughly to remove the residual ordering. Judicious
> use of NPT will fix your density to whatever you want.
>
> Mark
>
>
>    Then, I wanted to run a minimization for the box of ethane to get rid
> of close contact of the randomly ethane molecules. However, I have found
> that some of the ethane molecules laid on top of each other after
> minimization and infinity forces were resulted.
>
> Since my mdp file was successfully used to generate other type of molecules
> such as acetylene and ethylene and they should be correct. Also, I have
> tried to use conjugate gradient instead of steepest decent for the
> minimization, but this effort came out to be in vain.
>
> Later, I thought the topology file should be the source of error. I have
> carefully checked the topology file for couple of times but did not find any
> obvious error. I have also tried to change the charges on each atom in the
> ethane to 0 and then run the minimization. Again, the ethane was attracted
> into each other. Hence, I think the vdw could be the problem. However, the
> parameters of the vdw interaction are all from the opls-aa files that come
> with gromacs and it should be fine.
>
> Eventually, I am puzzled...
>
> I am iterating the content of the content of mdp input file and itp file in
> the email and also attaching the input and output of my result as a tarball.
> Hope someone can help me to solve this problem.
>
> Thank you in advance!
>
> Best,
> Phil Song
>
>
> ================================================================================
> MDP file
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; preprocessing options
>
> ; title of the simulation
> title        =
>
> ; include path
> include      =
>
> ; cpp flag
> define       =
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; energy minimization
> integrator    = steep
>
> ; Start time and timestep in ps
> tinit         = 0
> dt            = 0.001
> nsteps        = 500
>
> ; run continuation or reperforming
> init_step     = 0
>
> emtol         = 1000.0
> emstep        = 0.01
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; periodic boundary conditions
> pbc           = xyz
>
> periodic_molecules = no
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; electrostatics and vdw options
> ; electrostatics method
> coulombtype    = PME
> rcoulomb       = 1.3
>
> ; fourier setup for PME
> fourierspacing = 0.12
> fourier_nx     = 0
> fourier_ny     = 0
> fourier_nz     = 0
> pme_order      = 4
> ewald_rtol     = 1e-5
> optimize_fft   = yes
>
> ewald_geometry = 3d
>
> ; vdw cutoff
> rvdw-switch    = 0
> rvdw           = 1.31
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; neighbor list options
> ; neighbor list frequency
> nstlist       = 10
>
> ; neighbor search algorithm
> ns_type       = grid
>
> ; neighbor list cut-off
> rlist         = 1.30
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; thermostat and barostat
> tcoupl            = v-rescale
> tc_grps         = System
> ref_t             = 50.5
> tau_t             = 0.5
>
> pcoupl            = berendsen
> pcoupltype        = isotropic
> nstpcouple        = 10
> ref_p             = 1.01325
> tau_p             = 1.0
>
> ; Using compressibility of ethanol at 273.1 (approximately)
> ; from J. Phys. Chem., 1963, 67 (10), pp 2160.2164
>
> compressibility = 1.0e-5
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; velocty generation
> gen_vel       = yes
> gen_temp      = 50.5
> ; gen_seed      = 173529
>
> ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
> ; output control options
> ; output frequency for coords, velocities and forces
> nstxout       = 5000
> nstvout       = 5000
> nstfout       = 5000
>
> ; output frequency for energy
> nstenergy     = 5000
> ; energygrps    =
>
> ; output frequency for log
> nstlog        = 5000
>
> ; output options for trajectory
> nstxtcout     = 5000
> xtc_precision = 100000
> ; xtc_grps      =
>
>
>
> ================================================================================
> ITP file
>
> ; Ethane:
> ; Assign of the atom index
> ;
> ;      H2   H6
> ;      |    |
> ; H3 - C1 - C5 - H7
> ;      |    |
> ;      H4   H8
> ;
>
> [ moleculetype ]
> ;     name        nrexcl
>     Ethane             3
>
> [ atoms ]
> ;   nr      type   resnr   residu    atom    cgnr      charge          mass
>      1  opls_135       1      ETH     CE1      1        -0.18
>      2  opls_140       1      ETH     HE2      1         0.06
>      3  opls_140       1      ETH     HE3      1         0.06
>      4  opls_140       1      ETH     HE4      1         0.06
>      5  opls_135       1      ETH     CE5      2        -0.18
>      6  opls_140       1      ETH     HE6      2         0.06
>      7  opls_140       1      ETH     HE7      2         0.06
>      8  opls_140       1      ETH     HE8      2         0.06
>
> [ bonds ]
> ;  ai  aj   funct                 c0            c1
>     1   5       1
>     2   1       1
>     3   1       1
>     4   1       1
>     6   5       1
>     7   5       1
>     8   5       1
>
> [ pairs ]
> ;  ai  aj   funct
>     2   6
>     2   7
>     2   8
>     3   6
>     3   7
>     3   8
>     4   6
>     4   7
>     4   8
>
> [ angles ]
> ;  ai    aj    ak  funct          c0            c1
> ;  H3-C-C
>     2     1     5      1
>     3     1     5      1
>     4     1     5      1
> ;  H-C-H
>     2     1     3      1
>     2     1     4      1
>     3     1     4      1
> ;  C-C-H3
>     6     5     1      1
>     7     5     1      1
>     8     5     1      1
> ;  H-C-H
>     6     5     7      1
>     6     5     8      1
>     7     5     8      1
>
> [ dihedrals ]
> ;  ai    aj    ak    al  funct       c0        c1        c2
> c3        c4
>     2     1     5     6      3
>     3     1     5     6      3
>     4     1     5     6      3
>     2     1     5     7      3
>     3     1     5     7      3
>     4     1     5     7      3
>     2     1     5     8      3
>     3     1     5     8      3
>     4     1     5     8      3
>
>
>
> --
> Phil (Yang) Song
> Room 509, 590 Comm. Ave.
> Chem. Dept., Boston Univ.
> Boston, MA, 02215, USA
>
>
>
>
> --
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-- 
Phil (Yang) Song
Room 509, 590 Comm. Ave.
Chem. Dept., Boston Univ.
Boston, MA, 02215, USA
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