[gmx-users] g_dist error

aiswarya pawar aiswarya.pawar at gmail.com
Tue Sep 13 08:14:52 CEST 2011


Mark,

the command line goes like this-

g_dist -f md.xtc -s md.tpr -dist 0.5 -e 500

the index file has group1- a_1322 ( this is just a single atom from a
protein. its in sidechain)
                          group2- a_OW ( this is water atoms)

now as per the utility it should give me and output as-

t:1 1322 a 54119 OW 0.389

but am getting something different

On Tue, Sep 13, 2011 at 11:24 AM, Mark Abraham <Mark.Abraham at anu.edu.au>wrote:

>  On 13/09/2011 3:40 PM, aiswarya pawar wrote:
>
>
> Hi Mark,
>
> The -dist option says- print all the atoms in group 2 that are closer than
> a certain distance to the center of mass of group 1.
> That means it should give me the distance from OW to protein atom.
>
>
> If you choose correct groups that are correctly defined with respect to
> your trajectory and use a large enough distance cutoff.
>
>
>
> And when am already specifying only one atom from protein ie say 1322. why
> do i get this kind of output-
>
>
> We can't tell. We don't know what your command line is, what's in your
> simulation system, the contents of your index groups, or which groups you've
> selected for which role. Clearly something is not working properly, and our
> time constraints mean that we're going to assume you've done something
> wrong, in the absence of evidence to the contrary.
>
> Mark
>
>
>
> t: 275  20230 SOL 62618 OW  0.341434 (nm)
> t: 275  22019 SOL 67985 OW  0.171584 (nm)
> t: 276  10768 SOL 34232 OW  0.303328 (nm)
> t: 276  20230 SOL 62618 OW  0.325176 (nm)
> t: 276  22019 SOL 67985 OW  0.187259 (nm)
> t: 277  10768 SOL 34232 OW  0.306008 (nm)
> t: 277  20230 SOL 62618 OW  0.326195 (nm)
> t: 277  22019 SOL 67985 OW  0.181089 (nm)
> t: 278  10768 SOL 34232 OW  0.274507 (nm)
> t: 278  22019 SOL 67985 OW  0.292652 (nm)
> t: 279  10618 SOL 33782 OW  0.319922 (nm)
> t: 280  10618 SOL 33782 OW  0.330082 (nm)
> t: 280  22019 SOL 67985 OW  0.330203 (nm)
> t: 281  8273 SOL 26747 OW  0.278731 (nm)
> t: 281  10618 SOL 33782 OW  0.325434 (nm)
> t: 281  11535 SOL 36533 OW  0.200327 (nm)
> t: 281  17036 SOL 53036 OW  0.343946 (nm)
> t: 282  8273 SOL 26747 OW  0.256558 (nm)
> t: 282  11535 SOL 36533 OW  0.327147 (nm)
> t: 283  8273 SOL 26747 OW  0.165061 (nm)
> t: 283  10618 SOL 33782 OW  0.306578 (nm)
> t: 283  17191 SOL 53501 OW  0.333075 (nm)
> t: 284  8273 SOL 26747 OW  0.19427 (nm)
> t: 284  10618 SOL 33782 OW  0.321927 (nm)
> t: 284  17191 SOL 53501 OW  0.30832 (nm)
>
>
>
>
> On Tue, Sep 13, 2011 at 10:40 AM, Mark Abraham <Mark.Abraham at anu.edu.au>wrote:
>
>>  On 13/09/2011 2:27 PM, aiswarya.pawar at gmail.com wrote:
>>
>> Iam -dist option because I need the distance between two groups
>>
>>
>>  That is not what g_dist -dist does. Please read g_dist -h.
>>
>>
>>   excluding -dist gives me X,Y,Z output which I don't want.
>>
>>
>>  And other output which you do, but you have to use -o to get it. Read
>> g_dist -h.
>>
>>
>>   And am not specifying an -o.
>>
>>
>>  You need to specify -o to achieve your purpose. However, as I said quite
>> a while ago, there is no value in measuring the distance between a protein
>> phase and a water phase if they are in contact...
>>
>> Mark
>>
>>
>>  Sent from my BlackBerry® on Reliance Mobile, India's No. 1 Network. Go for it!
>>
>> -----Original Message-----
>> From: "Justin A. Lemkul" <jalemkul at vt.edu> <jalemkul at vt.edu>
>> Date: Mon, 12 Sep 2011 22:35:28
>> To: <aiswarya.pawar at gmail.com> <aiswarya.pawar at gmail.com>; Discussion list for GROMACS users<gmx-users at gromacs.org> <gmx-users at gromacs.org>
>> Reply-To: jalemkul at vt.edu
>> Subject: Re: [gmx-users] g_dist error
>>
>>
>> aiswarya.pawar at gmail.com wrote:
>>
>>  Even if I specify an atom say 1277 atom number to find distance against the OW atoms. I get the same result of SOL-OW distance. Is it a bug cause even after specifying one atom from a protein why doesn't it give me the result for the SOL.
>>
>>
>>
>>  As was suggested several messages ago, please do NOT combine -o and -dist.  If
>> you want to measure a distance, use -o.  If you want g_dist to print a list of
>> atoms that satisfy some given criteria, use -dist, but not together.
>>
>> -Justin
>>
>>
>>  Thanks
>> Sent from my BlackBerry® on Reliance Mobile, India's No. 1 Network. Go for it!
>>
>> -----Original Message-----
>> From: "Justin A. Lemkul" <jalemkul at vt.edu> <jalemkul at vt.edu>
>> Sender: gmx-users-bounces at gromacs.org
>> Date: Mon, 12 Sep 2011 07:52:54
>> To: Discussion list for GROMACS users<gmx-users at gromacs.org> <gmx-users at gromacs.org>
>> Reply-To: jalemkul at vt.edu,
>> 	Discussion list for GROMACS users <gmx-users at gromacs.org> <gmx-users at gromacs.org>
>> Subject: Re: [gmx-users] g_dist error
>>
>>
>>
>> aiswarya pawar wrote:
>>
>>  hi Justin,
>>
>> As far i referred the OW,HW1 etc are water atoms so how can it be
>> distance between the SOL protein atoms, instead it is SOL water atoms.
>>
>>
>>  The printed distance indicates that there is a certain water molecule that is
>> just over 2 hydrogen bonding lengths away from your protein's backbone.  Sounds
>> normal to me.
>>
>> -Justin
>>
>>
>>  Thanks
>>
>> On Mon, Sep 12, 2011 at 4:49 PM, Justin A. Lemkul <jalemkul at vt.edu <mailto:jalemkul at vt.edu> <jalemkul at vt.edu>> wrote:
>>
>>
>>
>>     aiswarya pawar wrote:
>>
>>         Hi Users,
>>
>>         Am using g_dist to find the distance between water and protein.
>>         but my output has the values of SOL-water distance.
>>
>>         t: 1  136 SOL 2336 OW  0.772373 (nm)
>>
>>
>>     This is not a water-water distance, it is the output of the -dist
>>     option telling you that water molecule 136 has its OW atom at
>>     0.7723273 nm from whatever your reference group is.
>>
>>     -Justin
>>
>>     --
>>     ==============================__==========
>>
>>     Justin A. Lemkul
>>     Ph.D. Candidate
>>     ICTAS Doctoral Scholar
>>     MILES-IGERT Trainee
>>     Department of Biochemistry
>>     Virginia Tech
>>     Blacksburg, VA
>>     jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu> | (540) 231-9080
>>     http://www.bevanlab.biochem.__vt.edu/Pages/Personal/justin
>>     <http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin> <http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin>
>>
>>     ==============================__==========
>>     --
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