[gmx-users] problems with martinize.py

francesca vitalini francesca.vitalini11 at gmail.com
Mon Feb 20 13:57:16 CET 2012


I was using the one available from the tutorial but now I have
downloaded the new one and it gives me still an error message like

INFO       Chain termini will be charged
INFO       Residues at chain brakes will not be charged
INFO       Local elastic bonds will be used for extended regions.
INFO       Position restraints will be generated.
WARNING    Position restraints are only enabled if -DPOSRES is set in
the MDP file
INFO       Read input structure from file.
INFO       Input structure is a GRO file. Chains will be labeled consecutively.
Traceback (most recent call last):
  File "./martinize-1.0.py", line 2306, in <module>
    for title,atoms,box in frameIterator(inStream):
  File "./martinize-1.0.py", line 1183, in groFrameIterator
    atoms  = [groAtom(streamIterator.next())  for i in range(natoms)]
  File "./martinize-1.0.py", line 1170, in groAtom
    return (S(a[10:15]), S(a[5:10]),   I(a[:5]), " ",
10*F(a[20:28]),10*F(a[28:36]),10*F(a[36:44]))
ValueError: invalid literal for int() with base 10: '2.'

I have used the command line ./martinize-1.0.py -f npt_strip_wat.gro
-o frag_npt_cg_pdb.top -x frag_npt_cg_pdb.gro -v  -p backbone

were my input file is very simply

Protein in water
 2410
    1ALA      N    1   1.497   1.862   1.290  0.1206 -0.3069 -0.2102
    1ALA     H1    2   1.409   1.866   1.338  0.8590 -0.1748  1.1978
    1ALA     H2    3   1.547   1.948   1.296  2.0372 -1.4201  0.8359
    1ALA     H3    4   1.483   1.837   1.194  1.8924 -0.0993 -0.5621
    1ALA     CA    5   1.585   1.753   1.335 -0.1231 -0.2165  0.4955
    1ALA     CB    6   1.625   1.781   1.480  0.3554 -0.5405  0.4275
    1ALA      C    7   1.517   1.617   1.323  0.1234 -0.2807 -0.1917
    1ALA      O    8   1.407   1.624   1.268 -0.0033  0.4302  0.1378
    2GLU      N    9   1.566   1.515   1.393 -0.2655 -0.2542  0.1191
    2GLU      H   10   1.655   1.536   1.434  0.1561  0.1079 -0.9482
    2GLU     CA   11   1.501   1.384   1.402  0.3096 -0.5394  0.1823
    2GLU     CB   12   1.601   1.272   1.427  0.7527 -0.2033 -0.0775
    2GLU     CG   13   1.694   1.280   1.548 -0.0279  0.1789  0.5017
    2GLU     CD   14   1.779   1.405   1.571  0.4664 -0.1532  0.4921
    2GLU    OE1   15   1.882   1.412   1.500  0.2904 -0.0189  0.2469
    2GLU    OE2   16   1.740   1.489   1.654  0.2746  0.1001  0.1509
    2GLU      C   17   1.378   1.355   1.489  0.5085 -0.1385  0.5964
    2GLU      O   18   1.352   1.237   1.510  0.3837 -0.1792  0.2200
    3GLU      N   19   1.307   1.458   1.535 -0.1887 -0.4392  0.2009
    3GLU      H   20   1.315   1.547   1.489 -1.1961  0.1298  1.0835
    3GLU     CA   21   1.180   1.436   1.604 -0.3118 -0.2722  0.0307
    3GLU     CB   22   1.192   1.426   1.757 -0.3397  0.1329  0.0602
    3GLU      C   23   1.066   1.533   1.572 -0.3117 -0.4554 -0.5274
    3GLU     O1   24   1.072   1.606   1.471  0.0488  0.2651  0.0047
    3GLU     O2   25   0.965   1.542   1.646 -0.1745 -0.2606 -0.3621
   2.91477   2.91477   2.91477

Can you help me on that?
Thanks

2012/2/20 Tsjerk Wassenaar <tsjerkw at gmail.com>:
> Hi Francesca,
>
> Is this the latest version
> (http://md.chem.rug.nl/cgmartini/index.php/downloads/tools/204-martinize)?
> If it is, please send me the input file and I'll fix the bug. Note
> that the previous version that was available online was one used in a
> workshop, while the script was still in beta.
>
> Cheers,
>
> Tsjerk
>
>
> On Mon, Feb 20, 2012 at 10:43 AM, francesca vitalini
> <francesca.vitalini11 at gmail.com> wrote:
>> Hi all,
>> I'm trying to coarsegrain my structure using the script martinize.py
>> and using my gro file as inmput and the dssp file with the second
>> structure downloaded from http://swift.cmbi.ru.nl/gv/dssp using the
>> pdb structure as input, I get the following error message that I
>> really don't understand.
>>
>> INFO       Chain termini will be charged
>> INFO       Residues at chain brakes will not be charged
>> INFO       Local elastic bonds will be used for extended regions.
>> INFO       Position restraints will be generated.
>> WARNING    Position restraints are only enabled if -DPOSRES is set in
>> the MDP file
>> INFO       Read input structure from file.
>> INFO       Input structure is a GRO file. Chains will be labeled consecutively.
>> Traceback (most recent call last):
>>  File "./martinize.py", line 2037, in <module>
>>    for title,atoms,box in frameIterator(inStream):
>>  File "./martinize.py", line 1303, in groFrameIterator
>>    atoms  = [groAtom(streamIterator.next())  for i in range(natoms)]
>>  File "./martinize.py", line 1290, in groAtom
>>    return (S(a[10:15]), S(a[5:10]),   I(a[:5]), " ",
>> 10*F(a[20:28]),10*F(a[28:36]),10*F(a[36:44]))
>> ValueError: invalid literal for int() with base 10: '2.'
>>
>>  Can anyone help me on that?
>> Thanks
>>
>>
>>
>> --
>> Francesca
>> --
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>
>
>
> --
> Tsjerk A. Wassenaar, Ph.D.
>
> post-doctoral researcher
> Molecular Dynamics Group
> * Groningen Institute for Biomolecular Research and Biotechnology
> * Zernike Institute for Advanced Materials
> University of Groningen
> The Netherlands
> --
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-- 
Francesca Vitalini

PhD student at Computational Molecular Biology Group,
Department of Mathematics and Informatics, FU-Berlin
Arnimallee 6 14195 Berlin

vitalini at zedat.fu-berlin.de
francesca.vitalini at fu-berlin.de

+49 3083875776
+49 3083875412



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