[gmx-users] Cytochrom C

Peter C. Lai pcl at uab.edu
Fri Jan 6 20:18:34 CET 2012


He must be using an older version of Gromacs. 4.5.4 and lower don't have
ACE in charmm27.

On 2012-01-06 12:59:56PM -0600, Krzysztof Kuczera wrote:
> Here is the blocking group from 
> gromacs-4.5.5/share/top/charmm27.ff/aminoacids.rtp
> KK
> 
> [ ACE ]
>   [ atoms ]
>          CH3     CT3     -0.270  0
>          HH31    HA      0.090   1
>          HH32    HA      0.090   2
>          HH33    HA      0.090   3
>          C       C       0.510   4
>          O       O       -0.510  5
>   [ bonds ]
>          C       CH3
>          C       +N
>          CH3     HH31
>          CH3     HH32
>          CH3     HH33
>          O       C
>   [ impropers ]
>          C       CH3     +N      O
> 
> 
> On 1/6/12 12:40 PM, Peter C. Lai wrote:
> > Corrected bonds section (sorry been up all night)
> >
> >   [ ACE ]
> >    [ atoms ]
> >      CH3	    CT3    -0.27    0
> >      HH31     HA      0.09    1
> >      HH32     HA      0.09    2
> >      HH33     HA      0.09    3
> >      C        C       0.51    4
> >      O        O      -0.51    5
> >    [ bonds ]
> >      CH3    HH31
> >      CH3    HH32
> >      CH3    HH33
> >      CH3    C
> >      C      O
> >
> >   Surprisingly, an .hdb entry for ACE exists so you don't need to create one.
> > (and the .hdb entry uses HH3 as the base hydrogen name in ACE)
> >
> >>
> >> On 2012-01-06 12:23:49PM -0600, Peter C. Lai wrote:
> >>> Gromos96 53A6 has it.
> >>>
> >>> On 2012-01-06 01:18:38PM -0500, Dariush Mohammadyani wrote:
> >>>> I tried charmm27 too.
> >>>>
> >>>> Error:
> >>>> Residue 'ACE' not found in residue topology database
> >>>>
> >>>> I tried all forcefield in the list provided by "pdb2gmx", but non of them
> >>>> works.
> >>>>
> >>>> Dariush
> >>>>
> >>>>
> >>>> On Thu, Jan 5, 2012 at 1:42 PM, Robert Hamers<rjhamers at wisc.edu>  wrote:
> >>>>
> >>>>>     HEME is in the charmm27 force field.
> >>>>> bob h.
> >>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>> On 1/5/2012 12:00 PM, Dariush Mohammadyani wrote:
> >>>>>
> >>>>> Yes, I have PDB file (1HRC.pdb). However, when I try to use "pdb2gmx" I
> >>>>> get this error:
> >>>>>
> >>>>> Residue 'HEM' not found in residue topology database
> >>>>>
> >>>>> and HEM is Iron ion inside this protein. I do not know which forcefield is
> >>>>> proper to use. I also tried MARTINI force field according their website; I
> >>>>> used martinize.py script; Again I got error.
> >>>>>
> >>>>> Regards,
> >>>>> Dariush
> >>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>> On Thu, Jan 5, 2012 at 11:51 AM, Justin A. Lemkul<jalemkul at vt.edu>  wrote:
> >>>>>
> >>>>>>
> >>>>>> Dariush Mohammadyani wrote:
> >>>>>>
> >>>>>>> Hi all,
> >>>>>>>
> >>>>>>> Has anybody made initial configuration for Cytochrom C? Can it be shared
> >>>>>>> with me?
> >>>>>>>
> >>>>>>>
> >>>>>>   There are several in the PDB.
> >>>>>>
> >>>>>> -Justin
> >>>>>>
> >>>>>> --
> >>>>>> ========================================
> >>>>>>
> >>>>>> Justin A. Lemkul
> >>>>>> Ph.D. Candidate
> >>>>>> ICTAS Doctoral Scholar
> >>>>>> MILES-IGERT Trainee
> >>>>>> Department of Biochemistry
> >>>>>> Virginia Tech
> >>>>>> Blacksburg, VA
> >>>>>> jalemkul[at]vt.edu | (540) 231-9080
> >>>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> >>>>>>
> >>>>>> ========================================
> >>>>>>
> >>>>>> --
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> >>>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>>
> >>>>> --
> >>>>> Robert J. Hamers
> >>>>> Wisconsin Distinguished Professor
> >>>>> Univ. of Wisconsin-Madison	
> >>>>> 1101 University Avenue
> >>>>> Madison, WI 53706
> >>>>> Ph: 608-262-6371
> >>>>>   Web: http://hamers.chem.wisc.edu
> >>>>>
> >>>>>
> >>>>
> >>>> -- 
> >>>> Kind Regards,
> >>>> Dariush Mohammadyani
> >>>> Department of Structural Biology
> >>>> University of Pittsburgh School of Medicine
> >>>> Biomedical Science Tower 3
> >>>> 3501 Fifth Avenue
> >>>> Pittsburgh, PA 15261
> >>>> USA
> >>>> -- 
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> >>>
> >>> -- 
> >>> ==================================================================
> >>> Peter C. Lai			| University of Alabama-Birmingham
> >>> Programmer/Analyst		| KAUL 752A
> >>> Genetics, Div. of Research	| 705 South 20th Street
> >>> pcl at uab.edu			| Birmingham AL 35294-4461
> >>> (205) 690-0808			|
> >>> ==================================================================
> >>>
> >>> -- 
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> >> -- 
> >> ==================================================================
> >> Peter C. Lai			| University of Alabama-Birmingham
> >> Programmer/Analyst		| KAUL 752A
> >> Genetics, Div. of Research	| 705 South 20th Street
> >> pcl at uab.edu			| Birmingham AL 35294-4461
> >> (205) 690-0808			|
> >> ==================================================================
> >>
> >> -- 
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> 
> -- 
> Krzysztof Kuczera
> Professor
> Departments of Chemistry and Molecular Biosciences
> The University of Kansas
> 2010 Malott Hall
> Lawrence, KS 66045
> Tel: 785-864-5060 Fax: 785-864-5396 email: kkuczera at ku.edu
> http://oolung.chem.ku.edu/~kuczera/home.html
> 
> 
> 
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-- 
==================================================================
Peter C. Lai			| University of Alabama-Birmingham
Programmer/Analyst		| KAUL 752A
Genetics, Div. of Research	| 705 South 20th Street
pcl at uab.edu			| Birmingham AL 35294-4461
(205) 690-0808			|
==================================================================




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