[gmx-users] Normal Mode Analysis -- Expected Output

Bryan Roessler roessler at uab.edu
Thu Apr 11 17:57:19 CEST 2013


Hello,

I am running a normal mode analysis on a ~1500AA protein with the following
mdp parameters:

Log file opened on Tue Apr  9 09:55:00 2013
Host: uv1  pid: 128985  nodeid: 0  nnodes:  64
Gromacs version:    VERSION 4.6.1
Precision:          double
Memory model:       64 bit
MPI library:        MPI
OpenMP support:     disabled
GPU support:        disabled
invsqrt routine:    gmx_software_invsqrt(x)
CPU acceleration:   AVX_256
FFT library:        fftw-3.3.2-sse2
Large file support: enabled
RDTSCP usage:       enabled
Built on:           Fri Mar 15 09:20:59 CDT 2013
Built by:           asndcy at uv [CMAKE]
Build OS/arch:      Linux 3.0.58-0.6.6-default x86_64
Build CPU vendor:   GenuineIntel
Build CPU brand:    Intel(R) Xeon(R) CPU E5-2667 0 @ 2.90GHz
Build CPU family:   6   Model: 45   Stepping: 7
Build CPU features: aes apic avx clfsh cmov cx8 cx16 htt lahf_lm mmx msr
nonstop_tsc pcid pclmuldq pdcm pdpe1gb popcnt pse rdtscp sse2 sse3 sse4.1
sse4.2 ssse3 tdt x2apic
C compiler:         /opt/sgi/mpt/mpt-2.07/bin/mpicc GNU gcc (GCC) 4.7.2
C compiler flags:   -mavx   -Wextra -Wno-missing-field-initializers
-Wno-sign-compare -Wall -Wno-unused -Wunused-value -Wno-unknown-pragmas
-fomit-frame-pointer -funroll-all-loops -fexcess-precision=fast  -O3
-DNDEBUG


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        Contributions from Mark Abraham, Emile Apol, Rossen Apostolov,
           Herman J.C. Berendsen, Aldert van Buuren, Pär Bjelkmar,
     Rudi van Drunen, Anton Feenstra, Gerrit Groenhof, Christoph Junghans,
        Peter Kasson, Carsten Kutzner, Per Larsson, Pieter Meulenhoff,
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                Michael Shirts, Alfons Sijbers, Peter Tieleman,

               Berk Hess, David van der Spoel, and Erik Lindahl.

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         Copyright (c) 2001-2012,2013, The GROMACS development team at
        Uppsala University & The Royal Institute of Technology, Sweden.
            check out http://www.gromacs.org for more information.

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    :-)  /opt/asn/apps/gromacs_4.6.1/bin/mdrun_mpi_d (double precision)  (-:


++++ PLEASE READ AND CITE THE FOLLOWING REFERENCE ++++
B. Hess and C. Kutzner and D. van der Spoel and E. Lindahl
GROMACS 4: Algorithms for highly efficient, load-balanced, and scalable
molecular simulation
J. Chem. Theory Comput. 4 (2008) pp. 435-447
-------- -------- --- Thank You --- -------- --------


++++ PLEASE READ AND CITE THE FOLLOWING REFERENCE ++++
D. van der Spoel, E. Lindahl, B. Hess, G. Groenhof, A. E. Mark and H. J. C.
Berendsen
GROMACS: Fast, Flexible and Free
J. Comp. Chem. 26 (2005) pp. 1701-1719
-------- -------- --- Thank You --- -------- --------


++++ PLEASE READ AND CITE THE FOLLOWING REFERENCE ++++
E. Lindahl and B. Hess and D. van der Spoel
GROMACS 3.0: A package for molecular simulation and trajectory analysis
J. Mol. Mod. 7 (2001) pp. 306-317
-------- -------- --- Thank You --- -------- --------


++++ PLEASE READ AND CITE THE FOLLOWING REFERENCE ++++
H. J. C. Berendsen, D. van der Spoel and R. van Drunen
GROMACS: A message-passing parallel molecular dynamics implementation
Comp. Phys. Comm. 91 (1995) pp. 43-56
-------- -------- --- Thank You --- -------- --------


Changing rlist from 1.47 to 1.4 for non-bonded 4x4 atom kernels

Input Parameters:
   integrator           = nm
   nsteps               = 100000
   init-step            = 0
   cutoff-scheme        = Verlet
   ns_type              = Grid
   nstlist              = 10
   ndelta               = 2
   nstcomm              = 100
   comm-mode            = Linear
   nstlog               = 1000
   nstxout              = 500
   nstvout              = 500
   nstfout              = 500
   nstcalcenergy        = 100
   nstenergy            = 500
   nstxtcout            = 0
   init-t               = 0
   delta-t              = 0.002
   xtcprec              = 1000
   fourierspacing       = 0.12
   nkx                  = 160
   nky                  = 160
   nkz                  = 216
   pme-order            = 4
   ewald-rtol           = 1e-05
   ewald-geometry       = 0
   epsilon-surface      = 0
   optimize-fft         = TRUE
   ePBC                 = xyz
   bPeriodicMols        = FALSE
   bContinuation        = FALSE
   bShakeSOR            = FALSE
   etc                  = No
   bPrintNHChains       = FALSE
   nsttcouple           = -1
   epc                  = No
   epctype              = Isotropic
   nstpcouple           = -1
   tau-p                = 1
   ref-p (3x3):
      ref-p[    0]={ 1.00000e+00,  0.00000e+00,  0.00000e+00}
      ref-p[    1]={ 0.00000e+00,  1.00000e+00,  0.00000e+00}
      ref-p[    2]={ 0.00000e+00,  0.00000e+00,  1.00000e+00}
   compress (3x3):
      compress[    0]={ 4.50000e-05,  0.00000e+00,  0.00000e+00}
      compress[    1]={ 0.00000e+00,  4.50000e-05,  0.00000e+00}
      compress[    2]={ 0.00000e+00,  0.00000e+00,  4.50000e-05}
   refcoord-scaling     = No
   posres-com (3):
      posres-com[0]= 0.00000e+00
      posres-com[1]= 0.00000e+00
      posres-com[2]= 0.00000e+00
   posres-comB (3):
      posres-comB[0]= 0.00000e+00
      posres-comB[1]= 0.00000e+00
      posres-comB[2]= 0.00000e+00
   verlet-buffer-drift  = 0.005
   rlist                = 1.4
   rlistlong            = 1.4
   nstcalclr            = 10
   rtpi                 = 0.05
   coulombtype          = PME
   coulomb-modifier     = Potential-shift
   rcoulomb-switch      = 1.2
   rcoulomb             = 1.4
   vdwtype              = Cut-off
   vdw-modifier         = Potential-shift
   rvdw-switch          = 1.2
   rvdw                 = 1.4
   epsilon-r            = 1
   epsilon-rf           = inf
   tabext               = 1
   implicit-solvent     = No
   gb-algorithm         = Still
   gb-epsilon-solvent   = 80
   nstgbradii           = 1
   rgbradii             = 1
   gb-saltconc          = 0
   gb-obc-alpha         = 1
   gb-obc-beta          = 0.8
   gb-obc-gamma         = 4.85
   gb-dielectric-offset = 0.009
   sa-algorithm         = Ace-approximation
   sa-surface-tension   = 2.05016
   DispCorr             = No
   bSimTemp             = FALSE
   free-energy          = no
   nwall                = 0
   wall-type            = 9-3
   wall-atomtype[0]     = -1
   wall-atomtype[1]     = -1
   wall-density[0]      = 0
   wall-density[1]      = 0
   wall-ewald-zfac      = 3
   pull                 = no
   rotation             = FALSE
   disre                = No
   disre-weighting      = Conservative
   disre-mixed          = FALSE
   dr-fc                = 1000
   dr-tau               = 0
   nstdisreout          = 100
   orires-fc            = 0
   orires-tau           = 0
   nstorireout          = 100
   dihre-fc             = 0
   em-stepsize          = 0.01
   em-tol               = 10
   niter                = 20
   fc-stepsize          = 0
   nstcgsteep           = 1000
   nbfgscorr            = 10
   ConstAlg             = Lincs
   shake-tol            = 0.0001
   lincs-order          = 4
   lincs-warnangle      = 30
   lincs-iter           = 1
   bd-fric              = 0
   ld-seed              = 1993
   cos-accel            = 0
   deform (3x3):
      deform[    0]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
      deform[    1]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
      deform[    2]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
   adress               = FALSE
   userint1             = 0
   userint2             = 0
   userint3             = 0
   userint4             = 0
   userreal1            = 0
   userreal2            = 0
   userreal3            = 0
   userreal4            = 0
grpopts:
   nrdf:       71907
   ref-t:           0
   tau-t:           0
anneal:          No
ann-npoints:           0
   acc:           0           0           0
   nfreeze:           N           N           N
   energygrp-flags[  0]: 0
   efield-x:
      n = 0
   efield-xt:
      n = 0
   efield-y:
      n = 0
   efield-yt:
      n = 0
   efield-z:
      n = 0
   efield-zt:
      n = 0
   bQMMM                = FALSE
   QMconstraints        = 0
   QMMMscheme           = 0
   scalefactor          = 1
qm-opts:
   ngQM                 = 0

Non-default thread affinity set, disabling internal thread affinity
Using 64 MPI processes

Detecting CPU-specific acceleration.
Present hardware specification:
Vendor: GenuineIntel
Brand:  Intel(R) Xeon(R) CPU E5-4640 0 @ 2.40GHz
Family:  6  Model: 45  Stepping:  7
Features: aes apic avx clfsh cmov cx8 cx16 htt lahf_lm mmx msr nonstop_tsc
pcid pclmuldq pdcm pdpe1gb popcnt pse rdtscp sse2 sse3 sse4.1 sse4.2 ssse3
tdt x2apic
Acceleration most likely to fit this hardware: AVX_256
Acceleration selected at GROMACS compile time: AVX_256

Will do PME sum in reciprocal space.

++++ PLEASE READ AND CITE THE FOLLOWING REFERENCE ++++
U. Essmann, L. Perera, M. L. Berkowitz, T. Darden, H. Lee and L. G.
Pedersen
A smooth particle mesh Ewald method
J. Chem. Phys. 103 (1995) pp. 8577-8592
-------- -------- --- Thank You --- -------- --------

Will do ordinary reciprocal space Ewald sum.
Using a Gaussian width (1/beta) of 0.448228 nm for Ewald
Cut-off's:   NS: 1.4   Coulomb: 1.4   LJ: 1.4
System total charge: 19.000
Generated table with 4800 data points for Ewald.
Tabscale = 2000 points/nm
Generated table with 4800 data points for LJ6.
Tabscale = 2000 points/nm
Generated table with 4800 data points for LJ12.
Tabscale = 2000 points/nm
Generated table with 4800 data points for 1-4 COUL.
Tabscale = 2000 points/nm
Generated table with 4800 data points for 1-4 LJ6.
Tabscale = 2000 points/nm
Generated table with 4800 data points for 1-4 LJ12.
Tabscale = 2000 points/nm

Using AVX-256 4x4 non-bonded kernels

Using Lorentz-Berthelot Lennard-Jones combination rule

Potential shift: LJ r^-12: 0.018 r^-6 0.133, Ewald 1.000e-05
Initialized non-bonded Ewald correction tables, spacing: 7.81e-04 size: 3076

Removing pbc first time
Initiating Normal Mode Analysis
Started Normal Mode Analysis on node 0 Sun Apr  7 09:55:01 2013


However, my NMA has been running for about 4 days on 64 Xeon nodes with
120GB available memory and GROMACS has not generated any output.

What should I expect to see, and how would I adjust my mdp parameters to
increase the frequency of output of the normal-mode analysis? How long
would a run like this be expected to take?

Thank you,

Bryan



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