[gmx-users] Abot Free energy methods in gromacs

[vidhya sankar]

Dear Justin ,
                      Thank you for your Previous reply.
I am Doing Simulation of assembly of  Cyclic Petide in POPC membrane
For that I need to calculate Free energy for Stability of Assembly.
You suggested the Umbrella sampling 

But Aprt from that i wish to do
Free energy perturbation method or MM-PBSA method 
Is the Afore said method is suitable or Not . to study of assembly of Cyclic Peptide in POPC membrane 

If it is suitable then Is ther is any tutorial for those methods in Gromacs? With Installation of MM-PBSa interface  with Gromacs 

Thanks in Advance