[gmx-users] adding a new residue into an existing force field?

marzieh dehghan dehghanmarzieh at gmail.com
Fri Dec 5 08:59:06 CET 2014


Hi
according to the previous email, since I want to keep covalent bond during
the MD using gromacs, you said me, I should add a new residue into an
existing force field
<http://www.gromacs.org/Documentation/Terminology/Force_Fields>, but I have
two questions?

1- based on the gromacs website , I can copy residuetypes.dat and
spcbond.dat in the working directory. is it possible to copy
" amino acid.rtp, aminoacid.hdb, atomtypes.atp" in the working directory?

2- can I use one of the  output file of prodrug server (the gromacs
topology) as aminoacid.rtp?
(copy at the end of aminoacid.rtp or it use as an independent file?)

[ moleculetype ]
; Name nrexcl
GLC            3
[ atoms ]
;   nr      type  resnr resid  atom  cgnr   charge     mass
     1        OM     1  UNK     OAF     1   -0.322  15.9994
     2         P     1  UNK     PAE     1    1.063  30.9738
     3        OM     1  UNK     OAG     1   -0.322  15.9994
     4        OM     1  UNK     OAH     1   -0.322  15.9994
     5        OA     1  UNK     OAD     1   -0.097  15.9994
     6       CH2     1  UNK     CAC     2   -0.044  14.0270
     7         C     1  UNK     CAB     2    0.184  12.0110
     8        OA     1  UNK     OAA     2   -0.149  15.9994
     9         H     1  UNK     HAA     2   -0.013   1.0080
    10         C     1  UNK     CAI     2    0.184  12.0110
    11        OA     1  UNK     OAJ     2   -0.149  15.9994
    12         H     1  UNK     HAJ     2   -0.013   1.0080
    13         C     1  UNK     CAK     3    0.218  12.0110
    14        OA     1  UNK     OAL     3   -0.126  15.9994
    15         H     1  UNK     HAL     3   -0.011   1.0080
    16         C     1  UNK     CAM     3    0.218  12.0110
    17        OA     1  UNK     OAN     3   -0.126  15.9994
    18         H     1  UNK     HAN     3   -0.011   1.0080
    19       CH2     1  UNK     CAO     3   -0.025  14.0270
    20        OA     1  UNK     OAP     3   -0.126  15.9994
    21         H     1  UNK     HAQ     3   -0.011   1.0080
[ bonds ]
; ai  aj  fu    c0, c1, ...
   2   1   2    0.148   8600000.0    0.148   8600000.0 ;   PAE  OAF
   2   3   2    0.148   8600000.0    0.148   8600000.0 ;   PAE  OAG
   2   4   2    0.148   8600000.0    0.148   8600000.0 ;   PAE  OAH
   2   5   2    0.161   4840000.0    0.161   4840000.0 ;   PAE  OAD
   6   5   2    0.143   8180000.0    0.143   8180000.0 ;   CAC  OAD
   7   6   2    0.153   7150000.0    0.153   7150000.0 ;   CAB  CAC
   7   8   2    0.136  10200000.0    0.136  10200000.0 ;   CAB  OAA
   7  10   2    0.153   7150000.0    0.153   7150000.0 ;   CAB  CAI
   8   9   2    0.100  15700000.0    0.100  15700000.0 ;   OAA  HAA
  10  11   2    0.136  10200000.0    0.136  10200000.0 ;   CAI  OAJ
  10  13   2    0.153   7150000.0    0.153   7150000.0 ;   CAI  CAK
  11  12   2    0.100  15700000.0    0.100  15700000.0 ;   OAJ  HAJ
  13  14   2    0.136  10200000.0    0.136  10200000.0 ;   CAK  OAL
  13  16   2    0.153   7150000.0    0.153   7150000.0 ;   CAK  CAM
  14  15   2    0.100  15700000.0    0.100  15700000.0 ;   OAL  HAL
  16  17   2    0.136  10200000.0    0.136  10200000.0 ;   CAM  OAN
  16  19   2    0.153   7150000.0    0.153   7150000.0 ;   CAM  CAO
  17  18   2    0.100  15700000.0    0.100  15700000.0 ;   OAN  HAN
  19  20   2    0.143   8180000.0    0.143   8180000.0 ;   CAO  OAP
  20  21   2    0.100  15700000.0    0.100  15700000.0 ;   OAP  HAQ
[ pairs ]
; ai  aj  fu    c0, c1, ...
   1   6   1                                           ;   OAF  CAC
   2   7   1                                           ;   PAE  CAB
   3   6   1                                           ;   OAG  CAC
   4   6   1                                           ;   OAH  CAC
   5   8   1                                           ;   OAD  OAA
   5  10   1                                           ;   OAD  CAI
   6   9   1                                           ;   CAC  HAA
   6  11   1                                           ;   CAC  OAJ
   6  13   1                                           ;   CAC  CAK
   7  12   1                                           ;   CAB  HAJ
   7  14   1                                           ;   CAB  OAL
   7  16   1                                           ;   CAB  CAM
   8  11   1                                           ;   OAA  OAJ
   8  13   1                                           ;   OAA  CAK
   9  10   1                                           ;   HAA  CAI
  10  15   1                                           ;   CAI  HAL
  10  17   1                                           ;   CAI  OAN
  10  19   1                                           ;   CAI  CAO
  11  14   1                                           ;   OAJ  OAL
  11  16   1                                           ;   OAJ  CAM
  12  13   1                                           ;   HAJ  CAK
  13  18   1                                           ;   CAK  HAN
  13  20   1                                           ;   CAK  OAP
  14  17   1                                           ;   OAL  OAN
  14  19   1                                           ;   OAL  CAO
  15  16   1                                           ;   HAL  CAM
  16  21   1                                           ;   CAM  HAQ
  17  20   1                                           ;   OAN  OAP
  18  19   1                                           ;   HAN  CAO
[ angles ]
; ai  aj  ak  fu    c0, c1, ...
   1   2   3   2    120.0       780.0    120.0       780.0 ;   OAF  PAE
OAG
   1   2   4   2    120.0       780.0    120.0       780.0 ;   OAF  PAE
OAH
   1   2   5   2    109.6       450.0    109.6       450.0 ;   OAF  PAE
OAD
   3   2   4   2    120.0       780.0    120.0       780.0 ;   OAG  PAE
OAH
   3   2   5   2    109.6       450.0    109.6       450.0 ;   OAG  PAE
OAD
   4   2   5   2    109.6       450.0    109.6       450.0 ;   OAH  PAE
OAD
   2   5   6   2    120.0       530.0    120.0       530.0 ;   PAE  OAD
CAC
   5   6   7   2    109.5       520.0    109.5       520.0 ;   OAD  CAC
CAB
   6   7   8   2    115.0       610.0    115.0       610.0 ;   CAC  CAB
OAA
   6   7  10   2    115.0       610.0    115.0       610.0 ;   CAC  CAB
CAI
   8   7  10   2    115.0       610.0    115.0       610.0 ;   OAA  CAB
CAI
   7   8   9   2    109.5       450.0    109.5       450.0 ;   CAB  OAA
HAA
   7  10  11   2    115.0       610.0    115.0       610.0 ;   CAB  CAI
OAJ
   7  10  13   2    115.0       610.0    115.0       610.0 ;   CAB  CAI
CAK
  11  10  13   2    115.0       610.0    115.0       610.0 ;   OAJ  CAI
CAK
  10  11  12   2    109.5       450.0    109.5       450.0 ;   CAI  OAJ
HAJ
  10  13  14   2    115.0       610.0    115.0       610.0 ;   CAI  CAK
OAL
  10  13  16   2    115.0       610.0    115.0       610.0 ;   CAI  CAK
CAM
  14  13  16   2    115.0       610.0    115.0       610.0 ;   OAL  CAK
CAM
  13  14  15   2    109.5       450.0    109.5       450.0 ;   CAK  OAL
HAL
  13  16  17   2    115.0       610.0    115.0       610.0 ;   CAK  CAM
OAN
  13  16  19   2    115.0       610.0    115.0       610.0 ;   CAK  CAM
CAO
  17  16  19   2    115.0       610.0    115.0       610.0 ;   OAN  CAM
CAO
  16  17  18   2    109.5       450.0    109.5       450.0 ;   CAM  OAN
HAN
  16  19  20   2    109.5       520.0    109.5       520.0 ;   CAM  CAO
OAP
  19  20  21   2    109.5       450.0    109.5       450.0 ;   CAO  OAP
HAQ
[ dihedrals ]
; ai  aj  ak  al  fu    c0, c1, m, ...
   2   1   3   4   2     35.3  334.8       35.3  334.8   ; imp   PAE  OAF
OAG  OAH
   7   6   8  10   2      0.0  167.4        0.0  167.4   ; imp   CAB  CAC
OAA  CAI
  10  13  11   7   2      0.0  167.4        0.0  167.4   ; imp   CAI  CAK
OAJ  CAB
  13  16  14  10   2      0.0  167.4        0.0  167.4   ; imp   CAK  CAM
OAL  CAI
  16  19  17  13   2      0.0  167.4        0.0  167.4   ; imp   CAM  CAO
OAN  CAK
   6   5   2   1   1      0.0    3.1 2      0.0    3.1 2 ; dih   CAC  OAD
PAE  OAF
   7   6   5   2   1      0.0    1.3 3      0.0    1.3 3 ; dih   CAB  CAC
OAD  PAE
   5   6   7  10   1      0.0    1.0 6      0.0    1.0 6 ; dih   OAD  CAC
CAB  CAI
   6   7   8   9   1    180.0   16.7 2    180.0   16.7 2 ; dih   CAC  CAB
OAA  HAA
  13  10   7   6   1    180.0    5.9 2    180.0    5.9 2 ; dih   CAK  CAI
CAB  CAC
   7  10  11  12   1    180.0   16.7 2    180.0   16.7 2 ; dih   CAB  CAI
OAJ  HAJ
  16  13  10   7   1    180.0    5.9 2    180.0    5.9 2 ; dih   CAM  CAK
CAI  CAB
  10  13  14  15   1    180.0   16.7 2    180.0   16.7 2 ; dih   CAI  CAK
OAL  HAL
  19  16  13  10   1    180.0    5.9 2    180.0    5.9 2 ; dih   CAO  CAM
CAK  CAI
  13  16  17  18   1    180.0   16.7 2    180.0   16.7 2 ; dih   CAK  CAM
OAN  HAN
  20  19  16  13   1      0.0    1.0 6      0.0    1.0 6 ; dih   OAP  CAO
CAM  CAK
  16  19  20  21   1      0.0    1.3 3      0.0    1.3 3 ; dih   CAM  CAO
OAP  HAQ


thanks a lot

-- 




*Marzieh DehghanPhD Candidate of BiochemistryInstitute of biochemistry and
Biophysics (IBB)University of Tehran, Tehran- Iran.*


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