[gmx-users] CHARMM-GUI files give error in Gromacs

Justin Lemkul jalemkul at vt.edu
Fri Sep 2 16:31:13 CEST 2016



On 9/2/16 10:28 AM, gozde ergin wrote:
> Dear all,
>
> I used CHARMM-Gui and prepared the .pdb, .itp and .top file for cis pionic acid.
> After that I used genbox command of gromacs and add some water molecules.
> When I try to run I get 100 Errors saying :
>
> No default bond types, No default U-B types, No default Proper Dih. types, No default Improper Dih. types.
>
> All these errors are related to .itp file. However .itp files seems correct:
>
> Any help would be appreciated. Thanks.
>
>

CHARMM-GUI provides a self-contained force field file that will have everything 
you need.  It is a subset of the full CHARMM force field plus any parameters 
that are newly introduced by your non-standard molecule.  Are you using only 
files directly from CHARMM-GUI, without modification, or are you trying to do 
something else?

-Justin

> ;;
> ;; Generated by CHARMM-GUI (http://www.charmm-gui.org) v1.7
> ;;
> ;; psf2itp_mol.py
> ;;
> ;; Correspondance:
> ;; j712l362 at ku.edu or wonpil at ku.edu
> ;;
> ;; GROMACS topology file for LIG
> ;;
>
>
> [ moleculetype ]
> ; name	nrexcl
> LIG	     3
>
> [ atoms ]
> ; nr	type	resnr	residu	atom	cgnr	charge	mass
>      1     CG3C41      1      LIG     C2      1     -0.087    12.0110   ; qtot -0.087
>      2     CG3C41      1      LIG     C1      2     -0.046    12.0110   ; qtot -0.133
>      3       HGA1      1      LIG     H1      3      0.090     1.0080   ; qtot -0.043
>      4     CG3C41      1      LIG     C3      4     -0.038    12.0110   ; qtot -0.081
>      5      CG2O5      1      LIG     C8      5      0.388    12.0110   ; qtot  0.307
>      6       HGA1      1      LIG     H2      6      0.090     1.0080   ; qtot  0.397
>      7      OG2D3      1      LIG     O1      7     -0.481    15.9994   ; qtot -0.084
>      8      CG331      1      LIG    C10      8     -0.229    12.0110   ; qtot -0.313
>      9       HGA3      1      LIG    H14      9      0.090     1.0080   ; qtot -0.223
>     10       HGA3      1      LIG    H15     10      0.090     1.0080   ; qtot -0.133
>     11       HGA3      1      LIG    H13     11      0.090     1.0080   ; qtot -0.043
>     12     CG3C41      1      LIG     C4     12     -0.178    12.0110   ; qtot -0.221
>     13       HGA2      1      LIG     H4     13      0.090     1.0080   ; qtot -0.131
>     14       HGA2      1      LIG     H3     14      0.090     1.0080   ; qtot -0.041
>     15      CG331      1      LIG     C6     15     -0.248    12.0110   ; qtot -0.289
>     16       HGA3      1      LIG    H10     16      0.090     1.0080   ; qtot -0.199
>     17       HGA3      1      LIG     H9     17      0.090     1.0080   ; qtot -0.109
>     18       HGA3      1      LIG     H8     18      0.090     1.0080   ; qtot -0.019
>     19      CG331      1      LIG     C5     19     -0.248    12.0110   ; qtot -0.267
>     20       HGA3      1      LIG     H6     20      0.090     1.0080   ; qtot -0.177
>     21       HGA3      1      LIG     H7     21      0.090     1.0080   ; qtot -0.087
>     22       HGA3      1      LIG     H5     22      0.090     1.0080   ; qtot  0.003
>     23      CG321      1      LIG     C7     23     -0.207    12.0110   ; qtot -0.204
>     24      CG2O2      1      LIG     C9     24      0.803    12.0110   ; qtot  0.599
>     25       HGA2      1      LIG    H12     25      0.090     1.0080   ; qtot  0.689
>     26       HGA2      1      LIG    H11     26      0.090     1.0080   ; qtot  0.779
>     27      OG2D1      1      LIG     O3     27     -0.576    15.9994   ; qtot  0.203
>     28      OG311      1      LIG     O2     28     -0.632    15.9994   ; qtot -0.429
>     29       HGP1      1      LIG    H16     29      0.429     1.0080   ; qtot  0.000
>
> [ bonds ]
> ; ai	aj	funct	b0	Kb
>     1     2     1
>     1     3     1
>     1    12     1
>     1    23     1
>     2     4     1
>     2    15     1
>     2    19     1
>     4     5     1
>     4     6     1
>     4    12     1
>     5     7     1
>     5     8     1
>     8     9     1
>     8    10     1
>     8    11     1
>    12    13     1
>    12    14     1
>    15    16     1
>    15    17     1
>    15    18     1
>    19    20     1
>    19    21     1
>    19    22     1
>    23    24     1
>    23    25     1
>    23    26     1
>    24    27     1
>    24    28     1
>    28    29     1
>
> [ pairs ]
> ; ai	aj	funct	c6	c12
>     1     2     1
>     1     5     1
>     1     6     1
>     1    12     1
>     1    16     1
>     1    17     1
>     1    18     1
>     1    20     1
>     1    21     1
>     1    22     1
>     1    27     1
>     1    28     1
>     2     4     1
>     2     7     1
>     2     8     1
>     2    13     1
>     2    14     1
>     2    24     1
>     2    25     1
>     2    26     1
>     3     4     1
>     3    13     1
>     3    14     1
>     3    15     1
>     3    19     1
>     3    24     1
>     3    25     1
>     3    26     1
>     4     9     1
>     4    10     1
>     4    11     1
>     4    12     1
>     4    16     1
>     4    17     1
>     4    18     1
>     4    20     1
>     4    21     1
>     4    22     1
>     4    23     1
>     5    13     1
>     5    14     1
>     5    15     1
>     5    19     1
>     6     7     1
>     6     8     1
>     6    13     1
>     6    14     1
>     6    15     1
>     6    19     1
>     7     9     1
>     7    10     1
>     7    11     1
>     7    12     1
>     8    12     1
>    12    15     1
>    12    19     1
>    12    24     1
>    12    25     1
>    12    26     1
>    13    23     1
>    14    23     1
>    15    20     1
>    15    21     1
>    15    22     1
>    15    23     1
>    16    19     1
>    17    19     1
>    18    19     1
>    19    23     1
>    23    29     1
>    25    27     1
>    25    28     1
>    26    27     1
>    26    28     1
>    27    29     1
>
> [ angles ]
> ; ai	aj	ak	funct	th0	cth	S0	Kub
>     2     1     3     5
>     2     1    12     5
>     2     1    23     5
>     3     1    12     5
>     3     1    23     5
>    12     1    23     5
>     1     2     4     5
>     1     2    15     5
>     1     2    19     5
>     4     2    15     5
>     4     2    19     5
>    15     2    19     5
>     2     4     5     5
>     2     4     6     5
>     2     4    12     5
>     5     4     6     5
>     5     4    12     5
>     6     4    12     5
>     4     5     7     5
>     4     5     8     5
>     7     5     8     5
>     5     8     9     5
>     5     8    10     5
>     5     8    11     5
>     9     8    10     5
>     9     8    11     5
>    10     8    11     5
>     1    12     4     5
>     1    12    13     5
>     1    12    14     5
>     4    12    13     5
>     4    12    14     5
>    13    12    14     5
>     2    15    16     5
>     2    15    17     5
>     2    15    18     5
>    16    15    17     5
>    16    15    18     5
>    17    15    18     5
>     2    19    20     5
>     2    19    21     5
>     2    19    22     5
>    20    19    21     5
>    20    19    22     5
>    21    19    22     5
>     1    23    24     5
>     1    23    25     5
>     1    23    26     5
>    24    23    25     5
>    24    23    26     5
>    25    23    26     5
>    23    24    27     5
>    23    24    28     5
>    27    24    28     5
>    24    28    29     5
>
> [ dihedrals ]
> ; ai	aj	ak	al	funct	phi0	cp	mult
>     3     1     2     4     9
>     3     1     2    15     9
>     3     1     2    19     9
>    12     1     2    15     9
>    12     1     2    19     9
>     2     1    12     4     9
>     2     1    12    13     9
>     2     1    12    14     9
>     3     1    12     4     9
>     3     1    12    13     9
>     3     1    12    14     9
>     2     1    23    24     9
>     2     1    23    25     9
>     2     1    23    26     9
>     3     1    23    24     9
>     3     1    23    25     9
>     3     1    23    26     9
>    12     1    23    24     9
>    12     1    23    25     9
>    12     1    23    26     9
>     4     2     1    12     9
>     4     2     1    23     9
>    15     2     1    23     9
>    19     2     1    23     9
>     1     2     4     5     9
>     1     2     4     6     9
>     1     2     4    12     9
>     1     2    15    16     9
>     1     2    15    17     9
>     1     2    15    18     9
>     4     2    15    16     9
>     4     2    15    17     9
>     4     2    15    18     9
>     1     2    19    20     9
>     1     2    19    21     9
>     1     2    19    22     9
>     4     2    19    20     9
>     4     2    19    21     9
>     4     2    19    22     9
>    15     2    19    20     9
>    15     2    19    21     9
>    15     2    19    22     9
>     5     4     2    15     9
>     5     4     2    19     9
>     6     4     2    15     9
>     6     4     2    19     9
>    12     4     2    15     9
>    12     4     2    19     9
>     2     4     5     7     9
>     2     4     5     8     9
>     6     4     5     7     9
>     6     4     5     8     9
>     2     4    12    13     9
>     2     4    12    14     9
>     5     4    12    13     9
>     5     4    12    14     9
>     6     4    12    13     9
>     6     4    12    14     9
>     7     5     4    12     9
>     8     5     4    12     9
>     4     5     8     9     9
>     4     5     8    10     9
>     4     5     8    11     9
>     7     5     8     9     9
>     7     5     8    10     9
>     7     5     8    11     9
>     4    12     1    23     9
>    13    12     1    23     9
>    14    12     1    23     9
>     1    12     4     2     9
>     1    12     4     5     9
>     1    12     4     6     9
>    16    15     2    19     9
>    17    15     2    19     9
>    18    15     2    19     9
>     1    23    24    27     9
>     1    23    24    28     9
>    25    23    24    27     9
>    25    23    24    28     9
>    26    23    24    27     9
>    26    23    24    28     9
>    23    24    28    29     9
>    27    24    28    29     9
>
> [ dihedrals ]
> ; ai	aj	ak	al	funct	q0	cq
>     5     8     4     7     2
>    24    23    27    28     2
>

-- 
==================================================

Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

jalemkul at outerbanks.umaryland.edu | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

==================================================


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