[gmx-users] HEME issue

Mehreen Jan mehreen_jan1990 at yahoo.com
Thu Feb 9 11:54:54 CET 2017


hello every one 
i want to simulate HEME using gromacs 5.0.7 ff43A1 . i read some where HEME is already present in almost all gromacs FF. when i run Pdb2gmx.......HEME.gro  i got error Residue 'HEM' not found in residue topology database. i compare my PDB with rtp file ... i am shocked to see that my PDB HEM and rtp [HEME] are completely different. i have no idea what to do and how to do??
 i have no access to CHARMM-GUI, SwissParam give me error and ATB didnot provide login password. if some one have HEM modified topology kindly upload it or send. 

 my HEME:

HETATM    1 FE   HEM C1542       7.320  80.715  -5.955  1.00 25.63          Fe
HETATM    2  CHA HEM C1542       7.828  77.591  -5.047  1.00 23.39           C
HETATM    3  CHB HEM C1542       6.501  81.578  -2.733  1.00 23.47           C
HETATM    4  CHC HEM C1542       6.798  84.039  -6.886  1.00 22.00           C
HETATM    5  CHD HEM C1542       7.415  79.872  -9.275  1.00 20.35           C
HETATM    6  NA  HEM C1542       7.232  79.764  -4.194  1.00 25.04           N
HETATM    7  C1A HEM C1542       7.469  78.413  -3.980  1.00 25.77           C
HETATM    8  C2A HEM C1542       7.236  78.109  -2.602  1.00 26.75           C
HETATM    9  C3A HEM C1542       6.841  79.239  -1.971  1.00 26.16           C
HETATM   10  C4A HEM C1542       6.845  80.286  -2.956  1.00 24.77           C
HETATM   11  CMA HEM C1542       6.512  79.509  -0.487  1.00 23.24           C
HETATM   12  CAA HEM C1542       7.389  76.695  -1.990  1.00 30.57           C
HETATM   13  CBA HEM C1542       8.749  76.200  -1.503  1.00 34.82           C
HETATM   14  CGA HEM C1542       8.827  74.825  -0.843  1.00 37.87           C
HETATM   15  O1A HEM C1542       7.934  73.913  -0.680  1.00 39.74           O
HETATM   16  O2A HEM C1542      10.021  74.626  -0.423  1.00 40.42           O
HETATM   17  NB  HEM C1542       6.751  82.455  -4.998  1.00 23.10           N
HETATM   18  C1B HEM C1542       6.460  82.611  -3.660  1.00 22.37           C
HETATM   19  C2B HEM C1542       6.131  83.997  -3.367  1.00 21.40           C
HETATM   20  C3B HEM C1542       6.235  84.681  -4.510  1.00 21.71           C
HETATM   21  C4B HEM C1542       6.614  83.714  -5.544  1.00 21.85           C
HETATM   22  CMB HEM C1542       5.830  84.540  -1.947  1.00 20.77           C
HETATM   23  CAB HEM C1542       6.009  86.177  -4.817  1.00 20.85           C
HETATM   24  CBB HEM C1542       4.894  86.822  -4.391  1.00 21.93           C
HETATM   25  NC  HEM C1542       7.180  81.751  -7.717  1.00 23.07           N
HETATM   26  C1C HEM C1542       6.859  83.099  -7.910  1.00 21.16           C
HETATM   27  C2C HEM C1542       6.559  83.360  -9.301  1.00 19.55           C
HETATM   28  C3C HEM C1542       6.724  82.197  -9.967  1.00 19.24           C
HETATM   29  C4C HEM C1542       7.132  81.190  -9.023  1.00 21.12           C
HETATM   30  CMC HEM C1542       6.119  84.748  -9.815  1.00 17.45           C
HETATM   31  CAC HEM C1542       6.506  81.891 -11.455  1.00 17.62           C
HETATM   32  CBC HEM C1542       5.616  82.527 -12.236  1.00 18.11           C
HETATM   33  ND  HEM C1542       7.593  79.010  -6.981  1.00 22.41           N
HETATM   34  C1D HEM C1542       7.635  78.876  -8.352  1.00 21.08           C
HETATM   35  C2D HEM C1542       7.835  77.476  -8.654  1.00 21.66           C
HETATM   36  C3D HEM C1542       7.913  76.830  -7.505  1.00 21.01           C
HETATM   37  C4D HEM C1542       7.763  77.760  -6.423  1.00 22.07           C
HETATM   38  CMD HEM C1542       7.919  76.887 -10.102  1.00 20.29           C
HETATM   39  CAD HEM C1542       8.131  75.321  -7.321  1.00 20.98           C
HETATM   40  CBD HEM C1542       6.723  74.752  -7.027  1.00 23.17           C
HETATM   41  CGD HEM C1542       6.768  73.298  -6.794  1.00 24.91           C
HETATM   42  O1D HEM C1542       6.242  72.753  -5.808  1.00 29.44           O
HETATM   43  O2D HEM C1542       7.406  72.657  -7.630  1.00 26.84           O
HETATM   44  HHA HEM C1542       8.236  76.638  -4.744  1.00  0.00           H
HETATM   45  HHB HEM C1542       6.228  81.833  -1.720  1.00  0.00           H
HETATM   46  HHC HEM C1542       6.899  85.082  -7.147  1.00  0.00           H
HETATM   47  HHD HEM C1542       7.471  79.584 -10.314  1.00  0.00           H
HETATM   48 HMA1 HEM C1542       5.559  79.043  -0.235  1.00  0.00           H
HETATM   49 HMA2 HEM C1542       7.298  79.091   0.141  1.00  0.00           H
HETATM   50 HMA3 HEM C1542       6.446  80.584  -0.319  1.00  0.00           H
HETATM   51 HAA2 HEM C1542       7.055  75.989  -2.750  1.00  0.00           H
HETATM   52 HAA3 HEM C1542       6.696  76.625  -1.151  1.00  0.00           H
HETATM   53 HBA2 HEM C1542       9.413  76.181  -2.367  1.00  0.00           H
HETATM   54 HBA3 HEM C1542       9.139  76.933  -0.797  1.00  0.00           H
HETATM   55 HMB1 HEM C1542       6.754  84.582  -1.370  1.00  0.00           H
HETATM   56 HMB2 HEM C1542       5.404  85.541  -2.024  1.00  0.00           H
HETATM   57 HMB3 HEM C1542       5.120  83.880  -1.449  1.00  0.00           H
HETATM   58  HAB HEM C1542       6.752  86.720  -5.381  1.00  0.00           H
HETATM   59 HBB1 HEM C1542       4.144  86.288  -3.826  1.00  0.00           H
HETATM   60 HBB2 HEM C1542       4.756  87.869  -4.617  1.00  0.00           H
HETATM   61 HMC1 HEM C1542       6.821  85.505  -9.465  1.00  0.00           H
HETATM   62 HMC2 HEM C1542       6.103  84.743 -10.905  1.00  0.00           H
HETATM   63 HMC3 HEM C1542       5.122  84.976  -9.438  1.00  0.00           H
HETATM   64  HAC HEM C1542       7.107  81.114 -11.904  1.00  0.00           H
HETATM   65 HBC1 HEM C1542       4.997  83.310 -11.823  1.00  0.00           H
HETATM   66 HBC2 HEM C1542       5.520  82.255 -13.277  1.00  0.00           H
HETATM   67 HMD1 HEM C1542       8.317  75.873 -10.060  1.00  0.00           H
HETATM   68 HMD2 HEM C1542       6.923  76.868 -10.545  1.00  0.00           H
HETATM   69 HMD3 HEM C1542       8.575  77.510 -10.710  1.00  0.00           H
HETATM   70 HAD2 HEM C1542       8.542  74.879  -8.229  1.00  0.00           H
HETATM   71 HAD3 HEM C1542       8.796  75.136  -6.477  1.00  0.00           H
HETATM   72 HBD2 HEM C1542       6.074  74.954  -7.879  1.00  0.00           H
HETATM   73 HBD3 HEM C1542       6.316  75.243  -6.143  1.00  0.00           H
END


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