[gmx-users] GPCR insertion in POPC_Cholesterol bilayer

[Justin Lemkul]

On 6/12/17 4:12 PM, Archana Sonawani-Jagtap wrote:
> Hi all,
> 
> I am trying to insert GPCR in POPC with 30 mol% cholesterol bilayer. I am
> using Inflategro method for insertion but I found that the script does not
> recognize the cholesterol molecules and they are not inflated during the
> inflation step.
> 

If you have more than one lipid type, you have to modify the script to account 
for this fact.

> Can anyone please let me know which method to use or I have missed any step?
> 

Build it online with CHARMM-GUI.

-Justin

-- 
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Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

jalemkul at outerbanks.umaryland.edu | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

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