[gmx-users] Topology file of molecule

Krzysztof Kolman krzysztof.kolman at gmail.com
Thu Nov 23 10:24:21 CET 2017


Dear Gromacs Users,

I would like to perform a simulation of adsorption of 3,4-Dihydroxybenzoic
acid (34DHBA) on silica surface using the OPLS-AA forcefield. I created
topology files for the silica surface based on the information found in
papers describing simulations of quartz (Wensink 2000
- 10.1021/la053284f, van der Spoel 2006 -10.1021/la053284f ). The topology
for 34DHBA was created using STaGE script (Lundborg 2015
- 10.1021/jp505332p) but some of the parameters (dihedrals) were missing
and GAFF parameters were used instead. Please find below the content of itp
file:

[ moleculetype ]
; Name            nrexcl
 34DHBA_opls 3

[ atoms ]
;   nr  type  resi  res  atom  cgnr     charge      mass       ; qtot
 bond_type
  1    opls_145 1   LIG   C1    1     -0.115000   12.01100     ; -0.115000
 CA
  2    opls_166 1   LIG   C2    2      0.150000   12.01100     ; 0.035000
 CA
  3    opls_166 1   LIG   C3    3      0.150000   12.01100     ; 0.185000
 CA
  4    opls_145 1   LIG   C4    4     -0.115000   12.01100     ; 0.070000
 CA
  5    opls_145 1   LIG   C5    5     -0.115000   12.01100     ; -0.045000
 CA
  6    opls_145 1   LIG   C6    6     -0.115000   12.01100     ; -0.160000
 CA
  7    opls_167 1   LIG   OC3   7     -0.585000   15.99940     ; -0.745000
 OH
  8    opls_167 1   LIG   OC2   8     -0.585000   15.99940     ; -1.330000
 OH
  9    opls_470 1   LIG   C9    9      0.635000   12.01100     ; -0.695000
 C
  10   opls_269 1   LIG   OC91  10    -0.440000   15.99940     ; -1.135000
 O_3
  11   opls_268 1   LIG   OC92  11    -0.530000   15.99940     ; -1.665000
 OH
  12   opls_146 1   LIG   HC1   12     0.115000    1.00800     ; -1.550000
 HA
  13   opls_146 1   LIG   HC4   13     0.115000    1.00800     ; -1.435000
 HA
  14   opls_146 1   LIG   HC6   14     0.115000    1.00800     ; -1.320000
 HA
  15   opls_168 1   LIG   HO3   15     0.435000    1.00800     ; -0.885000
 HO
  16   opls_168 1   LIG   HO2   16     0.435000    1.00800     ; -0.450000
 HO
  17   opls_270 1   LIG   HO92  17     0.450000    1.00800     ; 0.000000
 HO

[ bonds ]
;   ai     aj funct   r             k
    1   2   1   1.4000e-01   3.9246e+05 ; CA - CA
    1   6   1   1.4000e-01   3.9246e+05 ; CA - CA
    1   12   1   1.0800e-01   3.0711e+05 ; CA - HA
    2   3   1   1.4000e-01   3.9246e+05 ; CA - CA
    2   8   1   1.3640e-01   3.7656e+05 ; CA - OH
    3   4   1   1.4000e-01   3.9246e+05 ; CA - CA
    3   7   1   1.3640e-01   3.7656e+05 ; CA - OH
    4   5   1   1.4000e-01   3.9246e+05 ; CA - CA
    4   13   1   1.0800e-01   3.0711e+05 ; CA - HA
    5   6   1   1.4000e-01   3.9246e+05 ; CA - CA
    5   9   1   1.4900e-01   3.3472e+05 ; CA - C
    6   14   1   1.0800e-01   3.0711e+05 ; CA - HA
    7   15   1   9.4500e-02   4.6275e+05 ; OH - HO
    8   16   1   9.4500e-02   4.6275e+05 ; OH - HO
    9   10   1   1.2290e-01   4.7698e+05 ; C - O_3
    9   11   1   1.3640e-01   3.7656e+05 ; C - OH
    11   17   1   9.4500e-02   4.6275e+05 ; OH - HO

[ pairs ]
;   ai     aj    funct
     1      4      1 ;     C1 - C4
     1      7      1 ;     C1 - OC3
     1      9      1 ;     C1 - C9
     1     16      1 ;     C1 - HO2
     2      5      1 ;     C2 - C5
     2     13      1 ;     C2 - HC4
     2     14      1 ;     C2 - HC6
     2     15      1 ;     C2 - HO3
     3      9      1 ;     C3 - C9
     3     16      1 ;     C3 - HO2
     4      8      1 ;     C4 - OC2
     4     10      1 ;     C4 - OC91
     4     11      1 ;     C4 - OC92
     4     14      1 ;     C4 - HC6
     4     15      1 ;     C4 - HO3
     5      7      1 ;     C5 - OC3
     5     17      1 ;     C5 - HO92
     6      3      1 ;     C6 - C3
     6      8      1 ;     C6 - OC2
     6     10      1 ;     C6 - OC91
     6     11      1 ;     C6 - OC92
     6     13      1 ;     C6 - HC4
     7      8      1 ;    OC3 - OC2
     7     13      1 ;    OC3 - HC4
     9     13      1 ;     C9 - HC4
     9     14      1 ;     C9 - HC6
    10     17      1 ;   OC91 - HO92
    12      3      1 ;    HC1 - C3
    12      5      1 ;    HC1 - C5
    12      8      1 ;    HC1 - OC2
    12     14      1 ;    HC1 - HC6

[ angles ]
;   ai     aj     ak    funct   theta         cth
    1   2   3   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    1   2   8   1   1.2000e+02   5.8576e+02 ; CA - CA - OH
    1   6   5   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    1   6   14   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    2   1   6   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    2   1   12   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    2   3   4   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    2   3   7   1   1.2000e+02   5.8576e+02 ; CA - CA - OH
    2   8   16   1   1.1300e+02   2.9288e+02 ; CA - OH - HO
    3   2   8   1   1.2000e+02   5.8576e+02 ; CA - CA - OH
    3   4   5   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    3   4   13   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    3   7   15   1   1.1300e+02   2.9288e+02 ; CA - OH - HO
    4   3   7   1   1.2000e+02   5.8576e+02 ; CA - CA - OH
    4   5   6   1   1.2000e+02   5.2718e+02 ; CA - CA - CA
    4   5   9   1   1.2000e+02   7.1128e+02 ; CA - CA - C
    5   4   13   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    5   6   14   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    5   9   10   1   1.2040e+02   6.6944e+02 ; CA - C - O_3
    5   9   11   1   1.2000e+02   5.8576e+02 ; CA - C - OH
    6   1   12   1   1.2000e+02   2.9288e+02 ; CA - CA - HA
    6   5   9   1   1.2000e+02   7.1128e+02 ; CA - CA - C
    9   11   17   1   1.1300e+02   2.9288e+02 ; C - OH - HO
    10   9   11   1   1.2100e+02   6.6944e+02 ; O_3 - C - OH

[ dihedrals ] ; propers
; for gromacs 4.5 or higher, using funct 9
;    i      j      k      l   func   phase     kd      pn
     1      2      3      4      9   180.00  15.16700   2 ;     C1-    C2-
  C3-    C4  !Warning using GAFF parameters!
     1      2      3      7      9   180.00  15.16700   2 ;     C1-    C2-
  C3-   OC3  !Warning using GAFF parameters!
     1      2      8     16      9   180.00   3.76560   2 ;     C1-    C2-
 OC2-   HO2  !Warning using GAFF parameters!
     1      6      5      4      9   180.00  15.16700   2 ;     C1-    C6-
  C5-    C4  !Warning using GAFF parameters!
     1      6      5      9      9   180.00  15.16700   2 ;     C1-    C6-
  C5-    C9  !Warning using GAFF parameters!
     2      1      6      5      9   180.00  15.16700   2 ;     C2-    C1-
  C6-    C5  !Warning using GAFF parameters!
     2      1      6     14      9   180.00  15.16700   2 ;     C2-    C1-
  C6-   HC6  !Warning using GAFF parameters!
     2      3      4      5      9   180.00  15.16700   2 ;     C2-    C3-
  C4-    C5  !Warning using GAFF parameters!
     2      3      4     13      9   180.00  15.16700   2 ;     C2-    C3-
  C4-   HC4  !Warning using GAFF parameters!
     2      3      7     15      9   180.00   3.76560   2 ;     C2-    C3-
 OC3-   HO3  !Warning using GAFF parameters!
     3      2      8     16      9   180.00   3.76560   2 ;     C3-    C2-
 OC2-   HO2  !Warning using GAFF parameters!
     3      4      5      6      9   180.00  15.16700   2 ;     C3-    C4-
  C5-    C6  !Warning using GAFF parameters!
     3      4      5      9      9   180.00  15.16700   2 ;     C3-    C4-
  C5-    C9  !Warning using GAFF parameters!
     4      3      2      8      9   180.00  15.16700   2 ;     C4-    C3-
  C2-   OC2  !Warning using GAFF parameters!
     4      3      7     15      9   180.00   3.76560   2 ;     C4-    C3-
 OC3-   HO3  !Warning using GAFF parameters!
     4      5      6     14      9   180.00  15.16700   2 ;     C4-    C5-
  C6-   HC6  !Warning using GAFF parameters!
     4      5      9     10      9   180.00   4.18400   2 ;     C4-    C5-
  C9-  OC91  !Warning using GAFF parameters!
     4      5      9     11      9   180.00   4.18400   2 ;     C4-    C5-
  C9-  OC92  !Warning using GAFF parameters!
     5      4      3      7      9   180.00  15.16700   2 ;     C5-    C4-
  C3-   OC3  !Warning using GAFF parameters!
     5      9     11     17      9   180.00   9.62320   2 ;     C5-    C9-
OC92-  HO92  !Warning using GAFF parameters!
     6      1      2      3      9   180.00  15.16700   2 ;     C6-    C1-
  C2-    C3  !Warning using GAFF parameters!
     6      1      2      8      9   180.00  15.16700   2 ;     C6-    C1-
  C2-   OC2  !Warning using GAFF parameters!
     6      5      4     13      9   180.00  15.16700   2 ;     C6-    C5-
  C4-   HC4  !Warning using GAFF parameters!
     6      5      9     10      9   180.00   4.18400   2 ;     C6-    C5-
  C9-  OC91  !Warning using GAFF parameters!
     6      5      9     11      9   180.00   4.18400   2 ;     C6-    C5-
  C9-  OC92  !Warning using GAFF parameters!
     7      3      2      8      9   180.00  15.16700   2 ;    OC3-    C3-
  C2-   OC2  !Warning using GAFF parameters!
     7      3      4     13      9   180.00  15.16700   2 ;    OC3-    C3-
  C4-   HC4  !Warning using GAFF parameters!
     9      5      4     13      9   180.00  15.16700   2 ;     C9-    C5-
  C4-   HC4  !Warning using GAFF parameters!
     9      5      6     14      9   180.00  15.16700   2 ;     C9-    C5-
  C6-   HC6  !Warning using GAFF parameters!
    10      9     11     17      9     0.00   7.94960   1 ;   OC91-    C9-
OC92-  HO92  !Warning using GAFF parameters!
    10      9     11     17      9   180.00   9.62320   2 ;   OC91-    C9-
OC92-  HO92  !Warning using GAFF parameters!
    12      1      2      3      9   180.00  15.16700   2 ;    HC1-    C1-
  C2-    C3  !Warning using GAFF parameters!
    12      1      2      8      9   180.00  15.16700   2 ;    HC1-    C1-
  C2-   OC2  !Warning using GAFF parameters!
    12      1      6      5      9   180.00  15.16700   2 ;    HC1-    C1-
  C6-    C5  !Warning using GAFF parameters!
    12      1      6     14      9   180.00  15.16700   2 ;    HC1-    C1-
  C6-   HC6  !Warning using GAFF parameters!

[ dihedrals ] ; impropers
; treated as propers in GROMACS to use correct AMBER analytical function
;    i      j      k      l   func   phase     kd      pn
     1      3      2      8      4   180.00   4.60240   2 ;     C1-    C3-
  C2-   OC2  !Warning using GAFF parameters!
     1      5      6     14      4   180.00   4.60240   2 ;     C1-    C5-
  C6-   HC6  !Warning using GAFF parameters!
     2      4      3      7      4   180.00   4.60240   2 ;     C2-    C4-
  C3-   OC3  !Warning using GAFF parameters!
     3      5      4     13      4   180.00   4.60240   2 ;     C3-    C5-
  C4-   HC4  !Warning using GAFF parameters!
     5     10      9     11      4   180.00  43.93200   2 ;     C5-  OC91-
  C9-  OC92  !Warning using GAFF parameters!
     9      4      5      6      4   180.00   4.60240   2 ;     C9-    C4-
  C5-    C6  !Warning using GAFF parameters!
    12      1      6      2      4   180.00   4.60240   2 ;    HC1-    C1-
  C6-    C2  !Warning using GAFF parameters!

Generally one should not mix different forcefiedls but my question is how
big "mistake" I make running a simulation using the parameters shown above.
Is there any benchmark that allows to evaluate the quality of a topology
file (maybe by calculating some experimental properties)?

Thank you in advance for your help.

Best regards,
Krzysztof


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