[gmx-users] umbrella sampling

rose rahmani rose.rhmn93 at gmail.com
Fri Feb 2 14:44:00 CET 2018


 Hi, i'm doing umbrella sampling.(pulling AA toward nanosheet).and 4nS
simulation for each window.

>> g_energy -f umbrella0.edr -o out.xvg
 1  Bond             2  Angle            3  Proper-Dih.      4
Improper-Dih.
  5  LJ-14            6  Coulomb-14       7  LJ-(SR)          8
Coulomb-(SR)
  9  Coul.-recip.    10  COM-Pull-En.    11  Potential       12
Kinetic-En.
 13  Total-Energy    14  Conserved-En.   15  Temperature     16  Pressure

 17  Constr.-rmsd    18  Vir-XX          19  Vir-XY          20  Vir-XZ

 21  Vir-YX          22  Vir-YY          23  Vir-YZ          24  Vir-ZX

 25  Vir-ZY          26  Vir-ZZ          27  Pres-XX         28  Pres-XY

 29  Pres-XZ         30  Pres-YX         31  Pres-YY         32  Pres-YZ

 33  Pres-ZX         34  Pres-ZY         35  Pres-ZZ         36
#Surf*SurfTen
 37  Mu-X                                38  Mu-Y

 39  Mu-Z                                40  Coul-SR:SOL-SOL

 41  LJ-SR:SOL-SOL                       42  Coul-14:SOL-SOL

 43  LJ-14:SOL-SOL                       44  Coul-SR:SOL-WAL

 45  LJ-SR:SOL-WAL                       46  Coul-14:SOL-WAL

 47  LJ-14:SOL-WAL                       48  Coul-SR:SOL-ZnS

 49  LJ-SR:SOL-ZnS                       50  Coul-14:SOL-ZnS

 51  LJ-14:SOL-ZnS                       52  Coul-SR:SOL-Protein

 53  LJ-SR:SOL-Protein                   54  Coul-14:SOL-Protein

 55  LJ-14:SOL-Protein                   56  Coul-SR:SOL-NA

 57  LJ-SR:SOL-NA    58  Coul-14:SOL-NA  59  LJ-14:SOL-NA    60
Coul-SR:SOL-CL
 61  LJ-SR:SOL-CL                        62  Coul-14:SOL-CL

 63  LJ-14:SOL-CL                        64  Coul-SR:SOL-wall0

 65  LJ-SR:SOL-wall0                     66  Coul-14:SOL-wall0

 67  LJ-14:SOL-wall0                     68  Coul-SR:SOL-wall1

 69  LJ-SR:SOL-wall1                     70  Coul-14:SOL-wall1

 71  LJ-14:SOL-wall1                     72  Coul-SR:WAL-WAL

 73  LJ-SR:WAL-WAL                       74  Coul-14:WAL-WAL

 75  LJ-14:WAL-WAL                       76  Coul-SR:WAL-ZnS

 77  LJ-SR:WAL-ZnS                       78  Coul-14:WAL-ZnS

 79  LJ-14:WAL-ZnS                       80  Coul-SR:WAL-Protein

 81  LJ-SR:WAL-Protein                   82  Coul-14:WAL-Protein

 83  LJ-14:WAL-Protein                   84  Coul-SR:WAL-NA

 85  LJ-SR:WAL-NA    86  Coul-14:WAL-NA  87  LJ-14:WAL-NA    88
Coul-SR:WAL-CL
 89  LJ-SR:WAL-CL                        90  Coul-14:WAL-CL

 91  LJ-14:WAL-CL                        92  Coul-SR:WAL-wall0

 93  LJ-SR:WAL-wall0                     94  Coul-14:WAL-wall0

 95  LJ-14:WAL-wall0                     96  Coul-SR:WAL-wall1

 97  LJ-SR:WAL-wall1                     98  Coul-14:WAL-wall1

 99  LJ-14:WAL-wall1                    100  Coul-SR:ZnS-ZnS

101  LJ-SR:ZnS-ZnS                      102  Coul-14:ZnS-ZnS

103  LJ-14:ZnS-ZnS                      104  Coul-SR:ZnS-Protein

105  LJ-SR:ZnS-Protein                  106  Coul-14:ZnS-Protein

107  LJ-14:ZnS-Protein                  108  Coul-SR:ZnS-NA

109  LJ-SR:ZnS-NA   110  Coul-14:ZnS-NA 111  LJ-14:ZnS-NA   112
Coul-SR:ZnS-CL
113  LJ-SR:ZnS-CL                       114  Coul-14:ZnS-CL

115  LJ-14:ZnS-CL                       116  Coul-SR:ZnS-wall0

117  LJ-SR:ZnS-wall0                    118  Coul-14:ZnS-wall0

119  LJ-14:ZnS-wall0                    120  Coul-SR:ZnS-wall1

121  LJ-SR:ZnS-wall1                    122  Coul-14:ZnS-wall1

123  LJ-14:ZnS-wall1                    124  Coul-SR:Protein-Protein

125  LJ-SR:Protein-Protein              126  Coul-14:Protein-Protein

127  LJ-14:Protein-Protein              128  Coul-SR:Protein-NA

129  LJ-SR:Protein-NA                   130  Coul-14:Protein-NA

131  LJ-14:Protein-NA                   132  Coul-SR:Protein-CL

133  LJ-SR:Protein-CL                   134  Coul-14:Protein-CL

135  LJ-14:Protein-CL                   136  Coul-SR:Protein-wall0

137  LJ-SR:Protein-wall0                138  Coul-14:Protein-wall0

139  LJ-14:Protein-wall0                140  Coul-SR:Protein-wall1

141  LJ-SR:Protein-wall1                142  Coul-14:Protein-wall1

143  LJ-14:Protein-wall1                144  Coul-SR:NA-NA

145  LJ-SR:NA-NA    146  Coul-14:NA-NA  147  LJ-14:NA-NA    148
Coul-SR:NA-CL
149  LJ-SR:NA-CL                        150  Coul-14:NA-CL

151  LJ-14:NA-CL                        152  Coul-SR:NA-wall0

153  LJ-SR:NA-wall0                     154  Coul-14:NA-wall0

155  LJ-14:NA-wall0                     156  Coul-SR:NA-wall1

157  LJ-SR:NA-wall1                     158  Coul-14:NA-wall1

159  LJ-14:NA-wall1 160  Coul-SR:CL-CL  161  LJ-SR:CL-CL    162
Coul-14:CL-CL
163  LJ-14:CL-CL                        164  Coul-SR:CL-wall0

165  LJ-SR:CL-wall0                     166  Coul-14:CL-wall0

167  LJ-14:CL-wall0                     168  Coul-SR:CL-wall1

169  LJ-SR:CL-wall1                     170  Coul-14:CL-wall1

171  LJ-14:CL-wall1                     172  Coul-SR:wall0-wall0

173  LJ-SR:wall0-wall0                  174  Coul-14:wall0-wall0

175  LJ-14:wall0-wall0                  176  Coul-SR:wall0-wall1

177  LJ-SR:wall0-wall1                  178  Coul-14:wall0-wall1

179  LJ-14:wall0-wall1                  180  Coul-SR:wall1-wall1

181  LJ-SR:wall1-wall1                  182  Coul-14:wall1-wall1

183  LJ-14:wall1-wall1                  184  T-System

185  Lamb-System

how can i understand that this 4nS simulation is enough for all 30 windows
which i selected after pulling? as you see i'm doing NVT.i tried to
calculate temperature(15) and total energy(13) and potential(11) for
example for first window;
Energy                      Average   Err.Est.       RMSD  Tot-Drift
------------------------------------------------------------
-------------------
Potential                    -72708        9.2    227.038   -38.8081
(kJ/mol)
Total Energy               -63763.9        8.7    273.747   -34.9771
(kJ/mol)
Temperature                 299.977      0.022    5.04346   0.128492  (K)
plots show that temperature is ok and total energy doesn't have
considerable fluctuation (except after first moment). but potential
fluctuates. since i don't see sharp peaks in PMF (but good trends), is it
rational to do long time simulation? what is the main clue for knowing
that? is it potential?
and what exactly total energy(13)  refers to in my system?
would you please help me?

Thank you for your all considerations


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