[gmx-users] Fw: GDP topology

Michal Kolinski mkolin at iimcb.gov.pl
Mon Dec 13 13:04:39 CET 2004



Hi all!

I need to simulate rhodopsin in lipid bilayer with G protein + GDP

I'm not certain if the topology file  (charges)  for GDP I have obtained using PRODRG

server is right for me.  I plan to use (ffgmx_lipids.tar.gz)  ffgmx force field modified 

to use with lipids from gromacs site.  (At the beginning I did a short simulation of  GDP 

in box of water only and everything seemed fine).  Please give me some comment and

thank you in advice. 



here is part of my gdp.itp file:

[ moleculetype ]
; Name nrexcl
GDP      3

[ atoms ]
;   nr      type  resnr resid  atom  cgnr   charge     mass
     1        OA     1  GDP     O2B     1   -1.144  15.9994
     2         P     1  GDP      PB     1    1.123  30.9738
     3        OA     1  GDP     O3B     1   -1.145  15.9994
     4        OM     1  GDP     O1B     1   -0.834  15.9994
     5        OS     1  GDP     O3A     2   -0.176  15.9994
     6         P     1  GDP      PA     2    1.404  30.9738
     7        OM     1  GDP     O1A     2   -0.526  15.9994
     8        OM     1  GDP     O2A     2   -0.526  15.9994
     9        OS     1  GDP     O5*     2   -0.176  15.9994
    10       CH2     1  GDP     C5*     3    0.034  14.0270
    11       CS1     1  GDP     C4*     3    0.180  13.0190
    12        OS     1  GDP     O4*     3   -0.214  15.9994
    13       CH1     1  GDP     C3*     4    0.133  13.0190
    14        OA     1  GDP     O3*     4   -0.160  15.9994
    15        HO     1  GDP     HAA     4    0.027   1.0080
    16       CH1     1  GDP     C2*     5    0.083  13.0190
    17        OA     1  GDP     O2*     5   -0.256  15.9994
    18        HO     1  GDP     HAB     5    0.016   1.0080
    19       CS1     1  GDP     C1*     5    0.157  13.0190
    20      NR5*     1  GDP      N9     6    0.119  14.0067
    21      CR51     1  GDP      C8     6   -0.119  13.0190
    22       NR5     1  GDP      N7     7   -0.427  14.0067
    23        CB     1  GDP      C5     7    0.132  12.0110
    24        CB     1  GDP      C4     7    0.302  12.0110
    25       NR6     1  GDP      N3     7   -0.438  14.0067
    26        CB     1  GDP      C2     7    0.431  12.0110
    27        NT     1  GDP      N2     8    0.039  14.0067
    28         H     1  GDP     HAE     8   -0.019   1.0080
    29         H     1  GDP     HAD     8   -0.020   1.0080
    30      NR6*     1  GDP      N1     9    0.005  14.0067
    31         H     1  GDP     HAC     9   -0.038   1.0080
    32        CB     1  GDP      C6     9    0.157  12.0110
    33         O     1  GDP      O6     9   -1.124  15.9994

[ bonds ]
; ai  aj  fu    c0, c1, ...
   1   2   1    0.161    251040.0    0.161    251040.0 ;   O2B   PB
   2   3   1    0.161    251040.0    0.161    251040.0 ;    PB  O3B
   2   4   1    0.148    376560.0    0.148    376560.0 ;    PB  O1B
   2   5   1    0.161    251040.0    0.161    251040.0 ;    PB  O3A
   5   6   1    0.161    251040.0    0.161    251040.0 ;   O3A   PA
   6   7   1    0.148    376560.0    0.148    376560.0 ;    PA  O1A
   6   8   1    0.148    376560.0    0.148    376560.0 ;    PA  O2A
   6   9   1    0.161    251040.0    0.161    251040.0 ;    PA  O5*
   9  10   1    0.143    251040.0    0.143    251040.0 ;   O5*  C5*
  10  11   1    0.153    251040.0    0.153    251040.0 ;   C5*  C4*
  11  12   1    0.144    251040.0    0.144    251040.0 ;   C4*  O4*
  11  13   1    0.153    334720.0    0.153    334720.0 ;   C4*  C3*
  12  19   1    0.144    251040.0    0.144    251040.0 ;   O4*  C1*
  13  14   1    0.143    334720.0    0.143    334720.0 ;   C3*  O3*
  13  16   1    0.153    334720.0    0.153    334720.0 ;   C3*  C2*
  14  15   1    0.100    313800.0    0.100    313800.0 ;   O3*  HAA
  16  17   1    0.143    334720.0    0.143    334720.0 ;   C2*  O2*
  16  19   1    0.153    334720.0    0.153    334720.0 ;   C2*  C1*
  17  18   1    0.100    313800.0    0.100    313800.0 ;   O2*  HAB
  19  20   1    0.148    251040.0    0.148    251040.0 ;   C1*   N9
  20  21   1    0.133    418400.0    0.133    418400.0 ;    N9   C8
  20  24   1    0.133    418400.0    0.133    418400.0 ;    N9   C4
  21  22   1    0.133    418400.0    0.133    418400.0 ;    C8   N7
  22  23   1    0.133    418400.0    0.133    418400.0 ;    N7   C5
  23  24   1    0.139    418400.0    0.139    418400.0 ;    C5   C4
  23  32   1    0.139    418400.0    0.139    418400.0 ;    C5   C6
  24  25   1    0.134    418400.0    0.134    418400.0 ;    C4   N3
  25  26   1    0.134    418400.0    0.134    418400.0 ;    N3   C2
  26  27   1    0.133    376560.0    0.133    376560.0 ;    C2   N2
  26  30   1    0.140    334720.0    0.140    334720.0 ;    C2   N1
  27  28   1    0.100    374468.0    0.100    374468.0 ;    N2  HAE
  27  29   1    0.100    374468.0    0.100    374468.0 ;    N2  HAD
  30  31   1    0.100    374468.0    0.100    374468.0 ;    N1  HAC
  30  32   1    0.140    334720.0    0.140    334720.0 ;    N1   C6
  32  33   1    0.123    502080.0    0.123    502080.0 ;    C6   O6

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