[gmx-users] Problems using grompp and amber force field

root mario at exactas.unlpam.edu.ar
Tue May 8 02:27:03 CEST 2007


Dear GROMACS user:

Erik and Tom thanks by the comments, too. Sorry, I read the FAQ pages of
http://folding.stanford.edu and now I understand your comments.  
We tried with cpp = /lib/cpp -traditional in the mdp file. The warning
desapears, but the error messages is similar.
(Fatal error:
> >> Atoms in the .top are not numbered consecutively from 1
> >>
> >> -------------------------------------------------------
> >>
> >> We tested the dick.top file, and we verified that the atoms
> >         are numbered
> >> consecutively from 1 to 758.)

We download the ffamber_v3.3.1-doc.tar.gz, that we considered that
correspond to the 3.3.1 version of GROMACS.
Is this correct?

Thanks in advance.

Mario
 
El lun, 07-05-2007 a las 23:17 +0200, Erik Marklund escribió:
> As someone already mentioned, the following can be found among the  
> AMBERport FAQ:
> 
>  >>
> Why do I get a grompp warning "missing white space after `#define  
> proper_*'?"
> Set "cpp = /lib/cpp -traditional" in your .mdp file to remove these  
> warnings, which result from the use of the N* atom type of the AMBER  
> family of force fields and can be ignored.
> <<
> 
> Did you try doing that?
> 
> Good luck!
> 
> /Erik
> 
> 7 maj 2007 kl. 22.29 skrev root:
> 
> Hi,

>I think if you set "cpp = /lib/cpp -traditional" in your .mdp file then
>it 
>should get rid of the warnings about "spaces are absent in target after
>the 
>name of macro". For more info see 
><http://folding.stanford.edu/ffamber/FAQ.html>

>Tom

>--On 07 May 2007 20:42 +0200 David van der Spoel
><spoel at xray.bmc.uu.se> 
>wrote:
> > David and GROMACS users:
> > Sorry we changed the error message in ...
> >
> > /usr/local/gromacs/share/top/ffamber03bon.itp:541:22:  warning: spaces
> > are absent in target after the name of macro
> >
> > because of the original message was in spanish.
> >
> > We checked these files above. We run GROMACS in fedora 6.0
> > We analyzed the warning messages. They correspond to a format of
> > description of a dihedral angle. For example the 538:22 warning
> >  correspond to le line 538 column 22
> >
> >  #define proper_X_CT_N*_X
> >  and there are a format problem with the * symbol.
> >
> >  we don't know that to do still with this.
> >
> >
> > Thanks in advance
> >
> > root wrote:
> >                                 De:
> > David van der Spoel
> > <spoel at xray.bmc.uu.se>
> >                        Responder a:
> > Discussion list for GROMACS users
> > <gmx-users at gromacs.org>
> >                               Para:
> > Discussion list for GROMACS users
> > <gmx-users at gromacs.org>
> >                             Asunto:
> > Re: [gmx-users] Problems using
> > grompp and amber force field
> >                              Fecha:
> > Mon, 07 May 2007 20:42:51 +0200
> > (15:42 ART)
> >
> > The procedure to simulate the DNA dodecamer was:
> >
> > 1) We generate the DNA topology and coordinates in gromacs format  
> > (with
> > the amber03 force field ) using the pdb2gmx program:
> >
> > pdb2gmx -f dickerson.pdb -o dick.gro -p dick.top -water spce
> >
> > 2) and we obtain the box with the dodecamer plus water coordinates
> > using editconf and genbox:
> >
> > editconf -f dick.gro -o dick03.pdb -bf triclinic -box 6 5 6 -angle  
> > 90 90
> > 90
> > genbox -cp dick03.pdb -cs -o dick02.pdb -p dick.top
> >
> > 3) with the files dick03.pdb and dick.top we run grompp:
> >
> > grompp -f em -c dick02.pdb -p dick.top -o dick.tpr -pp topo2.top
> >
> > The output of the program indicate
> > ...
> > creating statusfile for 1 node...
> >
> > Back Off! I just backed up mdout.mdp to ./#mdout.mdp.20#
> > checking input for internal consistency...
> > calling /usr/bin/cpp...
> > In the file included of /usr/local/gromacs/share/top/ffamber03.itp:19,
> >                  de dick.top:11:
> > /usr/local/gromacs/share/top/ffamber03bon.itp:538:22:
> > warning: spaces are absent in target after the name of macro
> > /usr/local/gromacs/share/top/ffamber03bon.itp:541:22:
> > warning: spaces are absent in target after the name of macro
> > /usr/local/gromacs/share/top/ffamber03bon.itp:543:22:
> > warning: spaces are absent in target after the name of macro
> > /usr/local/gromacs/share/top/ffamber03bon.itp:547:21:
> > warning: spaces are absent in target after the name of macro
> > /usr/local/gromacs/share/top/ffamber03bon.itp:559:19:
> > warning: spaces are absent in target after the name of macro
> > processing topology...
> >
> >         check the files above. is the error message correct? then fix
> >         it.
> >
> >         what kind of system are you running this on?
> >
> >> processing topology...
> >> Generated 2701 of the 2701 non-bonded parameter combinations
> >> Generating 1-4 interactions: fudge = 0.5
> >> Generated 2701 of the 2701 1-4 parameter combinations
> >> -------------------------------------------------------
> >> Program grompp, VERSION 3.3.1
> >> Source code file: toppush.c, line: 758
> >>
> >> Fatal error:
> >> Atoms in the .top are not numbered consecutively from 1
> >>
> >> -------------------------------------------------------
> >>
> >> We tested the dick.top file, and we verified that the atoms
> >         are numbered
> >> consecutively from 1 to 758.
> >> We analyzed the warning messages. They correspond to a format
> >         of
> >> desciption of a dihedral angle. For example the 538:22 warning
> >> correspond to le line 538 column 22
> >>
> >> #define proper_X_CT_N*_X
> >> and there are a format problem with the * simbol.
> >>
> >> we don't know that to do still with this.
> >>
> >>
> >>
> >> Thanks in advance
> >>
> >> Mario Campo and Griselda Corral
> >> Fac. de Cs. Exactas y Naturales
> >> UNLPam
> >>
> >>
> >>
> >>
> >> _______________________________________________
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> >
> >
> >         --
> >         David.
> >          
> > ______________________________________________________________________ 
> > __
> >         David van der Spoel, PhD, Assoc. Prof., Molecular Biophysics
> >         group,
> >         Dept. of Cell and Molecular Biology, Uppsala University.
> >         Husargatan 3, Box 596,          75124 Uppsala, Sweden
> >         phone:  46 18 471 4205          fax: 46 18 511 755
> >         spoel at xray.bmc.uu.se    spoel at gromacs.org
> >         http://folding.bmc.uu.se
> >         ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 
> > ++++++++++++
> >         _______________________________________________
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> >
> >
> >
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> 
> _______________________________________________
> Erik Marklund, PhD student
> Laboratory of Molecular Biophysics,
> Dept. of Cell and Molecular Biology, Uppsala University.
> Husargatan 3, Box 596,	75124 Uppsala, Sweden
> phone:	+46 18 471 4537		fax: +46 18 511 755
> erikm at xray.bmc.uu.se	http://xray.bmc.uu.se/molbiophys
> 
> 
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