[gmx-users] problem with genion (gromacs 3.3.99)

Julie Bernauer bernauer at stanford.edu
Wed May 21 23:37:36 CEST 2008


Hi all,

I do have a weird behaviour with genion, which fails with the message:
   ftp2fn: No filetype .???
   No option -cpo
   Segmentation fault

and I can't figure out what I do wrong. I couldn't not find any
reference to it either.
It seems that a file cannot be accessed but I couldn't get in what way. 
As it works fine with gromacs 3.3, I don't really get what the problem 
could be.

The details are below and I can provide other information / files if needed.

Thanks in advance for your help,

-- 
Julie

---------------

Here is what the problem look like:

$ my_genion -s 1bd8_em.tpr -o 1bd8_em.pdb -pname Na -np 4 -g genion.log
                          :-)  G  R  O  M  A  C  S  (-:

                               S  C  A  M  O  R  G

                  :-)  VERSION 3.3.99_development_200800503  (-:


       Written by David van der Spoel, Erik Lindahl, Berk Hess, and others.
        Copyright (c) 1991-2000, University of Groningen, The Netherlands.
              Copyright (c) 2001-2008, The GROMACS development team,
             check out http://www.gromacs.org for more information.

          This program is free software; you can redistribute it and/or
           modify it under the terms of the GNU General Public License
          as published by the Free Software Foundation; either version 2
              of the License, or (at your option) any later version.

                               :-)  my_genion  (-:

Option     Filename  Type         Description
------------------------------------------------------------
   -s    1bd8_em.tpr  Input        Generic run input: tpr tpb tpa xml
-table    table.xvg  Input, Opt.  xvgr/xmgr file
   -n      index.ndx  Input, Opt.  Index file
   -o    1bd8_em.pdb  Output       Generic structure: gro g96 pdb xml
   -g     genion.log  Output       Log file
-pot        pot.pdb  Output, Opt. Protein data bank file
   -p      topol.top  In/Out, Opt. Topology file

Option       Type   Value   Description
------------------------------------------------------
-[no]h       bool   no      Print help info and quit
-nice        int    19      Set the nicelevel
-[no]xvgr    bool   yes     Add specific codes (legends etc.) in the output
                             xvg files for the xmgrace program
-np          int    4       Number of positive ions
-pname       string Na      Name of the positive ion
-pq          int    1       Charge of the positive ion
-nn          int    0       Number of negative ions
-nname       string Cl      Name of the negative ion
-nq          int    -1      Charge of the negative ion
-rmin        real   0.6     Minimum distance between ions
-[no]random  bool   yes     Use random placement of ions instead of based on
                             potential. The rmin option should still work
-seed        int    1993    Seed for random number generator
-scale       real   0.001   Scaling factor for the potential for -pot
-conc        real   0       Specify salt concentration (mol/liter). This
will
                             add sufficient ions to reach up to the
specified
                             concentration as computed from the volume
of the
                             cell in the input tpr file. Overrides the
-np and
                             nn options.
-[no]neutral bool   no      This option will add enough ions to neutralize
                             the system. In combination with the
concentration
                             option a neutral system at a given salt
                             concentration will be generated.


Back Off! I just backed up genion.log to ./#genion.log.8#
WARNING: turning of free energy, will use lambda=0
Reading file 1bd8_em.tpr, VERSION 3.3.99_development_200800503 (single
precision)
ftp2fn: No filetype .???
ftp2fn: No filetype .???
No option -cpo
Segmentation fault

------------

It seems to work well with the standard gromacs 3.3 though (same files, 
only grompp redone):

genion -s 1bd8_em.tpr -o 1bd8_em.pdb -pname Na -np 4 -g genion.log
                          :-)  G  R  O  M  A  C  S  (-:

                      Gyas ROwers Mature At Cryogenic Speed

                             :-)  VERSION 3.3.3  (-:


       Written by David van der Spoel, Erik Lindahl, Berk Hess, and others.
        Copyright (c) 1991-2000, University of Groningen, The Netherlands.
              Copyright (c) 2001-2008, The GROMACS development team,
             check out http://www.gromacs.org for more information.

          This program is free software; you can redistribute it and/or
           modify it under the terms of the GNU General Public License
          as published by the Free Software Foundation; either version 2
              of the License, or (at your option) any later version.

                                 :-)  genion  (-:

Option     Filename  Type         Description
------------------------------------------------------------
   -s    1bd8_em.tpr  Input        Generic run input: tpr tpb tpa xml
-table    table.xvg  Input, Opt.  xvgr/xmgr file
   -n      index.ndx  Input, Opt.  Index file
   -o    1bd8_em.pdb  Output       Generic structure: gro g96 pdb xml
   -g     genion.log  Output       Log file
-pot        pot.pdb  Output, Opt. Protein data bank file
   -p      topol.top  In/Out, Opt. Topology file

Option       Type   Value   Description
------------------------------------------------------
-[no]h       bool   no      Print help info and quit
-[no]X       bool   no      Use dialog box GUI to edit command line options
-nice        int    19      Set the nicelevel
-[no]xvgr    bool   yes     Add specific codes (legends etc.) in the output
                             xvg files for the xmgrace program
-np          int    4       Number of positive ions
-pname       string Na      Name of the positive ion
-pq          int    1       Charge of the positive ion
-nn          int    0       Number of negative ions
-nname       string Cl      Name of the negative ion
-nq          int    -1      Charge of the negative ion
-rmin        real   0.6     Minimum distance between ions
-[no]random  bool   yes     Use random placement of ions instead of based on
                             potential. The rmin option should still work
-seed        int    1993    Seed for random number generator
-scale       real   0.001   Scaling factor for the potential for -pot
-conc        real   0       Specify salt concentration (mol/liter). This
will
                             add sufficient ions to reach up to the
specified
                             concentration as computed from the volume
of the
                             cell in the input tpr file. Overrides the
-np and
                             nn options.
-[no]neutral bool   no      This option will add enough ions to neutralize
                             the system. In combination with the
concentration
                             option a neutral system at a given salt
                             concentration will be generated.


Back Off! I just backed up genion.log to ./#genion.log.1#
WARNING: turning of free energy, will use lambda=0
Reading file 1bd8_em.tpr, VERSION 3.3.3 (single precision)
Using a coulomb cut-off of 1 nm
Will try to add 4 Na ions and 0 Cl ions.
Select a continuous group of solvent molecules
Opening library file /usr/share/gromacs/top/aminoacids.dat
Group     0 (      System) has 36644 elements
Group     1 (     Protein) has  2342 elements
Group     2 (   Protein-H) has  1168 elements
Group     3 (     C-alpha) has   156 elements
Group     4 (    Backbone) has   468 elements
Group     5 (   MainChain) has   625 elements
Group     6 (MainChain+Cb) has   766 elements
Group     7 ( MainChain+H) has   777 elements
Group     8 (   SideChain) has  1565 elements
Group     9 ( SideChain-H) has   543 elements
Group    10 ( Prot-Masses) has  2342 elements
Group    11 ( Non-Protein) has 34302 elements
Group    12 (         SOL) has 34302 elements
Group    13 (       Other) has 34302 elements
Select a group: 12
Selected 12: 'SOL'
Number of (3-atomic) solvent molecules: 11434
Replacing solvent molecule 1341 (atom 6365) with Na
Replacing solvent molecule 8663 (atom 28331) with Na
Replacing solvent molecule 5581 (atom 19085) with Na
Replacing solvent molecule 9673 (atom 31361) with Na


Back Off! I just backed up 1bd8_em.pdb to ./#1bd8_em.pdb.1#

gcq#122: "The Path Of the Righteous Man is Beset On All Sides With the
Iniquities Of the Selfish and the Tyranny Of Evil Men." (Pulp Fiction)




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