[gmx-users] Running MD only for selected part of molecule

vivek sharma viveksharma.iitb at gmail.com
Fri Oct 17 08:02:59 CEST 2008


Hi Justin,
Thanks for your reply...
and apologies for asking you again.

If I want to keep a part of my molecule fix, say part out of 12 angstrom
radius from the ligand and also want to make avoid energy calculation for
the same, (using energy_excl)
Do I need to mention the separate group for that part of molecule, (as I
already did to make that part position restrained), and next how I will
mention those part in .mdp file...
Is it like...
energy_excl           groupname protein/solvent   (if I want to avoid energy
calculation between the group and protein/solvent)
or something else need to be done than that (like during genpr option).

Please explain....As I have no idea to play with such option...

My apologies again, if these small question irritates you.......

With Thanks,
Vivek

2008/10/15 Justin A. Lemkul <jalemkul at vt.edu>

>
>
> vivek sharma wrote:
>
>>
>> Dear Justin,
>> Thanks for your reply..
>> But I am not getting how and where should I give the option of
>> energy_excl. For freezing a part of molecule ?
>>
>
> In the .mdp file.
>
>  what are the other checks to be done for the same ?
>>
>>
> I don't know what you're asking.
>
> -Justin
>
>  With Thanks,
>> Vivek
>> 2008/10/14 Justin A. Lemkul <jalemkul at vt.edu <mailto:jalemkul at vt.edu>>
>>
>>
>>
>>    vivek sharma wrote:
>>
>>
>>
>>        2008/10/14 Justin A. Lemkul <jalemkul at vt.edu
>>        <mailto:jalemkul at vt.edu> <mailto:jalemkul at vt.edu
>>
>>        <mailto:jalemkul at vt.edu>>>
>>
>>
>>
>>
>>           vivek sharma wrote:
>>
>>               Hi there,
>>
>>               I tried the mdrun by keeping a group of residue position
>>               restrained, and it is working well (verified by comparing
>>        RMSD
>>               plot for the same), thanks for your suggestions regarding
>>        the same.
>>               But, I have seen that the part of the molecule I kept for
>>        PR is
>>               still having some motion, how can I keep that part rigid ?
>>
>>
>>           Position restraints do not necessarily guarantee that atomic
>>           positions stay absolutely fixed.  Instead, there is an energy
>>           penalty for moving them, which could in some cases be overcome.
>>
>>
>>               I am not getting any idea of energy_excl option mentioned
>>        above,
>>               please explain.
>>
>>
>>           The freezegrps option can be used to fix atomic positions, but
>> in
>>           this case large forces can be generated within the frozen
>>        group.  As
>>           such, you can apply energygrp_excl to exclude energetic terms
>>        within
>>           the frozen group.
>>
>>
>>        Will it help me in reducing the time taken for the simulation if
>>        I'll keep the part of molecule frozen ?
>>
>>
>>    I've never used freezegrps for any large set of atoms, so maybe.  If
>>    you've got surrounding solvent, though, the nonbonded interactions
>>    are still calculated between the frozen group and solvent, unless
>>    you turn that off too.  But then it starts getting really
>>    unphysical, in my view.
>>
>>    -Justin
>>
>>        Thanks,
>>        ~Vivek
>>
>>
>>
>>           -Justin
>>
>>               With Thanks,
>>               Vivek
>>
>>               2008/10/12 Erik Lindahl <lindahl at cbr.su.se
>>        <mailto:lindahl at cbr.su.se>
>>               <mailto:lindahl at cbr.su.se <mailto:lindahl at cbr.su.se>>
>>        <mailto:lindahl at cbr.su.se <mailto:lindahl at cbr.su.se>
>>
>>               <mailto:lindahl at cbr.su.se <mailto:lindahl at cbr.su.se>>>>
>>
>>
>>
>>                  Hi,
>>
>>
>>                  On Oct 10, 2008, at 12:46 PM, Justin A. Lemkul wrote:
>>
>>
>>                          I want to run MD over a part of my molecule ,
>>        for few
>>                          residues only (not the whole molecule).
>>                          Can I do it using GROMACS ?
>>                          I searched for the online documentation and
>>        mailing list,
>>                          but unable to get appropriate information.
>>                          If somebody has already tried such things
>>        earlier, please
>>                          suggest and direct me for appropriate link and
>>        address.
>>
>>
>>                      Well, if your goal is to keep certain parts fixed
>>        and allow
>>                      others to move, probably the easiest way to do it
>>        is to apply
>>                      position restraints to the "fixed" part.
>>
>>
>>                  You can also set parts of the system as a freeze
>>        group, in which
>>                  case you can exclude all nonbonded interactions inside
>>        the freeze
>>                  group with the energygrp_excl option in your mdp file.
>>
>>                  The main advantage of this is of course that you will
>>        improve
>>                  performance if 99% of your system is frozen (although all
>>                  interactions between the frozen and non-frozen parts
>> still
>>               have to
>>                  be calculated). On the other hand, completely freezing
>>        part
>>               of the
>>                  system is not very realistic, and you're likely to get
>>        strange
>>                  behavior in the interface...
>>
>>                  Cheers,
>>
>>                  Erik
>>
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>>           --    ========================================
>>
>>           Justin A. Lemkul
>>           Graduate Research Assistant
>>           Department of Biochemistry
>>           Virginia Tech
>>           Blacksburg, VA
>>           jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu> | (540)
>>        231-9080
>>
>>           http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>>           ========================================
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>>
>>    --    ========================================
>>
>>    Justin A. Lemkul
>>    Graduate Research Assistant
>>    Department of Biochemistry
>>    Virginia Tech
>>    Blacksburg, VA
>>    jalemkul[at]vt.edu <http://vt.edu> | (540) 231-9080
>>    http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>>    ========================================
>>
>>
>>
> --
> ========================================
>
> Justin A. Lemkul
> Graduate Research Assistant
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
>
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