[gmx-users] problems with my PDB file

Joshua Adelman jadelman at berkeley.edu
Sat Apr 4 20:17:34 CEST 2009


Hi Jacob,

It looks like your pdb file does not contain any residue names, since  
you residue column is filled with the number "2" which I'm guessing  
isn't specified as a canonical residue. I'm not sure how you're  
generating this PDB file, but if you're doing it by hand, I would  
strongly recommend consulting the pdb formatting guide.

Josh


On Apr 4, 2009, at 11:03 AM, Jacob Harvey wrote:

> Dear All,
>
> I seem to be having issues with the pdb file I wish to use in  
> gromacs. I am trying to model this system using the OPLS-AA force  
> field however I get the error
>
> "Fatal error:
> Residue '' not found in residue topology database"
>
> I know what this error means but its not naming a specific residue  
> so I'm not sure where I am going wrong in terms of naming my atoms.  
> Does anyone see a way to fix this problem. Thank you.
>
> my pdb file:
>
> HETATM    1  CA      2     2      -4.438   3.334   2.988
> HETATM    2  CA      2     2       0.588   3.313   0.517
> HETATM    3  CA      2     2       5.476   3.310  -2.216
> HETATM    4  H4      2     2      -4.134   2.590   3.731
> HETATM    5  H5      2     2      -5.525   3.438   3.047
> HETATM    6  H6      2     2      -3.996   4.291   3.276
> HETATM    7  H7      2     2       1.480   3.111   1.117
> HETATM    8  H8      2     2      -0.266   3.349   1.200
> HETATM    9  H9      2     2       0.702   4.307   0.076
> HETATM   10  H10     2     2       6.375   3.525  -1.633
> HETATM   11  H11     2     2       4.648   3.862  -1.761
> HETATM   12  H12     2     2       5.628   3.709  -3.223
> HETATM   13  CA      2     2      -3.996   2.922   1.581
> HETATM   14  H14     2     2      -2.902   2.862   1.545
> HETATM   15  H15     2     2      -4.281   3.703   0.865
> HETATM   16  CA      2     2       0.394   2.242  -0.557
> HETATM   17  H17     2     2      -0.485   2.486  -1.167
> HETATM   18  H18     2     2       1.250   2.252  -1.242
> HETATM   19  CA      2     2       5.188   1.805  -2.247
> HETATM   20  H20     2     2       6.018   1.286  -2.742
> HETATM   21  H21     2     2       4.300   1.615  -2.864
> HETATM   22  CA      2     2      -4.587   1.582   1.127
> HETATM   23  H23     2     2      -5.682   1.638   1.161
> HETATM   24  H24     2     2      -4.298   0.792   1.828
> HETATM   25  CA      2     2       0.235   0.836   0.028
> HETATM   26  H26     2     2      -0.616   0.818   0.720
> HETATM   27  H27     2     2       1.117   0.581   0.625
> HETATM   28  CA      2     2       4.981   1.206  -0.852
> HETATM   29  H29     2     2       4.152   1.720  -0.350
> HETATM   30  H30     2     2       5.867   1.381  -0.234
> HETATM   31  CB      2     2      -4.129   1.184  -0.291
> HETATM   32  H32     2     2      -4.398   1.981  -0.995
> HETATM   33  H33     2     2      -3.041   1.101  -0.315
> HETATM   34  CB      2     2       0.034  -0.248  -1.048
> HETATM   35  H35     2     2      -0.851  -0.016  -1.650
> HETATM   36  H36     2     2       0.890  -0.242  -1.730
> HETATM   37  CB      2     2       4.702  -0.306  -0.866
> HETATM   38  H38     2     2       3.772  -0.519  -1.404
> HETATM   39  H39     2     2       5.504  -0.809  -1.420
> HETATM   40  CD2     2     2      -6.169  -1.597  -1.527
> HETATM   41  H41     2     2      -6.700   0.434  -1.148
> HETATM   42  CE1     2     2      -4.945  -2.182  -1.371
> HETATM   43  H43     2     2      -7.118  -1.976  -1.868
> HETATM   44  H44     2     2      -4.663  -3.203  -1.569
> HETATM   45  CD2     2     2      -1.054  -3.462   0.297
> HETATM   46  H46     2     2      -2.230  -1.848  -0.502
> HETATM   47  CE1     2     2       0.307  -3.542   0.421
> HETATM   48  H48     2     2      -1.844  -4.148   0.553
> HETATM   49  H49     2     2       0.898  -4.352   0.820
> HETATM   50  CD2     2     2       3.845  -1.970   2.274
> HETATM   51  H51     2     2       2.694  -1.850   0.476
> HETATM   52  CE1     2     2       5.086  -1.451   2.534
> HETATM   53  H53     2     2       3.156  -2.545   2.870
> HETATM   54  H54     2     2       5.660  -1.519   3.446
> HETATM   55  CG      2     2      -4.687  -0.117  -0.776
> HETATM   56  CG      2     2      -0.105  -1.625  -0.478
> HETATM   57  CG      2     2       4.625  -0.891   0.514
> HETATM   58  NE2     2     2      -5.991  -0.283  -1.145
> HETATM   59  ND1     2     2      -4.033  -1.258  -0.903
> HETATM   60  NE2     2     2      -1.303  -2.236  -0.278
> HETATM   61  ND1     2     2       0.892  -2.392  -0.062
> HETATM   62  NE2     2     2       3.558  -1.605   0.977
> HETATM   63  ND1     2     2       5.566  -0.779   1.434
>
> -- 
> --
> Jacob Harvey
>
> Graduate Student
>
> University of Massachusetts Amherst
>
> j.harv8 at gmail.com
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------------------------------------------------------------------------------------------------------
Joshua L. Adelman
Biophysics Graduate Group              Lab: 510.643.2159
218 Wellman Hall                                Fax: 510.642.7428
University of California, Berkeley     http://nature.berkeley.edu/~jadelman
Berkeley, CA 94720 USA                   jadelman at berkeley.edu
------------------------------------------------------------------------------------------------------



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