[gmx-users] Re: itp file

Jamie Seyed jamie.seyed at gmail.com
Mon Aug 10 21:23:57 CEST 2009


Hi Justin,
Ok.Thanks for comments and advice, I appreciate.../Jamie

On Mon, Aug 10, 2009 at 3:06 PM, Justin A. Lemkul <jalemkul at vt.edu> wrote:

>
>
> Jamie Seyed wrote:
>
>> Dear Vitaly and all,
>> I did copy my top file to a itp file and now for doing a md simulation I
>> need a box of water and so I was thinking to make a .gro file and use
>> editconf after that. But I did not know how, so I used the procedure by
>> Christopher Stiles (I used editconf to make a gro file from pdb and I did
>> not use my itp according to the procedure....) the gro file doesn't contain
>> velocities. Now when I run the grompp command, I got this Fatal
>>
>
> The .gro file will not contain velocities until you have run dynamics.
>
> error:
>> Group BAL not found in indexfile....
>>
>
> Then you have specified a molecule type that is not encompassed by your
> #included .itp files, or you have specified some special group in your .mdp
> file that doesn't exist without using a special index group.
>
> my questions are
>> 1) when I have my itp file and only I need a gro file (with velocities)
>> how can I make it??
>>
>
> I don't understand this question.
>
> 2) with Christopher Stiles method, why I got that fatal error and how I can
>> solve it to run a correct md simulation??
>>
>
> See above.  Without seeing the topology and .mdp file, it is hard to
> provide any more useful advice.
>
> I would seriously recommend running some simple tutorials on proteins (even
> if you don't care about proteins - these systems are very robust) to get a
> feel for procedures in Gromacs, how velocities are generated, how to use
> index files (if necessary), etc.
>
> -Justin
>
>  Many Thanks in advance/Jamie
>>
>>  On Mon, Aug 10, 2009 at 10:45 AM, Vitaly V. Chaban <vvchaban at gmail.com<mailto:
>> vvchaban at gmail.com>> wrote:
>>
>>    Jamie,
>>
>>    The best solution is to copy all the sections ([atoms], [bonds], etc)
>>    corresponding to each molecule into a separate file and then use
>>    "include" statement like in C language to attach these .itp files to
>>    .top file.
>>
>>    So you will have only the numbers of the each kind of molecules in
>>    your .top file. These manipulations are made only in the sake of
>>    future comfort. Below is an example how I do it.
>>
>>    Best,
>>      Vitaly
>>
>>    ===============topology  file==============
>>    #include "vvc.itp"
>>    #include "15x15x50.itp"
>>    #include "20x20x50.itp"
>>    #include "25x25x50.itp"
>>
>>
>>    [ system ]
>>    MWCNT with LIBF4 solution
>>
>>
>>    [ molecules ]
>>    15x15x50              1
>>    20x20x50              1
>>    25x25x50              1
>>    LI+                          7
>>    BF4                        7
>>    Methanol           254
>>
>>
>>    On Mon, Aug 10, 2009 at 4:38 PM, Jamie Seyed<jamie.seyed at gmail.com
>>    <mailto:jamie.seyed at gmail.com>> wrote:
>>     > Dear Vitaly,
>>     > Thanks for your reply. Justin helped me about that and I could
>>    get the
>>     > topology by x2top. In your last email you said after that I can
>>    export it to
>>     > the .itp right? Would you please explain more about that, I mean
>>    for example
>>     > should I change it manually to be look like a itp file or is that
>>    something
>>     > else?? I appreciate your help...
>>     > Thanks a lot/Jamie
>>     >
>>     > On Mon, Aug 10, 2009 at 2:18 AM, Vitaly V. Chaban
>>    <vvchaban at gmail.com <mailto:vvchaban at gmail.com>>
>>     > wrote:
>>     >>
>>     >> Jamie,
>>     >>
>>     >> What error messages do they provide? What force fields do you use?
>>     >> There were many rumors about these utilities here in the list
>>    but they
>>     >> seem to be workable in most cases.
>>     >>
>>     >> Vitaly
>>     >>
>>     >> On Mon, Aug 10, 2009 at 1:39 AM, Jamie
>>    Seyed<jamie.seyed at gmail.com <mailto:jamie.seyed at gmail.com>> wrote:
>>     >> > Hi Vitaly,
>>     >> > Actually it doe's not work. I tried but I got error messages
>>    for both
>>     >> > cases.
>>     >> > Actually the structure is big and contains a c60 part. Do you
>>    have any
>>     >> > advice? I could not find in the topology part of archive...
>>     >> > Thanks/Jamie
>>     >> >
>>     >> >
>>     >> > On Sun, Aug 9, 2009 at 2:54 AM, Vitaly V. Chaban
>>    <vvchaban at gmail.com <mailto:vvchaban at gmail.com>>
>>
>>     >> > wrote:
>>     >> >>
>>     >> >> Hi,
>>     >> >>
>>     >> >> Did you try pdb2gmx, x2top to make .top and then export to .itp?
>>     >> >> Sometimes also it appears easier to make the topology by hand
>>    if your
>>     >> >> structure is not big. Please see the topologies for some
>>    molecules in
>>     >> >> the gromacs archive to use them as an example.
>>     >> >>
>>     >> >> Vitaly
>>     >> >>
>>     >> >> >
>>     >> >> > Dear gmx users,
>>     >> >> > I need some help for generating a itp file for my molecular
>>     >> >> > structure. I
>>     >> >> > am
>>     >> >> > using molden and now I have the xyz and pdb files. I read
>>    through
>>     >> >> > mailing
>>     >> >> > list but seems I should provide itp myself. But I don't
>>    know the
>>     >> >> > right
>>     >> >> > way
>>     >> >> > to do that. Is it using molden and find one-by-one bond
>>    sand angles
>>     >> >> > and
>>     >> >> > dihedrals... . If I have already the itp file for a part of
>>    molecule
>>     >> >> > is
>>     >> >> > it
>>     >> >> > only to add the information of new atoms to the old itp
>>    file?? I will
>>     >> >> > really
>>     >> >> > appreciate it if someone gives me better ideas and more
>> related
>>     >> >> > information
>>     >> >> > to make my itp file.
>>     >> >> >
>>     >> >> > Many Thanks in Advance,
>>     >> >> > Jamie
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>>     >>
>>     >>
>>     >>
>>     >> --
>>     >> Vitaly V. Chaban, Ph.D. (ABD)
>>     >> School of Chemistry
>>     >> V.N. Karazin Kharkiv National University
>>     >> Svoboda sq.,4, Kharkiv 61077, Ukraine
>>     >> email: chaban at univer.kharkov.ua
>>    <mailto:chaban at univer.kharkov.ua>,vvchaban at gmail.com
>>    <mailto:vvchaban at gmail.com>
>>     >> skype: vvchaban, cell.: +38-097-8259698
>>     >> http://www-rmn.univer.kharkov.ua/chaban.html
>>     >> ===================================
>>     >> !!! Looking for a postdoctoral position !!!
>>     >> ===================================
>>     >
>>     >
>>
>>
>>
>> ------------------------------------------------------------------------
>>
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>
> --
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
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