[gmx-users] bad bond in polymer

Justin A. Lemkul jalemkul at vt.edu
Fri Aug 14 20:20:28 CEST 2009


All of your residue numbers are one, so I think pdb2gmx is trying to make them 
all one residue.  Try giving each unit a sequential residue number.

-Justin

nicegromacs wrote:
> Hi Justin,
> 
> the pdb file:
> 
> HETATM    1  CAA DRG     1      83.043  46.127  36.820
> HETATM    2  CAB DRG     1      82.411  45.765  35.421
> HETATM    3  CAC DRG     1      81.495  46.822  34.598
> HETATM    4  CAD DRG     1      80.621  46.369  33.071
> HETATM    5  CAE DRG     1      79.305  47.302  32.172
> HETATM    6  CAF DRG     1      77.939  46.749  31.124
> HETATM    7  CAG DRG     1      76.264  47.439  30.612
> HETATM    8  CAH DRG     1      74.636  46.805  29.962
> HETATM    9  CAI DRG     1      72.805  47.674  30.179
> HETATM   10  CAJ DRG     1      73.794  48.838  31.123
> HETATM   11  OAK DRG     1      74.815  49.342  30.292
> HETATM   12  OAL DRG     1      74.592  48.143  32.194
> HETATM   13  CAM DRG     1      71.093  47.685  29.486
> HETATM   14  CAN DRG     1      71.598  47.212  28.208
> HETATM   15  OAO DRG     1      72.440  48.255  27.735
> HETATM   16  OAP DRG     1      72.374  46.050  28.399
> HETATM   17  CAA DRG     1      72.093  46.197  36.714
> HETATM   18  CAB DRG     1      71.418  45.759  35.400
> HETATM   19  CAC DRG     1      71.084  46.901  34.417
> HETATM   20  CAD DRG     1      70.620  46.251  33.139
> HETATM   21  CAE DRG     1      70.650  47.255  32.000
> HETATM   22  CAF DRG     1      69.655  46.512  30.955
> HETATM   23  CAG DRG     1      69.081  47.504  29.843
> HETATM   24  CAH DRG     1      67.208  47.518  29.256
> HETATM   25  CAI DRG     1      65.298  47.601  29.691
> HETATM   26  CAJ DRG     1      65.629  48.362  30.827
> HETATM   27  OAK DRG     1      65.140  49.590  30.429
> HETATM   28  OAL DRG     1      64.837  47.815  31.709
> HETATM   29  CAM DRG     1      63.041  47.557  29.333
> HETATM   30  CAN DRG     1      62.808  47.023  27.931
> HETATM   31  OAO DRG     1      63.130  48.142  27.185
> HETATM   32  OAP DRG     1      63.560  45.894  27.706
> HETATM   33  CAA DRG     1      61.174  46.188  36.668
> HETATM   34  CAB DRG     1      60.510  45.777  35.344
> HETATM   35  CAC DRG     1      60.221  46.928  34.352
> HETATM   36  CAD DRG     1      59.724  46.337  32.998
> HETATM   37  CAE DRG     1      60.252  47.307  31.985
> HETATM   38  CAF DRG     1      61.089  46.606  30.881
> HETATM   39  CAG DRG     1      60.868  47.564  29.740
> HETATM   40  CAH DRG     1      58.621  47.652  29.253
> HETATM   41  CAI DRG     1      56.328  47.703  29.755
> HETATM   42  CAJ DRG     1      55.914  48.516  30.963
> HETATM   43  OAK DRG     1      55.050  49.545  30.403
> HETATM   44  OAL DRG     1      54.945  47.724  31.565
> HETATM   45  CAM DRG     1      54.420  47.478  29.367
> HETATM   46  CAN DRG     1      53.587  47.142  27.983
> HETATM   47  OAO DRG     1      52.713  48.009  27.325
> HETATM   48  OAP DRG     1      53.396  45.805  27.517
> HETATM   49  CAA DRG     1      50.257  46.173  36.646
> HETATM   50  CAB DRG     1      49.673  45.778  35.270
> HETATM   51  CAC DRG     1      49.360  46.939  34.273
> HETATM   52  CAD DRG     1      49.270  46.480  32.735
> HETATM   53  CAE DRG     1      50.289  47.086  31.602
> HETATM   54  CAF DRG     1      51.813  46.713  31.234
> HETATM   55  CAG DRG     1      52.487  47.720  29.966
> HETATM   56  CAH DRG     1      50.870  47.989  29.178
> HETATM   57  CAI DRG     1      48.982  47.816  29.191
> HETATM   58  CAJ DRG     1      47.901  48.319  30.192
> HETATM   59  OAK DRG     1      47.004  49.132  29.503
> HETATM   60  OAL DRG     1      46.963  47.171  30.588
> HETATM   61  CAM DRG     1      47.752  46.922  28.157
> HETATM   62  CAN DRG     1      46.372  47.149  27.020
> HETATM   63  OAO DRG     1      45.018  47.950  26.877
> HETATM   64  OAP DRG     1      46.019  46.082  26.137
> 
> 
> --------------------------------------------------
> From: "Justin A. Lemkul" <jalemkul at vt.edu>
> Sent: Friday, August 14, 2009 12:14 PM
> To: "Gromacs Users' List" <gmx-users at gromacs.org>
> Subject: Re: [gmx-users] bad bond in polymer
> 
>>
>>
>> nicegromacs wrote:
>>> I follow some of the step of 
>>> http://oldwww.gromacs.org/pipermail/gmx-users/2009-March/040125.html 
>>> (I omitted the change in the .hdb file because I don't have explicit 
>>> hydrogens). Well, I build a polymer with 3 units. The head (H), one 
>>> repeat unit (R)and tail (T) for the polymer (H-R-T) and this gives 
>>> ok, but when I add one more repetitive unit (H-R-R-T) I get the 
>>> following error:
>>> Program pdb2gmx, VERSION 3.3.3
>>> Source code file: ../../../../src/kernel/pgutil.c, line: 87
>>> Fatal error:
>>> Atom CAM not found in residue 1083339441 while adding improper
>>>
>>> -------------------------------------------------------
>>>
>>
>> It looks like pdb2gmx is finding some kind of undefined residue.  Can 
>> you post the .pdb file?
>>
>> -Justin
>>
>>> "Stop Drinking My Beer !" (The Amps)
>>>
>>>
>>>>
>>>>
>>>> nicegromacs wrote:
>>>>
>>>>>   CAG -CAM
>>>>
>>>> Defining a bond to a previous residue will certainly fail for at 
>>>> least one terminus of your polymer chain.  You may find this post 
>>>> useful:
>>>>
>>>> http://oldwww.gromacs.org/pipermail/gmx-users/2009-March/040125.html
>>>>
>>>> If things still aren't working, please post the structure file, as 
>>>> well, and identify the bond that is being formed that shouldn't.
>>>>
>>>> -Justin
>>>>
>>>> -- 
>>>> ========================================
>>>>
>>>> Justin A. Lemkul
>>>> Ph.D. Candidate
>>>> ICTAS Doctoral Scholar
>>>> Department of Biochemistry
>>>> Virginia Tech
>>>> Blacksburg, VA
>>>> jalemkul[at]vt.edu | (540) 231-9080
>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>>
>>>> ========================================
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>>>
>>
>> -- 
>> ========================================
>>
>> Justin A. Lemkul
>> Ph.D. Candidate
>> ICTAS Doctoral Scholar
>> Department of Biochemistry
>> Virginia Tech
>> Blacksburg, VA
>> jalemkul[at]vt.edu | (540) 231-9080
>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>> ========================================
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>>
> 

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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