[gmx-users] Why NaCl and KCl solution?

[Lucio Ricardo Montero Valenzuela]

The crystalization chemical conditions should appear described in the header of
your PDB file, there you can see if there were, for example, sodium ions.

Lucio Montero
PhD. Candidate
Instituto de Biotecnologia, UNAM
Mexico

Mensaje citado por "Justin A. Lemkul" <jalemkul at vt.edu>:


>
> Chih-Ying Lin wrote:
> > HI
> >
> >
> >
> > 1. Would you please tell me the reason that "generally people use NaCl
> > or KCl in the solution with protein"?
> >
> >
> > 2. Why does PDB file of the protein NOT Carrying ions?
> >
> >
>
> PDB structures do not contain information about the properties of the solvent
> or
> precipitant used to obtain the structure.  Occasionally some additive
> (glycerol,
> etc.) will be present since it is bound to the protein.  Some background on
> X-ray crystallography or NMR will explain structure determination.
>
> -Justin
>
> >
> > Thank you
> > Lin
> > _______________________________________________
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>
> --
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
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Lucio Ricardo Montero Valenzuela
Instituto de Biotecnologia, UNAM
Departamento de Biologia Molecular de Plantas
Av. Universidad 2001, Col. Chamilpa
Cuernavaca 62210
Mexico

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