[gmx-users] gromacs doubt

manjula kasinathan manjubfs at gmail.com
Sat May 8 12:40:43 CEST 2010


hi all

      i'm doing simulation for complex structure (protein length around
1020). while running mdrun for position restrain it shows error like this



Step 434, time 0.868 (ps)  LINCS WARNING
relative constraint deviation after LINCS:
rms 1818.708939, max 108974.859375 (between atoms 1917 and 1918)
bonds that rotated more than 30 degrees:
 atom 1 atom 2  angle  previous, current, constraint length
   2873   2875   90.0    0.1503   0.1088      0.1000
   1930   1932   66.8    0.1764   0.2060      0.1470
   1930   1931   69.3    0.1413   0.2511      0.1000
   1925   1927   33.2    0.1241   0.1563      0.1250
   1925   1926   54.0    0.1238   0.1773      0.1250
   1924   1925   50.0    0.1514   4.6004      0.1530
   1923   1924  156.4    0.2264  20.8825      0.1530
   1921   1923  139.8    0.2458  45.8946      0.1470
   1921   1922  116.5    0.1880  34.8778      0.1000
   1919   1920   42.5    0.2799 206.5039      0.1230
   1917   1919   83.9    0.3952 1749.0353      0.1530
   1917   1918   90.2    0.7744 16673.3066      0.1530
   1915   1917   96.0    0.5866 1624.9022      0.1470
   1915   1916   93.7    0.2045 470.1029      0.1000
   1913   1915   88.9    0.3074 522.5389      0.1330
   1913   1914  115.2    0.3731  11.2578      0.1230
   1912   1913  117.0    0.1173  11.5079      0.1530
   1910   1912   91.5    0.1471  80.4026      0.1470
   1910   1911  128.1    0.1822  34.9078      0.1000
   1908   1910   90.1    0.1345 6296.6333      0.1330
   1908   1909   89.0    0.1590 6080.4458      0.1230
   1905   1906   90.2    0.0989 642.7140      0.1000
   1904   1907   90.0    0.1530 7623.7417      0.1530
   1904   1905   90.0    0.1416 7605.1113      0.1430
   1903   1908   89.3    0.1776 7638.7241      0.1530
   1903   1904   90.0    0.1665 4890.0454      0.1530
   1901   1903   91.3    0.1556 8921.3604      0.1470
   1901   1902   65.9    0.1047  59.7358      0.1000
   1899   1900  176.1    0.1212 121.1425      0.1230
   1894   1899   34.3    0.1521  87.4365      0.1530
   1894   1895   90.5    0.1513  11.8270      0.1530
   1892   1894  107.0    0.1453  12.1096      0.1470
   1892   1893   76.7    0.0988   1.3052      0.1000
   1890   1892   49.3    0.1318   1.3995      0.1330
   1890   1891   57.8    0.1215   0.0482      0.1230
   1886   1890   34.4    0.1511   0.0948      0.1530
   1437   1438   69.2    0.0989   0.2078      0.1000
   1436   1437   89.7    0.1345   0.2161      0.1360
   1434   1436   62.0    0.1375   0.0617      0.1390
   1434   1435   39.0    0.1078   0.1438      0.1090
   1432   1436   91.0    0.1375   2.8230      0.1390
   1432   1433   90.7    0.1078   1.1621      0.1090
   1430   1434  102.3    0.1375   0.2004      0.1390
   1430   1431   94.7    0.1078   0.1235      0.1090
   1428   1432   89.2    0.1375   2.1372      0.1390
   1428   1429   91.2    0.1078   1.6125      0.1090
   1427   1430   87.0    0.1375   0.9626      0.1390
   1427   1428   92.1    0.1375   1.8618      0.1390
   1426   1427   93.3    0.1514   0.8468      0.1530
   8326   8328   89.9    0.4409   1.1304      0.1000
   8326   8327   90.1    0.4916   0.2944      0.1000
   8324   8326   90.3    0.1156   0.2084      0.1330
   8324   8325   88.6    0.1134   0.1387      0.1230
   8323   8324   48.0    0.1226   0.2008      0.1530
   8322   8323   31.2    0.1656   0.1504      0.1530
   8320   8321   38.4    0.1375   0.0957      0.1000
   8091   8092   38.4    0.0991   0.1000      0.1000
   8001   8002   90.0    0.0982   0.1635      0.1000
   7838   7840   90.1    0.1018   0.1239      0.1000
   6599   6600   38.1    0.0990   0.1002      0.1000
   5583   5585   89.9    0.0983   0.1465      0.1000
   5524   5525   38.7    0.0992   0.1004      0.1000

t = 0.868 ps: Water molecule starting at atom 36658 can not be settled.
Check for bad contacts and/or reduce the timestep.
Wrote pdb files with previous and current coordinates


wat should i do.i carried out mdrun for 10ps nsteps=5000. can any one help
me to overcome this error. thanks in advance.



-- 
With Regards,

Manjulakasinathan
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