[gmx-users] rerun trajectory does not match input file

John Shultz jackygrahamez at gmail.com
Sat May 22 16:31:30 CEST 2010


Thanks Justin!

On Sat, May 22, 2010 at 10:12 AM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
>
>
> John Shultz wrote:
>>
>> Why do I get this message saying the group is empty?
>>
>>
>>
>> Analysing residue names:
>> Opening library file /usr/local/gromacs/share/gromacs/top/aminoacids.dat
>> There are: 11350      OTHER residues
>> There are:   244    PROTEIN residues
>> There are:     0        DNA residues
>> Analysing Protein...
>> Analysing Other...
>>
>>  0 System              : 37770 atoms
>>  1 Protein             :  3848 atoms
>>  2 Protein-H           :  1980 atoms
>>  3 C-alpha             :   244 atoms
>>  4 Backbone            :   732 atoms
>>  5 MainChain           :   974 atoms
>>  6 MainChain+Cb        :  1206 atoms
>>  7 MainChain+H         :  1198 atoms
>>  8 SideChain           :  2650 atoms
>>  9 SideChain-H         :  1006 atoms
>>  10 Prot-Masses         :  3848 atoms
>>  11 Non-Protein         : 33922 atoms
>>  12 LIG                 :    35 atoms
>>  13 SOL                 : 33807 atoms
>>  14 Na                  :    46 atoms
>>  15 Cl                  :    34 atoms
>>  16 Other               : 33922 atoms
>>
>>  nr : group       !   'name' nr name   'splitch' nr    Enter: list groups
>>  'a': atom        &   'del' nr         'splitres' nr   'l': list residues
>>  't': atom type   |   'keep' nr        'splitat' nr    'h': help
>>  'r': residue         'res' nr         'chain' char
>>  "name": group        'case': case sensitive           'q': save and quit
>>
>>> 12 & 1
>>
>> Copied index group 12 'LIG'
>> Copied index group 1 'Protein'
>> Merged two groups with AND: 35 3848 -> 0
>> Group is empty
>>
>>> 1 & 12
>>
>> Copied index group 1 'Protein'
>> Copied index group 12 'LIG'
>> Merged two groups with AND: 3848 35 -> 0
>> Group is empty
>>
>
> The use of & tells make_ndx to write out a group that is the intersection of
> these two.  If there are no overlaps, the group is empty.  What you want is
> the "or" operator:
>
> 12 | 1
>
> -Justin
>
>>
>>
>>
>> On Sat, May 22, 2010 at 9:57 AM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
>>>
>>> John Shultz wrote:
>>>>
>>>> Justin,
>>>>
>>>> I finally got a chance to get back to this. I read the make_ndx
>>>> instructions. Unless I am mistaken, I guess that is what you use to
>>>> merge groups.
>>>>
>>> Yep, that's the purpose of make_ndx.
>>>
>>> -Justin
>>>
>>>> Thanks,
>>>>
>>>> Jack
>>>>
>>>> On Thu, May 13, 2010 at 9:15 AM, Justin A. Lemkul <jalemkul at vt.edu>
>>>> wrote:
>>>>>
>>>>> John Shultz wrote:
>>>>>>
>>>>>> Thank you Justin, is there a way I can choose both Protein LIG? It has
>>>>>> the following options for me
>>>>>>
>>>>> You need to make a custom group that merges these two.
>>>>>
>>>>> -Justin
>>>>>
>>>>>> Reading toplogy and shit from md.tpr
>>>>>> Reading file md.tpr, VERSION 4.0.5 (single precision)
>>>>>> 50000 steps (100 ps) remaining from first run.
>>>>>> Opening library file
>>>>>> /usr/local/gromacs/share/gromacs/top/aminoacids.dat
>>>>>> Group     0 (      System) has 37770 elements
>>>>>> Group     1 (     Protein) has  3848 elements
>>>>>> Group     2 (   Protein-H) has  1980 elements
>>>>>> Group     3 (     C-alpha) has   244 elements
>>>>>> Group     4 (    Backbone) has   732 elements
>>>>>> Group     5 (   MainChain) has   974 elements
>>>>>> Group     6 (MainChain+Cb) has  1206 elements
>>>>>> Group     7 ( MainChain+H) has  1198 elements
>>>>>> Group     8 (   SideChain) has  2650 elements
>>>>>> Group     9 ( SideChain-H) has  1006 elements
>>>>>> Group    10 ( Prot-Masses) has  3848 elements
>>>>>> Group    11 ( Non-Protein) has 33922 elements
>>>>>> Group    12 (         LIG) has    35 elements
>>>>>> Group    13 (         SOL) has 33807 elements
>>>>>> Group    14 (          Na) has    46 elements
>>>>>> Group    15 (          Cl) has    34 elements
>>>>>> Group    16 (       Other) has 33922 elements
>>>>>> Select a group:
>>>>>>
>>>>>>
>>>>>> On Thu, May 13, 2010 at 9:02 AM, Justin A. Lemkul <jalemkul at vt.edu>
>>>>>> wrote:
>>>>>>>
>>>>>>> John Shultz wrote:
>>>>>>>>
>>>>>>>> I am trying to rerun a simulation using this command
>>>>>>>> mdrun -rerun -v -deffnm md
>>>>>>>>
>>>>>>>> I think I must have made a mistake when I prepared the original mdp
>>>>>>>> file because I get this message
>>>>>>>> Number of atoms in trajectory (3883) does not match the run input
>>>>>>>> file
>>>>>>>> (37770)
>>>>>>>>
>>>>>>>> I have these files in my directory
>>>>>>>> Complex.top  ener.edr  job.xml  md.cpt  md.edr  md.gro  md.log
>>>>>>>>  md.mdp
>>>>>>>>  md_prev.cpt  md.tpr  md.trr  md.xtc  traj.trr
>>>>>>>>
>>>>>>>> Here is my mdp file, should I remove entries for xtc_grps and
>>>>>>>> energygrps to avoid this issue?
>>>>>>>>
>>>>>>> You can, but it won't fix anything.  Your original .xtc file saved
>>>>>>> only
>>>>>>> the
>>>>>>> coordinates of the Protein and LIG (per your output options), but
>>>>>>> your
>>>>>>> .tpr
>>>>>>> file has all of these atoms, regardless of what you choose to save.
>>>>>>>  You
>>>>>>> can, however, create a .tpr file that has a just these atoms by
>>>>>>> passing
>>>>>>> your
>>>>>>> original .tpr file to tpbconv, using a suitable index group.
>>>>>>>
>>>>>>> -Justin
>>>>>>>
>>>>>>>> integrator = md
>>>>>>>> nsteps = 50000
>>>>>>>> dt = 0.002
>>>>>>>> nstvout = 5000
>>>>>>>> nstlog = 500
>>>>>>>> nstenergy = 250
>>>>>>>> nstxtcout = 5000
>>>>>>>> nstxout = 5000
>>>>>>>> xtc_grps = Protein LIG
>>>>>>>> energygrps = Protein  SOL
>>>>>>>> constraints = all-bonds
>>>>>>>> nstcomm = 1
>>>>>>>> ns_type = grid
>>>>>>>> rlist = 1.2
>>>>>>>> rcoulomb = 1.1
>>>>>>>> rvdw = 1.0
>>>>>>>> vdwtype = shift
>>>>>>>> rvdw-switch = 0.9
>>>>>>>> coulombtype = PME-Switch
>>>>>>>> Tcoupl = v-rescale
>>>>>>>> tau_t = 0.1 0.1
>>>>>>>> tc-grps = protein non-protein
>>>>>>>> ref_t = 300 300
>>>>>>>> Pcoupl = parrinello-rahman
>>>>>>>> PcOupltype = isotropic
>>>>>>>> tau_p = 0.5
>>>>>>>> compressibility = 4.5e-5
>>>>>>>> ref_p = 1.0
>>>>>>>> gen_vel = yes
>>>>>>>> lincs-iter = 2
>>>>>>>> DispCorr = EnerPres
>>>>>>>> optimize_fft = yes
>>>>>>>> gen_seed = 805087
>>>>>>>
>>>>>>> --
>>>>>>> ========================================
>>>>>>>
>>>>>>> Justin A. Lemkul
>>>>>>> Ph.D. Candidate
>>>>>>> ICTAS Doctoral Scholar
>>>>>>> MILES-IGERT Trainee
>>>>>>> Department of Biochemistry
>>>>>>> Virginia Tech
>>>>>>> Blacksburg, VA
>>>>>>> jalemkul[at]vt.edu | (540) 231-9080
>>>>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>>>>>
>>>>>>> ========================================
>>>>>>> --
>>>>>>> gmx-users mailing list    gmx-users at gromacs.org
>>>>>>> http://lists.gromacs.org/mailman/listinfo/gmx-users
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>>>>>>>
>>>>> --
>>>>> ========================================
>>>>>
>>>>> Justin A. Lemkul
>>>>> Ph.D. Candidate
>>>>> ICTAS Doctoral Scholar
>>>>> MILES-IGERT Trainee
>>>>> Department of Biochemistry
>>>>> Virginia Tech
>>>>> Blacksburg, VA
>>>>> jalemkul[at]vt.edu | (540) 231-9080
>>>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>>>
>>>>> ========================================
>>>>> --
>>>>> gmx-users mailing list    gmx-users at gromacs.org
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>>>>>
>>> --
>>> ========================================
>>>
>>> Justin A. Lemkul
>>> Ph.D. Candidate
>>> ICTAS Doctoral Scholar
>>> MILES-IGERT Trainee
>>> Department of Biochemistry
>>> Virginia Tech
>>> Blacksburg, VA
>>> jalemkul[at]vt.edu | (540) 231-9080
>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>
>>> ========================================
>>> --
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>>>
>>
>
> --
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> MILES-IGERT Trainee
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
> --
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> http://lists.gromacs.org/mailman/listinfo/gmx-users
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> or send it to gmx-users-request at gromacs.org.
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