[gmx-users] Reg: NPT Equilibration of water

Mark Abraham Mark.Abraham at anu.edu.au
Fri Oct 29 10:36:49 CEST 2010


On 29/10/2010 6:39 PM, vinothkumar mohanakrishnan wrote:
> I am using the semiisotropic pressure scaling because i want the box 
> size to remain the same as that of the original box size in X and Y 
> axis and want to change it only on the Z axis. I am doing this because 
> at a latter stage i want to create an interface with organic solvent 
> where i need the cross sections (X and Y axis length) of both the box 
> should be the same.
>
> what i should do now  get the pressure of 1 bar?

7 +/- 500 *is* approximately 1 bar, and hardly any better an 
approximation than 1 +/- 500. If you want lower fluctuations, use a 
larger system and run for a much longer time. Or, since you'll have to 
re-equilibrate once you combine the solvent boxes, don't bother.

Mark
>
> Has no one has got the pressure of water to be close to 1 bar in 
> GROMACS till now?
>
> Regards
> Vinoth
>
> On Fri, Oct 29, 2010 at 12:54 PM, David van der Spoel 
> <spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>> wrote:
>
>     On 2010-10-29 09.08, vinothkumar mohanakrishnan wrote:
>
>
>         Hi Mark
>
>         I read the link before posting the question. even though the
>         fluctation
>         is between 500-600 (as said in the link) bar the average
>         pressure is
>         around 7.4 bar. my concern is the average pressure?. Because
>         at a latter
>         stage i am going to combine this water box with another
>         organic solvent
>         and i want to avoid any complications there.
>
>
>     Think again of what you just wrote. Your value is 7 +/- 500. In
>     fact according to normal statistical rules you should round the
>     value of 7 to 0. If you want more accurate number you could
>     increase the box size by a factor of 100.
>
>     By the way, why are you using semiisotropic pressure scaling in a
>     water box?
>
>
>         Regards
>         Vinoth
>
>         On Fri, Oct 29, 2010 at 12:22 PM, Mark Abraham
>         <Mark.Abraham at anu.edu.au <mailto:Mark.Abraham at anu.edu.au>
>         <mailto:Mark.Abraham at anu.edu.au
>         <mailto:Mark.Abraham at anu.edu.au>>> wrote:
>
>            On 29/10/2010 5:48 PM, vinothkumar mohanakrishnan wrote:
>
>                Hi Gromacians
>
>                I want to do equilibration of water (spc model) first
>         in the NVT
>                ensemble and then in the NPT ensemble to maintain a
>         temperature
>                of 300K and a pressure of 1 bar respectively. The NVT
>                equilibration works fine and the average temperature
>         turns out
>                to be 299.229 K.
>
>                Energy                      Average       RMSD      
>          Fluct.
>                      Drift            Tot-Drift
>                ------------------------------
>                -------------------------------------------------
>                Temperature                 299.229    10.3092    10.2463
>                  0.0393873    3.93877
>                Heat Capacity Cv:       12.494 J/mol K (factor =
>         0.00118698)
>
>                  when i do NPT equilibration i am not getting the desired
>                pressure as 1 bar or atleast close to 1 bar (between
>         1-1.4 bar).
>                In the mdp file i used semiisotropic pressure coupling type
>                because i want to fix the length of the box same on two
>         axis as
>                that of the original box size and want to change it
>         only on one
>                axis. can any one tell me why iam not getting the desired
>                pressure of 1 bar?.
>
>
>            This looks normal for a smallish water system over 100ps. See
>         http://www.gromacs.org/Documentation/Terminology/Pressure
>
>            Mark
>
>
>
>                Energy                      Average       RMSD     Fluct.
>                  Drift  Tot-Drift
>              
>          -------------------------------------------------------------------------------
>                Pressure (bar)               7.4339    574.052    573.574
>                0.812129    81.2138
>
>                Given below is my mdp file (NPT equilibration). any help is
>                highly appreciated.
>
>                title           = DCE NVT equilibration
>                cpp           = usr/bin/cpp
>                integrator   = md
>                nsteps       = 100000
>                dt              = 0.001
>                nstxout       = 100
>                nstvout       = 100
>                nstenergy   = 100
>                nstlog        = 100
>                ns_type     = grid
>                nstlist        = 1
>                rlist            = 1.0
>                coulombtype    = PME
>                rcoulomb         = 1.0
>                vdwtype          = Cut-off
>                rvdw               = 1.0
>                pme_order       = 4
>                fourierspacing  = 0.16
>                pbc                 = xyz
>                tcoupl              = V-rescale
>                tc-grps             = system
>                tau_t                = 0.1
>                ref_t                 = 300
>                pcoupl              = berendsen
>                pcoupltype        = semiisotropic
>                tau_p                = 0.5
>                ref_p                 = 1.0 1.0
>                compressibility   = 0.0 4.5e-5
>                DispCorr            = Enerpres
>                gen_vel             = yes
>                gen_temp          = 300
>                gen_seed          = 173529
>
>                Regards
>                Vinoth
>
>
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>     -- 
>     David van der Spoel, Ph.D., Professor of Biology
>     Dept. of Cell & Molec. Biol., Uppsala University.
>     Box 596, 75124 Uppsala, Sweden. Phone:  +46184714205.
>     spoel at xray.bmc.uu.se <mailto:spoel at xray.bmc.uu.se>
>     http://folding.bmc.uu.se
>
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