[gmx-users] links warnangles

Justin A. Lemkul jalemkul at vt.edu
Wed Feb 2 15:12:44 CET 2011



vferrario at units.it wrote:
> Seems that the new definition I've found works, but it is still unclear 
> to me why...
> 

There was a post long ago that I seem to remember reported the same problem. 
Since there is no true bond between H and C, the H position is basically 
triangulated by all of the other atoms.  There should not be a bond between H 
and C, since it should be constrained, yielding too many degrees of freedom. 
Seems to me that these rigid Gromos96 solvent models are a little strange.

-Justin

> Valerio
> 
> 
> vferrario at units.it ha scritto:
> 
>> The molecule is rigid because it's all constrained and the coordinates 
>> of the single molecule seem to be ok:
>>
>> CLF
>>     5
>>     1CLF    CCl    1   3.041   1.571   0.924
>>     1CLF    HCl    2   3.001   1.637   0.845
>>     1CLF    CL1    3   3.071   1.661   1.073
>>     1CLF    CL2    4   3.186   1.493   0.867
>>     1CLF    CL3    5   2.916   1.452   0.963
>>    0.50000   0.50000   0.50000
>>
>> I've found another CHCL3 model on the GROMACS website by user 
>> contribution, but the difinition is closed to mine, exept for the 
>> hydrogen atom which is not considered as an independent particle but 
>> is considered with the carbon:
>>
>> [ atomtypes ]
>>
>> ;type    mass    charge       ptype          sigma      epsilon
>>
>>  CH      12.01100   0.420       A        3.80000e-01  3.26944e-01
>>
>>  CLCL3   35.45300  -0.140       A        3.47000e-01  1.25604e+00
>>
>>
>>
>> [ moleculetype ]
>>
>> ; name  nrexcl
>>
>> CCl3      3
>>
>>
>>
>> [ atoms ]
>>
>> ;   nr   type   resnr  residu  atom    cgnr    charge
>>
>>     1  CH        1      CCl4    CT1     1        0.420
>>
>>     2  CLCL3     1    CCl4    Cl2    1    -0.140
>>
>>     3  CLCL3     1    CCl4    Cl3    1    -0.140
>>
>>     4  CLCL3     1    CCl4    Cl4    1    -0.140
>>
>>
>>
>>
>>
>> [ constraints ]
>>
>> ;  ai    aj funct        b0
>>
>>     1     2     1     0.17580
>>
>>     1     3     1     0.17580
>>
>>     1     4     1     0.17580
>>
>>     2     3     1     0.2902831
>>
>>     2     4     1     0.2902831
>>
>>     3     4     1     0.2902831
>>
>>
>> Probably is the definition of that single hydrogen that cause 
>> problems, I'll check this new definition.
>>
>> Valerio
>>
>>
>> "Justin A. Lemkul" <jalemkul at vt.edu> ha scritto:
>>
>>>
>>>
>>> Justin A. Lemkul wrote:
>>>>
>>>>
>>>> vferrario at units.it wrote:
>>>>> I've just tried with a single CHCL3 molecule, here's the output:
>>>>>
>>>>> Back Off! I just backed up md.log to ./#md.log.2#
>>>>> Getting Loaded...
>>>>> Reading file clfmin.tpr, VERSION 4.0.7 (single precision)
>>>>> Loaded with Money
>>>>>
>>>>> NNODES=2, MYRANK=1, HOSTNAME=biohazard
>>>>> NODEID=1 argc=6
>>>>> Making 1D domain decomposition 2 x 1 x 1
>>>>>
>>>>> Step -1, time -0.001 (ps)  LINCS WARNING
>>>>> relative constraint deviation after LINCS:
>>>>> rms 301.323604, max 324.810791 (between atoms 1 and 3)
>>>>> bonds that rotated more than 90 degrees:
>>>>> atom 1 atom 2  angle  previous, current, constraint length
>>>>>
>>>>> Back Off! I just backed up step-1b_n1.pdb to ./#step-1b_n1.pdb.2#
>>>>>
>>>>> Back Off! I just backed up step-1c_n1.pdb to ./#step-1c_n1.pdb.2#
>>>>> Wrote pdb files with previous and current coordinates
>>>>>
>>>>> Back Off! I just backed up traj.trr to ./#traj.trr.1#
>>>>>
>>>>> Back Off! I just backed up ener.edr to ./#ener.edr.2#
>>>>> Steepest Descents:
>>>>>  Tolerance (Fmax)   =  2.50000e+00
>>>>>  Number of steps    =       100000
>>>>> Step=    0, Dmax= 1.0e-02 nm, Epot=  0.00000e+00 Fmax= 0.00000e+00, 
>>>>> atom= 0
>>>>>
>>>>> writing lowest energy coordinates.
>>>>>
>>>>> Back Off! I just backed up clfmin.gro to ./#clfmin.gro.1#
>>>>>
>>>>> Steepest Descents converged to Fmax < 2.5 in 1 steps
>>>>> Potential Energy  =  0.0000000e+00
>>>>> Maximum force     =  0.0000000e+00 on atom 0
>>>>> Norm of force     =  0.0000000e+00
>>>>>
>>>>> NOTE: 7 % of the run time was spent communicating energies,
>>>>>     you might want to use the -nosum option of mdrun
>>>>>
>>>>>
>>>>> gcq#320: "Do You Have Sex Maniacs or Schizophrenics or 
>>>>> Astrophysicists in Your Family?" (Gogol Bordello)
>>>>>
>>>>> The minimum is reached without steps, so... I don't know if the 
>>>>> single atom minimization wors or not...
>>>>>
>>>>
>>>> It certainly didn't work.  At the outset of the process, you 
>>>> instantly got a LINCS warning, indicating instability.  This would 
>>>> indicate to me that the model itself is unstable.  I'm sorry to say 
>>>> I don't know what to suggest, as the Gromos96 chloroform model 
>>>> should be rigid, and your topology reproduces what is in the force 
>>>> field .rtp entry for chloroform.
>>>>
>>>
>>> I guess I'm blaming this all on the topology, but it could also be 
>>> that your coordinates are messed up and they can't be resolved by 
>>> EM.  Check into that, as well.
>>>
>>> -Justin
>>>
>>> -- 
>>> ========================================
>>>
>>> Justin A. Lemkul
>>> Ph.D. Candidate
>>> ICTAS Doctoral Scholar
>>> MILES-IGERT Trainee
>>> Department of Biochemistry
>>> Virginia Tech
>>> Blacksburg, VA
>>> jalemkul[at]vt.edu | (540) 231-9080
>>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>>
>>> ========================================
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>>
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-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

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