[gmx-users] percentage of secondary structure

ahmet yıldırım ahmedo047 at gmail.com
Tue Jan 4 19:32:54 CET 2011


Dear Justin,

Thanks for your reply. There are not percentage of secondary structure in
"scount.xvg".
The content of the "scount.xvg" file are as the following:

# This file was created Tue Jan  4 19:28:47 2011
# by the following command:
# do_dssp -s run.tpr -f run.xtc
#
# do_dssp is part of G R O M A C S:
#
# GROup of MAchos and Cynical Suckers
#
@    title "Secondary Structure"
@    xaxis  label "Time (ps)"
@    yaxis  label "Number of Residues"
@TYPE xy
@ subtitle "Structure = A-Helix + B-Sheet + B-Bridge + Turn"
@ view 0.15, 0.15, 0.75, 0.85
@ legend on
@ legend box on
@ legend loctype view
@ legend 0.78, 0.8
@ legend length 2
@ s0 legend "Structure"
@ s1 legend "Coil"
@ s2 legend "B-Sheet"
@ s3 legend "B-Bridge"
@ s4 legend "Bend"
@ s5 legend "Turn"
@ s6 legend "A-Helix"
@ s7 legend "3-Helix"
       0   128    19    14     1    17    11   102     0
     0.2   131    19    14     1    14    14   102     0
     0.4   132    19    14     1    13    23    94     0
     0.6   130    19    14     1    15    12   103     0
     0.8   132    19    14     1    13    19    98     0
       1   130    21    14     1    13    28    87     0
.................................................................

04 Ocak 2011 20:04 tarihinde Justin A. Lemkul <jalemkul at vt.edu> yazdı:

>
>
> ahmet yıldırım wrote:
>
>> Dear Justin,
>>
>> I am using the commands as the following.
>> do_dssp -s run.tpr -f run.xtc
>> xpm2ps -f ss.xpm -o ss.eps
>>
>> You said "Have a look at the other output file do_dssp gives you". which
>> one should I look?
>>
>>
> Well, there's another output file from do_dssp called "scount.xvg," which
> should  be almost exactly what you need.  See do_dssp -h for a description,
> then look at the file yourself.
>
> -Justin
>
>  Thanks
>>
>> 04 Ocak 2011 15:51 tarihinde Justin A. Lemkul <jalemkul at vt.edu <mailto:
>> jalemkul at vt.edu>> yazdı:
>>
>>
>>
>>
>>    ahmet yıldırım wrote:
>>
>>        I get the residue versus time ( secondary structure graph) using
>>        do_dssp tool. I want to get the percentage value(alpha helix,
>>        beta sheet, coil ...) of secondary structure. For example:
>>
>>        Secondary structure percentage (%):
>>        Helix:51.6
>>        B-sheet:0
>>        Random coil:24.0
>>        Turn:24.4
>>
>>
>>    Have a look at the other output file do_dssp gives you.
>>
>>    -Justin
>>
>>
>>        04 Ocak 2011 14:59 tarihinde Mark Abraham
>>        <Mark.Abraham at anu.edu.au <mailto:Mark.Abraham at anu.edu.au>
>>        <mailto:Mark.Abraham at anu.edu.au
>>        <mailto:Mark.Abraham at anu.edu.au>>> yazdı:
>>
>>
>>           On 4/01/2011 11:38 PM, ahmet yıldırım wrote:
>>
>>               Dear Mark,
>>
>>               I will do by using the g_analyze tool but I do not know
>>            how to use it.
>>
>>
>>           There's a section in Appendix D of the manual for each tool,
>>        or you
>>           can use the -h argument to the tool to get the same information.
>>
>>           Either way, you haven't got the right tool. Hint: it's a
>> "protein
>>           specific analysis"
>>
>>           Mark
>>
>>
>>
>>               Thanks
>>
>>               2011/1/4 Mark Abraham <Mark.Abraham at anu.edu.au
>>            <mailto:Mark.Abraham at anu.edu.au>
>>               <mailto:Mark.Abraham at anu.edu.au
>>            <mailto:Mark.Abraham at anu.edu.au>>>
>>
>>
>>                   On 4/01/2011 8:33 PM, ahmet yıldırım wrote:
>>
>>                       Hi,
>>
>>                       How can I calculate the percentages (alpha helix,
>>            beta
>>                       sheet, coil ...) of secondary structure?
>>
>>
>>                   Look in manual section 7.4 for a suitable tool.
>>
>>                   Mark
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>>
>>               --     Ahmet YILDIRIM
>>
>>
>>
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>>
>>        --         Ahmet YILDIRIM
>>
>>
>>    --     ========================================
>>
>>    Justin A. Lemkul
>>    Ph.D. Candidate
>>    ICTAS Doctoral Scholar
>>    MILES-IGERT Trainee
>>    Department of Biochemistry
>>    Virginia Tech
>>    Blacksburg, VA
>>    jalemkul[at]vt.edu <http://vt.edu> | (540) 231-9080
>>
>>    http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>>
>>    ========================================
>>
>>    --     gmx-users mailing list    gmx-users at gromacs.org
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>>
>>
>>
>> --
>> Ahmet YILDIRIM
>>
>
> --
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> MILES-IGERT Trainee
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
> --
> gmx-users mailing list    gmx-users at gromacs.org
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-- 
Ahmet YILDIRIM
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