[gmx-users] Re: methanol simulation error

mustafa bilsel mfbilsel at gmail.com
Fri Jan 7 10:46:28 CET 2011


Hi Mark,
her is my topology,

best wishes
mustafa
;
;    File 'topol.top' was generated
;    By user: onbekend (0)
;    On host: onbekend
;    At date: Fri Jan  7 00:19:16 2011
;
;    This is a standalone topology file
;
;    It was generated using program:
;    pdb2gmx - VERSION 4.5.1
;
;    Command line was:
;    pdb2gmx -f vpgvg10.pdb -ignh
;
;    Force field was read from the standard Gromacs share directory.
;

; Include forcefield parameters
#include "gromos43a1.ff/forcefield.itp"

[ moleculetype ]
; Name            nrexcl
Protein             3

[ atoms ]
;   nr       type  resnr residue  atom   cgnr     charge       mass
typeB    chargeB      massB
; residue   1 VAL rtp VAL  q +1.0
     1         NL      1    VAL      N      1      0.129    14.0067   ; qtot
0.129
     2          H      1    VAL     H1      1      0.248      1.008   ; qtot
0.377
     3          H      1    VAL     H2      1      0.248      1.008   ; qtot
0.625
     4          H      1    VAL     H3      1      0.248      1.008   ; qtot
0.873
     5        CH1      1    VAL     CA      2      0.127     13.019   ; qtot
1
     6        CH1      1    VAL     CB      2          0     13.019   ; qtot
1
     7        CH3      1    VAL    CG1      2          0     15.035   ; qtot
1
     8        CH3      1    VAL    CG2      2          0     15.035   ; qtot
1
     9          C      1    VAL      C      3       0.38     12.011   ; qtot
1.38
    10          O      1    VAL      O      3      -0.38    15.9994   ; qtot
1
; residue   2 PRO rtp PRO  q  0.0
    11          N      2    PRO      N      4          0    14.0067   ; qtot
1
    12        CH1      2    PRO     CA      5          0     13.019   ; qtot
1
    13        CH2      2    PRO     CB      5          0     14.027   ; qtot
1
    14        CH2      2    PRO     CG      6          0     14.027   ; qtot
1
    15        CH2      2    PRO     CD      6          0     14.027   ; qtot
1
    16          C      2    PRO      C      7       0.38     12.011   ; qtot
1.38
    17          O      2    PRO      O      7      -0.38    15.9994   ; qtot
1
; residue   3 GLY rtp GLY  q  0.0
    18          N      3    GLY      N      8      -0.28    14.0067   ; qtot
0.72
    19          H      3    GLY      H      8       0.28      1.008   ; qtot
1
    20        CH2      3    GLY     CA      9          0     14.027   ; qtot
1
    21          C      3    GLY      C     10       0.38     12.011   ; qtot
1.38
    22          O      3    GLY      O     10      -0.38    15.9994   ; qtot
1
; residue   4 VAL rtp VAL  q  0.0
    23          N      4    VAL      N     11      -0.28    14.0067   ; qtot
0.72
    24          H      4    VAL      H     11       0.28      1.008   ; qtot
1
    25        CH1      4    VAL     CA     12          0     13.019   ; qtot
1
    26        CH1      4    VAL     CB     12          0     13.019   ; qtot
1
    27        CH3      4    VAL    CG1     12          0     15.035   ; qtot
1
    28        CH3      4    VAL    CG2     12          0     15.035   ; qtot
1
    29          C      4    VAL      C     13       0.38     12.011   ; qtot
1.38
    30          O      4    VAL      O     13      -0.38    15.9994   ; qtot
1
; residue   5 GLY rtp GLY  q  0.0
    31          N      5    GLY      N     14      -0.28    14.0067   ; qtot
0.72
    32          H      5    GLY      H     14       0.28      1.008   ; qtot
1
    33        CH2      5    GLY     CA     15          0     14.027   ; qtot
1
    34          C      5    GLY      C     16       0.38     12.011   ; qtot
1.38
    35          O      5    GLY      O     16      -0.38    15.9994   ; qtot
1
; residue   6 VAL rtp VAL  q  0.0
    36          N      6    VAL      N     17      -0.28    14.0067   ; qtot
0.72
    37          H      6    VAL      H     17       0.28      1.008   ; qtot
1
    38        CH1      6    VAL     CA     18          0     13.019   ; qtot
1
    39        CH1      6    VAL     CB     18          0     13.019   ; qtot
1
    40        CH3      6    VAL    CG1     18          0     15.035   ; qtot
1
    41        CH3      6    VAL    CG2     18          0     15.035   ; qtot
1
    42          C      6    VAL      C     19       0.38     12.011   ; qtot
1.38
    43          O      6    VAL      O     19      -0.38    15.9994   ; qtot
1
; residue   7 PRO rtp PRO  q  0.0
    44          N      7    PRO      N     20          0    14.0067   ; qtot
1
    45        CH1      7    PRO     CA     21          0     13.019   ; qtot
1
    46        CH2      7    PRO     CB     21          0     14.027   ; qtot
1
    47        CH2      7    PRO     CG     22          0     14.027   ; qtot
1
    48        CH2      7    PRO     CD     22          0     14.027   ; qtot
1
    49          C      7    PRO      C     23       0.38     12.011   ; qtot
1.38
    50          O      7    PRO      O     23      -0.38    15.9994   ; qtot
1
; residue   8 GLY rtp GLY  q  0.0
    51          N      8    GLY      N     24      -0.28    14.0067   ; qtot
0.72
    52          H      8    GLY      H     24       0.28      1.008   ; qtot
1
    53        CH2      8    GLY     CA     25          0     14.027   ; qtot
1
    54          C      8    GLY      C     26       0.38     12.011   ; qtot
1.38
    55          O      8    GLY      O     26      -0.38    15.9994   ; qtot
1
; residue   9 VAL rtp VAL  q  0.0
    56          N      9    VAL      N     27      -0.28    14.0067   ; qtot
0.72
    57          H      9    VAL      H     27       0.28      1.008   ; qtot
1
    58        CH1      9    VAL     CA     28          0     13.019   ; qtot
1
    59        CH1      9    VAL     CB     28          0     13.019   ; qtot
1
    60        CH3      9    VAL    CG1     28          0     15.035   ; qtot
1
    61        CH3      9    VAL    CG2     28          0     15.035   ; qtot
1
    62          C      9    VAL      C     29       0.38     12.011   ; qtot
1.38
    63          O      9    VAL      O     29      -0.38    15.9994   ; qtot
1
; residue  10 GLY rtp GLY  q -1.0
    64          N     10    GLY      N     30      -0.28    14.0067   ; qtot
0.72
    65          H     10    GLY      H     30       0.28      1.008   ; qtot
1
    66        CH2     10    GLY     CA     31          0     14.027   ; qtot
1
    67          C     10    GLY      C     32       0.27     12.011   ; qtot
1.27
    68         OM     10    GLY     O1     32     -0.635    15.9994   ; qtot
0.635
    69         OM     10    GLY     O2     32     -0.635    15.9994   ; qtot
0

[ bonds ]
;  ai    aj funct            c0            c1            c2            c3
    1     2     2    gb_2
    1     3     2    gb_2
    1     4     2    gb_2
    1     5     2    gb_20
    5     6     2    gb_26
    5     9     2    gb_26
    6     7     2    gb_26
    6     8     2    gb_26
    9    10     2    gb_4
    9    11     2    gb_9
   11    12     2    gb_20
   11    15     2    gb_20
   12    13     2    gb_26
   12    16     2    gb_26
   13    14     2    gb_26
   14    15     2    gb_26
   16    17     2    gb_4
   16    18     2    gb_9
   18    19     2    gb_2
   18    20     2    gb_20
   20    21     2    gb_26
   21    22     2    gb_4
   21    23     2    gb_9
   23    24     2    gb_2
   23    25     2    gb_20
   25    26     2    gb_26
   25    29     2    gb_26
   26    27     2    gb_26
   26    28     2    gb_26
   29    30     2    gb_4
   29    31     2    gb_9
   31    32     2    gb_2
   31    33     2    gb_20
   33    34     2    gb_26
   34    35     2    gb_4
   34    36     2    gb_9
   36    37     2    gb_2
   36    38     2    gb_20
   38    39     2    gb_26
   38    42     2    gb_26
   39    40     2    gb_26
   39    41     2    gb_26
   42    43     2    gb_4
   42    44     2    gb_9
   44    45     2    gb_20
   44    48     2    gb_20
   45    46     2    gb_26
   45    49     2    gb_26
   46    47     2    gb_26
   47    48     2    gb_26
   49    50     2    gb_4
   49    51     2    gb_9
   51    52     2    gb_2
   51    53     2    gb_20
   53    54     2    gb_26
   54    55     2    gb_4
   54    56     2    gb_9
   56    57     2    gb_2
   56    58     2    gb_20
   58    59     2    gb_26
   58    62     2    gb_26
   59    60     2    gb_26
   59    61     2    gb_26
   62    63     2    gb_4
   62    64     2    gb_9
   64    65     2    gb_2
   64    66     2    gb_20
   66    67     2    gb_26
   67    68     2    gb_5
   67    69     2    gb_5

[ pairs ]
;  ai    aj funct            c0            c1            c2            c3
    1     7     1
    1     8     1
    1    10     1
    1    11     1
    2     6     1
    2     9     1
    3     6     1
    3     9     1
    4     6     1
    4     9     1
    5    12     1
    5    15     1
    6    10     1
    6    11     1
    7     9     1
    8     9     1
    9    13     1
    9    14     1
    9    16     1
   10    12     1
   10    15     1
   11    17     1
   11    18     1
   12    19     1
   12    20     1
   13    17     1
   13    18     1
   14    16     1
   15    16     1
   16    21     1
   17    19     1
   17    20     1
   18    22     1
   18    23     1
   19    21     1
   20    24     1
   20    25     1
   21    26     1
   21    29     1
   22    24     1
   22    25     1
   23    27     1
   23    28     1
   23    30     1
   23    31     1
   24    26     1
   24    29     1
   25    32     1
   25    33     1
   26    30     1
   26    31     1
   27    29     1
   28    29     1
   29    34     1
   30    32     1
   30    33     1
   31    35     1
   31    36     1
   32    34     1
   33    37     1
   33    38     1
   34    39     1
   34    42     1
   35    37     1
   35    38     1
   36    40     1
   36    41     1
   36    43     1
   36    44     1
   37    39     1
   37    42     1
   38    45     1
   38    48     1
   39    43     1
   39    44     1
   40    42     1
   41    42     1
   42    46     1
   42    47     1
   42    49     1
   43    45     1
   43    48     1
   44    50     1
   44    51     1
   45    52     1
   45    53     1
   46    50     1
   46    51     1
   47    49     1
   48    49     1
   49    54     1
   50    52     1
   50    53     1
   51    55     1
   51    56     1
   52    54     1
   53    57     1
   53    58     1
   54    59     1
   54    62     1
   55    57     1
   55    58     1
   56    60     1
   56    61     1
   56    63     1
   56    64     1
   57    59     1
   57    62     1
   58    65     1
   58    66     1
   59    63     1
   59    64     1
   60    62     1
   61    62     1
   62    67     1
   63    65     1
   63    66     1
   64    68     1
   64    69     1
   65    67     1

[ angles ]
;  ai    aj    ak funct            c0            c1            c2
c3
    2     1     3     2    ga_9
    2     1     4     2    ga_9
    2     1     5     2    ga_10
    3     1     4     2    ga_9
    3     1     5     2    ga_10
    4     1     5     2    ga_10
    1     5     6     2    ga_12
    1     5     9     2    ga_12
    6     5     9     2    ga_12
    5     6     7     2    ga_14
    5     6     8     2    ga_14
    7     6     8     2    ga_14
    5     9    10     2    ga_29
    5     9    11     2    ga_18
   10     9    11     2    ga_32
    9    11    12     2    ga_30
    9    11    15     2    ga_30
   12    11    15     2    ga_20
   11    12    13     2    ga_12
   11    12    16     2    ga_12
   13    12    16     2    ga_12
   12    13    14     2    ga_12
   13    14    15     2    ga_12
   11    15    14     2    ga_12
   12    16    17     2    ga_29
   12    16    18     2    ga_18
   17    16    18     2    ga_32
   16    18    19     2    ga_31
   16    18    20     2    ga_30
   19    18    20     2    ga_17
   18    20    21     2    ga_12
   20    21    22     2    ga_29
   20    21    23     2    ga_18
   22    21    23     2    ga_32
   21    23    24     2    ga_31
   21    23    25     2    ga_30
   24    23    25     2    ga_17
   23    25    26     2    ga_12
   23    25    29     2    ga_12
   26    25    29     2    ga_12
   25    26    27     2    ga_14
   25    26    28     2    ga_14
   27    26    28     2    ga_14
   25    29    30     2    ga_29
   25    29    31     2    ga_18
   30    29    31     2    ga_32
   29    31    32     2    ga_31
   29    31    33     2    ga_30
   32    31    33     2    ga_17
   31    33    34     2    ga_12
   33    34    35     2    ga_29
   33    34    36     2    ga_18
   35    34    36     2    ga_32
   34    36    37     2    ga_31
   34    36    38     2    ga_30
   37    36    38     2    ga_17
   36    38    39     2    ga_12
   36    38    42     2    ga_12
   39    38    42     2    ga_12
   38    39    40     2    ga_14
   38    39    41     2    ga_14
   40    39    41     2    ga_14
   38    42    43     2    ga_29
   38    42    44     2    ga_18
   43    42    44     2    ga_32
   42    44    45     2    ga_30
   42    44    48     2    ga_30
   45    44    48     2    ga_20
   44    45    46     2    ga_12
   44    45    49     2    ga_12
   46    45    49     2    ga_12
   45    46    47     2    ga_12
   46    47    48     2    ga_12
   44    48    47     2    ga_12
   45    49    50     2    ga_29
   45    49    51     2    ga_18
   50    49    51     2    ga_32
   49    51    52     2    ga_31
   49    51    53     2    ga_30
   52    51    53     2    ga_17
   51    53    54     2    ga_12
   53    54    55     2    ga_29
   53    54    56     2    ga_18
   55    54    56     2    ga_32
   54    56    57     2    ga_31
   54    56    58     2    ga_30
   57    56    58     2    ga_17
   56    58    59     2    ga_12
   56    58    62     2    ga_12
   59    58    62     2    ga_12
   58    59    60     2    ga_14
   58    59    61     2    ga_14
   60    59    61     2    ga_14
   58    62    63     2    ga_29
   58    62    64     2    ga_18
   63    62    64     2    ga_32
   62    64    65     2    ga_31
   62    64    66     2    ga_30
   65    64    66     2    ga_17
   64    66    67     2    ga_12
   66    67    68     2    ga_21
   66    67    69     2    ga_21
   68    67    69     2    ga_37

[ dihedrals ]
;  ai    aj    ak    al funct            c0            c1
c2            c3            c4            c5
    2     1     5     9     1    gd_14
    1     5     6     7     1    gd_17
    1     5     9    11     1    gd_20
    5     9    11    12     1    gd_4
    9    11    12    16     1    gd_19
   12    11    15    14     1    gd_19
   11    12    13    14     1    gd_17
   11    12    16    18     1    gd_20
   12    13    14    15     1    gd_17
   13    14    15    11     1    gd_17
   12    16    18    20     1    gd_4
   16    18    20    21     1    gd_19
   18    20    21    23     1    gd_20
   20    21    23    25     1    gd_4
   21    23    25    29     1    gd_19
   23    25    26    27     1    gd_17
   23    25    29    31     1    gd_20
   25    29    31    33     1    gd_4
   29    31    33    34     1    gd_19
   31    33    34    36     1    gd_20
   33    34    36    38     1    gd_4
   34    36    38    42     1    gd_19
   36    38    39    40     1    gd_17
   36    38    42    44     1    gd_20
   38    42    44    45     1    gd_4
   42    44    45    49     1    gd_19
   45    44    48    47     1    gd_19
   44    45    46    47     1    gd_17
   44    45    49    51     1    gd_20
   45    46    47    48     1    gd_17
   46    47    48    44     1    gd_17
   45    49    51    53     1    gd_4
   49    51    53    54     1    gd_19
   51    53    54    56     1    gd_20
   53    54    56    58     1    gd_4
   54    56    58    62     1    gd_19
   56    58    59    60     1    gd_17
   56    58    62    64     1    gd_20
   58    62    64    66     1    gd_4
   62    64    66    67     1    gd_19
   64    66    67    69     1    gd_20

[ dihedrals ]
;  ai    aj    ak    al funct            c0            c1
c2            c3
    5     1     9     6     2    gi_2
    5     7     8     6     2    gi_2
    9     5    11    10     2    gi_1
   11     9    12    15     2    gi_1
   12    11    16    13     2    gi_2
   16    12    18    17     2    gi_1
   18    16    20    19     2    gi_1
   21    20    23    22     2    gi_1
   23    21    25    24     2    gi_1
   25    23    29    26     2    gi_2
   25    27    28    26     2    gi_2
   29    25    31    30     2    gi_1
   31    29    33    32     2    gi_1
   34    33    36    35     2    gi_1
   36    34    38    37     2    gi_1
   38    36    42    39     2    gi_2
   38    40    41    39     2    gi_2
   42    38    44    43     2    gi_1
   44    42    45    48     2    gi_1
   45    44    49    46     2    gi_2
   49    45    51    50     2    gi_1
   51    49    53    52     2    gi_1
   54    53    56    55     2    gi_1
   56    54    58    57     2    gi_1
   58    56    62    59     2    gi_2
   58    60    61    59     2    gi_2
   62    58    64    63     2    gi_1
   64    62    66    65     2    gi_1
   67    66    69    68     2    gi_1





; Include Position restraint file
#ifdef POSRES
#include "posre.itp"
#endif

; Include water topology
#include "gromos43a1.ff/methanol.itp"

[ system ]
; Name
Protein in methanol

[ molecules ]
; Compound        #mols
Protein             1
MTH               660


Message: 7
Date: Fri, 07 Jan 2011 12:41:27 +1100
From: Mark Abraham <Mark.Abraham at anu.edu.au>
Subject: Re: [gmx-users] methanol simulation error
To: Discussion list for GROMACS users <gmx-users at gromacs.org>
Message-ID: <4D266F47.5000004 at anu.edu.au>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

On 7/01/2011 12:31 PM, mustafa bilsel wrote:
> Hi all,
> I try to make a simulation in methanol. I use gromos43a1 forcefield.
> My command follow,
> pdb2gmx -f xxx.pdb
> editconf -f conf.gro -bt dodecahedron -d 0.8 -o box.gro
> genbox -cp box.gro -cs methanol216.gro -p topol.top -o solvated.gro
> When  grompp for minimization it gives an error:
> Atomtype CMET not found
>
> I have used grep to count the methanol molecules and added SOL 660
> comment in .top file to tell the number of methanols.
> Also I added #include "gromos43a1.ff/methanol.itp" line to .top.

I'm not sure why I'm repeating myself, but the name given in that
moleculetype is not SOL. That name must match what appears in the
[molecules] section.

Please make sure you have read the examples in Chapter 5 of the manual
and understand everything there.

>
> Please don't suggest non-water solvation in how-to part of Gromacs web.
>
> Could you tell me what missing is?

You're using atomtype CMET somewhere in your .top or its #included .itp
files. Your forcefield doesn't define atomtype CMET, and it needs to if
your usage is correct. Without seeing your topology, we can't say more.

Mark
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