[gmx-users] fatal error

Justin A. Lemkul jalemkul at vt.edu
Sat Jan 22 21:54:49 CET 2011



ahmet yıldırım wrote:
> Dear Justin,
> 
> I manually specify termini.  I choosed "None" but I have the same error.
> 
> Fatal error:
> atom C not found in residue 13NH2 while combining tdb and rtp
> 

Please post your .pdb file.

-Justin

> 
> 22 Ocak 2011 00:45 tarihinde Justin A. Lemkul <jalemkul at vt.edu 
> <mailto:jalemkul at vt.edu>> yazdı:
> 
> 
> 
>     ahmet yıldırım wrote:
> 
>         Dear justin,
> 
>         I looked at the pdb file. No NH2 reside contains carbon atom.
> 
>         What should I do?
> 
> 
>     In this case, you need to manually specify termini.  Choose "None"
>     to allow your capping groups to be built properly.
> 
>     -Justin
> 
> 
>         22 Ocak 2011 00:18 tarihinde Justin A. Lemkul <jalemkul at vt.edu
>         <mailto:jalemkul at vt.edu> <mailto:jalemkul at vt.edu
>         <mailto:jalemkul at vt.edu>>> yazdı:
> 
> 
> 
> 
>            ahmet yıldırım wrote:
> 
>                I think I need to edit the aminoacids.rtp file in
>         gromos43a1.ff
>                folder as far as I understand from mailing list. I am
>         using 43a1
>                forcefield. I do not understand what kind of changes
>         should I do.
> 
> 
>            You do not need to modify the .rtp file, you need to modify your
>            coordinate file, as I said before.  The fatal error indicates
>         that
>            you have included a C atom in an NH2 residue, which is just a
>            neutral amine and contains no carbon, as should be clear from the
>            .rtp file.
> 
>            -Justin
> 
>                *the aminoacids.rtp file:*
> 
>                [ ACE ]
>                 [ atoms ]
>                   CA   CH3   0.000     0
>                    C     C   0.380     1
>                    O     O  -0.380     1
>                 [ bonds ]
>                    C    CA   gb_26
>                    C     O   gb_4
>                    C    +N   gb_9
>                 [ angles ]
>                  CA     C     O    ga_30
>                  CA     C    +N    ga_18
>                   O     C    +N    ga_32
>                 [ impropers ]
>                   C    CA    +N     O    gi_1
> 
>                [ NH2 ]
>                 [ atoms ]
>                    N    NT   -0.83    0
>                    H1    H   0.415    0
>                    H2    H   0.415   0
>                 [ bonds ]
>                     N    H1  gb_2
>                     N    H2  gb_2       -C    N   gb_8
>                 [ angles ]
>                    -O -C N  ga_32
>                    -CA -C N ga_18
>                    -C N H1  ga_22
>                    -C N H2  ga_22
>                    H1 N H2  ga_23
>                 [ dihedrals ]
>                   -CA -C N H1 gd_4
>                 [ impropers ]
>                   -C -O N -CA gi_1
>                    N  H1 H2  -C gi_1
> 
>                [ ALA ]
>                 [ atoms ]
>                   N     N    -0.28000     0
>                   H     H     0.28000     0
>                  CA   CH1     0.00000     1
>                  CB   CH3     0.00000     1
>                   C     C       0.380     2
>                   O     O      -0.380     2
>                 [ bonds ]
>                   N     H    gb_2       N    CA    gb_20     CA     C  
>          gb_26
>                     C     O    gb_4       C    +N    gb_9      CA    CB
>            gb_26          2011/1/21 Justin A. Lemkul <jalemkul at vt.edu
>         <mailto:jalemkul at vt.edu>
>                <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>>
>         <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>
> 
>                <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>>>>
> 
> 
> 
> 
>                   ahmet yıldırım wrote:
> 
>                       Dear users,
> 
>                       Gromacs 4.5.3
>                       pdb2gmx -f xxx.pdb -water spc
>                       select Force Field:9
>                       *Fatal error:*
>                       atom C not found in buiding block 13NH2 while
>         combining
>                tdb and rtp
>                       For more information and tips for troubleshooting,
>         please
>                check
>                       the GROMACS
>                       website at http://www.gromacs.org/Documentation/Errors
> 
> 
>                       How can I fixed this error?
> 
> 
>                   You have a carbon atom in a residue that should only
>         contain NH2.
>                    Refer to the .rtp file for what is expected, then make a
>                suitable
>                   structure that conforms to those requirements.
> 
>                   This has been asked and answered hundreds of times, so
>         please
>                make
>                   use of the mailing list search.  You would have gotten
>         your
>                answer
>                   in minutes rather than hours.
> 
>                   -Justin
> 
> 
>                       Thanks in advance
> 
> 
>                       --         Ahmet YILDIRIM
> 
> 
>                   --     ========================================
> 
>                   Justin A. Lemkul
>                   Ph.D. Candidate
>                   ICTAS Doctoral Scholar
>                   MILES-IGERT Trainee
>                   Department of Biochemistry
>                   Virginia Tech
>                   Blacksburg, VA
>                   jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu>
>         <http://vt.edu> | (540)
> 
>                231-9080
> 
>                   http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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> 
> 
>                --         Ahmet YILDIRIM
> 
> 
>            --     ========================================
> 
>            Justin A. Lemkul
>            Ph.D. Candidate
>            ICTAS Doctoral Scholar
>            MILES-IGERT Trainee
>            Department of Biochemistry
>            Virginia Tech
>            Blacksburg, VA
>            jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu> | (540)
>         231-9080
>            http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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> 
> 
>         -- 
>         Ahmet YILDIRIM
> 
> 
>     -- 
>     ========================================
> 
>     Justin A. Lemkul
>     Ph.D. Candidate
>     ICTAS Doctoral Scholar
>     MILES-IGERT Trainee
>     Department of Biochemistry
>     Virginia Tech
>     Blacksburg, VA
>     jalemkul[at]vt.edu <http://vt.edu> | (540) 231-9080
>     http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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> 
> 
> -- 
> Ahmet YILDIRIM

-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

========================================



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