[gmx-users] Domain decomposition error in alchemical free energy perturbation MD

Xiaoxiao He xxiaohe at ust.hk
Sat Oct 15 08:11:17 CEST 2011


Dear all,

I'm doing an "slow-growth" alchemical free energy perturbation calculation
of the formation of a disulfide bridge between two Cysteines with Gromacs.

I've had tried different ways to combine the topology of both state A and
state B, and finally settled with the most direct way -- to "mutate" the
atoms that have different partial charges in the two states, and transform
the two hydrogen atoms into "dummy" atoms. As suggested by Gromacs manual
and some messages from the internet, I put explicitly the OPLS parameters
for the bonds, pairs, angles and dihedrals changed from state A to state B,
and the grompp didn't give a warning. But when I was testing the production
simulation on two processors, there was a warning of fatal error in the log
file:

=========================================================================================
Initializing Domain Decomposition on 2 nodes
Dynamic load balancing: auto
Will sort the charge groups at every domain (re)decomposition
Initial maximum inter charge-group distances:
    two-body bonded interactions: 3.774 nm, LJC Pairs NB, atoms 12 205
  multi-body bonded interactions: 0.640 nm, Ryckaert-Bell., atoms 1013 417
Minimum cell size due to bonded interactions: 4.151 nm
Using 0 separate PME nodes
Scaling the initial minimum size with 1/0.8 (option -dds) = 1.25
Optimizing the DD grid for 2 cells with a minimum initial size of 5.189 nm
The maximum allowed number of cells is: X 0 Y 0 Z 0

-------------------------------------------------------
Program mdrun, VERSION 4.5.4
Source code file: domdec.c, line: 6436

Fatal error:
There is no domain decomposition for 2 nodes that is compatible with the
given box and a minimum cell size of 5.18876 nm
Change the number of nodes or mdrun option -rdd or -dds
Look in the log file for details on the domain decomposition
For more information and tips for troubleshooting, please check the GROMACS
website at http://www.gromacs.org/Documentation/Errors

=============================================================================================

The atoms indexed 12 and 205 are a CD1 in Leu1 and another CB in Ala12
respectively. I checked the topology and found no entries containing both
these atoms. What's weird is that there's no way for these two atoms to have
bonded interactions, but it says in the log that they have a two-body bonded
interaction with a distance of 3.774 nm, which I cannot understand.

Does anyone have an explanation what this could mean? Any suggestion is
appreciated!

Thanks in advance!


Xiaoxiao He
Oct. 15, 2011



Attached are the input  parameters for the production md:
=====================================================================
-Input Parameters:
   integrator           = sd
   nsteps               = 2000000
   init_step            = 0
   ns_type              = Grid
   nstlist              = 10
   ndelta               = 2
   nstcomm              = 10
   comm_mode            = Linear
   nstlog               = 100
   nstxout              = 100000
   nstvout              = 100000
   nstfout              = 100000
   nstcalcenergy        = 10
   nstenergy            = 100
   nstxtcout            = 1000
   init_t               = 0
   delta_t              = 0.0005
   xtcprec              = 1000
   nkx                  = 36
   nky                  = 36
   nkz                  = 36
   pme_order            = 4
   ewald_rtol           = 1e-05
   ewald_geometry       = 0
   epsilon_surface      = 0
   optimize_fft         = TRUE
   ePBC                 = xyz
   bPeriodicMols        = FALSE
   bContinuation        = FALSE
   bShakeSOR            = FALSE
   etc                  = No
   nsttcouple           = -1
   epc                  = Berendsen
   epctype              = Isotropic
   nstpcouple           = 10
   tau_p                = 1
   ref_p (3x3):
      ref_p[    0]={ 1.00000e+00,  0.00000e+00,  0.00000e+00}
      ref_p[    1]={ 0.00000e+00,  1.00000e+00,  0.00000e+00}
      ref_p[    2]={ 0.00000e+00,  0.00000e+00,  1.00000e+00}
   compress (3x3):
      compress[    0]={ 4.50000e-05,  0.00000e+00,  0.00000e+00}
      compress[    1]={ 0.00000e+00,  4.50000e-05,  0.00000e+00}
      compress[    2]={ 0.00000e+00,  0.00000e+00,  4.50000e-05}
   refcoord_scaling     = No
   posres_com (3):
      posres_com[0]= 0.00000e+00
      posres_com[1]= 0.00000e+00
      posres_com[2]= 0.00000e+00
   posres_comB (3):
      posres_comB[0]= 0.00000e+00
      posres_comB[1]= 0.00000e+00
      posres_comB[2]= 0.00000e+00
   andersen_seed        = 815131
   rlist                = 1.3
   rlistlong            = 1.3
   rtpi                 = 0.05
   coulombtype          = PME
   rcoulomb_switch      = 0
   rcoulomb             = 1.3
   vdwtype              = Switch
   rvdw_switch          = 0.8
   rvdw                 = 0.9
   epsilon_r            = 1
   epsilon_rf           = 1
   tabext               = 1
   implicit_solvent     = No
   gb_algorithm         = Still
   gb_epsilon_solvent   = 80
   nstgbradii           = 1
   rgbradii             = 1
   gb_saltconc          = 0
   gb_obc_alpha         = 1
   gb_obc_beta          = 0.8
   gb_obc_gamma         = 4.85
   gb_dielectric_offset = 0.009
   sa_algorithm         = Ace-approximation
   sa_surface_tension   = 2.05016
   DispCorr             = EnerPres
   free_energy          = yes
   init_lambda          = 0
   delta_lambda         = 5e-07
   n_foreign_lambda     = 0
   sc_alpha             = 0.5
   sc_power             = 1
   sc_sigma             = 0.3
   sc_sigma_min         = 0.3
   nstdhdl              = 10
   separate_dhdl_file   = no
   dhdl_derivatives     = yes
   dh_hist_size         = 0
   dh_hist_spacing      = 0.1
   nwall                = 0
   wall_type            = 9-3
   wall_atomtype[0]     = -1
   wall_atomtype[1]     = -1
   wall_density[0]      = 0
   wall_density[1]      = 0
   wall_ewald_zfac      = 3
   pull                 = no
   disre                = No
   disre_weighting      = Conservative
   disre_mixed          = FALSE
   dr_fc                = 1000
   dr_tau               = 0
   nstdisreout          = 100
   orires_fc            = 0
   orires_tau           = 0
   nstorireout          = 100
   dihre-fc             = 1000
   em_stepsize          = 0.01
   em_tol               = 10
   niter                = 20
   fc_stepsize          = 0
   nstcgsteep           = 1000
   nbfgscorr            = 10
   ConstAlg             = Lincs
   shake_tol            = 0.0001
   lincs_order          = 4
   lincs_warnangle      = 30
   lincs_iter           = 1
   bd_fric              = 0
   ld_seed              = 1993
   cos_accel            = 0
   deform (3x3):
      deform[    0]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
      deform[    1]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
      deform[    2]={ 0.00000e+00,  0.00000e+00,  0.00000e+00}
   userint1             = 0
   userint2             = 0
   userint3             = 0
   userint4             = 0
   userreal1            = 0
   userreal2            = 0
   userreal3            = 0
   userreal4            = 0
grpopts:
   nrdf:        3903       22866
   ref_t:         300         300
   tau_t:         0.1         0.1
anneal:          No          No
ann_npoints:           0           0
   acc:           0           0           0
   nfreeze:           N           N           N
   energygrp_flags[  0]: 0
   efield-x:
      n = 0
   efield-xt:
      n = 0
   efield-y:
      n = 0
   efield-yt:
      n = 0
   efield-z:
      n = 0
   efield-zt:
      n = 0
   bQMMM                = FALSE
   QMconstraints        = 0
   QMMMscheme           = 0
   scalefactor          = 1
qm_opts:
   ngQM                 = 0

======================================================================================================


-- 
Xiaoxiao HE
Department of Chemistry
The Hong Kong University of Science and Technology
Clear Water Bay, Kowloon
Hong Kong

Email: xxiaohe at ust.hk     Tel: 23585896
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://maillist.sys.kth.se/pipermail/gromacs.org_gmx-users/attachments/20111015/037dc0f7/attachment.html>


More information about the gromacs.org_gmx-users mailing list